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Inhibitory effects of 1-methyl-4-phenylpyridinium on glutamate uptake into cultured C6 glioma cells

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Acta Pharmacologica Sinica 2004年第7期 9-14页

作者： hong-hong YAO, Jian-hua DING, Hai-rong HE, Gang HU2 Department of Pharmacologyand Neurobiology, Nanjing Medical University, Nanjing 210029, China

AIM: To investigate the effect of 1-methyl-4-phenylpyridinium (MPP+) on the glutamate uptake into cultured C6 glioma cells. METHODS: The glutamate uptake into C6 glioma cells was measured by radio-ligand binding assay method. The effect of MPP+ on the morphology of C6 glioma cells was observed under phase contrast microscopy; apoptosis of C6 glioma cells were measured by FITC-labeled Annexin V staining and flow cytometry. Cell viability was measured by MTT method. RESULTS: MPP+inhibited glutamate uptake into C6 glioma cells. However, MPP+ failed to induce any morphological changes of C6 glioma cells, and exposure to MPP+ had no effect on the viability and the apoptotic percentage of C6 glioma cells. Incubation with 12-O-tetradecanoylphorbol -13-acetate (TPA), a protein kinase C activator, caused a significant increase in glutamate uptake and completely reversed MPP+-induced inhibitory effect on glutamate uptake. CONCLUSION: The present results indicate that glutamate transporters mayhave important pathogeneticimplications in Parkinson disease. MPP+-inducedinhibition ofglutamate uptake was due to the dysfunction of glutamate transporters; TPA enhanced glutamate uptake and completely reversed the inhibitory effect of MPP+.

关键词： methyl-4-phenylpyridinium glutamate acid glioma apoptosis 4-O-methyl-12-O-tetradecanoyl- phorbol-13-acetate

Preparations Continue for '95 NGO Forum

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Women of China 1995年第6期 34-35页

作者： hong hua XIAOhong LIU

AFTER a year of preparation, China is finally ready to host 42 seminars sponsored by 45 Chinese non-governmental organizations during the ’95 NGO Forum to be held at the same time of the UN Fourth World Conference on Women. The wide range of topics concerning women include employment, health, education, politics, legislation, culture, childhood, adulthood and aging, ethnic nationalities, the disabled, human rights, domestic violence, peace, etc. Papers scheduled to be read during the forum are currently being reviewed.

关键词：非政府组织 Forum Chinese 英文 China establishment Translate have ranging gather

Stroke Prone Renovascular Hypertensive Rat: An Effective Animal Model for Researches on Stroke Triggered by Cold Wave

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Stroke Prone Renovascular Hypertensive Rat: An Effective Ani...

Effect of parenteral glutamine supplementation in premature infants

2021海峡两岸暨港澳精准医学青年博士论坛

作者： Zhong Kaiyi hong hua Department of Neurology the First Affiliated Hospital of Sun Yat-sen University

Epidemiological data and clinical observations have shown that cold wave maybe a trigger to the stroke. In recent years, more and more studies have used the stroke prone renovascular hypertensive rats(RHRSP) model under the artificial cold wave to explore the relationship between cold wave and stroke. Pervious researches show that stroke triggered by cold wave may relate multiple mechanisms such as blood pressure, coagulation and fibrinolysis system, vascular endothelial cell function and inflammation. In this review, we will discuss the progress of RHRSP cold wave model on stroke onset.

关键词： RHRSP cold wave stroke onset

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Chinese Medical Journal 2007年第2期120卷 140-144页

作者： LI Zheng-hong WANG Dan-hua DONG Mei Department of Paediatrics Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100730 China

Background Glutamine, proposed to be conditionally essential for critically ill patients, is not added routinely to parenteral amino acid formulations for premature infants and is provided in only small quantities by the enteral route when enteral feeding is low. Parenteral feeding is the basic way of nutrition in the first days of life of premature infants. In this study, we evaluated the effects of glutamine supplemented parenteral nutrition for premature infants on growth and development, feeding toleration, and infective episodes. Methods From December 2002 to July 2006, 53 premature infants were given either standard or glutamine supplemented parenteral nutrition for more than 2 weeks. Twenty-eight infants were in glutamine supplemented group, whose gestational age （31.4±2.0） weeks, birth weight range （1386±251） g; twenty-five infants were in control group, gestational age （31.1±1.7） weeks, with birth weight range （1346±199） g. There were no differences between the two groups. Various growth and biochemical indices were monitored throughout the duration of hospital stay. Data between groups were analyzed with Student＇s t test. Nonparametric data were analyzed using a Chi-square test. A two-tailed P value 〈 0.05 was considered statistically significant. Results The level of serum albumin was lower in the glutamine groups on the second week （3.0 vs 3.2 g/dl,P=-0.028）, and blood urea nitrogen was higher in glutamine groups on the fourth week （8.1 vs 4.9 mg/dl, P=0.014）, but normal. Glutamine group infants took fewer days to regain birth weight （8.1 vs 10.4 days, P=0.017）, required fewer days on parenteral nutrition （24.8 vs 30.8 days, P=0.035）, with shorter stays in hospital （32.1 vs 38.6 days, P=0.047）. Episodes of hospital acquired infection in glutamine supplemented infants were lower than that in control group （0.96 vs 1.84 times, P=0.000）. Conclusion Parenteral glutamine supplementation in premature infants can shorten days on parenteral nutrition and l

关键词： glutamine parenteral nutrition premature infant

Effects of Inhibiting JAK on Invasion and Metastasis of the Human Breast Cancer Cells through ERK Signaling Transduction Pathway

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Chinese Journal of Cancer Research 2009年第1期21卷 32-36页

作者： Jing Zhao hong-fang Chen hua-yu Deng Department of Pathophysiology Chongqing Medical University Chonging 400016 China

Objective： To explore the effects of Janus activated kinase （JAK） inhibitor AG490 on the phosphorylation of extracellular signal regulated protein kinase （ERK） in human breast cancer cells MDA-MB-231 and the roles of JAK in the invasion and metastasis of the human breast cancer cells through ERK signaling transduction pathways. Methods： MDA-MB-231 cells were treated with 20 μmol/L, 40 μmol/L, 80 μmol/L Janus kinase inhibitor AG490 for 24, 48 and 72 h. Proliferation and adhesion of MDA-MB-231 cells to matrigel were measured with MTT assay. When treated with 40 μmol/L AG490 for 24 h, the expressions of P-ERK and MMP-9 of cells were detected by Western-blot and invasion and metastasis of MDA-MB-231 cells were evaluated with transwell chamber. Results： After being treated with 20 μmol/L, 40 μ/L, 80 μmol/L AG490 for 24, 48 and 72 h, the proliferation of MDA-MB-231 cells was inhibited in a dose-and time-dependent manner. MDA-MB-231 cells treated with 40 μmol/L AG490 for 30, 60, 90 and 120 rain resulted in the increasing adhesion of cells to Matrigel in a time-dependent manner. However, capacity of adhesion in the group treated with AG490 was significantly decreased in comparison with the control group （P〈0.01）. The expression level of P-ERK and MMP-9 were decreased when treated with AG490. After treatment with 40 μmol/L AG490, in invasion assay, the number of cells in AG490 treated group to migrate to filter coated with Matrigel was reduced compared with control group （P〈0.05） Meanwhile, in migration assay, the number of cells in AG490 treated group to migrate to filter was also decreased compared with control group （P〈0.05）. Conclusion： Our study indicates that JAK kinase could affect the activity of ERK signal transduction pathway through the phosphorylation of ERK. The inhibitory effects of JAK kinase on MMP-9 expression and invasion of breast cancer cells were associated with the down-regulation of the ERK signaling pathway.

关键词： Breast cancer JAK ERK Invasion Metastasis

Frequency characteristic of series piezoelectric sensor and application to the determination of water in organic solvents

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Chinese Journal of Chemistry 1994年第1期12卷 58-65页

作者： SHEN, Da-Zhong ZHU, Wen-hong NIE, Li-hua YAO, Shou-Zhou New Material Research Institute, Hunan University, Changsha, Hunan 410082, China New Material Research Institute Hunan University Changsha Hunan 410082 China

Theoretical consideration was proposed for the frequency response of a series piezoelectric sensor to the properties of liquid and verified experimentally. This kind of sensor has sensitive and selective frequency response to the permittivity and specific conductivity of solution with excellent stability, and was applied to the determination of micro-content of water in organic solvents. The detection limit is 0.01 g/L in dioxane.

关键词： Piezoelectric quartz crystal chemical sensor micro-water-content

Wing patterning genes of Nilaparvata lugens identification by transcriptome analysis, and their differential expression profile in wing pads between brachypterous and macropterous morphs

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Journal of Integrative Agriculture 2015年第9期14卷 1796-1807页

作者： LI Kai-yin HU Ding-bang LIU Fang-zhou LONG Man LIU Si-yi ZHAO Jing HE Yue-ping hua hong-xia Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural University

The brown planthopper, Nilaparvata lugens is an economically important pest on rice plants. This species produces macropterous and brachypterous morphs in response to environmental cues, which makes it very dififcult to control. The molecular basis of wing patterning in N. lugens is stil unknown. It is necessary to identify wing patterning genes of N. lugens, and also to clarify the expression differences of wing patterning genes between macropterous and brachypter-ous morphs. High-throughput deep sequencing of transcriptome of N. lugens wing pad yielded 116 744 580 raw reads and 113 042 700 clean reads. Al the reads were assembled into 55 963 unigenes with an average length of 804 bp. With the E-value cut-off of 1.0E–5,18 359 and 2 883 unigens had hits in NCBI-NR (NCBI non-redundant protein sequences) and NCBI-NT (NCBI nucleotide sequences) databases, respectively. A total of 16 502 unigenes were assigned to GO (gene ontology) classiifcation, 9 709 ungenes were grouped into 26 COG (cluster of orthologous groups of proteins) classiifcations, and 6 724 unigenes were assigned to different KEGG (Kyoto encyclopedia of genes and genomes) path-ways. In total, 56 unigenes which are homologous to wing patterning genes of Drosophila melanogaster or Tribolium castaneum were identiifed. Out of the 56 unigenes, 24 unigenes were selected, and their expression levels across the ifve nymphal stages between macropterous strain and brachypterous strain were examined by qRT-PCR. Two-way ANOVA analysis showed that development stage had signiifcant effects on the expression level of al the 24 genes (P<0.05). The expression levels of 8 genes (Nlen, Nlhh, Nlsal, NlAbd-A, Nlwg, Nlvg, Nlexd and NlUbx) were signiifcantly affected by wing morph. This is the ifrst transcriptome analysis of wing pads of hemimetabolous insect, N. lugens. The identiifed wing patterning genes would be useful resource for future exploration of molecular basis of wing development. The 8 differential y expressed win

关键词： Nilaparvata lugens wing pad transcriptome wing patterning genes

Efficacy and safety of minimally invasive laparoscopic surgery under general anesthesia for ovarian cancer

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World Journal of Clinical Cases 2024年第9期12卷 1569-1577页

作者： Xian Qin Chen Chen Yang Liu Xian-hong hua Jia-Yi Li Meng-Jie Liang Fang Wu Department of Anesthesiology The First People's Hospital of Jiangxia DistrictWuhan 430200Hubei ProvinceChina Department of Obstetrics and Gynecology The First People's Hospital of Jiangxia DistrictWuhan 430200Hubei ProvinceChina

BACKGROUND Ovarian cancer is one of the most common malignant tumors in female reproductive system in the world,and the choice of its treatment is very important for the survival rate and prognosis of *** open surgery is the main treatment for ovarian cancer,but it has the disadvantages of big trauma and slow *** the continuous development of minimally invasive technology,minimally invasive laparoscopic surgery under general anesthesia has been gradually applied to the treatment of ovarian cancer because of its advantages of less trauma and quick ***,the efficacy and safety of minimally invasive laparoscopic surgery under general anesthesia in the treatment of ovarian cancer are still *** To explore the efficacy and safety of general anesthesia minimally invasive surgery in the treatment of ovarian *** The clinical data of 90 patients with early ovarian cancer in our hospital were analyzed *** to the different surgical treatment methods,patients were divided into study group and control group(45 cases in each group).The study group received minimally invasive laparoscopic surgery under general anesthesia for ovarian cancer,while the control group received traditional open surgery for ovarian *** European Organization for Research and Treatment of Cancer Quality of Life Questionnaire(EORTC QLQ-C30),clinical efficacy and safety of the two groups were *** The intraoperative blood loss,length of hospital stay,postoperative gas evacuation time,and postoperative EORTC QLQ-C30 score of the study group were significantly better than those of the control group(P<0.05).The incidence of postoperative complications in the study group was significantly lower than in the control group(P<0.05).The two groups had no significant differences in the preoperative adrenocorticotropic hormone(ACTH),androstenedione(AD),cortisol(Cor),cluster of differentiation 3 positive(CD3+),and clu

关键词： Early-stage ovarian cancer Efficacy Minimally invasive Laparoscopy Safety Surgery

An element-free Galerkin method for ground penetrating radar numerical simulation

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Journal of Central South University 2015年第1期22卷 261-269页

作者：冯德山郭荣文王洪华 School of Geosciences and Info-Physics Central South University Key Laboratory of Metallogenic Prediction of Nonferrous Metals Ministry of Education(Central South University)

An element-free Galerkin method(EFGM) is used to solve the two-dimensional(2D) ground penetrating radar(GPR)modelling problems, due to its simple pre-processing, the absence of elements and high accuracy. Different from element-based numerical methods, this approach makes nodes free from the elemental restraint and avoids the explicit mesh discretization. First, we derived the boundary value problem for the 2D GPR simulation problems. Second, a penalty function approach and a boundary condition truncated method were used to enforce the essential and the absorbing boundary conditions, respectively. A three-layered GPR model was used to verify our element-free approach. The numerical solutions show that our solutions have an excellent agreement with solutions of a finite element method(FEM). Then, we used the EFGM to simulate one more complex model to show its capability and limitations. Simulation results show that one obvious advantage of EFGM is the absence of element mesh, which makes the method very flexible. Due to the use of MLS fitting, a key feature of EFM, is that both the dependent variable and its gradient are continuous and have high precision.

关键词： element-free Galerkin method moving least-squares method ground penetrating radar forward simulation