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自固定自脱型胰肠吻合引流支架应用于胰肠吻合术的动物实验研究

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中华外科杂志 2024年第7期62卷 697-702页

作者：刘小龙蒋桂星张一卓董昕俣洪德飞浙江大学医学院附属邵逸夫医院普外科杭州310016 浙江大学医学院杭州310016

目的评估自固定自脱型胰肠吻合引流支架应用于胰肠吻合术的安全性和有效性。方法本研究为动物实验研究。基于胰肠吻合"瘘管愈合"学说创建的洪氏胰肠吻合技术研制自固定自脱型胰肠吻合引流支架,动物实验前已完成10项生物相容性体外试验... 详细信息

目的评估自固定自脱型胰肠吻合引流支架应用于胰肠吻合术的安全性和有效性。方法本研究为动物实验研究。基于胰肠吻合"瘘管愈合"学说创建的洪氏胰肠吻合技术研制自固定自脱型胰肠吻合引流支架,动物实验前已完成10项生物相容性体外试验。实验于2022年2—9月完成。取25只普通级巴马小型猪,采用丝线双重结扎胰腺颈部,使结扎远端主胰管扩张建模。将建模成功的23只巴马小型猪采用分层随机法分为三组,即支架组(11只)、手工缝合组(8只)、假手术组(4只)。比较支架组和手工缝合组的胰肠吻合时间、术后腹腔引流液淀粉酶浓度及胰肠吻合口承受的压力值。通过腹部X线片观察自固定自脱型胰肠吻合引流支架脱落时间。通过术后病理学检查观察胰肠吻合口愈合时间、愈合方式和狭窄率。采用独立样本t检验分析定量资料;采用Fisher确切概率法分析分类资料。结果支架组和手工缝合组的胰管直径和胰腺质地、胰肠吻合时间、术后腹腔引流液淀粉酶浓度和胰肠吻合口承受压力值的差异均无统计学意义(P值均>0.05)。腹部X线检查结果显示,引流支架组术后21 d胰肠吻合引流支架逐渐脱落排除体外,术后3个月后支架均脱落。大体和镜下病理学检查结果显示,支架组术后7 d吻合口愈合;手工缝合组术后14 d吻合口愈合。支架组和手工缝合组术后35 d内的吻合口狭窄率分别为2/8和6/8(Fisher确切概率法:P=0.132)。结论自固定自脱型胰肠吻合引流支架可安全有效地用于巴马小型猪的胰肠吻合术中,与手工缝合法胰肠吻合技术相比,吻合口愈合时间可能更早,狭窄率可能更低。

关键词：外科手术手术后并发症胰肠吻合瘘管愈合吻合口狭窄动物实验

Regulation of BMP2K in AP2M1-mediated EGFR internalization during the development of gallbladder cancer

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1134-1136页

作者： Xiaoling Song Maolan Li Wenguang Wu Wei Dang Yuan Gao Rui Bian Runfa Bao Yunping Hu defei hong Jun Gu Yingbin Liu Department of General Surgery and Laboratory of General Surgery Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine1665 Kongjiang Road200092 ShanghaiChina Shanghai Key Laboratory of Biliary Tract Disease Research Shanghai Jiao Tong University200127 ShanghaiChina State Key Laboratory of Oncogenes and Related Genes Shanghai Jiao Tong University160 Pujian Road200127 ShanghaiChina Department of Biliary-Pancreatic Surgery Renji HospitalSchool of MedicineShanghai Jiao Tong University160 Pujian Road200127 ShanghaiChina Department of General Surgery Sir Run Run Shaw HospitalThe Medicine School of Zhejiang UniversityEast Qinchun Road310020 HangzhouZhejiang ProvinceChina

Dear Editor,Gallbladder cancer(GBC),the most common malignant tumor of the biliary tract,is a highly invasive form of *** resection is currently the first line approach to effectively treat GBC;however,very few patients have the opportunity to receive radical surgical treatment due to lack of obvious symptoms.1 The median survival of patients with GBC is only 12 months and 5-year survival rate is<5%,indicating GBC is extremely poor ***,it is urgent to identify novel key molecules that can potentially serve as early diagnostic biomarkers and/or therapeutic *** current study focused on the potential role of bone morphogenetic protein 2 inducible kinase(BMP2K),a serine/threonine kinase,which was recently identified as clathrin-coated vesicle-associated protein in the development of GBC;the outcome may hold diagnostic and therapeutic promising for clinical practice.

关键词： cancer BMP2 gallbladder

Evodiamine induces apotosis and inhibits self-renewal of gastric cancer stem cells through the Wnt/β-catenin signaling pathway

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Evodiamine induces apotosis and inhibits self-renewal of gas...

2014年浙江省外科学学术年会

作者： Qiang Wang Zhimin Wang defei hong Department of hepatobiliary and Pancreatic Surgery Zhejiang Provincial People’s Hospital

Evodiamine derived from the traditional herbal medicine Evodia rutaecarpa,was reported various biological effects,such as antitumor,antinociceptive,and vasorelaxant ***,the effect of evodiamine on cancer stem cells(CSCs)remains *** this study,we demonstrated the inhibitory effects of evodiamine on gastric cancer stem cells(GCSCs)and the potential *** enriched GCSCs using serum-free medium,and cultured them in spheroid *** assays showed that the proliferation of GCSCs was inhibited by evodiamine at a dose-and time-dependent *** accordance with it,the percentage of Annexin V-positive GCSCs was effectively increased and the Bax/Bcl-2 ratio was upregulated by evodiamine through flow cytometric analysis and Western *** also decreased the sphere formation ability,the expression of Sox2,KLF4,Bmi-1and 0ct4,and the oxaliplatin resistance of gastric cancer *** also found that after evo treatment,there was an inhibitory effect on epithelial to mesenchymal transition(EMT),with the decreased expression of Slug,Twist,Zeb1,and ***,evodiamine treatment of spheroids from gastric cancer cells(GCCs)decreased the expression ofβ-catenin,c-Myc and Cyclin D1 by Western blot,which confirmed its inhibitory self-renewal of GCSCs through the Wnt/β-catenin signaling *** summary,evodiamine inhibits the Wnt/β-catenin signaling pathway to induce apoptosis of GCSCs,inhibit stem cells properties and repress EMT,which provides a prospect for CSCs-targeted therapy in gastric cancer.

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Evodiamine induces apotosis and inhibits self-renewal of gastric cancer stem cells through the Wnt/β-catenin signaling pathway

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Evodiamine induces apotosis and inhibits self-renewal of gas...

2014年微创外科学术年会

作者： Qiang Wang Zhimin Wang defei hong Department of hepatobiliary and Pancreatic Surgery Zhejiang Provincial People’s Hospital

Evodiamine derived from the traditional herbal medicine Evodia rutaecarpa,was reported various biological effects,such as antitumor,antinociceptive,and vasorelaxant ***,the effect of evodiamine on cancer stem cells(CSCs)remains *** this study,we demonstrated the inhibitory effects of evodiamine on gastric cancer stem cells(GCSCs)and the potential *** enriched GCSCs using serum-free medium,and cultured them in spheroid *** assays showed that the proliferation of GCSCs was inhibited by evodiamine at a dose-and time-dependent *** accordance with it,the percentage of Annexin V-positive GCSCs was effectively increased and the Bax/Bcl-2 ratio was upregulated by evodiamine through flow cytometric analysis and Western *** also decreased the sphere formation ability,the expression of Sox2,KLF4,Bmi-1and Oct4,and the oxaliplatin resistance of gastric cancer *** also found that after evo treatment,there was an inhibitory effect on epithelial to mesenchymal transition(EMT),with the decreased expression of Slug,Twist,Zeb1,and ***,evodiamine treatment of spheroids from gastric cancer cells(GCCs)decreased the expression ofβ-catenin,c-Myc and Cyclin D1 by Western blot,which confirmed its inhibitory self-renewal of GCSCs through the Wnt/β-catenin signaling *** summary,evodiamine inhibits the Wnt/β-catenin signaling pathway to induce apoptosis of GCSCs,inhibit stem cells properties and repress EMT,which provides a prospect for CSCs-targeted therapy in gastric cancer.

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胆囊癌诊断和治疗指南(2019版)

中华外科杂志 2020年第4期58卷 243-246,247-251页

作者：无全志伟洪德飞中华医学会外科学分会胆道外科学组中国医师协会外科医师分会胆道外科专业委员会上海交通大学医学院附属新华医院普外科 200092 浙江大学医学院附属邵逸夫医院普外科杭州310016

为提高我国胆囊癌总体治疗效果,中华医学会外科学分会胆道外科学组、中国医师协会胆道外科专业委员会制订了《胆囊癌诊断和治疗指南(2019版)》。该指南明确了胆囊癌发病的危险因素及干预方法、随访流程,描述了胆囊癌TNM最新分期,并提出... 详细信息

为提高我国胆囊癌总体治疗效果,中华医学会外科学分会胆道外科学组、中国医师协会胆道外科专业委员会制订了《胆囊癌诊断和治疗指南(2019版)》。该指南明确了胆囊癌发病的危险因素及干预方法、随访流程,描述了胆囊癌TNM最新分期,并提出了胆囊癌的临床分型。基于现代高质量影像技术和内镜技术可精准评估胆囊癌分期和分型,对进展期胆囊癌应贯彻综合治疗的理念,进行多学科讨论,科学决策治疗手段。胆囊癌的外科治疗应在具有丰富经验的肝胆胰外科医师和病理科医师的医疗中心完成,合理选择手术技术平台,实施规范的胆囊癌根治性切除术;化疗、放疗、靶向治疗及免疫治疗的结合应用可改善胆囊癌患者的预后,但仍需进一步研究使胆囊癌患者获得更大生存获益。

关键词：胆囊肿瘤危险因素诊断治疗

微创胰十二指肠切除术：从腹腔镜到达芬奇机器人手术系统

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中华消化外科杂志 2015年第11期14卷 980-982页

作者：张宇华洪德飞浙江省人民医院肝胆胰外科及微创外科杭州310014

自1994年第l例腹腔镜胰十二指肠切除术至2003年首次报道达芬奇机器人手术系统辅助胰十二指肠切除术的开展，微创胰十二指肠切除术经过20年的探索、发展，在争议和疑惑中取得了突破性的进展。微创胰十二指肠切除术在国内外一些大的医疗... 详细信息

自1994年第l例腹腔镜胰十二指肠切除术至2003年首次报道达芬奇机器人手术系统辅助胰十二指肠切除术的开展，微创胰十二指肠切除术经过20年的探索、发展，在争议和疑惑中取得了突破性的进展。微创胰十二指肠切除术在国内外一些大的医疗中心已成为治疗壶腹部周围肿瘤的常规治疗手术方式，并出现单中心100例以上的报道，以及与开腹胰十二指肠切除术大宗病例的近、远期疗效的对比研究报道。研究者及时更新腹腔镜和达芬奇机器人手术系统行胰十二指肠切除术的理念，总结经验和进展，有助于其普及和推广，从而造福患者。

关键词：胰腺疾病达芬奇机器人手术系统胰十二指肠切除术腹腔镜检查

Who took it away and who should return the due surgical training to the Chinese Surgical residents?

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Who took it away and who should return the due surgical trai...

2015年浙江省外科学学术年会暨国家级肝胆胰疾病诊治进展学习班

作者： Wang Zhifei Gao Yue Zhang Dahong hong defei Wang hong Huang Dongsheng Department of Hepatobiliary Pancreatic and Minimally Invasive SurgeryZhejiang Provincial People’s Hospital Biomedical Engineering Cleveland Clinic Foundation Department of Urology Zhejiang Provincial People’s Hospital Department of Anesthesiology.Wayne State University/Detroit Medical Center.McLaren Flint Hospital Department of General Surgery Sir RunRun Shaw HospitalSchool of MedicineZhejiang University

The Chinese government is making a forceful strike on the modernization of its healthcare *** education and training,as part of it,is undergoing tremendous *** the leading actions is the Standardized Resident Training ***,despite the huge clinical volume nationwide,the actual volume of hand-on clinical training is far lagged behind expectations,especially for surgical *** training for resident

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腹腔镜脾切除联合贲门周围血管离断术理念与技术创新

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中华腔镜外科杂志（电子版） 2018年第2期11卷 68-71页

作者：洪德飞浙江大学医学院附属邵逸夫医院普通外科杭州310016

自20世纪80年代我国著名外科学家裘法祖院士首创脾切除联合贲门周围血管离断术治疗肝硬化门静脉高压症以来,长期的临床实践证明SPD不仅有效控制上消化道出血,同时又能维持肝血流灌注,改善肝功能,提高免疫力。

关键词：贲门周围血管离断术腹腔镜脾切除技术创新肝硬化门静脉高压症上消化道出血肝血流灌注临床实践裘法祖

Advancing robotic surgery skills with a simple novel model for robotic training

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Advancing robotic surgery skills with a simple novel model f...

2015年浙江省外科学学术年会暨国家级肝胆胰疾病诊治进展学习班

作者： Wang Zhifei Liu Junwei Zhang Yuhua hong defei Yu Xiaofen Zhang Dahong Anusak Yiengpruksawan Huang Dongsheng Department of Hepatobiliary and Pancreatic Surgery Zhejiang People's Provincial Hospital

Background Both dry lab and wet lab training are importance for advanced surgery *** assisted surgery,though lowering the technical demand for some key surgery skills such as suturing,tying knots compared with traditional laparoscopic surgery,to master the techniques for the key surgery procedures is still an challenging *** challenge lies in several issues at the beginning for robotic *** coordination with

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