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Single‑cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes

Journal of Animal Science and Biotechnology 2024年第2期15卷 614-631页

作者： Ting Ge Yifan Wen bo Li Xiaoyu Huang Shaohua jiang Enping Zhang Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China

Background In the modern sheep production systems,the reproductive performance of ewes determines the economic profitability of *** the genetic mechanisms underlying differences in the litter size is important for the selection and breeding of highly prolific *** sheep,a high-quality Chinese sheep breed,is known for its high fecundity and is often used as a model to study prolificacy *** the current study,animals were divided into two groups according to their delivery rates in three consecutive lambing seasons(namely,the high and low reproductive groups with≥3 lambs and one lamb per season,n=3,respectively).The ewes were slaughtered within 12 h of estrus,and unilateral ovarian tissues were collected and analyzed by 10×Genomics single-cell RNA *** A total of 5 types of somatic cells were identified and corresponding expression profiles were mapped in the ovaries of each ***,the differences in the ovary somatic cell expression profiles between the high and low reproductive groups were mainly clustered in the granulosa ***,four granulosa cell subtypes were *** analysis revealed that the abundance of JPH1 expression and the reduction of LOC101112291 expression could lead to different evolutionary directions of the granulosa ***,the expression levels of FTH1 and FTL in mural granulosa cells of the highly reproductive group were significantly *** genes inhibit necroptosis and ferroptosis of mural granulosa cells,which helps prevent follicular *** This study provides insights into the molecular mechanisms underlying the high fecundity of Hu *** differences in gene expression profiles,particularly in the granulosa cells,suggest that these cells play a critical role in female *** findings also highlight the importance of genes such as JPH1,LOC101112291,FTH1,and FTL in regulating granulosa cell function and follicular development.

关键词： Granulosa cells Hu sheep Lambing number Ovarian somatic cells Single-cell RNA sequencing

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Inference analysis of relationships between best linear minimum bias predictors under two transformed general linear models

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Acta Mathematica Sinica,English Series 2024年

作者： bo jiang Yongge TIAN College of Mathematics and Information Science and Yantai Key Laboratory of Big Data Modeling and Intelligent Computing Shandong Technology and Business University College of Business and Economics Shanghai Business School

Regression models are often transformed into certain alternative forms in statistical inference theory. In this paper, we assume that a general linear model(GLM) is transformed into two different forms, and our aim is to study some comparison problems under the two transformed general linear models(TGLMs). We first construct a general vector composed of all unknown parameters under the two different TGLMs, derive exact expressions of best linear minimum bias predictors(BLMBPs)by solving a constrained quadratic matrix-valued function optimization problem in the L¨owner partial ordering, and describe a variety of mathematical and statistical properties and performances of the BLMBPs. We then approach some algebraic characterization problems concerning relationships between the BLMBPs under two different TGLMs. As applications, two specific cases are presented to illustrate the main main contributions in the study.

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Visceral adipose tissue predicts severity and prognosis of acute pancreatitis in obese patients

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Hepatobiliary & Pancreatic Diseases International 2024年第5期23卷 458-462页

作者： Yuan Tian Qing Huang Yu-Tang Ren Xuan jiang bo jiang Department of Gastroenterology Beijing Tsinghua Changgung HospitalSchool of Clinical MedicineTsinghua UniversityBeijing 102218China

Acute pancreatitis is a common systemic inflammatory disease, manifested by a spectrum of severity, ranging from mild in the majority of patients to severe acute pancreatitis. Patients with severe acute pancreatitis suffer from severe local and systemic complications and organ failure, leading to a poor prognosis. The early recognition of the severe condition is important to improve prognosis. Obesity has risen in tandem with an increase in the severity of acute pancreatitis in recent years. Studies have revealed that adipose tissue, particularly visceral adipose tissue is associated with the prognosis of acute pancreatitis. This review discussed the role of visceral adipose tissue in obese patients with acute pancreatitis and explored the possible mechanism involved.

关键词： Visceral adipose tissue Obesity Acute pancreatitis

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A novel alternative for pyrogen detection based on a transgenic cell line

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Signal Transduction and Targeted Therapy 2024年第3期9卷 1221-1229页

作者： Qing He Chuan-Fei Yu Gang Wu Kai-Qin Wang Yong-bo Ni Xiao Guo Zhi-Hao Fu Lan Wang De-jiang Tan Hua Gao Can Wang Gang Chen Xu-hong Chen bo Chen Jun-Zhi Wang National Institutes for Food and Drug Control BeijingChina School of Life Science and Biopharmaceutics Shenyang Pharmaceutical UniversityShenyangChina Shanghai Institute for Food and Drug Control ShanghaiChina KeyMed Biosciences Inc. ChengduChina

Pyrogen,often as a contaminant,is a key indicator affecting the safety of almost all parenteral drugs(including biologicals,chemicals,traditional Chinese medicines and medical devices).It has become a goal to completely replace the in vivo rabbit pyrogen test by using the in vitro pyrogen test based on the promoted‘reduction,replacement and refinement’principle,which has been highly considered by regulatory agencies from different *** used NF-κB,a central signalling molecule mediating inflammatory responses,as a pyrogenic marker and the monocyte line THP-1 transfected with a luciferase reporter gene regulated by NF-κB as an in vitro model to detect pyrogens by measuring the intensity of a fluorescence ***,we show that this test can quantitatively and sensitively detect endotoxin(lipopolysaccharide from different strains)and nonendotoxin(lipoteichoic acid,zymosan,peptidoglycan,lectin and glucan),has good stability in terms of NF-κB activity and cell phenotypes at 39 cell passages and can be applied to detect pyrogens in biologicals(group A&C meningococcal polysaccharide vaccine;basiliximab;rabies vaccine(Vero cells)for human use,freeze-dried;Japanese encephalitis vaccine(Vero cells),inactivated;insulin aspart injection;human albumin;recombinant human erythropoietin injection(CHO Cell)).The within-laboratory reproducibility of the test in three independent laboratories was 85%,80%and 80%and the interlaboratory reproducibility among laboratories was 83.3%,95.6%and 86.7%.The sensitivity(true positive rate)and specificity(true negative rate)of the test were 89.9%and 90.9%,*** summary,the test provides a novel alternative for pyrogen detection.

关键词： pyrogen rabbit replace

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Graph-decomposed k-NN searching algorithm on road network

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Frontiers of Computer Science 2024年第3期18卷 243-245页

作者： Wei jiang bo NING Guanyu LI Mei BAI Xiao JIA Fangliang WEI Faculty of Information Science and Technology Dalian Maritime UniversityDalian 116026China jiangxing Intelligence Inc. Shenzhen 518100China

1 Introduction The k-NN(k Nearest Neighbors)searching problem on road network constitutes a crucial aspect of location-oriented services,which has boasted the practical implications.

关键词： services searching network

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Emerging role of vitamin D_(3)in alleviating intestinal structure injury caused by Aeromonas hydrophila in grass carp(Ctenopharyngodon idella)

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Animal Nutrition 2024年第1期16卷 202-217页

作者： Yao Zhang Xiao-Qiu Zhou Wei-Dan jiang Pei Wu Yang Liu hong-Mei Ren Lu Zhang Hai-Feng Mi Ling Tang Cheng-bo Zhong Lin Feng Animal Nutrition Institute Sichuan Agricultural UniversityChengduChina Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengduChina Key Laboratory for Animal Disease-Resistant Nutrition of China Ministry of Education Sichuan Agricultural UniversityChengduChina Healthy Aquaculture Key Laboratory of Sichuan Province Tongwei Co.Ltd.ChengduChina Sichuan Animal Science Academy Sichuan Animtech Feed Co.Ltd.ChengduChina

Bacterial pathogens destroy the structural integrity of functional organs in fish,leading to severe challenges in the aquaculture *** D_(3)(VD_(3))prevents bacterial infections and strengthens immune system function via vitamin D receptor(VDR).However,the correlation between VD_(3)/VDR and the structural integrity of functional organs remains *** study aimed to investigate the influence of VD_(3)supplementation on histological characteristics,apoptosis,and tight junction characteristics in fish intestine during pathogen infection.A total of 540 healthy grass carp(257.24±0.63 g)were fed different levels of VD_(3)(15.2,364.3,782.5,1,167.9,1,573.8,and 1,980.1 IU/kg)for 70 ***,fish were challenged with Aeromonas hydrophila,a pathogen that causes intestinal *** present study demonstrated that optimal supplementation with VD_(3)(1)alleviated intestinal structural damage,and inhibited oxidative damage by reducing levels of oxidative stress biomarkers;(2)attenuated excessive apoptosisrelated death receptor and mitochondrial pathway processes in relation to p38 mitogen-activated protein kinase signaling(P<0.05);(3)enhanced tight junction protein expression by inhibiting myosin light chain kinase signaling(P<0.05);and(4)elevated VDR isoform expression in fish intestine(P<0.05).Overall,the results demonstrated that VD_(3)alleviates oxidative injury,apoptosis,and the destruction of tight junction protein under pathogenic infection,thereby strengthening pathogen defenses in the *** finding supports the rationale for VD_(3)intervention as an essential practice in sustainable aquaculture.

关键词： Aeromonas hydrophila Antibacterial property Grass carp(Ctenopharyngodon idella) Intestinal health Vitamin D_(3)

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Identification of clinical subphenotypes of sepsis after laparoscopic surgery

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Laparoscopic, Endoscopic and Robotic Surgery 2024年第1期7卷 16-26页

作者： Jie Yang bo Zhang Chaomin Hu Xiaocong jiang Pengfei Shui Jiajie Huang Yucai hong hongying Ni Zhongheng Zhang Department of Emergency Medicine Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Department of Emergency Medicine Huzhou Central HospitalHuzhou 313000China Department of Critical Care Medicine Affiliated Jinhua HospitalZhejiang University School of MedicineJinhua 321000China

Objective:Some patients exhibit septic symptoms following laparoscopic surgery,leading to a poor *** clinical subphenotyping is critical for guiding tailored therapeutic strategies in these *** identifying predisposing factors for postoperative sepsis,clinicians can implement targeted interventions,potentially improving *** study outlines a workflow for the subphenotype methodology in the context of laparoscopic surgery,along with its practical ***:This study utilized data routinely available in clinical case systems,enhancing the applicability of our *** data included vital signs,such as respiratory rate,and laboratory measures,such as blood sodium *** process of categorizing clinical routine data involved technical complexities.A correlation heatmap was used to visually depict the relationships between *** points were used to identify the clustering structure and combined with Consensus K clustering methods to determine the optimal ***:Our study highlighted the intricacies of identifying clinical subphenotypes following laparoscopic surgery,and could thus serve as a valuable resource for clinicians and researchers seeking to explore disease heterogeneity in clinical *** simplifying complex methodologies,we aimed to bridge the gap between technical expertise and clinical application,fostering an environment where professional medical knowledge is effectively utilized in subphenotyping ***:This tutorial could primarily serve as a guide for beginners.A variety of clustering approaches were explored,and each step in the process contributed to a comprehensive understanding of clinical subphenotypes.

关键词： Laparoscopic surgery Phenotype Precision medicine Sepsis

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Bibliometrics analysis based on the Web of Science:Current trends and perspective of gastric organoid during 2010-2023

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World Journal of Gastroenterology 2024年第8期30卷 969-983页

作者： Kai-Lin jiang Yue-bo Jia Xue-Jiao Liu Qing-Ling Jia Li-Kun Guo Xiang-Xiang Wang Ke-Ming Yang Chen-Heng Wu Bei-Bei Liang jiang-hong Ling Department of Gastroenterology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghai 200021China Laboratory of Cancer Biology University of OxfordOxford OX37DQUnited Kingdom School of Traditional Chinese Medicine Shanghai University of Chinese MedicineShanghai 200021China Shanghai Key Laboratory of Molecular Imaging Shanghai University of Medicine and Health SciencesShanghai 201318China

BACKGROUND Three-dimensional organoid culture systems have been established as a robust tool for elucidating mechanisms and performing drug efficacy *** use of gastric organoid models holds significant promise for advancing personalized medicine ***,a comprehensive bibliometric review of this burgeoning field has not yet been *** To analyze and understand the development,impact,and direction of gastric organoid research using bibliometric methods using data from the Web of Science Core Collection(WoSCC)*** This analysis encompassed literature pertaining to gastric organoids published between 2010 and 2023,as indexed in the *** and VOSviewer were used to depict network maps illustrating collaborations among authors,institutions and keywords related to gastric ***,co-citation,and burst analysis methodologies were applied to assess the impact and progress of *** A total of 656 relevant studies were *** majority of research was published in gastroenterology-focused ***,Yana Zavros,Hans Clevers,James M Wells,Sina Bartfeld,and Chen Zheng were the 5 most productive authors,while Hans Clevers,Huch Meritxell,Johan H van Es,Marc Van de Wetering,and Sato Toshiro were the foremost influential scientists in this *** from the University Medical Center Utrecht,Netherlands Institute for Developmental Biology(Utrecht),and University of Cincinnati(Cincinnati,OH,United States)made the most significant ***,gastric organoids are used mainly in studies investigating gastric cancer(GC),Helicobacter pylori-infective gastritis,with a focus on the mechanisms of GC,and drug screening *** Key focus areas of research using gastric organoids include unraveling disease mechanisms and enhancing drug screening *** contributions from renowned academic institutions highlight this field’s dynamic growth.

关键词： Gastric organoid Gastric disease Bibliometrics analysis Gastric cancer Gastritis

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Single-atom Ni-N_(4)for enhanced electrochemical sensing

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Nano Research 2024年第8期17卷 7658-7664页

作者： Zhuhui Qin bo Tang Guiru Zhang Chongqing Zhu Kun jiang bowei Zhang Fu-Zhen Xuan Shanghai Key Laboratory of Intelligent Sensing and Detection Technology East China University of Science and TechnologyShanghai 200237China Key Laboratory of Pressure Systems and Safety of Ministry of Education East China University of Science and TechnologyShanghai 200237China School of Mechanical and Power Engineering East China University of Science and TechnologyShanghai 200237China Key Laboratory of Theoretical and Computational Photochemistry Ministry of EducationCollege of ChemistryBeijing Normal UniversityBeijing 100875China Interdisciplinary Research Center School of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240China

Single-atom catalysts(SACs)attract widespread attention in heterogeneous catalysis due to their maximum atomic utilization efficiency and unique physical and chemical ***,their applications in chemical sensing keep huge potential but remain ***,a Ni-N_(4)-C SAC was synthesized for the trace detection of dopamine(DA)and uric acid(UA).The Ni-N_(4)-C SAC exhibited superior sensing performance compared to the Ni *** detection range for DA and UA were 0.05–75μM and 5–90μM with detection limits of 0.027 and 0.82μM,*** functional theory(DFT)computations indicate that Ni-N_(4)-C has a lower reaction barrier during electrochemical process,indicating that the atomic Ni sites possess higher intrinsic activity than Ni ***,DA and UA show strong potential dependency on the Ni-N_(4)-C catalyst,indicating its applicability for their concurrent *** work extends the application of SACs in chemical sensing.

关键词： single-atom catalysts chemical sensing dopamine uric acid

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Coherent link between a Ti:sapphire comb and a 1.5μm laser via nonlinear interaction in photonic crystal fiber

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Photonics Research 2024年第2期12卷 350-355页

作者： YUAN YAO HAOSEN SHI GUANG YANG bo LI CONGYU WANG hongFU YU LONGSHENG MA YANYI jiang State Key Laboratory of Precision Spectroscopy East China Normal UniversityShanghai 200062China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China

Optical clock networks have distinct advantages for the dissemination of time/frequency,geodesy,and fundamental *** realize such a network,the telecom band and optical atomic clocks have to be coherently *** the telecom band and optical atomic clocks reside in a distinct spectral region,second-harmonic generation is usually introduced to bridge the large frequency *** this paper,we introduce a new method to coherently link a 1550 nm continuous wave laser with a Ti:sapphire mode-locked laser-based optical frequency *** coupling the 1550 nm continuous wave laser light and the Ti:sapphire comb light together into a photonic crystal fiber,nonlinear interaction takes place,and new comblike frequency components related to the 1550 nm laser frequency are generated in the visible ***,we can detect beat notes between two combs in the visible region with a signal-to-noise ratio of more than 40 dB in a resolution bandwidth of300 *** this signal,we realize an optical frequency divider for converting the frequency of optical clocks in the visible region to the telecom band at 1.55μ*** out-of-loop measurement shows that the additional noise and uncertainty induced in optical frequency conversion are 5×10^(-18)at 1 s averaging time and 2.2×10^(-19),respectively,which are limited by the uncompensated light path fluctuation but fulfill precision measurement using state-of-the-art optical clocks.

关键词： fiber interaction nonlinear

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