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miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow-derived neural crest cells

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Neural Regeneration Research 2025年第1期20卷 277-290页

作者： Meng Cong Jing-Jing Hu yan.Yu Xiao-Li Li Xiao-Ting Sun Li-Ting Wang Xia Wu Ling-Jie Zhu Xiao-Jia yan. qian-Ru He Fei Ding Hai-yan.Shi School of Medicine Nantong UniversityNantongJiangsu ProvinceChina Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education and Coinnovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina Department of Physiology Jiangsu Health Vocational CollegeNanjingJiangsu ProvinceChina Department of Pathology Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina Jiangsu Clinical Medicine Center of Tissue Engineering and Nerve Injury Repair Affiliated Hospital of Nantong UniversityNantongJiangsu ProvinceChina

Our previous study found that rat bone marrow–derived neural crest cells(acting as Schwann cell progenitors)have the potential to promote long-distance nerve ***-based therapy can enhance peripheral nerve repair and regeneration through paracrine bioactive factors and intercellular ***,the complex contributions of various types of soluble cytokines and extracellular vesicle cargos to the secretome remain *** investigate the role of the secretome and extracellular vesicles in repairing damaged peripheral nerves,we collected conditioned culture medium from hypoxia-pretreated neural crest cells,and found that it significantly promoted the repair of sensory neurons damaged by oxygen-glucose *** mRNA expression of trophic factors was highly expressed in hypoxia-pretreated neural crest *** performed RNA sequencing and bioinformatics analysis and found that miR-21-5p was enriched in hypoxia-pretreated extracellular vesicles of neural crest ***,to further clarify the role of hypoxia-pretreated neural crest cell extracellular vesicles rich in miR-21-5p in axonal growth and regeneration of sensory neurons,we used a microfluidic axonal dissociation model of sensory neurons in vitro,and found that hypoxia-pretreated neural crest cell extracellular vesicles promoted axonal growth and regeneration of sensory neurons,which was greatly dependent on loaded ***,we constructed a miR-21-5p-loaded neural conduit to repair the sciatic nerve defect in rats and found that the motor and sensory functions of injured rat hind limb,as well as muscle tissue morphology of the hind limbs,were obviously *** findings suggest that hypoxia-pretreated neural crest extracellular vesicles are natural nanoparticles rich in ***-21-5p is one of the main contributors to promoting nerve regeneration by the neural crest cell *** helps to explain the mechanism of action of the secretome and e

关键词： axotomy cell-free therapy conditioned medium extracellular vesicles hypoxic preconditioning microRNA oxygen-glucose deprivation peripheral nerve injury Schwann cell precursors

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调整细胞培养条件提高间充质干细胞外泌体治疗骨关节炎的潜能

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中国组织工程研究 2025年第1期29卷 164-174页

作者：钱燕刘启颂深圳国家感染性疾病临床医学研究中心南方科技大学深圳市第三人民医院广东省深圳市518112

背景:间充质干细胞外泌体有望发展成为治疗骨关节炎的无细胞疗法,而通过调整细胞培养条件,可以进一步提高其治疗活性和临床转化潜能。目的:综述分析细胞培养条件对外泌体活性的影响,分析其临床转化潜力。方法:查阅国内外有关骨关节炎/... 详细信息

背景:间充质干细胞外泌体有望发展成为治疗骨关节炎的无细胞疗法,而通过调整细胞培养条件,可以进一步提高其治疗活性和临床转化潜能。目的:综述分析细胞培养条件对外泌体活性的影响,分析其临床转化潜力。方法:查阅国内外有关骨关节炎/软骨损伤修复与间充质干细胞来源外泌体的文献。检索词分别为“外泌体,间充质干细胞,骨关节炎,软骨修复”和“exosomes,extracellular vesicles,mesenchymal stem cells,osteoarthritis,cartilage repair”,检索数据库分别为PubMed和中国知网数据库,检索时限为2010-2023年,最后纳入100篇文献进行总结和分析。结果与结论:(1)在间充质干细胞的培养过程中,提供有利于软骨细胞生长的物理环境,或添加软骨保护小分子、成软骨诱导因子和炎症因子等刺激细胞,可以进一步提高间充质干细胞外泌体在维持软骨细胞表型、促进软骨再生和免疫抑制等方面的调控活性;因此,其他具有相似特征的物理或化学因素,也有可能提升间充质干细胞外泌体治疗骨关节炎的疗效。(2)低氧诱导、脉冲电磁场刺激、生物反应器及软骨保护分子(如人甲状旁腺素1-34)刺激等细胞培养方案具有安全、可规模化生产等优点,可用于制备临床用途的、疗效好的间充质干细胞外泌体。(3)间充质干细胞外泌体有望实现骨关节炎的修正治疗,通过调整细胞培养方案提升治疗活性,可进一步提高其临床转化的可行性。

关键词：间充质干细胞外泌体骨关节炎软骨修复细胞培养低氧诱导预处理三维培养软骨保护因子炎症因子

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Mediating role of health literacy in the relationship between social isolation and psychological distress among pre-frail older adults

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World Journal of Psychiatry 2025年第1期15卷 137-144页

作者： Juan Fang Li-Hui Li Ming-qian He Yue Ji Dong-yan.Lu Li-Bing Zhang Jin-Lan Yao School of Medicine Huzhou UniversityHuzhou 313000Zhejiang ProvinceChina

BACKGROUND Frailty has become a significant public health *** recent increase in the number of frail older adults has led to increased attention being paid to psycho-logical care services in *** social isolation of pre-frail older adults can impact their psychological *** To explore the mediating effect of health literacy between social isolation and psychological distress among communitydwelling older adults with *** This descriptive cross-sectional study was conducted with 254 pre-frail older individuals aged 60 years and *** isolation,health literacy,and psycholo-gical distress were comprehensively measured using the Lubben Social Network Scale-6,12-item Short-Form Health Literacy Questionnaire,and the Kessler Psy-chological Distress *** were evaluated using the SPSS 27.0 package program and the PROCESS macro *** statistical analyses,correlation analyses,and bootstrap mediation tests were used to assess associations between the *** The results showed that social isolation had an effect on health literacy among pre-frail older adults(β=0.240,P<0.001),social isolation impact on psycho-logical distress pre-frail older adults(β=-0.415,P<0.001);health literacy was identified effect on psychological distress among pre-frail older persons(β=-0.307,P<0.001).Health literacy partially mediated the relationship between social isolation and psychological distress among community-dwelling older adults with pre-frailty,with a mediation effect of-0.074,accounting for 17.83%of the total effect.

关键词： Frail Ageing Psychological distress Health literacy Social isolation

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A Bilinear Backlund Transformation and Lax Pair for a （1＋1）-Dimensional Differential-Difference sine-Gordon Equation

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Communications in Theoretical Physics 2007年第3X期48卷 487-490页

作者： qian Xian-Min hon-Wah Tam Department of Physics Shaoxing College of Arts and Sciences Shaoxing 312000 China Department of Physics Shanghai Jiao Tong University Shanghai 200030 China Department of Computer Science Hong Kong Baptist University Hong Kong China

In this paper, we obtain a 1＋1 dimensional integrable differential-difference model for the sine-Gordon equation by Hirota＇s discretization method. A bilinear Backlund transformation and the associated Lax pair are ... 详细信息

关键词： sine-Gordon equation Hirota's discretization method Backlund transformation Lax pair

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糖尿病肾病模型:动物模型、二维细胞模拟及三维类器官模型

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中国组织工程研究 2025年第17期29卷 3632-3640页

作者：钱足平陈勇冉燕达静静查艳遵义医科大学贵州省遵义市563000 贵州省人民医院肾内科贵州省贵阳市550002

背景:近几年来,人类多能干细胞衍生的肾类器官及糖尿病肾病啮齿动物模型取得了一定的进展。然而,由于糖尿病肾病发病受环境因素及遗传因素共同作用,发病机制复杂,并且对于此类患者的临床治疗方法需要因人而异。因此,需要开发更灵活和综... 详细信息

背景:近几年来,人类多能干细胞衍生的肾类器官及糖尿病肾病啮齿动物模型取得了一定的进展。然而,由于糖尿病肾病发病受环境因素及遗传因素共同作用,发病机制复杂,并且对于此类患者的临床治疗方法需要因人而异。因此,需要开发更灵活和综合的方法,从而发现强有力的临床前证据,以支持对糖尿病肾病患者进行更有针对性的干预。目的:从动物模型、二维细胞培养模拟及三维类器官模型等方面综述了糖尿病肾病模型的研究进展,为进一步的研究提供线索和思路。方法:以“糖尿病,糖尿病肾病,糖尿病肾病模型,糖尿病肾病动物模型,类器官,肾脏类器官,糖尿病肾病细胞模型,糖尿病肾病病证结合动物模型”为中文检索词,“Diabetes,diabetic nephropathy,diabetic kidney diseases,diabetic nephropathy models,diabetic nephropathy animal models,organoids,diabetic and organoids,diabetic and kidney organoids,kidney organoids,diabetic nephropathy cell models,diabetic nephropathy syndrome combined animal models”为英文检索词,检索中国知网及PubMed数据库,最终纳入101篇文献进行综述分析。结果与结论:糖尿病肾病体内模型和体外模型是深入研究糖尿病肾病发病机制的有力工具。动物模型可以观察到多系统之间的相互作用;二维细胞培养操作简便且成本较低;新兴的肾类器官填补了二维和整体水平之间的空白,无种属差异且一定程度模拟了人肾脏的复杂性。随着类器官技术的不断发展,肾类器官有望为探索糖尿病肾病的发病机制、病理生理过程和药物筛选提供一个新的视角。

关键词：糖尿病肾病肾脏类器官糖尿病肾病类器官细胞模型动物模型中医病证结合糖尿病肾病模型工程化组织构建

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Defined hydrogels for spinal cord organoids:challenges and potential applications

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Neural Regeneration Research 2024年第11期19卷 2329-2330页

作者： Wai hon Chooi Yuewen Wu Shi-yan.Ng Institute of Molecular and Cell Biology(IMCB) Agency for ScienceTechnology and Research(A*STAR)SingaporeSingapore Department of Physiology Yong Loo Lin School of MedicineNational University of Singapore(NUS)SingaporeSingapore

Organoids of the central nervous system,primarily derived from pluripotent stem cells or neural stem cells,are three-dimensional tissue cultures with self-organizing *** exposed to the right combinations of signals,they differentiate into a 3D tissue consisting of complex cytoarchitecture and native cell types,including various neuron subtypes and glial *** features closely mimic native tissues,making them invaluable for developmental studies and disease *** recent years,spinal cord organoids(SCOs)have been developed to investigate spinal cord development,injuries,and various neurological *** an integral part of the central nervous system,SCOs play a vital role and serve as a site for studying both neurodevelopment and neurodegenerative diseases.

关键词： properties injuries primarily

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The role of natural flavonoids on neuroinflammation as a therapeutic target for Alzheimer's disease:a narrative review

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Neural Regeneration Research 2023年第12期18卷 2582-2591页

作者： qian Zhang Yaping yan National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China Key Laboratory of Medicinal Resources and Natural Pharmaceutical ChemistryThe Ministry of EducationCollege of Life SciencesShaanxi Normal UniversityXi’anShaanxi ProvinceChina

Alzheimer's disease is a neurodegenerative disease that affects a large proportion of older adult people and is characterized by memory loss,progressive cognitive impairment,and various behavioral *** the pathological mechanisms underlying Alzheimer's disease are complex and remain unclear,previous research has identified two widely accepted pathological characteristics:extracellular neuritic plaques containing amyloid beta peptide,and intracellular neurofibrillary tangles containing ***,research has revealed the significant role played by neuroinflammation over recent *** inflammatory microenvironment mainly consists of microglia,astrocytes,the complement system,chemokines,cytokines,and reactive oxygen intermediates;collectively,these factors can promote the pathological process and aggravate the severity of Alzheimer's ***,the development of new drugs that can target neuroinflammation will be a significant step forward for the treatment of Alzheimer's *** are plant-derived secondary metabolites that possess various *** research found that multiple natural flavonoids could exert satisfactory treatment effects on the neuroinflammation associated with Alzheimer's *** this review,we describe the pathogenesis and neuroinflammatory processes of Alzheimer's disease,and summarize the effects and mechanisms of 13 natural flavonoids(apigenin,luteolin,naringenin,quercetin,morin,kaempferol,fisetin,isoquercitrin,astragalin,rutin,icariin,mangiferin,and anthocyan.n)derived from plants or medicinal herbs on neuroinflammation in Alzheimer's *** an important resource for the development of novel compounds for the treatment of critical diseases,it is essential that we focus on the exploitation of natural *** particular,it is vital that we investigate the effects of flavonoids on the neuroinflammation associated with Alzheimer's disease in greater detail.

关键词： Alzheimer's disease astrocytes flavonoids microglia natural products neuroinflammation

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Pediatric COVID‑19:what disease is this?

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World Journal of Pediatrics 2020年第4期16卷 323-325页

作者： Kam Lun Ellis hon Karen Ka yan.Leung Department of Paediatrics and Adolescent Medicine The Hong Kong Children’s HospitalHong Kong SARChina

The coronavirus disease 2019(COVID-19)pandemic spares no nations or cities causing escalating incidence and ***,prime ministers,celebrities and high government officials alike have been affected by the *** peculiar reasons,children and infants have generally been spared in hong Kong,China until recently,when returning students from affected cities with the virus who largely presented with mild *** fact,several countries have reported on pediatric COVID-19.

关键词： mortality officials Royal

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Comparative study of performance comparison of AlSi10Mg alloy prepared by selective laser melting and casting

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Journal of Materials Science & Technology 2020年第6期41卷 199-208页

作者： qian yan.Bo Song Yusheng Shi State Key Laboratory of Materials Processing and Die&Mould Technology Huazhong University of Science and TechnologyWuhan430074China

The influence of the microstructure on mechanical properties of AlSi10Mg fabricated by casting and selective laser melting(SLM)were investigated and contrasted in this work,with an emphasis on understanding the forming *** microstructure,phase structure and mechanical properties were characterized by scanning electron microscopy/field-emission Transmission Electron Microscopy(SEM/TEM),X-Ray Diffraction(XRD),tensile and fatigue *** results indicated that the SLM AlSi10Mg exhibited a supersaturated Si network structure precipitated alongα-Al ***β-Al5FeSi andπ-Al8FeMg3Si6 phases were found in the as-cast and SLM AlSi10Mg respectively due to different thermal histories during *** SLM AlSi10Mg showed higher tensile strength but lower elongation than the casting,as the result of grain refinement and tortuous crack *** fatigue results revealed that unmelted powder,oxide inclusion and pores can considerably degrade the fatigue properties for the SLM *** SLM process offered a new method for material processing that would avoid harmful Fe-bearing intermetallic compounds and refine the microstructures for enhancing strength.

关键词： Selective laser melting Casting AlSi10Mg Intermetallic compound Mechanical properties

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RESEARCH ON IMPROVED CUT-OFF NEGATIVE PRESSURE METHOD FOR UNSTEADY SEEPAGE FLOW WITH FREE SURFACE

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Journal of Hydrodynamics 2005年第5期17卷 521-531页

作者： ZHANG qian-fei WANG yan.ming Shanghai Municipal Engineering Design Institute Shanghai 200092 China

The cut-off negative pressure method is one of effective approaches to solve the seepage flow with free surface. According to the characteristics of this method, however, it has been found out that the deficiency of the algorithms is to easily cause inaccurate simulation in the vicinity of free surface owing to the penalty function with only one controlling penalty parameter. Therefore, an improved cut-off pressure method was presented by constructing penalty function with two controlling penalty parameters. The improved algorithms were illustrated to be able to improve the simulation accuracy and raise the convergence speed through considering the effects of the partially saturated or unsaturated elements more reasonably and calculating the nodal external forces more accurately. Finally, the improved cut-off negative pressure method was applied to the simulation of unsteady seepage flow with free surface, and the corresponding program codes were developed with validation of some cases.

关键词： free surface unsteady seepage flow finite element method improved cut-off negative pressure method

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