检索结果-南通市图书馆

Effects of broccoli extract and various essential oils on intestinal and faecal microflora and on xenobiotic enzymes and the antioxidant system of piglets

引用

Open Journal of Animal Sciences 2012年第2期2卷 78-98页

作者： Kristin Mueller Nicole M. Blum holger kluge Rolf Bauerfeind Julia Froehlich Anneluise Mader Karola R. Wendler Andreas S. Mueller Delacon Biotechnik Ges.m.b.H. Steyregg Austria Institute of Agricultural and Nutritional Sciences Preventive Nutrition Group Martin Luther University of Halle-Wittenberg Halle Germany Institute of Animal Nutrition Free University of Berlin Berlin Germany Institute of Veterinary Hygiene and Infectious Diseases of Animals Justus Liebig University Giessen Giessen Germany

Objective: Since the ban of antibiotics as growth promoting feed additives in the EU in 2006 research in alternatives has gained importance. Phytogenic feed additives represent a heterogenous class of different plant derived substances that are discussed to improve the health of farm animals by direct and indirect antioxidant effects and by influencing microbial eubiosis in the gastrointestinal tract. Consequently our study aimed to investigate the influence of broccoli extract and the essential oils of tur- meric, oregano, thyme and rosemary, as selected individual additives, on intestinal and faecal microflora, on xenobiotic enzymes, and on the antioxidant system of piglets. Methods: 48 four weeks old male weaned piglets were assigned to 6 groups of 8. The piglets were housed individually in stainless steel pens with slatted floor. The control group (Con) was fed a diet without an additive for 4 weeks. The diet of group BE contained 0.15 g/kg sulforaphane in form of a broccoli extract. 535, 282, 373 and 476 mg/kg of the essential oils of turmeric (Cuo), oregano (Oo), thyme (To) and rosemary (Ro) were added to the diets of the remaining 4 groups to stan-dardise supplementation to 150 mg/kg of the oils’ key terpene compounds ar-turmerone, carvacrol, thymol and 1,8-cineole. The composition of bacterial microflora was examined by cultivating samples of jejeunal and colonic mucosa and of faeces under specific conditions. The mRNA expression of xenobiotic and antioxidant enzymes was determined by reversing transcrip- tase real time detection PCR (RT-PCR). Total antioxidant status was assayed using the Trolox Equivalent Antioxidant Capacity (TEAC), and lipid peroxidation was determined by measuring thiobarbioturic acid reactive substances (TBA- RS). Results: Compared to Con piglets all additives positively influenced weight gain and feed conversion in week 1. Over the whole trial period no significant differences in performance parameters existed between the experimental

关键词： Pigs Phytogenic Feed Additives Broccoli Extract Essential Oils Xenobiotic Enzymes Antioxidant System

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Regulation of gene-edited plants in Europe:from the valley of tears into the shining sun?

引用

aBIOTECH 2024年第2期5卷 231-238页

作者： holger Puchta Department of Molecular Biology Joseph Gottlieb Kolreuter Institute for Plant SciencesKarlsruhe Institute of Technology(KIT)Fritz-Haber-Weg 476131 KarlsruheGermany

Some 20 years ago,the EU introduced complex regulatory rules for the growth of transgenic crops,which resulted in a de facto ban to grow these plants in fields within most European *** the rise of novel genome editing technologies,it has become possible to improve crops genetically in a directed way without the need for incorporation of foreign ***,in 2018,the European Court of Justice ruled that such gene-edited plants are to be regulated like transgenic *** then,European scientists and breeders have challenged this decision and requested a revision of this outdated ***,after 5 years,the European Commission has now published a proposal on how,in the future,to regulate crops produced by new breeding *** proposal tries to find a balance between the different interest groups in *** one side,genetically modified plants,which cannot be discerned from their natural counterparts,will exclusively be used for food and feed and are-besides a registration step-not to be regulated at *** the other side,plants expressing herbicide resistance are to be excluded from this regulation,a concession to the strong environmental associations and NGOs in ***,edited crops are to be excluded from organic farming to protect the business interests of the strong organic sector in ***,if this law passes European parliament and council,unchanged,it will present a big step forward toward establishing a more sustainable European agricultural ***,it might soon be possible to develop and grow crops that are more adapted to global warming and whose cultivation will require lower amounts of ***,there is still a long way to go until the law is *** often,the storm of arguments raised by the opponents,based on irrational fears of mutations and a naive understanding of nature,has fallen on fruitful ground in Europe.

关键词： Genetically modified organism Cisgenesis Gene editing CRISPR/Cas Regulation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Broadening the applicability of CRISPR/Cas9 in plants

引用

Science China(Life Sciences) 2018年第1期61卷 126-127页

作者： holger Puchta Botanical Institute Karlsruhe Institute of Technology

The use of CRISPR（clustered regulatory interspaced short palindromic repeats）Cas9 is revolutionizing genome engineering—not only in plants（Schiml and Puchta,2016）.This is due to the fact that with high specificit... 详细信息

关键词： Broadening the applicability of CRISPR/Cas9 in plants NAG

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

How open is the therapeutic horizon for pancreatic cancer patients?

引用

Hepatobiliary & Pancreatic Diseases International 2022年第1期21卷 1-3页

作者： holger Kalthoff Institute for Experimental Cancer Research Christian-Albrechts-UniversityKielGerman

In the nineties of the last century,it was typical for an article about pancreatic cancer to start with an introductory remark,that this malignancy is a very desperate one and that surgery is the only curative *** it was also obvious that surgery was not really curative and most importantly,not feasible in the majority of *** the last two decades,we observed a dramatic increase in research on pancreatic cancer(*** than 65000 articles mentioning pancreatic cancer in PubMed only in the last decade),and clearly,there is scientific progress.

关键词： cancer curative surgery

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Computational biology approach to uncover hepatitis C virus helicase operation

引用

World Journal of Gastroenterology 2014年第13期20卷 3401-3409页

作者： holger Flechsig Department of Physical Chemistry Fritz Haber Institute of the Max Planck Society14195 BerlinGermany

Hepatitis C virus(HCV)helicase is a molecular motor that splits nucleic acid duplex structures during viral replication,therefore representing a promising target for antiviral ***,a detailed understanding of the mechanism by which it operates would facilitate the development of efficient drug-assisted therapies aiming to inhibit helicase *** extensive investigations performed in the past,a thorough understanding of the activity of this important protein was lacking since the underlying internal conformational motions could not be *** we review investigations that have been previously performed by us for HCV *** methods of structure-based computational modelling it became possible to follow entire operation cycles of this motor protein in structurally resolved simulations and uncover the mechanism by which it moves along the nucleic acid and accomplishes strand *** also discuss observations from that study in the light of recent experimental studies that confirm our findings.

关键词： Hepatitis C virus Viral replication Helicase protein Adenosine-triphosphate-induced operation Conformational motions Nucleic acid unzipping Computational biology Coarse-grained modelling Elastic-network model

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Progress in the research and application of coal mining with stowing

引用

International Journal of Mining Science and Technology 2013年第1期23卷 7-12页

作者： Junker Martin Witthaus holger RAG Mining Solutions GmbH Shamrockring 1 44623 Herne Germany

The development and achievements of modern coal mining with stowing are discussed in this paper. The necessity of developing coal mining with stowing is briefly summarized and internal damage within the strata and external damage on surface induced by longwall working with caving are discussed. Technological features of coal mining with pneumatic stowing in Germany, and fully mechanised coal mining with solid dense stowing in China, are discussed. In German hard coal mining stowing technologies are for technical, infrastructural and economical reasons not applicable. The discussion includes the stowing material transportation system, the stowing machine, and processes at the face. Progressiveness and application universality of China's fully mechanised coal mining with solid dense stowing are illustrated through practical examples with different conditions. Obviously, the fully mechanised coal mine with solid dense stowing can be improved by automaion systems. This paper discusses further developmental potential of the technology.

关键词： Coal mining with solid stowing Coal mining with pneumatic stowing Fully mechanised Coal mining with stowing in China and Germany

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Role of cytochrome P450 polymorphisms and functions in development of ulcerative colitis

引用

World Journal of Gastroenterology 2019年第23期25卷 2846-2862页

作者： Alaattin Sen holger Stark Department of Molecular Biology and Genetics Faculty of Life and Natural SciencesAbdullah Gul UniversityKayseri 38080Turkey Biology Department Faculty of Arts and SciencesPamukkale UniversityDenizli 20070Turkey Institute of Pharmaceutical and Medicinal Chemistry Heinrich Heine University DüsseldorfDuesseldorf 40225Germany

Cytochromes P450 s(CYPs) are terminal enzymes in CYP dependent monooxygenases, which constitute a superfamily of enzymes catalysing the metabolism of both endogenous and exogenous substances. One of their main tasks is to facilitate the excretion of these substances and eliminate their toxicities in most phase 1 reactions. Endogenous substrates of CYPs include steroids, bile acids, eicosanoids, cholesterol, vitamin D and neurotransmitters. About 80% of currently used drugs and environmental chemicals comprise exogenous substrates for CYPs. Genetic polymorphisms of CYPs may affect the enzyme functions and have been reported to be associated with various diseases and adverse drug reactions among different populations. In this review, we discuss the role of some critical CYP isoforms(CYP1 A1, CYP2 D6, CYP2 J2, CYP2 R1,CYP3 A5, CYP3 A7, CYP4 F3, CYP24 A1, CYP26 B1 and CYP27 B1) in the pathogenesis or aetiology of ulcerative colitis concerning gene polymorphisms. In addition, their significance in metabolism concerning ulcerative colitis in patients is also discussed showing a clear underestimation in genetic studies performed so far.

关键词： Cytochrome P450 Polymorphism Function Ulcerative colitis Aetiology

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Connection models for the Internet-of-Things

引用

Frontiers of Computer Science 2020年第3期14卷 65-85页

作者： Kangli HE holger HERMANNS Hengyang WU Yixiang CHEN MoE Engineering Research Center for Software/Hardware Co-design Technology and Application East China Normal UniversityShanghai 200062China Saarland University Saarland Informatics CampusSaarbrücken 66123Germany

The Internet-of-Things(IoT)is expected to swamp the *** order to study and understand the emergent behaviour of connected things,effective support for their modelling is *** the heart of IoT are flexible and adaptive connection patterns between things,which can naturally be modelled by channel-based coordination primitives,and characteristics of connection failure probabilities,execution and waiting times,as well as resource *** latter is especially important in light of severely limited power and computation budgets inside the *** this paper,we tackle the IoT modelling challenge,based on a conservative extension of channel-based Reo *** introduce a model called priced probabilistic timed constraint automaton,which combines models of probabilistic and timed aspects,and integrates pricing *** expressive logic called priced probabilistic timed scheduled data stream logic is presented,so as to enable the specification and verification of properties,which characterize data-flow streams and prices.A small but illustrative IoT case demonstrates the principal benefits of the proposed approach.

关键词： IoT Reo cost time probability automaton

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Quartic coupling and its effect on wetting behaviors in nematic liquid crystals

引用

Chinese Physics B 2015年第2期24卷 325-329页

作者：曾明颖 holger Merlitz 吴晨旭 Department of Physics and ITPA Xiamen University College of Information Science and Engineering Huaqiao University

Based on the fact that rubbed groove patterns also affect the anchoring of liquid crystals at substrates,a quartic coupling is included in constructing the surface energy for a liquid crystal *** phase diagram and the wetting behaviors of the liquid crystal cell,bounded by surfactant-laden interfaces in a magnetic field perpendicular to the substrate are discussed by taking the quartic coupling into *** nematic order increases at the surface while it decreases in the bulk as a result of the introduction of quartic substrate-liquid crystal coupling,indicating that the groove anchoring makes the liquid crystal molecules align more orderly near the substrate than away from *** causes a different wetting behavior：complete wetting.

关键词： quartic coupling surface anchoring energy phase transition wetting behavior

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of Local Nitrogen Supply on Water Uptake of Bean Plants in a Split Root System

引用

Journal of Integrative Plant Biology 2007年第4期49卷 472-480页

作者： Shiwei Guo Qirong Shen holger Brueckt College of Resource and Environmental Science Nanjing Agricultural University Nanjing 210095 China Institute of Plant Nutrition and Soil Science University of Kiel Kiel D-24118 Germany

To study the effects of local nitrogen supply on water and nutrient absorption, French bean （Phaseolus vulgaris L.） plants were grown in a split root system. Five treatments supplied with different nitrogen forms were compared： homogeneous nitrate （NN） and homogenous ammonium （AA） supply, spatially separated supply of nitrate and ammonium （NA）, half of the root system supplied with N-free nutrient solution, the other half with either nitrate （NO） or ammonium （AO）. The results showed that 10 d after onset of treatments, root dry matter （DM） in the nitratesupplied vessels treated with NA was more than two times higher than that in the ammonium-supplied vessels. Water uptake from the nitrate-supplied vessels treated with NA was 281% higher than under ammonium supply. In treatments NO and AO, the local supply of N resulted in clearly higher root DM, and water uptake from the nitratesupplied vessels was 82% higher than in the -N vessels. However, in AO plants, water uptake from the -N nutrient solution was 129% higher than from the ammonium-supplied vessels. This indicates a compensatory effect, which resulted in almost identical rates of total water uptake of treatments AA and AO, which had comparable shoot DM and leaf area. Ammonium supply reduced potassium and magnesium absorption. Water uptake was positively correlated with N, Mg and K uptake.

关键词： nitrogen form nutrient uptake rates Phaseolus vulgaris split root system water uptake rates.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：