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Journal of Huazhong University of Science and Technology(Medical Sciences) 2002年第2期22卷 158-163页

作者：杨娅王新房 Thomas Bartel holger eggebrecht Loredana Latina Clemems von Birgelen Guido Caspari Raimund Erbel Institute of Cardiovascular Disease Xiehe Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan Department of Cardiology University Essen Germany

Summary: Transthoracic Doppler echocardiography (TTDE) allows noninvasive flow measurement in the distal left anterior descending artery (LAD). The feasibility of detecting coronary flow by contrast-enhanced TTDE with second harmonic technique was assessed, the coronary flow velocity reserve (CFVR) was evaluated in comparison to intracoronary Doppler flow (ICD) analysis and the CFVR after PTCA in LAD was investigated. In 77 (96 %) of 80 patients, CFVR was successfully determined with intravenous adenosine infusion. Doppler signal quality was evaluated in the first 46 patients by use of intravenous Levovist infusion and second harmonic technique. The Doppler flow was not visible in 1 patient only. CFVR determined from TTDE (2.77±0.65) was correlated closely with those from ICD (2.88±0.78) measurements (y=0.73x+0.67, r=0.87, P<0.001). In conclusion, TTDE is a feasible method and provides reliable data on CFVR which can be used for follow-up after PTCA.

关键词： coronary flow velocity reserve Doppler echocardiography intracoronary Doppler

新型无创性冠状动脉血流储备检测经胸壁声学造影二次谐波多普勒技术

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中华心血管病杂志 2003年第2期31卷 91-95页

作者：杨娅王新房 Thomas Bartel holger eggebrecht Loredana Latina Raimund Erbel 华中科技大学同济医学院附属协和医院心血管病研究所德国埃森大学医学院心内科

目的　研究经胸壁声学造影二次谐波多普勒超声心动图探测左前降支 (LAD)冠状动脉血流储备 (CFVR)的可行性、安全性及可靠性。方法　 46例患者经静脉注射利声显 ,利用二次谐波观察LAD血流 ,对多普勒血流图像质量进行分级。CFVR为注射腺... 详细信息

目的　研究经胸壁声学造影二次谐波多普勒超声心动图探测左前降支 (LAD)冠状动脉血流储备 (CFVR)的可行性、安全性及可靠性。方法　 46例患者经静脉注射利声显 ,利用二次谐波观察LAD血流 ,对多普勒血流图像质量进行分级。CFVR为注射腺苷后的最大平均血流速度与静息状态平均血流速度的比值。结果　CFVR探测的成功率为 98%。注射腺苷后 ,心率加快 ,血压减低。60 %患者有轻微副反应。CFVR为 2 84± 0 65 ,与冠状动脉内多普勒探测的结果密切相关 (r=0 87,P <0 0 0 1 )。结论　经胸壁声学造影二次谐波多普勒超声心动图可明显提高冠状动脉血流探测的成功率 ,是安全、可行。

关键词：声学造影二次谐波多普勒技术血流储备冠状动脉超声心动图

Regulation of gene-edited plants in Europe:from the valley of tears into the shining sun?

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aBIOTECH 2024年第2期5卷 231-238页

作者： holger Puchta Department of Molecular Biology Joseph Gottlieb Kolreuter Institute for Plant SciencesKarlsruhe Institute of Technology(KIT)Fritz-Haber-Weg 476131 KarlsruheGermany

Some 20 years ago,the EU introduced complex regulatory rules for the growth of transgenic crops,which resulted in a de facto ban to grow these plants in fields within most European *** the rise of novel genome editing technologies,it has become possible to improve crops genetically in a directed way without the need for incorporation of foreign ***,in 2018,the European Court of Justice ruled that such gene-edited plants are to be regulated like transgenic *** then,European scientists and breeders have challenged this decision and requested a revision of this outdated ***,after 5 years,the European Commission has now published a proposal on how,in the future,to regulate crops produced by new breeding *** proposal tries to find a balance between the different interest groups in *** one side,genetically modified plants,which cannot be discerned from their natural counterparts,will exclusively be used for food and feed and are-besides a registration step-not to be regulated at *** the other side,plants expressing herbicide resistance are to be excluded from this regulation,a concession to the strong environmental associations and NGOs in ***,edited crops are to be excluded from organic farming to protect the business interests of the strong organic sector in ***,if this law passes European parliament and council,unchanged,it will present a big step forward toward establishing a more sustainable European agricultural ***,it might soon be possible to develop and grow crops that are more adapted to global warming and whose cultivation will require lower amounts of ***,there is still a long way to go until the law is *** often,the storm of arguments raised by the opponents,based on irrational fears of mutations and a naive understanding of nature,has fallen on fruitful ground in Europe.

关键词： Genetically modified organism Cisgenesis Gene editing CRISPR/Cas Regulation

Broadening the applicability of CRISPR/Cas9 in plants

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Science China(Life Sciences) 2018年第1期61卷 126-127页

作者： holger Puchta Botanical Institute Karlsruhe Institute of Technology

The use of CRISPR（clustered regulatory interspaced short palindromic repeats）Cas9 is revolutionizing genome engineering—not only in plants（Schiml and Puchta,2016）.This is due to the fact that with high specificit... 详细信息

关键词： Broadening the applicability of CRISPR/Cas9 in plants NAG

How open is the therapeutic horizon for pancreatic cancer patients?

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Hepatobiliary & Pancreatic Diseases International 2022年第1期21卷 1-3页

作者： holger Kalthoff Institute for Experimental Cancer Research Christian-Albrechts-UniversityKielGerman

In the nineties of the last century,it was typical for an article about pancreatic cancer to start with an introductory remark,that this malignancy is a very desperate one and that surgery is the only curative *** it was also obvious that surgery was not really curative and most importantly,not feasible in the majority of *** the last two decades,we observed a dramatic increase in research on pancreatic cancer(*** than 65000 articles mentioning pancreatic cancer in PubMed only in the last decade),and clearly,there is scientific progress.

关键词： cancer curative surgery

Computational biology approach to uncover hepatitis C virus helicase operation

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World Journal of Gastroenterology 2014年第13期20卷 3401-3409页

作者： holger Flechsig Department of Physical Chemistry Fritz Haber Institute of the Max Planck Society14195 BerlinGermany

Hepatitis C virus(HCV)helicase is a molecular motor that splits nucleic acid duplex structures during viral replication,therefore representing a promising target for antiviral ***,a detailed understanding of the mechanism by which it operates would facilitate the development of efficient drug-assisted therapies aiming to inhibit helicase *** extensive investigations performed in the past,a thorough understanding of the activity of this important protein was lacking since the underlying internal conformational motions could not be *** we review investigations that have been previously performed by us for HCV *** methods of structure-based computational modelling it became possible to follow entire operation cycles of this motor protein in structurally resolved simulations and uncover the mechanism by which it moves along the nucleic acid and accomplishes strand *** also discuss observations from that study in the light of recent experimental studies that confirm our findings.

关键词： Hepatitis C virus Viral replication Helicase protein Adenosine-triphosphate-induced operation Conformational motions Nucleic acid unzipping Computational biology Coarse-grained modelling Elastic-network model

Role of cytochrome P450 polymorphisms and functions in development of ulcerative colitis

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World Journal of Gastroenterology 2019年第23期25卷 2846-2862页

作者： Alaattin Sen holger Stark Department of Molecular Biology and Genetics Faculty of Life and Natural SciencesAbdullah Gul UniversityKayseri 38080Turkey Biology Department Faculty of Arts and SciencesPamukkale UniversityDenizli 20070Turkey Institute of Pharmaceutical and Medicinal Chemistry Heinrich Heine University DüsseldorfDuesseldorf 40225Germany

Cytochromes P450 s(CYPs) are terminal enzymes in CYP dependent monooxygenases, which constitute a superfamily of enzymes catalysing the metabolism of both endogenous and exogenous substances. One of their main tasks is to facilitate the excretion of these substances and eliminate their toxicities in most phase 1 reactions. Endogenous substrates of CYPs include steroids, bile acids, eicosanoids, cholesterol, vitamin D and neurotransmitters. About 80% of currently used drugs and environmental chemicals comprise exogenous substrates for CYPs. Genetic polymorphisms of CYPs may affect the enzyme functions and have been reported to be associated with various diseases and adverse drug reactions among different populations. In this review, we discuss the role of some critical CYP isoforms(CYP1 A1, CYP2 D6, CYP2 J2, CYP2 R1,CYP3 A5, CYP3 A7, CYP4 F3, CYP24 A1, CYP26 B1 and CYP27 B1) in the pathogenesis or aetiology of ulcerative colitis concerning gene polymorphisms. In addition, their significance in metabolism concerning ulcerative colitis in patients is also discussed showing a clear underestimation in genetic studies performed so far.

关键词： Cytochrome P450 Polymorphism Function Ulcerative colitis Aetiology

Progress in the research and application of coal mining with stowing

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International Journal of Mining Science and Technology 2013年第1期23卷 7-12页

作者： Junker Martin Witthaus holger RAG Mining Solutions GmbH Shamrockring 1 44623 Herne Germany

The development and achievements of modern coal mining with stowing are discussed in this paper. The necessity of developing coal mining with stowing is briefly summarized and internal damage within the strata and external damage on surface induced by longwall working with caving are discussed. Technological features of coal mining with pneumatic stowing in Germany, and fully mechanised coal mining with solid dense stowing in China, are discussed. In German hard coal mining stowing technologies are for technical, infrastructural and economical reasons not applicable. The discussion includes the stowing material transportation system, the stowing machine, and processes at the face. Progressiveness and application universality of China's fully mechanised coal mining with solid dense stowing are illustrated through practical examples with different conditions. Obviously, the fully mechanised coal mine with solid dense stowing can be improved by automaion systems. This paper discusses further developmental potential of the technology.

关键词： Coal mining with solid stowing Coal mining with pneumatic stowing Fully mechanised Coal mining with stowing in China and Germany

Connection models for the Internet-of-Things

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Frontiers of Computer Science 2020年第3期14卷 65-85页

作者： Kangli HE holger HERMANNS Hengyang WU Yixiang CHEN MoE Engineering Research Center for Software/Hardware Co-design Technology and Application East China Normal UniversityShanghai 200062China Saarland University Saarland Informatics CampusSaarbrücken 66123Germany

The Internet-of-Things(IoT)is expected to swamp the *** order to study and understand the emergent behaviour of connected things,effective support for their modelling is *** the heart of IoT are flexible and adaptive connection patterns between things,which can naturally be modelled by channel-based coordination primitives,and characteristics of connection failure probabilities,execution and waiting times,as well as resource *** latter is especially important in light of severely limited power and computation budgets inside the *** this paper,we tackle the IoT modelling challenge,based on a conservative extension of channel-based Reo *** introduce a model called priced probabilistic timed constraint automaton,which combines models of probabilistic and timed aspects,and integrates pricing *** expressive logic called priced probabilistic timed scheduled data stream logic is presented,so as to enable the specification and verification of properties,which characterize data-flow streams and prices.A small but illustrative IoT case demonstrates the principal benefits of the proposed approach.

关键词： IoT Reo cost time probability automaton

Quartic coupling and its effect on wetting behaviors in nematic liquid crystals

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Chinese Physics B 2015年第2期24卷 325-329页

作者：曾明颖 holger Merlitz 吴晨旭 Department of Physics and ITPA Xiamen University College of Information Science and Engineering Huaqiao University

Based on the fact that rubbed groove patterns also affect the anchoring of liquid crystals at substrates,a quartic coupling is included in constructing the surface energy for a liquid crystal *** phase diagram and the wetting behaviors of the liquid crystal cell,bounded by surfactant-laden interfaces in a magnetic field perpendicular to the substrate are discussed by taking the quartic coupling into *** nematic order increases at the surface while it decreases in the bulk as a result of the introduction of quartic substrate-liquid crystal coupling,indicating that the groove anchoring makes the liquid crystal molecules align more orderly near the substrate than away from *** causes a different wetting behavior：complete wetting.

关键词： quartic coupling surface anchoring energy phase transition wetting behavior