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Thioredoxin Reductase Type C （NTRC） Orchestrates Enhanced Thermotolerance to Arabidopsis by Its Redox-Dependent holdase Chaperone Function

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Molecular Plant 2013年第2期6卷 323-336页

作者： ho byoung chae Jeong Chan Moon Mi Rim Shin Yong Hun Chi Young Jun Jung Sun Yong Lee Ganesh M. Nawkar Hyun Suk Jung Jae Kyung Hyun Woe Yeon Kim Chang ho Kang Dae-Jin Yun Kyun Oh Lee Sang Yeol Lee Division of Applied Life Science (BK21 Program) and PMBBRC Gyeongsang National University 501 Jinju-daero Jinju 660-701 Korea Division of Electron Microscopic Research Korea Basic Science Institute 113 Gwahangno Yuse0ng-guDaejeon 303-333 Korea

Genevestigator analysis has indicated heat shock induction of transcripts for NADPH-thioredoxin reduc-tase, type C （NTRC） in the light. Here we show overexpression of NTRC in Arabidopsis （NTRC°E） resulting in enhanced tolerance to heat shock, whereas NTRC knockout mutant plants （ntrcl） exhibit a temperature sensitive phenotype. To investigate the underlying mechanism of this phenotype, we analyzed the protein＇s biochemical properties and protein structure. NTRC assembles into homopolymeric structures of varying complexity with functions as a disulfide reductase, a foldase chaperone, and as a holdase chaperone. The multiple functions of NTRC are closely correlated with protein structure.. Complexes of higher molecular weight （HMW） showed stronger activity as a holdase chaperone, while low molecular weight （LMW） species exhibited weaker holdase chaperone activity but stronger disulfide reductase and fol-dase chaperone activities. Heat shock converted LMW proteins into HMW complexes. Mutations of the two active site Cys residues of NTRC into Ser （C217/454S-NTRC） led to a complete inactivation of its disulfide reductase and foldase chaperone functions, but conferred only a slight decrease in its holdase chaperone function. The overexpression of the mutated C217/454S-NTRC provided Arabidopsis with a similar degree of thermotolerance compared with that of NTRC°E plants. however, after prolonged incubation under heat shock, NTRC°E plants tolerated the stress to a higher degree than C217/454S-NTRC°E plants. The results suggest that the heat shock-mediated holdase chaperone function of NTRC is responsible for the increased thermotolerance of Arabidopsis and the activity is significantly supported by NADPH.

关键词： NADPH-thioredoxin reductase type C (NTRC) oligomeric complexes disulfide reductase foldase and holdasechaperone functions redox thermotolerance.

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Redox sensor QSOX1 regulates plant immunity by targeting GSNOR to modulate ROS generation

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Molecular Plant 2021年第8期14卷 1312-1327页

作者： ho byoung chae Min Gab Kim Chang ho Kang Joung Hun Park Eun Seon Lee Sang-Uk Lee Yong Hun Chi Seol Ki Paeng Su Bin Bae Seong Dong Wi Byung-Wook Yun Woe-Yeon Kim Dae-Jin Yun David Mackey Sang Yeol Lee Division of Applied Life Sciences(BK21)and PMBBRC Gyeongsang National UniversityJinju 52828Korea College of Pharmacy Research Institute of Pharmaceutical ScienceGyeongsang National UniversityJinju 52828Korea School of Applied Biosciences College of Agriculture and Life SciencesKyungpook National UniversityDaegu 41566Korea Department of Biomedical Science and Engineering Konkuk UniversitySeoul 05029Korea Department of horticulture and Crop Science Department of Molecular Geneticsand Center for Applied Plant SciencesThe Ohio State UniversityColumbusOH 43210USA College of Life Sciences Qingdao Agricultural UniversityQingdao 266109P.R.China Plant Propagation Team Plant Production DivisionSejong National ArboretumSejong 30106Korea

Reactive oxygen signaling regulates numerous biological processes,including stress responses in *** sensors transduce reactive oxygen signals into cellular ***,we present biochemical evidence that a plant quiescin sulfhydryl oxidase homolog(QSOX1)is a redox sensor that negatively regulates plant immunity against a bacterial *** expression level of QSOX1 is inversely correlated with pathogen-induced reactive oxygen species(ROS)***,QSOX1 both senses and regulates ROS levels by interactingn with and mediating redox regulation of S-nitrosoglutathione reductase,which,consistent with previous findings,influences reactive nitrogen-mediated regulation of ROS ***,our data indicate that QSOX1 is a redox sensorthat negatively regulates plant immunity by linking reactive oxygen and reactive nitrogen signaling to limit ROS production.

关键词： redox sensor plant immu nity QSOX1 GSNOR reactive oxygen species(ROS) reactive nitrogen species(RNS)

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Improved thermal performance of a hydronic radiant panel heating system by the optimization of tube shapes

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2011年第6期12卷 428-437页

作者： Young T.chae Kwang ho LEE Jae Sung PARK School of Architecture University of Illinois at Urbana-Champaign Department of Architectural Engineering Hanbat National University Department of Mechanical Engineering University of Illinois at Urbana-Champaign

The thermal performance enhancement of the hydronic radiant floor heating system by tube shape refinements is investigated in this *** analytical and detailed numerical modelings are carried out to predict the performance of the radiant *** the simple analytical model briefly investigates the possibility of the effect of the tube shape improvement with the parametric analysis,the commercial computational fluid dynamics(CFD) code(Ansys/CFX) is used to perform the detailed 3D analysis under different tube shape *** fin thickness,the number of fins,and the tube thermal conductivity turn out to have significant effects on the radiant system *** potential energy saving impacts of the tube shape refinements are also *** tube shape improvement turns out to increase the floor surface temperature and to decrease the hot water temperature drop across the system,resulting in heating energy savings.

关键词： Radiant panel system Analytical modeling Computational fluid dynamics(CFD) Tube shape Energy saving

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A novel electrospinning method for self-assembled tree-like fibrous scaffolds:Microenvironment-associated regulation of MSC behavior and bone regeneration

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Journal of Materials Science & Technology 2022年第20期115卷 52-70页

作者： Jeong In Kim Ju Yeon Kim Sung-ho Kook Jeong-chae Lee Cluster for Craniofacial Development and Regeneration Research Institute of Oral Biosciences and School of DentistryJeonbuk National UniversityJeonju 54896Republic of Korea Department of Bionanosystem Engineering Jeonbuk National UniversityJeonju 54896Republic of Korea Department of Bioactive Material Sciences Research Center of Bioactive MaterialsJeonbuk National UniversityJeonju 54896Republic of Korea

Numerous studies highlight advantages of electrospun scaffolds in bone tissue engineering,in which cellular behavior is tightly affected by fiber topographical cues of ***,the classic electrospinning setup limits a desired presentation of biomimetic fibrous microenvironments that sense mechanosignaling and regulate stem cell *** aims of this study were to fabricate advanced asspun scaffolds presenting tree-like microfiber/nanonet networks and to evaluate their regulatory potentials on behavior of human mesenchymal stem cells(h MSCs)and bone *** we developed a novel electrospinning setup that allowed the presentation of patterned Trunk microfibers(TMF)and/or branched nanonet fibers(BNn Fs)in biomimetic fibrous *** the cellular mechanisms,anisotropichierarchical topography of TMF controlled behavior of h MSCs through focal adhesion formation and Yesassociated protein(YAP)induction,whereas BNn F disturbed such mechanosensing responses in the *** fiber microenvironment-related expression and nuclear localization of YAP *** correlated with the potentials of as-spun scaffolds to enhance osteogenic differentiation of the h MSCs and alveolar bone defect healing in an animal ***,this study provides an advanced approach of the modified electrospinning setup for presentation of biomimetic fibrillar microenvironments in as-spun scaffolds along with their application in stem cell behavior regulation and regenerative tissue engineering.

关键词： Electrospinning Advanced biomimetic scaffolds Stem cell behavior Mechanosensing Bone tissue engineering

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Application of Integrated Database to the Casting Design

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Journal of Materials Science & Technology 2008年第3期24卷 351-355页

作者： In-Sung Cho Seung-Mok Yoo chae-ho Lim Jeong-Kil Choi KITECH 7-47 Songdo-dongYeonsu-gu

Construction of integrated database including casting shapes with their casting design, technical knowledge, and thermophysical properties of the casting alloys were introduced in the present study. Recognition tech- nique for casting design by industrial computer tomography was used for the construction of shape database. Technical knowledge of the casting processes such as ferrous and non-ferrous alloys and their manufacturing process of the castings were accumulated and the search engine for the knowledge was developed. Database of thermophysical properties of the casting alloys were obtained via the experimental study, and the properties were used for the in-house computer simulation of casting process. The databases were linked with intelligent casting expert system developed in center for e-design, KITECH. It is expected that the databases can help non casting experts to devise the casting and its process. Various examples of the application by using the databases were shown in the present study.

关键词： Integrated database Casting design Shape recognition

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A Study of the Gas Flow through Air Jet Loom

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Journal of Thermal Science 2007年第2期16卷 159-163页

作者： Heuy-Dong Kim chae-Min Lim ho-Joon Lee Doo-Hwan Chun School of Mechanical Engineering Andong National University388Songcheon-dongAndong 760-749KoreaE-mail:kimhd Korea Textile Machinery Research Institute 300Sampoong-dongKyeongsan 712-210Korea

Air jet loom, as one of the shuttleless looms, transports a yam into warps using viscosity and kinetic energy of an air jet. Performance of this picking system depends on the ability of instantaneous inhalation/exhaust, configuration of nozzle, operation characteristics of a check valve, etc. In the recent past, many studies have been reported on the air jet discharged from a nozzle exit, but studies for understanding the flow field characteristics associated with shear layer and shock wave/boundary layer interaction in the nozzle were not conducted enough. In this paper, a computational study was performed to explain the flow field in the air jet nozzle with an acceleration tube and validated with previous experimental data available. The results obtained from the computational study show that, in the supersonic flow regime, the flow field depends significantly on the length of acceleration tube. As nozzle pressure ratio increases, drag force acting on the string also increases. For a longer acceleration tube, the total pressure loss is large, owing to the frictional loss.

关键词： compressible flow nozzle shear layer shock wave air jet loom shuttleless loom

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均相和非均相催化微藻脂质提取和酯交换制备生物燃料的研究进展

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Chinese Journal of Catalysis 2024年第4期59卷 97-117页

作者： Vinoth Kumar Ponnumsamy Hussein E.Al-Hazmi Sutha Shobana Jeyaprakash Dharmaraja Dipak Ashok Jadhav Rajesh Banu J Grzegorz Piechota Bartłomiej Igliński Vinod Kumar Amit Bhatnagar Kyu-Jung chae Gopalakrishnan Kumar 高雄医科大学药物与应用化学系环境医学研究中心中国台湾高雄高雄医科大学医学研究部中国台湾高雄格但斯克理工大学土木与环境工程学院波兰纳鲁特维察格但斯克范朗大学工程与技术学院建筑绿色技术与可持续发展研究组越南胡志明市工程技术学院科学与人文系化学部印度泰米尔纳德邦韩国海洋大学海洋科学与工程学院环境工程系韩国釜山泰米尔纳德邦中央大学生命科学系印度泰米尔纳德邦 GPCHEM沼气研究与分析实验室波兰尼古拉斯哥白尼大学化学系波兰克兰菲尔德大学水与能源学院英国拉彭兰塔工业大学工程科学学院分离科学系芬兰韩国海洋大学海洋可再生能源工程专业韩国釜山延世大学土木与环境工程学院韩国首尔斯塔万格大学科学技术学院化学、生物科学与环境工程研究所挪威斯塔万格

随着化石燃料燃烧导致的二氧化碳排放不断加剧气候变化,且化石燃料储量日益减少,寻求可再生能源已成为一项紧迫的任务.其中,藻类衍生可持续燃料因具有成本优势和可运输性,在解决全球能源危机方面展现出广阔前景,备受关注.利用化学转化... 详细信息

随着化石燃料燃烧导致的二氧化碳排放不断加剧气候变化,且化石燃料储量日益减少,寻求可再生能源已成为一项紧迫的任务.其中,藻类衍生可持续燃料因具有成本优势和可运输性,在解决全球能源危机方面展现出广阔前景,备受关注.利用化学转化技术从微藻中提取脂质,并通过酯交换反应可以将其转化为脂肪酸甲酯,是生产绿色生物燃料的有效途径.这一过程涉及游离脂肪酸、磷脂和甘油三酯的提取,并且生产过程能耗低,成为满足日益增长的能源需求的一种理想解决方案.本文综述了微藻脂质提取和酯交换制备生物燃料的相关研究进展.首先,介绍了微藻脂质的提取方法,包括溶剂提取法、索氏提取法、布利格和戴耶法、超临界二氧化碳提取以及离子液体溶剂法等,并分析了各方法的优缺点.随后,重点阐述了酯交换技术在微藻脂质转化中的应用,包括酸碱催化、酶催化以及原位酯交换反应等,并探讨了这些技术的反应机理、催化剂选择、反应器设计以及生物油生产工艺等方面的研究进展.通过综述上述研究进展,为微藻脂质的生产和应用提供了理论指导.研究表明,通过优化催化剂种类、反应条件以及提取方法,可以有效提高微藻脂质的转化效率和生物油品质.同时,本文也指出了当前微藻脂质生产中面临的挑战,如微藻栽培和生长条件优化、高效转化技术的开发等.随着可持续能源日益受到重视,微藻脂质作为一种可再生能源具有巨大的发展潜力.未来研究应进一步关注微藻的规模化栽培、生长条件优化以及高效转化技术的研发,以提高微藻脂质的产量和品质.同时,应进一步推动和实现微藻生物燃料的实际应用,从而为应对气候变化和能源危机提供有效的解决方案.

关键词：微藻脂质提取酯交换催化酶催化原位技术

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Evaluation of defects in aluminum piston castings by using ultrasonics and computer tomography

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China Foundry 2012年第3期9卷 275-278页

作者： In-Sung Cho Jeong-ho Hwang Jin-Seok Yang Seung-Mok Yoo chae-ho Lim Korea Institute of Industrial Technology

Pistons used in automobile engines are made of aluminum alloy; and endurance of high pressure and high temperature is required. Recently, high strength pistons are needed to cope with the increase of pressure and temperature in the engine cylinder. A high strength piston is possible when the piston casting has little or no casting defects, such as micro-pores and cracks. Generally the defects can be evaluated by non-destructive testing (NDT), and the most efficient ways for evaluation are using ultrasonics and computer tomography. In the present study, two NDT methods were compared and evaluated to investigate the defects in the aluminum piston castings. Artificial defects were machined by using very small drill bits with diameters 1, 0.5, 0.3, 0.2 and 0.1 mm. The defects were investigated by using an ultrasonic tester and a computer tomography system. Defects smaller than 0.3 mm may not be found by using the ultrasonic test, but the defects may be found by using the computer tomography system. The investigation conditions for detecting small defects and the scanning time for in-line defect analysis are discussed in the present study.

关键词： piston ultrasonic test computer tomography aluminum defect

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Effects of acupuncture stimulation on brain activation induced by cue-elicited alcohol craving

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Neural Regeneration Research 2022年第5期17卷 1059-1064页

作者： Hyeok Gyu Kwon Seong Hun Choi Joon ho Seo chae Ha Yang Mi Young Lee Department of Physical Therapy College of Health ScienceEulji UniversityGyeonggiRepublic of Korea Department of Anatomy and Histology College of Oriental MedicineDaegu Haany UniversityGyeongsanRepublic of Korea Department of Rehabilitation Gyeongbuk Regional Rehabilitation HospitalGyeongsanRepublic of Korea Department of Physiology College of Korean MedicineDaegu Haany UniversityDaeguRepublic of Korea Department of Physical Therapy College of Biomedical ScienceDaegu Haany UniversityGyeongsanRepublic of Korea

Acupuncture has been shown to be effective on alcohol use ***,the underlying mechanism remains poorly *** investigate the effects of Shenmen(HT7)acupoint on brain activation induced by cue-elicited alcohol craving,30 right-handed healthy light to moderate alcohol drinkers were recruited from the *** were randomly assigned to undergo acupuncture either at HT7(experimental acupoint,n=15)or Jingqu(LU8,control acupoint,n=15)*** randomized controlled study was performed in Daegu Haany University and Daegu-Gyeongbuk Medical Innovation Foundation,Republic of *** and data collection were conducted from December 2018 to May *** results showed that after acupuncture at HT7 acupoint,the activation of orbitofrontal cortex and dorsolateral prefrontal cortex was greatly increased,while the activation of dorsolateral prefrontal cortex was obviously reduced,and subject's craving for alcohol was reduced when he/she seeing alcohol-related video clips involving various alcohols(beer,wine,or soju)or drinking *** at HT7 more greatly reduced subject's alcohol cravings than acupuncture at LU8 *** findings suggest that acupuncture can improve the self-control of mild to moderate social drinkers through the activation of the orbitofrontal cortex and dorsolateral prefrontal cortex,thereby reducing the craving for *** study protocol was approved by the Institutional Review Board of Daegu Haany University Korean Medicine hospital,Republic of Korea(approval ***-D-18026-PRO-02)on November 30,2018.

关键词： acupuncture addiction alcohol brain activation craving cue functional MRI Shenmen(HT7)acupoint

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Electrochemically metal-doped reduced graphene oxide films:Properties and applications

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Journal of Materials Science & Technology 2020年第5期40卷 72-80页

作者： Myung-Sic chae Tae ho Lee Kyung Rock Son Tae hoon Park Kyo Seon Hwang Tae Geun Kim School of Electrical Engineering Korea UniversityAnam-ro 145Seongbuk-guSeoul 02841South Korea Department of Clinical Pharmacology and Therapeutics College of MedicineKyung Hee UniversitySeoul 02447South Korea

The fine control of doping levels in graphene materials such as reduced graphene oxide(RGO)is important to properly manipulate their ambipolar transport characteristics for various device ***,conventional doping methods involve complex chemical reactions,large-scale doping processes,and poor ***,a simple and controllable electrochemical doping treatment(EDT),performed via the conductive channels created at the RGO surface by the application of an electric field,is introduced to tailor the electrical properties of RGO films.X-ray photoelectron spectroscopy and Raman spectroscopy measurements are performed to detect the presence of Ni atoms in RGO films after the EDT(EDT-RGO).Then,EDT-RGO field-effect transistors(FETs)are fabricated with different doping areas(0 to 100%fractional area)on the RGO active channel to investigate the effect and selective-area doping capability of the *** to p-type doping compensation by the intercalated Ni atoms,the electron mobility of the EDT-RGO FET decreases from 1.40 to 0.12 cm2 V-1s-1 compared with that of the undoped RGO-FET,leading to the conversion from ambipolar to unipolar p-type transfer characteristics.

关键词： Reduced graphene oxide Electrochemical doping treatment Electrical breakdown process Field-effect transistor

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