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Dexamethasone inhibits hypoxia-induced epithelial-mesenchymal transition in colon cancer

World Journal of Gastroenterology 2015年第34期21卷 9887-9899页

作者： Jung ho Kim You-Jin hwang Sang hoon Han Young Eun Lee Saerom Kim Yoon Jae Kim Jae Hee Cho Kwang An Kwon Ju Hyun Kim Se-Hee Kim Department of Internal Medicine Gachon University Gil Medical Center Division of Biological Science Gachon University of Medicine and Science Department of Internal Medicine Jeju National University School of Medicine Gachon Medical Research Institute Gachon University Gil Medical Center

AIM:To elucidate the effects of dexamethasone on hypoxia-induced epithelial-to-mesenchymal transition(EMT) in colon ***:Human colon cancer HCT116 and HT29 cells were exposed to normoxic(21%) and hypoxic(1%) conditions. First,the effect of dexamethasone on cell viability was examined by MTT cell proliferation assay. In order to measure the expression levels of EMT markers(Snail,Slug,Twist,E-cadherin,and integrin αVβ6) and hypoxia-related genes [Hypoxia-inducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF)] by dexamethasone,quantitative real-time polymerase chain reaction and western blot analysis were performed. Furthermore,the morphological changes of colon cancer cells and the expression pattern of E-cadherin by dexamethasone were detected through immunocytochemistry. Finally,the effects of dexamethasone on the invasiveness and migration of colon cancer cells were elucidated using matrigel invasion,migration,and wound healing migration ***:Under hypoxia,dexamethasone treatment inhibited HIF-1α protein level and its downstream gene,VEGF m RNA level in the colon cancer cell lines,HCT116 and HT29. In addition,the presence of dexamethasone down-regulated the m RNA levels of hypoxia-induced Snail,Slug,and Twist,all transcriptional factors of EMT,as well as hypoxia-induced integrin αVβ6 protein level,a well-known EMT marker for colon cancer cells. Furthermore,reduced E-cadherin in hypoxic condition was found to be recoverable by treating with dexamethasone in both colon cancer cell lines. Similarly,under hypoxic conditions,dexamethasone restored the growth pattern and morphological phenotype reminiscent of colon cancer cells grown under normoxic conditions; dexamethasone blocked the migration and invasion of both colorectal cancer cell lines in ***:Our study suggested that dexamethasone has inhibitory effects on cell migration and invasion by suppressing EMT of colon cancer cell lines in hypoxic condition.

关键词： Dexamethasone Colon cancer Hypoxia Hypoxia-inducib

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Non-surgical treatment of post-surgical bile duct injury: Clinical implications and outcomes

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World Journal of Gastroenterology 2014年第22期20卷 6924-6931页

作者： Young Ook Eum Joo Kyung Park Jaeyoung Chun Sang-Hyub Lee Ji Kon Ryu Yong-Tae Kim Yong-Bum Yoon Chang Jin Yoon ho-Seong Han Jin-Hyeok hwang Department of Internal medicine Cheongju St. Mary's HospitalChungcheongbuk-do 360-568South Korea Department of Internal Medicine Seoul National University Hospital Seoul National University College of Medicine Seoul 110-744South Korea Department of Internal Medicine Seoul National University College of Medicine Seoul National University Bundang hospital Gyeonggi-do 463-707South Korea Department of Radiology Seoul National University Bundang HospitalGyeonggi-do 463-707South Korea Department of Surgery Seoul National University Bundang HospitalGyeonggi-do 463-707South Korea

AIM: To investigate the prognostic factors determining the success rate of non-surgical treatment in the management of post-operative bile duct injuries (BDIs).

关键词： Endoscopic retrograde cholangiography Percutaneous transhepatic cholangiography Percutaneous transhepatic biliary drainage Bile duct Biliary stricture.

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Neurons in the hippocampal CA1 region,but not the dentate gyrus,are susceptible to oxidative stress in rats with streptozotocin-induced type 1 diabetes

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Neural Regeneration Research 2015年第3期10卷 451-456页

作者： Sang Gun Lee Dae Young Yoo Hyo Young Jung Sung Min Nam Jong Whi Kim Jung hoon Choi Sun Shin Yi Moo-ho Won Yeo Sung Yoon In Koo hwang Seung Myung Moon Departments of Neurosurgery Dongtan Sacred Heart HospitalCollege of MedicineHallym University Department of Anatomy and Cell Biology College of Veterinary Medicineand Research Institute for Veterinary ScienceSeoul National University Department of Anatomy College of Veterinary MedicineKangwon National University Department of Biomedical Laboratory Science College of Medical SciencesSoonchunhyang University Department of Neurobiology School of MedicineKangwon National University

In this study, we investigated the effects of streptozotocin-induced type 1 diabetes on antioxi- dant-like protein-1 immunoreactivity, protein carbonyl levels, and malondialdehyde formation, a marker for lipid peroxidation, in the hippocampus. For this study, streptozotocin （75 mg/kg） was intraperitoneally injected into adult rats to induce type 1 diabetes. The three experimental pa- rameters were determined at 2, 3, 4 weeks after streptozotocin treatment. Fasting blood glucose levels significantly increased by 20.7-21.9 mM after streptozotocin treatment. The number of antioxidant-like protein-1 immunoreactive neurons significantly decreased in the hippocampal CA1 region, but not the dentate gyrus, 3 weeks after streptozotocin treatment compared to the control group. Malondialdehyde and protein carbonyl levels, which are modified by oxidative stress, significantly increased with a peak at 3 weeks after malondialdehyde treatment, and then decreased 4 weeks after malondialdehyde treatment. These results suggest that neurons in the hippocampal CA1 region, but not the dentate gyrus, are susceptible to oxidative stress 3 weeks after malondialdehyde treatment.

关键词： nerve regeneration hippocampus dentate gyrus lipid peroxidation type 1 diabetes malondialdehyde neurons neural regeneration

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Inhibiting scar formation via wearable multilayer stacked electret patch:Self-creation of persistent and customizable DC electric field for fibrogenic activity restriction

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InfoMat 2024年第3期6卷 66-80页

作者： Sung-Won Kim Sumin Cho Donghan Lee Jiyu Hyun Sunmin Jang Inwoo Seo Hyun Su Park Hee Jae hwang Hyung-Seop Han Dae Hyeok Yang Heung Jae Chun Suk ho Bhang Dongwhi Choi School of Chemical Engineering Sungkyunkwan UniversitySuwonRepublic of Korea Department of Mechanical Engineering(Integrated Engineering Program) Kyung Hee UniversityYonginGyeonggiRepublic of Korea Biomaterials Research Center Biomedical Research DivisionKorea Institute of Science and Technology(KIST)SeoulRepublic of Korea Department of Medical Life Sciences College of MedicineInstitute of Cell and Tissue EngineeringThe Catholic University of KoreaSeoulRepublic of Korea

Electrical stimulation has recently received attention as noninvasive treatment in skin wound healing with its outstanding biological property for clinical ***,the complexity of equipment for applying appropriate electrical stimulation remains an ongoing ***,we proposed a strategy for skin scar inhibition by providing electrical stimulation via a multilayer stacked electret(MS-electret),which can generate direct current(DC)electric field(EF)without any power supply *** addition,the MS-electret can easily control the intensity of EFs by simply stacking electret layers and maintain stable EF with the surface potential of 3400 V over 5 days owing to the injected charges on the electret *** confirmed inhibition of type 1 collagen andα-SMA expression of human dermal fibroblasts(hDFs)by 90%and 44%in vitro,indicating that the transition of hDFs to myofibroblasts was restricted by applying stable electrical *** further revealed a 20%significant decrease in the ratio of myofibroblasts caused by the MS-electret in *** findings present that the MS-electret is an outstanding candidate for effective skin scar inhibition with a battery-free,physiological electrical microenvironment,and noninvasive treatment that allows it to prevent external infection.

关键词： DC electric field dermal fibroblasts electret scar formation restriction self-creation of electrical stimulation

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Cinnamon extract suppresses experimental colitis through modulation of antigen-presenting cells

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World Journal of Gastroenterology 2011年第8期17卷 976-986页

作者： ho-Keun Kwon Ji-Sun hwang Choong-Gu Lee Jae-Seon So Anupama Sahoo Chang-Rok Im Won Kyung Jeon Byoung Seob Ko Sung Haeng Lee Zee Yong Park Sin-Hyeog Im School of Life Sciences and Immune Synapse Research Center Gwangju Institute of Science and Technology Global leader program Bugil Academy Cheonan Korea Institute of Orien-tal Medicine Chosun University School of Medicine

AIM:To investigate the anti-inflammatory effects of cinnamon extract and elucidate its mechanisms for targeting the function of antigen presenting ***:Cinnamon extract was used to treat murine macrophage cell line(Raw 264.7),mouse primary antigen-presenting cells(APCs,MHCII+) and CD11c+dendritic cells to analyze the effects of cinnamon extract on APC *** mechanisms of action of cinnamon extract on APCs were investigated by analyzing cytokine production,and expression of MHC antigens and co-stimulatory molecules by quantitative real-time PCR and flow *** addition,the effect of cinnamon extract on antigen presentation capacity and APC-dependent T-cell differentiation were analyzed by [H3]-thymidine incorporation and cytokine analysis,*** confirm the anti-inflammatory effects of cinnamon extract in vivo,cinnamon or PBS was orally administered to mice for 20 d followed by induction of experimental colitis with 2,4,6 trinitrobenzenesulfonic *** protective effects of cinnamon extract against experimental colitis were measured by checking clinical symptoms,histological analysis and cytokine expression prof iles in inflamed ***:Treatment with cinnamon extract inhibited maturation of MHCII+ APCs or CD11c+ dendritic cells(DCs) by suppressing expression of co-stimulatory molecules(B7.1,B7.2,ICOS-L),MHCII and cyclooxygenase(COX)-*** extract induced regulatory DCs(rDCs) that produce low levels of pro-inflammatory cytokines [interleukin(IL)-1β,IL-6,IL-12,interferon(IFN)-γ and tumor necrosis factor(TNF)-α] while expressing high levels of immunoregulatory cytokines(IL-10 and transforming growth factor-β).In addition,rDCs generated by cinnamon extract inhibited APC-dependent T-cell proliferation,and converted CD4+ T cells into IL-10high CD4+ T ***,oral administration of cinnamon extract inhibited development and progression of intestinal colitis by inhibiting expression of COX-2 and pro-inflammatory cytokin

关键词： Cinnamon extract Inflammation CD4 antigen Antigen presenting cells Cyclooxygenase-2 Tumor necrosis factor-α Interleukin-10 Inflammatory bowel disease

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Vertical plane depth-resolved surface potential and carrier separation characteristics in flexible CZTSSe solar cells with over 12% efficiency

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Carbon Energy 2024年第3期6卷 36-52页

作者： Dae-ho Son Ha Kyung Park Dae-Hwan Kim Jin-Kyu Kang Shi-Joon Sung Dae-Kue hwang Jaebaek Lee Dong-Hwan Jeon Yunae Cho William Jo Taeseon Lee Junho Kim Sang-hoon Nam Kee-Jeong Yang Division of Energy Technology DGISTDaeguRepublic of Korea Research Center for Thin Film Solar Cells DGISTDaeguRepublic of Korea Department of Physics Ewha Womans UniversitySeoulRepublic of Korea Department of Physics Incheon National UniversityIncheonRepublic of Korea Department of Mechanical Engineering Massachusetts Institute of TechnologyCambridgeMassachusettsUSA

Cu2ZnSn(S,Se)4(CZTSSe)solar cells have resource distribution and economic *** main cause of their low efficiency is carrier loss resulting from recombination of photo-generated electron and *** overcome this,it is important to understand their electron-hole behavior *** determine the carrier separation characteristics,we measured the surface potential and the local current in terms of the absorber *** elemental variation in the intragrains(IGs)and at the grain boundaries(GBs)caused a band edge shift and bandgap(Eg)*** the absorber surface and subsurface,an upward Ec and Ev band bending structure was observed at the GBs,and the carrier separation was *** the absorber center,both upward Ec and Ev and downward Ec-upward Ev band bending structures were observed at the GBs,and the carrier separation was *** improve the carrier separation and suppress carrier recombination,an upward Ec and Ev band bending structure at the GBs is desirable.

关键词： carrier separation CZTSSe flexible solar cell local current surface potential

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Negative impact of sedation on esophagogastric junction evaluation during esophagogastroduodenoscopy

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World Journal of Gastroenterology 2014年第18期20卷 5527-5532页

作者： Eun Soo Kim ho Young Lee Yoo Jin Lee Bo Ram Min Jae Hyuk Choi Kyung Sik Park Kwang Bum Cho Byoung Kuk Jang Woo Jin Chung Jae Seok hwang Division of Gastroenterology and Hepatology Department of Internal MedicineKeimyung University School of MedicineDaegu 700-712South Korea

AIM: To compare the esophagogastric junction (EGJ) areas observed in sedated and non-sedated patients during esophagogastroduodenoscopy (EGD).

关键词： Esophagogastric junction Endoscopy Esophagus Propofol Midazolam

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Long-term administration of scopolamine interferes with nerve cell proliferation, differentiation and migration in adult mouse hippocampal dentate gyrus, but it does not induce cell death

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Neural Regeneration Research 2014年第19期9卷 1731-1739页

作者： Bing Chun Yan Joon Ha Park Bai Hui Chen Jeong-Hwi Cho In Hye Kim Ji Hyeon Ahn Jae-Chul Lee In Koo hwang Jun Hwi Cho Yun Lyul Lee Il-Jun Kang Moo-ho Won Department of Integrative Traditional & Western Medicine Medical CollegeYangzhou University Department of Neurobiology School of MedicineKangwon National University Department of Physiology College of MedicineInstitute of Neurodegeneration and NeuroregenerationHallym University Department of Anatomy and Cell Biology College of Veterinary MedicineSeoul National University Department of Emergency Medicine School of MedicineKangwon National University Department of Food Science and Nutrition Hallym University

Long-term administration of scopolamine, a muscarinic receptor antagonist, can inhibit the survival of newly generated cells, but its effect on the proliferation, differentiation and migration of nerve cells in the adult mouse hippocampal dentate gyrus remain poorly understood. In this study, we used immunohistochemistry and western blot methods to weekly detect the biological behaviors of nerve cells in the hippocampal dentate gyrus of adult mice that received intraperito- neal administration of scopolamine for 4 weeks. Expression of neuronal nuclear antigen （NeuN; a neuronal marker） and Fluoro-]ade B （a marker for the localization of neuronal degeneration） was also detected. After scopolamine treatment, mouse hippocampal neurons did not die, and Ki-67 （a marker for proliferating cells）-immunoreactive cells were reduced in number and reac hed the lowest level at 4 weeks. Doublecortin （DCX; a marker for newly generated neurons）-im- munoreactive cells were gradually shortened in length and reduced in number with time. After scopolamine treatment for 4 weeks, nearly all of the 5-bromo-2＇-deoxyuridine （BrdU）-labeled newly generated cells were located in the subgranular zone of the dentate gyrus, but they did not migrate into the granule cell layer. Few mature BrdU/NeuN double-labeled cells were seen in the subgranular zone of the dentate gyrus. These findings suggest that long-term administration of scopolamine interferes with the proliferation, differentiation and migration of nerve cells in the adult mouse hippocampal dentate gyrus, but it does not induce cell death.

关键词： nerve regeneration neurogenesis scopolamine dentate gyrus cell proliferation neuroblastdifferentiation neuroblast migration granule cell layer neural regeneration

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Autonomous self-healing 3D micro-suction adhesives for multi-layered amphibious soft skin electronics

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InfoMat 2024年第10期6卷 65-79页

作者： Dohyun Lim Min Woo Jeong Hyeongho Min Yeon Soo Lee Gui Won hwang Seung Hwan Jeon Kyu ho Jung Ngoc Thanh Phuong Vo Min-Seok Kim Da Wan Kim Jin Young Oh Changhyun Pang School of Chemical Engineering Sungkyunkwan University(SKKU)SuwonGyeonggi-doRepublic of Korea Department of Chemical Engineering(Integrated Engineering Program) Kyung Hee UniversityYonginGyeonggiRepublic of Korea Convergence Research Group for Meta-Touch Korea Research Institute of Standards and ScienceDaejeonRepublic of Korea Department of Electronic Engineering Korea National University of TransportationChungju-siChungbukRepublic of Korea Samsung Advanced Institute for Health Sciences and Technology(SAIHST) Sungkyunkwan UniversitySuwonGyunggi-doRepublic of Korea

Autonomously self-healing, reversible, and soft adhesive microarchitecturesand structured electric elements could be important features in stable and versatilebioelectronic devices adhere to complex surfaces of the human body(rough, dry, wet, and vulnerable). In this study, we propose an autonomousself-healing multi-layered adhesive patch inspired by the octopus, which possessself-healing and robust adhesion properties in dry/underwater *** implement autonomously self-healing octopus-inspired architectures, adynamic polymer reflow model based on structural and material design suggestscriteria for three-dimensional patterning self-healing elastomers. In addition,self-healing multi-layered microstructures with different moduli endowsefficient self-healing ability, human-friendly reversible bio-adhesion, and stablemechanical deformability. Through programmed molecular behavior ofmicrolevel hybrid multiscale architectures, the bioinspired adhesive patchexhibited robust adhesion against rough skin surface under both dry andunderwater conditions while enabling autonomous adhesion restoring performanceafter damaged (over 95% healing efficiency under both conditions for24 h at 30℃). Finally, we developed a self-healing skin-mountable adhesiveelectronics with repeated attachment and minimal skin irritation by laminatingthin gold electrodes on octopus-like structures. Based on the robust adhesionand intimate contact with skin, we successfully obtained reliable measurements during dynamic motion under dry, wet, and damagedconditions.

关键词： biomimetics dry adhesive self-healing polymer stretchable electronics

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Emerging and re-emerging infectious diseases: Challenges and opportunities for militaries

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Journal of Medical Colleges of PLA(China) 2014年第2期29卷 125-136页

作者： Zheng Jie Marc ho Yi Fu Jeff hwang Jian Ming Vernon Lee Biodefence Centre Headquarters of the Medical CorpsSingapore Armed Forces701 Transit Road#04-01Singapore 778910Singapore

The communal nature of living and training environments, alongside suboptimal hygiene and stressors in the field, place military personnel at higher risk of contracting emerging infectious diseases. Some of these diseases spread quickly within ranks resulting in large outbreaks, and personnel deployed are also often immunologically na?ve to otherwise uncommonly-encountered pathogens. Furthermore, the chance of weaponised biological agents being used in conventional warfare or otherwise remains a very real, albeit often veiled, threat. however, such challenges also provide opportunities for the advancement of preventive and therapeutic military medicine, some of which have been later adopted in civilian settings. Some of these include improved surveillance, new vaccines and drugs, better public health interventions and inter-agency co-operations. The legacy of successes in dealing with infectious diseases is a reminder of the importance in sustaining efforts aimed at ensuring a safer environment for both military and the community at large.

关键词： communicable diseases emerging infectious disease medicine military personnel

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