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hla-DRB13*11阳性患者较早发生严重斑秃

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世界核心医学期刊文摘（皮肤病学分册） 2006年第9期2卷 46-47页

作者： marques Da Costa C. Dupont E Van Der Cruys m. 张路坤 Department of Immunology-Hematology-Transfusion Erasme Hospital 808 Route de Lennik BE- 1070 Brussels Belgium Dr.

Background: Alopecia areata (AA) is a polygenic immunemediated disorder affecting the hair follicle for which an association with human leukocyte antigen hla-DRB1 11 has been described. Objective: Two parameters including age of onset and extent of the disease (patchy AA and AT/AU forms)- were correlatedwith the presence or absence of hla-DRB1 11 and its alleles in 88 severe AA patients. methods: Patients and healthy controls were typed for hla-DR and - DQ by molecular method. Results: Among AA patients, 37.5% (a proportion rising to 72% when taking patients who began their first patch before the age of 20 years) were positive for hlaDRB1 11 compared to 21.2% healthy controls (p = 0.004, RR = 2.1). DRB1 11-positive status was associated with earlier development of the first AA patch, at the mean age of 16 years compared to 27 years (p = 0.003) in DRB1 11-negative patients. Among the DRB1 11 alleles, the presence of DRB1 1104was associated with the earliest occurrence of AA. Conclusion: Our data indicate that the hla system largely through DRB1 1104 allele influences AA onset rather than extension considering patchy AA and AT/AU.

关键词：阳性患者斑秃 hla-DQ 健康对照组人类白细胞抗原分子生物学方法发病年龄早发

Contribution of functional KIR3DL1 to ankylosing spondylitis

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Cellular & molecular Immunology 2010年第6期7卷 471-476页

作者： Ivan V Zvyagin Ilgar Z mamedov Olga V Britanova Dmitriy B Staroverov Evgeni L Nasonov Anna G Bochkova Anna V Chkalina Alexei A Kotlobay Dmitriy O Korostin Denis V Rebrikov Sergey Lukyanov Yuri B Lebedev Dmitriy m Chudakov Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry RASMoscowRussia Institute of Rheumatology RAMSMoscowRussia DNA-Technology JSC MoscowRussia Vavilov Institute of General Genetics MoscowRussia

Increasing evidence points to a role for killer immunoglobulin-like receptors(KIRs)in the development of autoimmune *** particular,a positive association of KIR3DS1(activating receptor)and a negative association of KIR3DL1(inhibitory receptor)alleles with ankylosing spondylitis(AS)have been reported by several ***,none of the studies analyzed these associations in the context of functionality of polymorphic *** better understand how the KIR3DL1/3DS1 genes determine susceptibility to AS,we analyzed the frequencies of alleles and genotypes encoding functional(KIR3DL1*F)and non-functional(KIR3DL1*004)*** genotyped 83 AS patients and 107 human leukocyte antigen(hla)-B27-positive healthy controls from the Russian Caucasian population using a two-stage sequence-specific primer PCR,which distinguishes KIR3DS1,KIR3DL1*F and KIR3DL1*004 *** the patients carrying two functional KIR3DL1 alleles,those alleles were additionally genotyped to identify KIR3DL1*005 and KIR3DL1*007 alleles,which are functional but are expressed at low ***3DL1 was negatively associated with AS at the expense of KIR3DL1*F but not of KIR3DL1**** finding indicates that the inhibitory KIR3DL1 receptor protects against the development of AS and is not simply a passive counterpart of the segregating KIR3DS1 allele encoding the activating ***,analysis of genotype frequencies indicates that the presence of KIR3DS1 is a more important factor for AS susceptibility than the absence of KIR3DL1**** activation of either natural killer(NK)or T cells via the KIR3DS1 receptor can be one of the critical events in AS development,while the presence of the functional KIR3DL1 receptor has a protective ***,even individuals with a genotype that carried two inhibitory KIR3DL1 alleles expressed at high levels could develop AS.

关键词： ankylosing spondylitis autoimmune disease hla-B27 KIR KIR3DL1 KIR3DS1 KIR3DL1*004 NK cell T cell

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强直性脊柱炎的诊断和治疗

英国医学杂志中文版 2008年第3期11卷 154-158页

作者： Claire m mcVeigh Andrew P Cairns 牛素平(译) 赵龠陶(译) 李圣楠(译) 黄慈波(校) Department of Rheumatology Musgrave Park Hospital Belfast BT9 7JB 不详

强直性脊柱炎是一种慢性炎性风湿病，主要影响骶髂关节、脊柱和附着点与人白细胞抗原（hla）B27有很强的遗传关联早期诊断比较困难但很重要；骶髂关节的磁共振成像对于早期诊断很有价值传统治疗包括物理治疗、规律使用非甾体类抗炎药、... 详细信息

强直性脊柱炎是一种慢性炎性风湿病，主要影响骶髂关节、脊柱和附着点与人白细胞抗原（hla）B27有很强的遗传关联早期诊断比较困难但很重要；骶髂关节的磁共振成像对于早期诊断很有价值传统治疗包括物理治疗、规律使用非甾体类抗炎药、局部皮质激素注射，或者有时使用柳氮磺胺吡啶肿瘤坏死因子α（TNF-α）抑制剂的出现在治疗上是一个突破炎性腰背痛是病人的特征表现。

关键词：强直性脊柱炎物理治疗诊断比较非甾体类抗炎药肿瘤坏死因子α 人白细胞抗原早期诊断柳氮磺胺吡啶

Identification of an hla-A^＊ 0201-restricted CD^8＋ T-cell epitope SSp-1 of SARS-CoV spike protein

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第二军医大学学报 2005年第11期26卷 1269-1269页

作者： Wang B Chen H Jiang X Zhang m Wan T Li N Zhou X Wu Y Yang F Yu Y Wang X Yang R Cao X Institute of Immunology Second Military Medical University Shanghai 200433 China

胎盘免疫调节因子对细胞因子诱导的杀伤细胞超微结构及免疫表型的调节作用

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中国组织工程研究与临床康复 2007年第23期11卷 4461-4465页

作者：张学荣蒙怡舒雨雁韦敏石庆秋徐阳曦广西医科大学医学科学实验中心蛇毒研究所广西壮族自治区南宁市530021

目的:通过电子显微镜、流式细胞仪观察经胎盘免疫调节因子处理的细胞因子诱导的杀伤细胞超微结构及免疫表型变化,了解胎盘免疫调节因子的免疫调节活性。方法:实验于2006-02/2007-01在广西医科大学医学科学实验中心重点实验室进行。实验... 详细信息

目的:通过电子显微镜、流式细胞仪观察经胎盘免疫调节因子处理的细胞因子诱导的杀伤细胞超微结构及免疫表型变化,了解胎盘免疫调节因子的免疫调节活性。方法:实验于2006-02/2007-01在广西医科大学医学科学实验中心重点实验室进行。实验材料:重组人干扰素γ和白细胞介素1α为Peprotech公司产品。重组人白细胞介素2和抗人CD3单克隆抗体为北京远策药业有限公司产品。实验方法:①胎盘免疫调节因子的制备:将新鲜经检人胎盘去筋膜,生理盐水冲净、剪碎匀浆,置-20℃48h后,反复冻融3次,用生理盐水透析,取透析外液,过虑除菌,分装,抽样做有关鉴定实验。②细胞因子诱导的杀伤细胞的制备:抽取健康自愿者静脉血20mL,用淋巴细胞分离液分离外周血单个核细胞,加RPmI1640培养液调整细胞的密度至1×109L-1,放于50mL培养瓶中培养。于当天加入1×106U/L干扰素γ,置于37℃、50mL/LCO2孵育箱中培养24h。次日将悬浮的细胞转移到6孔板培养中,分别加入终浓度为100mg/L的PHA、3×105U/L白细胞介素2、133μg/L抗CD3mAb及1×105U/L白细胞介素1α,每3d半量换液1次,同时补加白细胞介素2至3×105U/L。将培养14d细胞因子诱导的杀伤细胞以5×109L-1接种于24孔板,实验组中加入一定剂量的胎盘免疫调节因子诱导,对照组加入等量生理盐水,继续培养72h。③实验评估:制备电镜标本,观察细胞超微结构。采用流式细胞术测定细胞因子诱导的杀伤细胞CD4+、CD8+、CD3+CD4+、CD3+CD8+、CD25+、hla-DR、CD3+CD56+、CD80+百分含量。结果:①细胞因子诱导的杀伤细胞表型分析:实验组CD4+、CD8+和CD3+CD4+明显低于对照组[实验组:(8.40±8.15)%,(33.10±4.61)%,(7.98±12.65%);对照组:(39.43±9.16)%,(58.90±11.35)%,(31.30±5.94)%,P<0.05];实验组CD3+CD8+、CD25+表达明显高于对照组[实验组:(30.50±2.56)%,(24.8±6.31)%;对照组:(15.90±7.35)%,(0.56±0.21)%,P<0.05]。②透视电镜下观察细胞因子诱导的杀伤细胞超微结构特征:与对照组相比,实验组细胞因子诱导的杀伤细胞线粒体深染,溶酶体增加,异染色质比例增加,微绒毛肿胀及增长等。结论:胎盘免疫调节因子能促使细胞因子诱导的杀伤细胞超微结构及细胞表型发生明显变化:细胞因子诱导的杀伤细胞线粒体深染,溶酶体增加,异染色质比例增加,微绒毛肿胀增长,CD3+CD8+、CD25+细胞百分比升高,CD4+、CD8+和CD3+CD4+细胞百分比降低。

关键词：胎盘免疫调节因子 CIK细胞超微结构细胞表型

小胶质细胞在人原发性视网膜色素变性中的活化

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眼科研究 2008年第2期26卷 129-132页

作者：曾惠阳曹安民朱秀安北京大学第三医院北京大学眼科中心 100083 美国约翰·霍普金斯大学威尔玛眼科研究所马里兰州巴尔的摩市21287

目的观察小胶质细胞在原发性视网膜色素变性(RP)患者中的活化表现。方法制备22个原发性RP患者及18个正常人眼的石蜡切片。抗体hla-DR,CD45及CD68对视网膜小胶质细胞进行免疫标记。结果RP患者赤道部视网膜内层出现大量肥大的活化小胶质细... 详细信息

目的观察小胶质细胞在原发性视网膜色素变性(RP)患者中的活化表现。方法制备22个原发性RP患者及18个正常人眼的石蜡切片。抗体hla-DR,CD45及CD68对视网膜小胶质细胞进行免疫标记。结果RP患者赤道部视网膜内层出现大量肥大的活化小胶质细胞,部分向变性的感光细胞层浸润。黄斑区小胶质细胞主要以三种形式存在:在视网膜内层明显活化并向感光细胞层浸润;在视网膜内层活化但很少向外核层浸润;视网膜全层未见小胶质细胞活化。视神经及视网膜前膜均见小胶质细胞活化。结论小胶质细胞的活化是RP的一种独特的炎性反应方式,在感光细胞变性中发挥一定作用。

关键词：小胶质细胞原发性视网膜色素变性视网膜

Genetic barriers in transplantation medicine

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World Journal of Transplantation 2016年第3期6卷 532-541页

作者： Hisham A Edinur Siti m manaf Nor F Che mat Forensic Science Programme School of Health Sciences Universiti Sains Malaysia Biomedicine Programme School of Health Sciences Universiti Sains Malaysia

The successful of transplantation is determined by the shared human leukocyte antigens(hlas) and ABO blood group antigens between donor and recipient. In recent years, killer cell receptor [i.e., killer cell immunoglobulinlike receptor(KIR)] and major histocompatibility complex(mHC) class I chain-related gene molecule(i.e., mICA) were also reported as important determinants of transplant compatibility. At present, several different genotyping techniques(e.g., sequence specific primer and sequence based typing) can be used to characterize blood group, hla, mICA and KIR and loci. These molecular techniques have several advantages because they do not depend on the availability of anti-sera, cellular expression and have greater specificity and accuracy compared with the antibody-antigen based typing. Nonetheless, these molecular techniques have limited capability to capture increasing number of markers which have been demonstrated to determine donor and recipient compatibility. It is now possible to genotype multiple markers and to the extent of a complete sequencing of the human genome using next generation sequencer(NGS). This high throughput genotyping platform has been tested for hla, and it is expected that NGS will be used to simultaneously genotype a large number of clinically relevant transplantation genes in near future. This is not far from reality due to the bioinformatics support given by the immunogenetics community and the rigorous improvement in NGS methodology. In addition, new developments in immune tolerance based therapy, donor recruitment strategies and bioengineering are expected to provide significant advances in the field of transplantation medicine.

关键词： Transplantation ABO blood group Human leukocyte antigen mICA Killer cell immunoglobulin-like receptor Graft rejection Graft vs host disease

Interest of Beta-2-microglobilin Plasma Assay in Iterative Haemodialysis in the Nephrology and Haemodialysis Department of University Teaching Hospital of Point G, Bamako, mali

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Open Journal of Nephrology 2020年第4期10卷 282-289页

作者： Saharé Fongoro magara Samaké Seydou Sy Hamadoun Yattara Djénèba Diallo Jacques Coulibaly moctar Coulibaly Aboubacar Sidiki Fofana Atabième Kodio Djénéba maiga Aboudou m. Dolo moustapha Tangara Nouhoum Coulibaly Brahima Dégoga Ibrahima Koné Nephrology and Haemodialysis Department of the University Teaching Hospital of Point-G Bamako Mali Faculty of medicine of Bamako Bamako Mali Nephrology Unit of the Fousseyni DAOU Hospital in Kayes Kayes Mali Nephrology Unit of Ségou Hospital Ségou Mali Nephrology Unit of the mali GAVARDO Hospital of Sébénicoro Bamako Mali Nephrology Unit of Sikasso Hospital Sikasso Mali Nephrology Unit of Somino DOLO Hospital in mopti Mopti Mali

Beta-2-microglobulin (B2m) is a low molecular weight polypeptide (11,800 Da) that exists in a free form and a form bound to cell membranes (light chains of hla class I molecules). Their dosage is used in the exploration and monitoring of renal function in haemodialysis patients, hence the interest of this study, the objective of which is to study the different players involved in the increase of beta-2-microglobulin. materials and methods: This was a prospective and descriptive study from January 1st, 2017 to August 31, 2017 (08 months). All patients with chronic hemodialysis for 3 years or more were included. We analyzed sociodemographic, clinical and paraclinical data. Results: We collected 50 patients. The mean age was 50 ± 4.6 years with extremes of 30 and 82 years. The female sex predominated (52%). Chronic end-stage renal failure was hypertensive (86%), diabetic (4%). All patients benefited from intermittent hemodialysis for 8 hours per week using a low permeability membrane (cuprophane). The average duration of hemodialysis was 68 ± 6.4 months with extremes of 36 and 204 months. Twenty percent (20%) and four percent (4%) of chronic dialysis patients had joint pain and sensory deficit. Phalen’s sign was positive in 2% (1 case), Tinel’s sign in 2% (1 case) and carpal tunnel syndrome in 2% (1 case). The mean serum beta-2-microglobulin level was 58.34 ng/l with extremes of 16.99 and 112.24 ng/l. There was a correlation between hypertensive nephropathies and beta-2-microglobulin levels above 50 ng/l (P mortality rate was 6%. Conclusion: Our study has objectified factors such as inadequate dialysis (8 hours/week), use of low permeability membrane in the increase of beta-2-microglobulin. The increase in serum beta-2-microglobulin levels in our patients is evidence of poor purification of medium molecules. Its reduction can be established by improving the quality of dialysis, in particular by

关键词： Chronic Hemodialysis Beta-2-microglobulin Carpal Tunnel Syndrome

西双版纳傣族及上海汉族群体hla－DR2相关的DR／DQ单倍型分析

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中华医学遗传学杂志 1995年第3期12卷 134-137页

作者：范丽安杨珏琴姚芳娟董建中许玲娣陈仁彪 m Sirkong R Vongrathna P Longta S Lekmak D Chandanayingyong A Smith J Hansen 上海第二医科大学、上海免疫学研究所免疫遗传学研究室上海第二医科大学、上海免疫学研究所医学遗传教研室泰国马海同大学美国弗莱特赫钦森癌症研究中心

对４４名西双版纳傣族和９名上海地区汉族ＤＫ２阳性个体进行了与其相关的ＤＲ／ＤＱ单倍型组合的分析。傣族群体中ＤＲＢＩ－ＤＲ２亚型分布以＊１６０２与＊１５０２为最常见，其等位基因频率分别为４３．６％与，４０．０％和汉... 详细信息

对４４名西双版纳傣族和９名上海地区汉族ＤＫ２阳性个体进行了与其相关的ＤＲ／ＤＱ单倍型组合的分析。傣族群体中ＤＲＢＩ－ＤＲ２亚型分布以＊１６０２与＊１５０２为最常见，其等位基因频率分别为４３．６％与，４０．０％和汉族群体中以＊１５０１为主明显不同。傣族群体中共检出１０种与ＤＲ２相关联的ＤＲ／ＤＱ单倍型；最常见的是ＤＲＢ１＊１６０２、ＤＲＢ５＊０１０１、ＤＱＡ１＊０１０２、ＤＱＢ１＊０５０２（３４．５％）与汉族及其他群体明显不同，本研究表明傣族不仅具有高频率的ＤＲ２，而且与ＤＲ２相关联的ＤＲＢ１、ＤＲＢ５、ＤＱＡ１、ＤＱＢ１单倍型组合有其独特性。

关键词：傣族汉族 DR2-单倍型 DRB1*1504 人类白细胞抗原