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Fabrication of plasmonic nanopyramidal array as flexible SERS substrate for biosensing application

Nano Research 2023年第1期16卷 1132-1140页

作者： Anindita Das Udit Pant Cuong Cao Rakesh SMoirangthem Hitesh Bhanudas Kamble Nanophotonics Lab Department of PhysicsIndian Institute of Technology(Indian School of Mines)Dhanbad-826004India Institute for Global Food Security School of Biological SciencesQueen’s University of Belfast19 Chlorine GardensBelfastBT95DLUK Material and Advanced Technologies for Healthcare Queen’s University of Belfast18-30 Malone RoadBelfastBT95BNUK IITB Nanofabrication Facility Department of Electrical EngineeringIndian Institute of TechnologyBombay-400076India

The proposed work aims to develop a sensitive surface-enhanced Raman spectroscopy(SERS)nano-biosensor.The inverted nano pyramid array on silicon substrate fabricated using electron beam lithography(EBL)was utilised as a master template and the mold was later replicated via nanoimprinting process to prepare gold-coated polymer nanopyramid three-dimensional(3D)SERS substrate.The fast and versatile replication process using nanoimprinting lithography(NIL)can produce polymer nanopyramids in a low-cost and reproducible fashion.Also,the proposed fabrication protocol can be easily upscale for large scale fabrication.The intense electric field confinement at nanotips and four edges of gold-coated polymer nanopyramid enhanced the Raman signal of probe molecules,i.e.,Rhodamine 6G with a limit of detection down to 3.277×10−9 M was achieved.This work also underlines the efficiency of gold-coated polymer nanopyramid arrays in the spectral detection of hemoglobin proteins at low concentrations.The Raman signal enhancement mechanism was further studied through the electromagnetic simulation using COMSOL Multiphysics.In addition,bending test experiments were performed to understand the effect of flexibility on SERS signal response.The fabricated gold-coated polymer nanopyramids arrays could pave the way for the development of low-cost SERS platforms for the detection of hazardous biological and chemical compounds at ultra-low concentrations in practical applications.

关键词： nanopyramids surface-enhanced Raman spectroscopy(SERS) flexible hemoglobin protein nanoimprinting replica molding

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