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Epithelial cell adhesion efficacy of a novel peptide identified by panning on a smooth titanium surface

International Journal of Oral Science 2018年第3期10卷 174-181页

作者： Hidemichi Kihara David M.Kim Masazumi Nagai Toshiki Nojiri Shigemi Nagai Chia-Yu Chen Cliff Lee Wataru Hatakeyama hisatomo kondo John Da Silva Harvard School of Dental Medicine BostonMAUSA School of Dental Medicine Iwate Medical UniversityIwateJapan University of California San FranciscoCAUSA

Epithelial attachment via the basal lamina on the tooth surface provides an important structural defence mechanism against bacterial invasion in combating periodontal disease. However, when considering dental implants, strong epithelial attachment does not exist throughout the titanium-soft tissue interface, making soft tissues more susceptible to peri-implant disease. This study introduced a novel synthetic peptide(A10) to enhance epithelial attachment. A10 was identified from a bacterial peptide display library and synthesized. A10 and protease-activated receptor 4-activating peptide(PAR4-AP, positive control) were immobilized on commercially pure titanium. The peptide-treated titanium showed high epithelial cell migration ability during incubation in platelet-rich plasma. We confirmed the development of dense and expanded BL(stained by Ln5) with pericellular junctions(stained by ZO1) on the peptide-treated titanium surface. In an adhesion assay of epithelial cells on A10-treated titanium, PAR4-AP-treated titanium, bovine root and non-treated titanium, A10-treated titanium and PAR4-AP-treated titanium showed significantly stronger adhesion than non-treated titanium. PAR4-AP-treated titanium showed significantly higher inflammatory cytokine release than non-treated titanium. There was no significant difference in inflammatory cytokine release between A10-treated and non-treated titanium. These results indicated that A10 could induce the adhesion and migration of epithelial cells with low inflammatory cytokine release. This novel peptide has a potentially useful application that could improve clinical outcomes with titanium implants and abutments by reducing or preventing peri-implant disease.

关键词： PAR OBA Epithelial cell adhesion efficacy of a novel peptide identified by panning on a smooth titanium surface AP

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Clinical outcomes and risk factors for perforation in gastric endoscopic submucosal dissection: A prospective pilot study

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World Journal of Gastrointestinal Endoscopy 2013年第6期5卷 281-287页

作者： Jiro Watari Toshihiko Tomita Fumihiko Toyoshima Jun Sakurai Takashi kondo Haruki Asano Takahisa Yamasaki Takuya Okugawa hisatomo Ikehara Tadayuki Oshima Hirokazu Fukui Hiroto Miwa Division of Upper Gastroenterology Department of Internal Medicine Hyogo College of Medicine

AIM: To evaluate clinical outcomes and risk factors for endoscopic perforation during endoscopic submucosal dissection (ESD) in a prospective study. METHODS: We investigated the clinical outcomes and risk factors for the development of perforation in 98 consecutive gastric neoplasms undergoing ESD regarding. Demographic and clinical parameters including patient-, tumor-, and treatment-related factors, clinical parameters, and duration of hospital stay were analyzed for risk factors for perforation. In subgroup analysis, we also compared the clinical outcomes between perforation and "silent" free air without endoscopically visible perforation detected only by computed ***: Perforation was identified in 8.2% of patients. All patients were managed conservatively by the administration of antibiotics. The mean procedure time was significantly longer in patients with endoscopic perforation than in those without. According to the receiver-operating characteristic analysis, the resulting cutoff value of the procedure time for perforation was 115 min (87.5% sensitivity, 56.7% specificity). Prolonged procedure time (≥ 115 min) was associated with an increased risk of perforation (odds ratio 9.15; 95%CI: 1.08-77.54; P = 0.04). Following ESD, body temperature and C-reactive protein level were significantly higher in patients with perforation than in those without (P = 0.02), whereas there was no difference between these patient groups on the starting day of oral intake or of hospitalization. In subgroup analysis, the post-ESD clinical course was not different between endoscopic perforation and silent free air. CONCLUSION: Only prolonged procedure time (≥ 115 min) was significantly associated with perforation. The clinical outcomes of perforation are favorable and are comparable to those of patients with or without silent free air.

关键词： Gastric cancer Endoscopic submucosal dissection Perforation Risk factors Treatment outcome

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What types of early gastric cancer are indicated for endoscopic ultrasonography staging of invasion depth?

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World Journal of Gastrointestinal Endoscopy 2016年第16期8卷 558-567页

作者： Jiro Watari Shigemitsu Ueyama Toshihiko Tomita hisatomo Ikehara Kazutoshi Hori Ken Hara Takahisa Yamasaki Takuya Okugawa Takashi kondo Tomoaki Kono Katsuyuki Tozawa Tadayuki Oshima Hirokazu Fukui Hiroto Miwa Division of Gastroenterology Department of Internal Medicine Hyogo College of Medicine Department of Inflammatory Bowel Disease Hyogo College of Medicine

AIM To clarify the diagnostic efficacy and limitations of endoscopic ultrasonography(EUS) and the characteristics of early gastric cancers(EGCs) that are indications for EUS-based assessment of cancer invasion *** We retrospectively investigated the cases of 153 EGC patients who underwent conventional endoscopy(CE) and EUS(20 MHz) before *** We found that 13.7% were "inconclusive" cases with low-quality EUS images, including all nine of the cases with protruded(0-I)-type EGCs. There was no significant difference in the diagnostic accuracybetween CE and EUS. Two significant independent risk factors for misdiagnosis by EUS were identified-ulcer scarring [UL(+); odds ratio(OR) = 4.49, P = 0.003] and non-indication criteria for endoscopic resection(ER)(OR = 3.02, P = 0.03). In the subgroup analysis, 23.1% of the differentiated-type cancers exhibiting SM massive invasion(SM2) invasion(submucosal invasion ≥ 500 μm) by CE were correctly diagnosed by EUS, and 23.1% of the undifferentiated-type EGCs meeting the expanded-indication criteria for ER were correctly diagnosed by *** There is no need to perform EUS for UL(+) EGCs or 0-I-type EGCs, but EUS may enhance the pretreatment staging of differentiated-type EGCs with SM2 invasion without UL or undifferentiated-type EGCs revealed by CE as meeting the expanded-indication criteria for ER.

关键词： Gastric cancer Endoscopic ultrasonography Invasion depth diagnosis Conventional endoscopy Endoscopic submucosal dissection

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Histological diagnosis of gastric submucosal tumors: A pilot study of endoscopic ultrasonography-guided fine-needle aspiration biopsy vs mucosal cutting biopsy

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World Journal of Gastrointestinal Endoscopy 2015年第14期7卷 1142-1149页

作者： hisatomo Ikehara Zhaoliang Li Jiro Watari Masato Taki Tomohiro Ogawa Takahisa Yamasaki Takashi kondo Fumihiko Toyoshima Tomoaki Kono Katsuyuki Tozawa Yoshio Ohda Toshihiko Tomita Tadayuki Oshima Hirokazu Fukui Ikuo Matsuda Seiichi Hirota Hiroto Miwa Division of Gastroenterology Department of Internal Medicine Hyogo College of Medicine Department of Surgical Pathology Hyogo College of Medicine

AIM: To compare the usefulness of endoscopic ultrasonography-guided fine-needle aspiration biopsy(EUS-FNAB) without cytology and mucosal cutting biopsy(MCB) in the histological diagnosis of gastric submucosal tumor(SMT).METHODS: We prospectively compared the diagnostic yield, feasibility, and safety of EUS-FNAB and those of MCB based on endoscopic submucosal dissection. The cases of 20 consecutive patients with gastric SMT ≥1 cm in diameter. who underwent both EUS-FNAB and MCB were ***: The histological diagnoses were gastrointestinal stromal tumors(n = 7), leiomyoma(n =6), schwannoma(n = 2), aberrant pancreas(n = 2), and one case each of glomus tumor, metastatic hepatocellular carcinoma, and no-diagnosis. The tumors' mean size was 23.6 mm. Histological diagnosis was made in 65.0% of the EUS-FNABs and 60.0% of the MCBs, a nonsignificant difference. There were no significant differences in the diagnostic yield concerning the tumor location or tumor size between the two methods. However, diagnostic specimens were significantly more frequently obtained in lesions with intraluminal growth than in those with extraluminal growth by the MCB method(P = 0.01). All four SMTs with extraluminal growth were diagnosed only by EUSFNAB(P = 0.03). No complications were found in either ***: MCB may be chosen as an alternative diagnostic modality in tumors showing the intraluminal growth pattern regardless of tumor size, whereas EUSFNAB should be performed for SMTs with extraluminal growth.

关键词： Submucosal tumor Endoscopic ultrasonography-guided

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Diagnosis of small intramucosal signet ring cell carcinoma of the stomach by non-magnifying narrow-band imaging: A pilot study

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World Journal of Gastrointestinal Endoscopy 2015年第12期7卷 1070-1077页

作者： Jiro Watari Toshihiko Tomita hisatomo Ikehara Masato Taki Tomohiro Ogawa Takahisa Yamasaki Takashi kondo Fumihiko Toyoshima Jun Sakurai Tomoaki Kono Katsuyuki Tozawa Yoshio Ohda Tadayuki Oshima Hirokazu Fukui Seiichi Hirota Hiroto Miwa Division of Gastroenterology Department of Internal Medicine Hyogo College of Medicine NishinomiyaHyogo 663-8501 Japan Department of Surgical Pathology Hyogo College of Medicine Nishinomiya Hyogo 663-8501 Japan Division of Gastroenterology Department of Internal Medicine Hyogo College of MedicineNishinomiyaHyogo 663-8501 Japan

AIM: To examine the efficacy of non-magnifying narrow-band imaging(NM-NBI) imaging for small signet ring cell carcinoma(SRC).METHODS: We retrospectively analyzed 14 consecutive small intramucosal SRCs that had been treated with endoscopic submucosal dissection(ESD) and 14 randomly selected whitish gastric ulcer scars(control). The strength and shape of the SRCs and whitish scars by NM-NBI and white-light imaging(WLI) were assessed with Image J(NIH, Bethesda).RESULTS: NM-NBI findings of SRC showed a clearly isolated whitish area amid the brown color of the surrounding normal mucosa. The NBI index, which indicates the potency of NBI for visualizing SRC, was significantly higher than the WLI index(P = 0.001), indicating SRC was more clearly identified by NM-NBI. Although the NBI index was not significantly different between SRCs and controls, the circle(C)-index, as an index of circularity of tumor shape, was significantly higher in SRCs(P = 0.001). According to the receiveroperating characteristic analysis, the resulting cut-off value of the circularity index(C-index) for SRC was 0.60(85.7% sensitivity, 85.7% specificity). Thus a lesion with a C-index ≥ 0.6 was significantly more likely to be an SRC than a gastric ulcer scar(OR = 36.0; 95%CI: 4.33-299.09; P = 0.0009).CONCLUSION: Small isolated whitish round area by NM-NBI endoscopy is a useful finding of SRCs which is the indication for ESD.

关键词： Gastric cancer Signet ring cell carcinoma Narrow-b

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