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欠定和不确定条件下胞内代谢通量的最大熵原理估算

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生物工程学报 2008年第12期24卷 2135-2136页

作者： Quanyu Zhao hiroyuki kurata Department of Bioscience and Bioinformatics Kyushu Institute of Technology 680-4 Kawazu Iizuka Fukuoka 820-8502 Japan

在代谢工程和系统生物学领域,计算机模拟比以往更为有效的应用于生物过程的分析和优化。胞内代谢通量可以用代谢通量分析和基元模式分析来估算。由于测定数据的不足和误差,以及基元途径的冗余,经常很难得到准确的代谢通量分布数据。本... 详细信息

在代谢工程和系统生物学领域,计算机模拟比以往更为有效的应用于生物过程的分析和优化。胞内代谢通量可以用代谢通量分析和基元模式分析来估算。由于测定数据的不足和误差,以及基元途径的冗余,经常很难得到准确的代谢通量分布数据。本研究提出一种基于最大熵原理的算法来计算基元模式系数。欠定和不确定条件下,通过胞外代谢通量数据估算胞内代谢通量分布。为了检验算法的可行性,对杂交瘤细胞、枯草芽孢杆菌和大肠杆菌的胞内代谢通量分布做了估算。本研究提出的基于最大熵原理的优化算法避免了对细胞状态的生理学假设。与其他目标函数相比,可以更为可靠和可行的估算胞内代谢通量分布。

关键词：基元模式酶控制通量最大熵原理代谢通量分析

Effect of cra gene mutation on the metabolism of <i>Escherichia coli</i>for a mixture of multiple carbon sources

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Advances in Bioscience and Biotechnology 2013年第3期4卷 477-486页

作者： Ruilian Yao hiroyuki kurata Kazuyuki Shimizu Department of Bioscience and Bioinformatics Kyushu Institute of Technology Iizuka Japan Institute of Advanced Bioscience Keio University Tsuruoka Japan

The major player for catabolite repression is the phosphotransferase systems (PTSs) and cAMP-Crp. Moreover, Cra controls the carbon flow in the metabolic network. In the present research, the effect of modulating cra gene (Δcra) on the consumption of multiple carbon sources such as glucose and fructose (as well as xylose) was investigated under both aerobic and anaerobic conditions. It was shown that glucose and fructose could be co-metabolized with fructose consumed faster than glucose in cra mutant under both aerobic and anaerobic conditions. It was also implied that cra mutant consumed higher amount of total carbon sources, which contributed to the highest metabolite production as compared to the wild type strain. Thus, cra mutant can be a good candidate for the efficient utilization of multiple carbon sources such as glucose and fructose, where xylose consumption was repressed by catabolite repression. The overall regulation mechanisms were clarified based on fermentation data and gene transcript analysis.

关键词： Escherichia coli cra Mutant Catabolite Regulation

APPLICABILITY OF CREEP CONSTITUTIVE EQUATIONS TOCREEP CURVES UNDER CONSTANT LOAD

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Acta Metallurgica Sinica(English Letters) 1998年第6期11卷 397-404页

作者： Y.kurata H.Utsumi Japan Atomic Energy Research Inst Ibaraki-ken Japan

Applicability of the following creep constitutive equations was investigated for normal type creep curves of Ni-18.5Cr alloy and tertiary creep dominant curves of Ni-18.5Cr16W alloy under constant load: the θ projection method describing a creep curse by a sum of two exponential terms, modified θ method describing a primary creep stage by an exponential term and a tertiary creep stage by a logarithmic term, modified Ω method describing a creep curve by a sum of two logarithmic term, 2θ method with only a tertiary creep component and Ω method. The θ, modified θ and modified Ω methods can describe normal type and tertiary creep dominant curves. Tertiary creep dominant curves of Ni-18.5Cr-16W alloy at 900℃ are also described using 2θ and Ω methods. Applicability of the modified θ and modified Ω methods is superior for constant load creep curves because they can predict creep curves up to rupture and rupture life accurately and conservatively.

关键词： creep equation θ projection method Ω method Ni-18.5Cr alloy,Ni-18.5Cr-16W alloy

Earthquake engineering research needs in light of lessons learned from the 2011 Tohoku earthquake

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Earthquake Engineering and Engineering Vibration 2014年第S1期13卷 141-149页

作者： Masayoshi Nakashima Oren Lavan Masahiro kurata Yunbiao Luo Disaster Prevention Research Institute (DPRI) Kyoto University Professor Disaster Prevention Research Institute (DPRI) Kyoto UniversityGokashoUjiKyotoJapan Faculty of Civil and Environmental Engineering Technion–Israel Institute of TechnologyHaifaIsrael Assistant Professor Post Doctoral Fellow

Earthquake engineering research and development have received much attention since the first half of the twentieth century. This valuable research presented a huge step forward in understanding earthquake hazard mitigation,which resulted in appreciable reduction of the effects of past earthquakes. Nevertheless,the 2011 Tohoku earthquake and the subsequent tsunami resulted in major damage. This paper presents the timeline of earthquake mitigation and recovery,as seen by the authors. Possible research directions where the authors think that many open questions still remain are identified. These are primarily based on the important lessons learned from the 2011 Tohoku earthquake.

关键词： research needs earthquake engineering quick recovery 2011 Tohoku earthquake

Antithrombin reduces reperfusion-induced hepatic metastasis of colon cancer cells

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World Journal of Gastroenterology 2006年第1期12卷 60-65页

作者： Masanao kurata Kenji Okajima Toru Kawamoto Mitsuhiro Uchiba Nobuhiro Ohkohchi Department of Surgery Functional and Regulatory Medical SciencesGraduate School of Comprehensive Human SciencesUniversity ofTsukubaTsukubaJapan Department of Biodefense Graduate School of Medical SciencesNagoya City UniversityNagoyaJapan Department of Diagnostic Medicine Graduate School of Medical SciencesKumamoto UniversityKumamotoJapan

AIM： To examine whether antithrombin （AT） could prevent hepatic ischemia/reperfusion （I/R）-induced hepatic metastasis by inhibiting tumor necrosis factor （TNF）-α-induced expression of E-selectin in rats. METHODS： Hepatic I/R was induced in rats and mice by clamping the left branches of the portal vein and the hepatic artery. Cancer cells were injected intrasplenically. The number of metastatic nodules was counted on day 7 after I/R. TNF-α and E-selectin mRNA in hepatic tissue, serum fibrinogen degradation products and hepatic tissue levels of 6-keto-PGF1α, a stable metabolite of PGI2, were measured. RESULTS： AT inhibited increases in hepatic metastasis of tumor cells and hepatic tissue mRNA levels of TNF-α and E-selectin in animals subjected to hepatic I/R. Argatroban, a thrombin inhibitor, did not suppress any of these changes. Both AT and argatroban inhibited I/R-induced coagulation abnormalities. I/R-induced increases of hepatic tissue levels of 6-keto-PGF1α were significantly enhanced by AT. Pretreatment with indomethacin completely reversed the effects of AT. Administration of OP-2507, a stable PGI2 analog, showed effects similar to those of AT in this model. Hepatic metastasis in congenital AT-deficient mice subjected to hepatic I/R was significantly increased compared to that observed in wild-type mice. Administration of AT significantly reduced the number of hepatic metastases in congenital AT-deficient mice. CONCLUSION： AT might reduce I/R-induced hepatic metastasis of colon cancer cells by inhibiting TNF-α-induced expression of E-selectin through an increase in the endothelial production of PGI2. These findings also raise the possibility that AT might prevent hepatic metastasis of tumor cells if administered during the resection of liver tumors.

关键词： Antithrombin,reperfusion Metastasis factor-aE-selectin Hepatic ischemia/Prostacyclin Tumor necrosis factor-α

Splenectomy in living donor liver transplantation and risk factors of portal vein thrombosis

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Hepatobiliary & Pancreatic Diseases International 2019年第4期18卷 337-342页

作者： Nobuhiko kurata Yasuhiro Ogura Satoshi Ogiso Yasuharu Onishi Hideya Kamei Yasuhiro Kodera Department of Transplantation and Endocrine Surgery(Surgery II) Nagoya University Graduate School of MedicineNagoyaJapan Department of Transplantation Surgery Nagoya University Hospital65 Tsurumai-choShowa-kuNagoya 466-8560Japan Department of Gastroenterological Surgery(Surgery II) Nagoya University Graduate School of MedicineNagoyaJapan

Background:Graft inflow modulation(GIM)during adult-to-adult living donor liver transplantation(LDLT)is a common strategy to avoid small-for-size syndrome,and some transplant surgeons attempt small size graft strategy with frequent GIM procedures,which are mostly performed by splenectomy,in ***,splenectomy can cause serious complications such as portal vein thrombosis and overwhelming postsplenectomy ***:Forty-eight adult-to-adult LDLT recipients were enrolled in this study and retrospectively *** applied the graft selection criteria,which routinely fulfill graft-to-recipient weight ratio≥0.8%,and consider GIM as a backup strategy for high portal venous pressure(PVP).Results:In our current strategy of LDLT,splenectomy was performed mostly due to hepatitis C and splenic arterial aneurysms,but splenectomy for GIM was intended to only one patient(2.1%).The final PVP values≤20 mmHg were achieved in all recipients,and no significant difference was observed in patient survival or postoperative clinical course based on whether splenectomy was performed or ***,6 of 18 patients with splenectomy(33.3%)developed postsplenectomy portal vein thrombosis(PVT),while none of the 30 patients without splenectomy developed PVT after *** was identified as a risk factor of PVT in this study(P<0.001).Our study revealed that a lower final PVP could be risk factor of postsplenectomy ***:Using sufficient size grafts was one of the direct solutions to control PVP,and allowed GIM to be reserved as a backup *** should be avoided as much as possible during LDLT because splenectomy was found to be a definite risk factor of *** splenectomy cases with a lower final PVP,a close follow-up is required for early detection and treatment of PVT.

关键词： Living donor liver transplantation Splenectomy Portal venous pressure Graft-to-recipient weight ratio Portal vein thrombosis

Pyrochemical treatment of spent nitride fuels for MA transmutation

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Science China Chemistry 2014年第11期57卷 1427-1431页

作者： HAYASHI Hirokazu SATO Takumi SHIBATA Hiroki kurata Masaki IWAI Takashi ARAI Yasuo Nuclear Science and Engineering Center Sector of Nuclear Science Research Japan Atomic Energy Agency2-4 Shirakata-shiraneTokai-muraNaka-gun Ibaraki-ken 319-1195 Japan

Nitride fuels have several advantages including high thermal conductivity and high metal density(like metallic fuels) and high melting point and isotropic crystal structure(like oxide fuels). Since the late 1990 s, the partitioning and transmutation of minor actinides(MA) has been studied to decrease the long-term radio-toxicity of high-level waste and to mitigate the burden of final disposal. Japan Atomic Energy Agency(JAEA) has proposed a dedicated transmutation cycle using an accelerator-driven system(ADS) with nitride fuels containing MA. The nitride fuel cycle we have developed includes a pyrochemical process. Our focus is on the electrolysis of nitride fuels and their refabrication from the recovered actinides; other processes are similar to the technology for metal fuel treatment and have been studied elsewhere. Here, we summarize our activity on the development of the pyrochemical treatment of spent nitride fuels.

关键词： nitride fuels minor actinides pyrochemical process

H_(∞)control in the frequency domain for a semi-active floor isolation system

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Frontiers of Structural and Civil Engineering 2013年第3期7卷 264-275页

作者： Yundong SHI Tracy C BECKER Masahiro kurata Masayoshi NAKASHIMA Graduate School of Engineering Kyoto UniversityKyoto 611-0011Japan Disaster Prevention Research Institute Kyoto UniversityKyoto 611-0011Japan

A floor isolation system installed in a single floor or room in a fixed base structure is designed to protect *** this configuration,the input motions to the floor isolation from the ground motions are filtered by the structure,leaving the majority of the frequency content of the input motion lower than the predominant frequency of the *** floor isolation system should minimize the acceleration to protect equipment;however,displacement must also be limited to save floor space,especially with long period ***-active control with an H_(∞)control was adopted for the floor isolation system and a new input shaping filter was developed to account for the input motion characteristics and enhance the effectiveness of the H_(∞)control.A series of shake table tests for a semi-active floor isolation system using rolling pendulum isolators and a magnetic-rheological damper were performed to validate the H_(∞)*** control using an oil damper was also tested for *** test results show that the H_(∞)control effectively reduced acceleration for short period motions with frequencies close to the predominant frequency of the structure,as well as effectively reduced displacement for long period motions with frequencies close to the natural frequency of the floor isolation *** H_(∞)control algorithm proved to be more advantageous than passive control because of its capacity to adjust control strategies according to the different motion frequency characteristics.

关键词： semi-active floor isolation H1 control MR damper shaping filter shaking table test

Recurrent renal cell carcinoma leading to a misdiagnosis of polycystic liver disease: A case report

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World Journal of Gastroenterology 2019年第18期25卷 2264-2270页

作者： Chen Liang Kazuhiro Takahashi Masanao kurata Shingo Sakashita Tatsuya Oda Nobuhiro Ohkohchi Department of Surgery University of Tsukuba Department of Diagnostic Pathology University of Tsukuba

BACKGROUND Polycystic liver disease(PCLD) with a large cystic volume deteriorates the quality of life of patients through substantial effects on the adjacent organs,recurrent cyst infections, cyst rupture, and hemorrhage. Surgical or radiological intervention is usually needed to alleviate these symptoms. We report a rare case of the cystic metastasis of renal cell carcinoma(RCC), which was misdiagnosed as PCLD, as a result of the clinical and radiological similarity between these *** SUMMARY A 74-year-old female who had undergone nephrectomy for papillary-type RCC(PRCC) was suffering from abdominal pain and the recurrent intracystic hemorrhage of multiple cysts in the liver. Imaging studies and aspiration cytology of the cysts showed no evidence of malignancy. With a diagnosis of autosomal dominant polycystic liver disease, the patient received hepatectomy for the purpose of mass reduction and infectious cyst removal. Surgery was performed without complications, and the patient was discharged on postoperative day 14. Postoperatively, the pathology revealed a diagnosis of recurrent PRCC with cystic *** This case demonstrates the importance of excluding the cystic metastasis of a cancer when liver cysts are observed.

关键词： Polycystic liver disease Polycystic kidney disease Cystic metastasis Renal cell carcinoma Case report