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World Journal of Diabetes 2023年第3期14卷 188-197页

作者： hiroshi nomoto Department of Rheumatology Endocrinology and NephrologyFaculty of Medicine and Graduate School of MedicineHokkaido UniversitySapporo 060-8638HokkaidoJapan

The maintenance of appropriate glycemic control is important for the prevention of diabetic complications in people with type 2 diabetes(T2D). Numerous oral antidiabetic drugs are now clinically available, but in particular, the introduction of injection regimens using insulin and/or glucagon-like peptide-1 receptor agonist(GLP-1RA)s represents promising step-up options for oral antidiabetic drug treatment. The recently licensed fixed-ratio combination(FRC) products,which comprise basal insulin and a GLP-1RA, have potent anti-hyperglycemic effects and reduce the undesirable side-effects of each component, such as body weight gain, hypoglycemia, and gastrointestinal symptoms. Two FRCs-insulin degludec/Liraglutide and insulin glargine/Lixisenatide-are now clinically available and, to date, several phase Ⅱ/Ⅲ trials have been conducted in particular groups of subjects with T2D. However, their utility in real-world clinical settings is of interest for most clinicians. Recently reported real-world clinical trials of these two FRCs in various situations have demonstrated their efficacy regarding glycemic control and the quality of life of people with T2D. Their long-term safety and efficacy require confirmation, but a treatment strategy that includes an FRC may be compatible with the concept of “well-balanced” therapy in certain groups of patients with T2D who have inadequate glycemic control.

关键词： Clinical trial Diabetes mellitus,type 2 Glucagon-like peptide-1 receptor Glycemic control Insulin,long-acting Quality of life

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东日本铁路公司开发的市郊列车

国外铁道车辆 2006年第6期43卷 10-15页

作者： hiroshi nomoto 周贤全

介绍了东日本铁路公司4个系列市郊列车的发展过程,并从车体、转向架、主电路、控制装置、制动装置等几方面进行了阐述。

关键词：市郊列车结构开发日本

Study on Distribution of Four Pseudomonas Species in Living Environment Using Multiplex PCR

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Open Journal of Stomatology 2024年第2期14卷 77-86页

作者： Sachiyo Hayashi Koji Umezawa Osamu Tsuzukibashi Akira Fukatsu Mana Fuchigami Chiaki Komine hiroshi Yamamoto Mio Hagiwara-Hamano Yukiko Iizuka Satoshi Uchibori Masanobu Wakami hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Takato nomoto Department of Special Needs Dentistry Nihon University School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine for Dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Surgery Nihon University School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.

关键词： Genus Pseudomonas Multiplex PCR Living Environment

稳定表达NGF、BDNF和NT3星形胶质细胞系的建立及其鉴定

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吉林大学学报（医学版） 2005年第2期31卷 227-230页

作者：马丽娜遇红梅赵文静洛涛陈森野元裕古川昭荣吉林大学再生医学研究所生化教研室吉林长春130021 名古屋大学歧阜药科大学

目的 :探讨星形胶质细胞直接作为转基因靶细胞进行目的基因的表达。方法 :克隆大鼠 NGF、BDNF和 NT3基因 ,构建真核表达载体 ;3种重组载体分别转染星形胶质细胞 ,G4 18筛选后获得阳性克隆细胞进行扩大培养 ;取基因修饰星形胶质细胞培养... 详细信息

目的 :探讨星形胶质细胞直接作为转基因靶细胞进行目的基因的表达。方法 :克隆大鼠 NGF、BDNF和 NT3基因 ,构建真核表达载体 ;3种重组载体分别转染星形胶质细胞 ,G4 18筛选后获得阳性克隆细胞进行扩大培养 ;取基因修饰星形胶质细胞培养液上清培养 PC12细胞或 Trk B- PC12细胞 ;用 Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。结果 :经 G4 18筛选后 ,转染 NGF、BDNF和 NT3基因修饰星形胶质细胞呈 G4 18抗性 ;转染 NGF和 NT3基因条件培养液上清培养的 PC12细胞 ,数量增多 ,突起明显变长 ,转染 BDNF基因条件培养液上清培养的 Trk B- PC12细胞 ,同样数量增多 ,突起明显变长。大部分 G4 18抗性的基因靶细胞出现 NGF、BDNF和 NT3免疫染色阳性。Western blotting杂交显示有相应分子量大小的蛋白条带出现。结论 :星形胶质细胞直接作为基因靶细胞能有效地表达和分泌有生物学活性的 NGF、 BDNF和 NT3。

关键词：星形细胞转化基因表达

Small sphincterotomy combined with papillary dilation with large balloon permits retrieval of large stones without mechanical lithotripsy

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World Journal of Gastroenterology 2007年第15期13卷 2179-2182页

作者： Atsushi Minami Shinji Hirose Tomohiro nomoto Shoichiro Hayakawa Internal Medicine Kawasaki Kyodo Hospital 2-1-5 Sakuramoto Kawasaki City 210-0833Japan

AIM： TO introduce a new method： small endoscopic sphincterotomy （ES） combined with endoscopic papillary large balloon dilation （SES ＋ EPLBD） to treat patients with large biliary stones.METHODS： Retrieval of large biliary stones was performed in 88 patients. Mean stone size was 14 ± 3 mm and mean number of stones was 2.5 ± 3.5. Firstly, ES with a small incision was performed. Next, endoscopic papillary dilation was performed with a large balloon to slowly match the size of the bile duct. Stones were then retrieved from the biliary duct with a balloon and a basket.RESULTS： Stone retrieval was successful in all cases except one cystic duct stone case without the need to crush large stones. Mean procedure time was 30 ± 5 min. Dilating the papillary orifice with a large balloon made it possible to remove large stones smoothly without crushing them. After dilation with the large balloon, there were some instances of oozing, but no perforations. One instance of post-procedural pancreatitis （1%） occurred. CONCLUSION： SES ＋ EPLBD was effective for the retrieval of large biliary stones without the use of mechanical lithotripsy.

关键词： Endoscopic sphincterotomy Endoscopic papillary dilation Bile duct stone

Obstructive jaundice caused by secondary pancreatic tumor from malignant solitary fibrous tumor of pleura:A case report

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World Journal of Gastroenterology 2006年第30期12卷 4922-4926页

作者： Norie Yamada Chiaki Okuse Masahito nomoto Mayu Orita Yoshiki Katakura Toshiya Ishii Takuo Shinmyo Hiroaki Osada Ichiro Maeda hiroshi Yotsuyanagi Michihiro Suzuki Fumio Itoh Division of Gastroenterology and Hepatology Department of Internal Medicine St. Marianna University School of Medicine 2-16-1 Sugao Miyamae-ku Kawasaki 216-8511 Japan Department of Chest Surgery St. Marianna University School of Medicine 2-16-1 Sugao Miyamae-ku Kawasaki 216-8511 Japan Department of Diagnostic Pathology St. Marianna University School of Medicine 2-16-1 Sugao Miyamae-ku Kawasaki 216-8511 Japan Division of Infectious Diseases Department of Internal Medicine University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-0033 Japan

A 77-year-old man on systemic chemotherapy against postoperative bilateral multiple lung metastases of malignant solitary fibrous tumor of the pleura suffered from pruritus and jaundice. Blood examination showed elevated levels of hepatobiliary enzymes. Abdominal computed tomography showed a tumor with peripheral enhancement in the pancreatic head, accompanied with the dilatation of intra- and extra-hepatic bile ducts. He was diagnosed as having obstructive jaundice caused by a pancreatic head tumor. The pancreatic head tumor was presumably diagnosed as the metastasis of malignant solitary fibrous tumor of the pleura, because the findings on the pancreatic head tumor on abdominal CT were similar to those on the primary lung lesion of malignant solitary fibrous tumor of the pleura. The pancreatic tumor grew rapidly after the implantation of metallic stent in the inferior part of the common bile duct. The patient died of lymphangitis carcinomatosa of the lungs. Autopsy revealed a tumor that spread from the pancreatic head to the hepatic hilum. Microscopically, spindle-shaped cells exhibiting nuclear atypicality or division together with collagen deposition were observed. Immunohistochemically the pancreatic head tumor cells were negative for staining of α-smooth muscle actin （α-SMA） or CD117, but positive for vimentin, CD34 and CD99. These findings are consistent with those on malignant solitary fibrous tumor of the pleura. We report the first case of obstructive jaundice caused by a secondary pancreatic tumor from malignant solitary fibrous tumor of the pleura.

关键词： Malignant solitary fibrous tumor of the pleura Secondary pancreatic tumor Obstructive jaundice

Evaluation of magnifying colonoscopy in the diagnosis of serrated polyps

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World Journal of Gastroenterology 2012年第32期18卷 4308-4316页

作者： Shinya Ishigooka Masahito nomoto Nobuyuki Obinata Yoshichika Oishi Yoshinori Sato Satoko Nakatsu Midori Suzuki Yoshiko Ikeda Tadateru Maehata Tomoaki Kimura Yoshiyuki Watanabe Takashi Nakajima Hiro-o Yamano hiroshi Yasuda Fumio Itoh Department of Internal Medicine Division of Gastroenterology and HepatologySt.Marianna University School of MedicineKawasaki 216-8511Japan Center of Gastroenterology St.Marianna Toyoko HospitalKawasaki 211-0063Japan Department of Gastroenterology Akita Red Cross HospitalAkita 010-1495Japan

AIM:To elucidate the colonoscopic features of serrated lesions of the colorectum using magnifying ***:Broad division of serrated lesions of the colorectum into hyperplastic polyps(HPs),traditional serrated adenomas(TSAs),and sessile serrated adenomas/polyps(SSA/Ps) has been proposed on the basis of recent molecular biological ***,few reports have examined the colonoscopic features of these divisions,including magnified colonoscopic *** study examined 118 lesions excised in our hospital as suspected serrated lesions after magnified observation between January 2008 and September *** characteristics(sex,age),conventional colonoscopic findings(location,size,morphology,color,mucin) and magnified colonoscopic findings(pit pattern diagnosis) were interpreted by five colonoscopists with experience in over 1000 colonoscopies,and were compared with histopathological *** pit patterns were categorized according to Kudo's classification,but a more detailed investigation was also performed using the subclassification [type Ⅱ-Open(type Ⅱ-O),type Ⅱ-Long(type Ⅱ-L),or type Ⅳ-Serrated(type Ⅳ-S)] proposed by Kimura T and Yamano ***:Lesions comprised 23 HPs(23/118:19.5%),39 TSAs(39/118:33.1%:with cancer in one case),50 SSA/Ps(50/118:42.4%:complicated with cancer in three cases),and six others(6/118:5.1%).We excluded six others,including three regular adenomas,one hamartoma,one inflammatory polyp,and one juvenile polyp for further *** colonoscopy showed that SSA/Ps were characterized as larger in diameter than TSAs and HPs(SSA/P vs HP,13.62 ± 8.62 mm vs 7.74 ± 3.24 mm,P < 0.001;SSA/Ps vs TSA,13.62 ± 8.62 mm vs 9.89 ± 5.73 mm,P < 0.01);common in the right side of the colon [HPs,30.4%(7/23):TSAs,20.5%(8/39):SSA/P,84.0%(42/50),P < 0.001];flat-elevated lesion [HPs,30.4%(7/23):TSAs,5.1%(2/39):SSA/Ps,90.0%(45/50),P < 0.001];normal-colored or pale imucosa [HPs,34.8%(8/23):TSAs,10.3%(4/39):SSA/Ps,80%(40/50),P < 0.00

关键词： Serrated adenoma Sessile serrated adenoma/polyp Hyperplastic polyps Traditional serrated adenomas Conventional colonoscopy Magnifying colonoscopy Pit patterns Serrated lesions

Dynamic Multi-Wavelength Assignment with Link Aggregation on SLAMNet Test System

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光学学报 2003年第S1期23卷 695-696页

作者： Hiroyuki Yokoyama Hajime Nakamura Shinichi nomoto KDDI R&D Laboratories Inc. 2-1-15 Ohara Kamifukuoka-shi Saitama 356-8502 JapanKDDI R&D Laboratories Inc. 2-1-15 Ohara Kamifukuoka-shi Saitama 356-8502 JapanKDDI R&D Laboratories Inc. 2-1-15 Ohara Kamifukuoka-shi Saitama 356-8502 Japan

This paper presents the implementation and performance of the best-effort multi-wavelength assignment with link aggregation on the SLAMNet (Statistical Lambda Multiplexing Network) test system.

关键词： link of than Test Dynamic Multi-Wavelength Assignment with Link Aggregation on SLAMNet Test System with

Risk factors of gastric cancer specific for tumor location and histology in Cali,Colombia

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World Journal of Gastroenterology 2006年第36期12卷 5772-5779页

作者： Francia Campos Gabriel Carrasquilla Chihaya Koriyama Mauricio Serra Edwin Carrascal Tetsuhiko Itoh Mitsuharu nomoto Suminori Akiba Depart-ment of Epidemiology and Preventive Medicine Kagoshima Uni-versity Graduate School of Medical and Dental Sciences 8-35-1 Sakuragaoka Kagoshima 890-8544 Japan Department of Microbiology Faculty of Health Sciences Universidad del Valle San Fernando Cali Co-lombia Health Division FES Foundation Cali Colom-bia Department of Pathology Faculty of Health Sciences Universidad del Valle San Fernando Cali Colombia Kaisei-en Geriatric Health Services Facility 551 Akasegawa Akune Kagoshima Japan Department of Human Pathology Kagoshi-ma University Graduate School of Medical and Dental Sciences 8-35-1 Sakuragaoka Kagoshima 890-8544 Japan

AIM- To examine histology- and tumor-location specific risk factors of gastric cancer （GC）.METHODS： This was subjects were 216 GC the period 2000-2002 non-cancer patients hospital. We obtained habits, and others by a a case-control study. The study patients newly diagnosed during and 431 controls selected from matching in age, gender, and information on lifestyles, dietary ***： The subjects who were not eldest among his/her siblings were at a slightly elevated GC risk （OR 1.3; 95% CI 0.8-2.0）. Salting meals before tasting was related to an increased GC risk （OR 3.5; 95% CI 1.6- 7.3）. Frequent consumptions of fruits （OR 0.3; 95% CI 0.1-1.0） and vegetables （OR 0.3; 95% CI 0.1-1.0） were related to decreased GC risks. On the other hand, frying foods （OR 1.9; 95% CI 1.0-3.6） and cooking with coal （OR 1.8; 95% CI 1.3-2.6） were related to increased GC risks. Neither Lauren＇s histological classification （intestinal and diffuse types） nor tumor location significantly affected those associations except birth order. The subjects who were not eldest among his/her siblings had an increased risk of GCs in the distal and middle thirds, and their ORs were 1.7 （95% CI 1.0-2.8） and 1.9 （95% CI 0.8-4.3）, respectively. The corresponding OR in the upper third stomach was 0.3 （95% CI 0.1-0.9）. The differences of those three ORs were statistically significant （P = 0.010）.CONCLUSION： The present study shows that birth order, salt intake, consumption of fruits and vegetables, the type of cooking, and cigarette smoking are related to GC risk. In histology and tumor-location specific analyses, non-eldest person among their siblings is related to an increased GC risk in the distal and middle thirds of the stomach, and is related to a decreased GC risk in the cardia.

关键词： Gastric cancer Risk factor Tumor location Histological type Colombia

Oncological problems in pancreatic cancer surgery

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World Journal of Gastroenterology 2006年第28期12卷 4466-4472页

作者： Akimasa Nakao Tsutomu Fujii Hiroyuki Sugimoto Naohito Kanazumi Shuji nomoto Yasuhiro Kodera Soichiro Inoue Shin Takeda Department of Surgery Ⅱ Nagoya University Graduate School of MedicineNagoyaJapan

Despite the development of more sophisticated diagnostic techniques, pancreatic carcinoma has not yet been detected in the early stage. Surgical resection provides the only chance for cure or long-term survival. The resection rate has increased due to recent advances in surgical techniques and the application of extensive surgery. However, the postoperative prognosis has been poor due to commonly occurring liver metastasis, local recurrence and peritoneal dissemination. Recent molecular-biological studies have clarified occult metastasis, micrometastasis and systemic disease in pancreatic cancer. Several oncological problems in pancreatic cancer surgery are discussed in the present review.

关键词： Pancreatic cancer Extended resection Molecular diagnosis Micrometastasis Adjuvant therapy