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To Investigate the Effect of Using Ethanol Containing Wipes in Collecting Blood for the Measurement of Alcohol Concentration

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Pharmacology & Pharmacy 2021年第10期12卷 208-218页

作者： Takami nakao Atsushi Nitta hiroshi Nishioka Munehiro Katagi Noriko Tsuda Yasuhide Kitazawa Critical Care Medical Center Kindai University Nara Hospital Ikoma City Japan Forensic Science Laboratory Osaka Prefectural Police Quarters Osaka Japan Critical Care Medical Center Kindai University Hospital Osaka-Sayama City Japan

This study was aimed to establish whether the skin preparation using ethanol-containing skin antiseptics causes ethanol contamination through blood collection. Venous blood was collected from 40 healthy volunteers according to the national guidelines for blood sampling, with four sequential procedures as follows: 1) collecting blood immediately (within 5 seconds) after cleaning the skin with an individually packaged type of ethanol-containing wipe, 2) collecting blood 1 minute after cleaning the skin with an individually packaged type of ethanol-containing wipe, 3) collecting immediately (within 5 seconds) after cleaning the skin with a traditional cleaning method (thoroughly ethanol-impregnated wipe, and 4) collecting 1 minute after cleaning the skin with a traditional cleaning method. Each sequential procedure was performed with and without the ethanol-containing wipe used for skin cleaning on the puncture site on their right and left arms at the time the needle was withdrawn, respectively. The collected specimens were subjected to the determination of ethanol by using headspace gas chromatography-mass spectrometry. In every 80 blood specimens obtained from 40 participants, ethanol was undetectable (<0.001 mg/mL). This study demonstrates that disinfection using ethanol-containing skin antiseptics is unlikely to cause ethanol contamination through blood collection regardless of skin preparation technique according to the guidelines for blood sampling. This may have implications in forensic science.

关键词： Blood Alcohol Content Skin Antiseptic Contamination of Ethanol

Water Saturation Dependence on CO<sub>2</sub>Sorption Potential of Sandstones

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Natural Resources 2012年第2期3卷 48-55页

作者： Takashi Fujii Kaori Endo Satomi Nakagawa Yoshiyuki Sato hiroshi Inomata Shinsuke nakao Toshiyuki Hashida Fracture and Reliability Research Institute Tohoku University Sendai Japan Institute for Geo-Resources and Environment National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba Japan Research Center of Supercritical Fluid Technology Tohoku University Sendai Japan Research Center of Supercritical Fluid Technology Tohoku University Sendai Japan.

For the assessment of the carbon dioxide (CO2) storage potential of water-filled reservoir rocks (i.e., saline aquifers), it should be first important step for a thorough understanding of the effect of water content on CO2/water/rock interactions during CO2 injection. The purpose of this study is to examine the CO2 sorption amount for Kimachi sandstone and Berea sandstone at different water content using the manometric method at temperature of 50?C and pressures of up to 20 MPa. Our results document that a significant quantity of CO2 was sorbed on the two types of sandstone on all water-saturated bases, which corresponded to the amount adsorbed on the air-dry basis. Also, all the wet samples had significantly higher sorption capacity than the theoretical values calculated from the solubility model based on dissolution of CO2 in pore water and the pore-filling model, which assumes that the pore volume unoccupied by water is filled with CO2. Furthermore, the observations indicated a certain degree of correlation between the sorbed amount and the water content, except at pressures below the critical point for Berea sandstone. This investigation points out that CO2 sorption is a possible mechanism in CO2 geological storage even under water-saturated conditions and that the mechanism of sorption on silica and silicate minerals plays an essential role in the reliable and accurate estimation of the CO2 storage capacity of water-saturated reservoirs.

关键词： CO2 Water Saturation Sorption Potential Sandstones

Expression of β-catenin in hepatocellular carcinoma

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World Journal of Gastroenterology 2005年第16期11卷 2398-2401页

作者： Liem Thanh Tien Masahiro Ito Mikiko nakao Daisuke Niino Meirmanov Serik Masahiro Nakashima Chun-Yang Wen hiroshi Yatsuhashi Hiromi Ishibashi Department of Pathology National Nagasaki Medical Center 2-1001-1 KubaraOmuraNagasaki 856-8562Japan Department of Molecular Pathology Atomic Bomb Disease Institute Nagasaki University Graduate School of Biomedical Science1-12-4 SakamotoNagasaki 852-8523Japan Tissue and Histopathology Section Division of Scientific Data Registry Atomic Bomb Disease Institute Nagasaki University Graduate School of Biomedical Science1-12-4 SakamotoNagasaki 852-8523Japan Department of Digestive Disease Nanjing Drum Tower Hospital Medical School of Nanjing UniversityNanjing 210008Jiangsu ProvinceChina Clinical Research Center National Nagasaki Medical Center 2-1001-1 KubaraOmuraNagasaki 856-8562Japan

AIM: The beta-catenin has been recognized as a critical member of the Wnt signaling pathway and plays an important role in the generation/differentiation of many tissues. Inappropriate activation of this pathway has been implicated in carcinogenesis. The mechanism underlying the development as well as its prognosis of hepatocellular carcinoma (HCC) has remained unclear. The purpose of this study is to analyze the expression of beta-catenin in HCC in relation to histological grades and viral hepatitis backgrounds. METHODS: Thirty-two sections were selected at random from autopsy and surgical cases of HCC. Immuohistologically, the location and positivity of beta-catenin expression in HCC was examined. RESULTS: Normal hepatocytes did not express beta-catenin. In 78% of HCC beta-catenin was expressed at the membrane of the cells, with or without cytoplasmic and/or nuclear expression. The tumor cells with well-and moderately-differentiated grades expressed frequently at the membrane and cytoplasm compared with poorly-differentiated type. Nuclear expression of beta-catenin was prone to occur in the tumor cells of poorly-differentiated grade. There were 15% of hepatitis C virus (HCV) backgrounds with nuclear expression. In contrast, there was 38% with nuclear expression in hepatitis B virus (HBV) backgrounds. In nonB-nonC hepatitis, no case expressed nuclear beta-catenin. CONCLUSION: The beta-catenin expression in HCC cells was heterogenous among types of hepatitis viral infection. Wnt signaling pathway might be deeply involved in less-differentiated HCC and HBV background. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

关键词： Hepatocellular carcinoma beta-Catenin Immunohistochemistry

Combination of genetic analysis and ancient literature survey reveals the diverge nee of traditional Brassica rapa varieties from Kyoto, Japan

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Horticulture Research 2021年第1期8卷 1877-1886页

作者： Yaichi Kawakatsu Tomoaki Sakamoto Hokuto Nakayama Kaori Kaminoyama Kaori Igarashi Masaki Yasugi hiroshi Kudoh Atsushi JNagano Kentaro Yano nakao Kubo Michitaka Notaguchi Seisuke Kimura Faculty of Life Sciences Kyoto Sangyo UniversityKamigamo-motoyamaKita-kuKyoto 603-8555Japan Bioscience and Biotechnology Center Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Center for Plant Sciences Kyoto Sangyo UniversityKamigamo-motoyamaKita-kuKyoto 6038555Japan Graduate School of Science The University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-0033Japan School of Agriculture Meiji University1-1-1Higashi-MitaTama-kuKawasakiKanagawa 214-8571Japan Faculty of Engineering Utsunomiya University7-1-2 YotoUtsunomiyaTochigi 321-8585Japan Center for Ecological Research Kyoto UniversityHirano 2-509-3OtsuShiga 520-2113Japan Faculty of Agriculture Ryukoku University1-5 YokotaniSeta Oe-choOtsuShiga 520-2194Japan Graduate School of Life and Environmental Sciences Kyoto Prefectural University1-5 Hangi-choShimogamoSakyo-kuKyoto 606-8522Japan Biotechnology Research Department Kyoto Prefectural AgricultureForestry and Fisheries Technology Center74 OjiKitainayazumaSeika-choSoraku-gunKyoto 6190244Japan Graduate School of Bioagricultural Sciences Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Institute of Transformative Bio-Molecules Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan

Since ancient times,humans have bred several plants that we rely on ***,little is known about the divergence of most of these *** the present study,we investigated the divergence of Mibuna(Brassica rapa *** ***),a traditional leafy vegetable in Kyoto(Japan),by combining genetic analysis and a survey of ancient *** is considered to have been bred 200 years ago from Mizuna,another traditional leafy vegetable in *** has simple spatulate leaves,whereas Mizuna has characteristic serrated *** quantitative trait loci(QTL)and gene expression analyses suggested that the downregulation of BrTCP15 expression contributed to the change in the leaf shape from serrated to simple ***,the SNP analysis indicated that the genomic region containing the BrTCP15 locus was transferred to Mibuna by ***,we conducted a survey of ancient literature to reveal the divergence of Mibuna and found that hybridization between Mizuna and a simple-leaved turnip might have occurred in the ***,the genomic analysis of multiple turnip cultivars showed that one of the cultivars,Murasakihime,has almost the same sequence in the BrTCP15 region as *** results suggest that the hybridization between Mizuna and turnip has resulted in the establishment of Mibuna.

关键词： cultivar survey analysis

Distinct Gene Expression Profile Distinguishes Increased Metabolic Activity in Spontaneously Hyperactive Rats While Sedentary from That Induced by Exercise

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Advances in Biological Chemistry 2018年第1期8卷 1-14页

作者： Manami Abe Yuki Matsuo Akiko Harada-Sukeno Takayuki Uchida Kanako Kitahata Chisato Tomida Katsuya Hirasaka Shigetada Teshima-Kondo Nagakatsu Harada Yutaka Nakaya hiroshi Sakaue Reiko nakao Takeshi Nikawa Departments of Nutritional Physiology Institute of Biomedical Sciences Tokushima University Graduate School Tokushima Japan Laboratory of Fishery Nutritional Science Graduate School of Fisheries and Environmental Sciences Nagasaki University Nagasaki Japan Department of Medical Nutrition Graduate School of Comprehensive Rehabilitation Osaka Prefecture University Osaka Japan Departments of Nutrition and Metabolism Institute of Biomedical Sciences Tokushima University Graduate School To-kushima Japan

The Spontaneously-Running Tokushima Shikoku (SPORTS) strain is an original line derived from Wistar rats, which spontaneously runs >6 km/day on wheels, and has better glucose tolerance and less fat than Wistar rats. However, the molecular mechanism that contributes to the increased metabolic activity in SPORTS rats is unknown. The present study aimed to characterize the gene expression profiles of skeletal muscles in SPORTS rats housed under sedentary (SED) conditions. We found that the expression levels of genes encoding mitochondrial respiratory chain enzymes such as ATP synthase 6 (mt-Atp6) and cytochrome c oxidase subunit 6c (Cox6c), were higher in the soleus (SOL) muscles of SED SPORTS than in SED Wistar rats. The ratio of type IIa myofibers was higher and glucose tolerance was better in SED SPORTS than in Wistar rats that were sedentary and trained daily on treadmills, respectively. We then investigated candidate genes that might contribute to the better glucose tolerance of SED SPORTS rats using DNA microarray analysis. Among 116 upregulated genes in the SOL muscles of SED SPORTS rats, only 19 were also increased in trained Wistar rats. We focused on v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (Erbb3), which was associated with glucose transport in myocytes, and found higher expression levels in the SOL muscles of SED SPORTS than in SED Wistar rats. The SOL muscles of SED SPORTS rats also contained more activity of β-hydroxyacylCoA dehydrogenase, a key enzyme of β-oxidation, indicating enhanced lipid oxidation. These findings suggest that increased metabolic activity in skeletal muscle (especially the SOL muscle) of SPORTS rats is congenital and that gene expression profiles of SPORTS rats and Trained Wistar rats are different.

关键词： Mitochondria Myofiber Type Microarray Metabolism

To Investigate the Effect of Using Ethanol Containing Wipes in Collecting Blood for the Measurement of Alcohol Concentration

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