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Secondary Metabolites Produced by an Endophytic Fungus Pestalotiopsis microspora

Natural Products and Bioprospecting 2019年第6期9卷 411-417页

作者： G.R.Nalin Rathnayake N.Savitri Kumar Lalith Jayasinghe hiroshi araya Yoshinori Fujimoto National Institute of Fundamental Studies Hantana RoadKandySri Lanka School of Agriculture Meiji UniversityKawasakiKanagawa 214‑8571Japan

An endophytic fungus Pestalotiopsis microspora isolated from the fruits of Manilkara zapota was cultured in potato dextrose broth *** separation of the EtOAc extract of the broth and mycelium led to the isolation of a new azaphilonoid named pitholide E(1),in addition to previously identified pitholide B(2),pitholide D(3),pestalotin(LL-P880α)(4),PC-2(5),LL-P880β(6),tyrosol(7)and 4-oxo-4H-pyran-3-acetic acid(8).An endophytic fungus *** from *** and the isolation of compounds 1-5,7 and 8 from *** are reported here for the first time.

关键词： Pestalotiopsis microspora Manilkara zapota Endophyte Pitholide E Pestalotin

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Production of small molecules by an endophytic fungus,Neofusicoccum parvum from the fruits of Elaeocarpus serratus

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Asian Journal of Traditional Medicines 2020年第2期15卷 47-54页

作者： Dilhara Dissanayake NSavitri Kumar NKBAdikaram Lalith Jayasinghe hiroshi araya Yoshinori Fujimoto National Institute of Fundamental Studies Hantana RoadKandySri Lanka School of Agriculture Meiji UniversityKawasakiKanagawa 214-8571Japan

Neofusicoccum parvum,an endophytic fungus isolated from Elaeocarpus serratus(Ceylon Olive;family Elaeocarpaceae),was grown for 3 weeks in potato dextrose *** separation of the ethyl acetate extracts from the culture filtrate and mycelium over silica gel,Sephadex LH-20 and preparative thin layer chromatography furnished(R)-7-hydroxymellein(1),(3R,4R)-4-hydroxymellein(2),(3R,4S)-4-hydroxymellein(3),(R)-5-hydroxymellein(4),(R)-mellein(5),(3R,4R)-4,7-dihydroxymellein(6),(6R,7S)-dia-asperlin(7),CJ-14445(8)and 13,14,15,16-tetranorlabd-7-ene-19,6β:12,17-diolide(9).The structures of known compounds 1–9 were determined by spectroscopic methods and comparison with reported *** is the first report of the isolation of an endophytic fungus from ***,and the isolation of compounds 1,4,6,8 and 9 from *** is important to note that compounds 1–7 are small molecules with an oxygen heterocyclic ring *** compounds can be used as starting materials in the synthesis of pharmaceutically important large molecules with oxygen heterocyles.

关键词： Neofusicoccum parvum Elaeocarpus serratus endophytic fungi secondary metabolites dihydroisocoumarines

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Secondary metabolites from an endophytic fungus Aspergillus fumigatus from Solanum insanum L.

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Asian Journal of Traditional Medicines 2021年第2期16卷 76-82页

作者： Vindya Kanthi Munasinghe N.Savitri Kumar Nimal Adikaram Lalith Jayasinghe hiroshi araya Yoshinori Fujimoto National Institute of Fundamental Studies Hantana RoadKandySri Lanka School of Agriculture Meiji UniversityKawasakiKanagawa214-8571Japan

An endophytic fungus Aspergillus fumigatus,isolated from the fruits of Solanum insanum L.,was cultured in potato dextrose broth *** separation of the EtOAc extract led to the isolation of six secondary metabolites,identified as pseurotin A(1),fumigaclavine C(2),monomethylsulochrin(3),trypacidin(4),fumiquinazoline C(5)and guignasulfide(6)by spectral *** 2 and 3 showed brine shrimp toxicity with IC50 values of 147 and 74.2μM,respectively,while compounds 4 and 5 showed slight inhibition on the growth of fungal pathogen Cladosporium *** is the first report of the isolation of guignasulfide(6)from ***,which has been previously isolated only from an endophytic fungus Guignardia sp.

关键词： Aspergillus fumigatus Endophytic fungi Solanum insanum Guignasulfide

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Audio-Visual Biofeedback for Respiratory Motion Management: Comparison of the Reproducibility of Breath-Holding between Visual and Audio Guidance

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Journal of Modern Physics 2018年第13期9卷 2286-2294页

作者： Naoki Sano Masahide Saito hiroshi Onishi Kengo Kuriyama Takafumi Komiyama Kan Marino Shinichi Aoki Masayuki araya Department of Radiology University of Yamanashi Yamanashi Japan

Purpose: To compare the reproducibility of breath-holding and comfort between two biofeedback guidance methods (audio and visual) in a respiratory monitoring system. Method and Materials: An audio-feedback respiratory monitoring device, which was modified from a visual-feedback self-respiration monitoring system (Abches, APEX Medical Inc., Tokyo, Japan) previously developed by the authors’ group, was constructed. Twenty patients (13 men, 7 women;mean age, 68.5 years;range, 54 - 85 years) with tumors in the thorax or abdominal region were enrolled in the present study. Computed tomography images were acquired from all patients three times using the two (i.e., audio and visual) respiration monitoring devices. To evaluate the reproducibility of breath-holding, the distance between an anatomical landmark and the tumor position was measured. Furthermore, patients were asked which guidance method they preferred (visual or audio) for comfortable breath-holding. Results: The two guidance methods improved the reproducibility of breath-holding compared with free-breathing, and no significant overall differences between two methods were observed (the mean displacements of the landmark-tumor distance were 2.60 ±1.38 mm and 2.35 ±1.63 mm, for visual and audio guidance, respectively). In five patients, the magnitude of position displacement in the series of three computed tomography images under audio guidance was twice as large as the other under visual guidance. Audio guidance was preferred to visual guidance by 65% (13 of 20) of the patients. However, the reproducibility of breath-holding did not always correspond with patient preference. Conclusion: There were some individual differences in the reproducibility of the visual and audio guidance methods. More appropriate, individualized guidance methods for each patient would improve the reproducibility of breath-holding in respiratory motion management.

关键词： Respiratory Motion Management

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