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Science China(Life Sciences) 2008年第2期51卷 133-139页

作者： Yutaka BANNO hiroshi fujii Institute of Genetic Resource Faculty of AgricultureKyushu University

The chromosomal locations of two single-copy genes, Ser-1 and CI-13, in silkworm (Bombyx mori) were detected at the molecular cytogenetics level by fluorescence in situ hybridization in the study. The results showed that Ser-1 is located near the distal end of the 11th linkage group, relatively at the 12.5±1.4 position in pachytene; and that CI-13 has been mapped near the distal end of the 2nd linkage group, relatively at the 8.2±1.2 position in pachytene. Furthermore, their location model map-FISH map on silkworm chromosome was drawn. The FISH technique and its application to silkworm are also discussed in this paper.

关键词： Bombyx mori sericin gene 1(Ser-1) chymotrypsin inhibitor 13 gene (CI-13) fluorescence in situ hybridization chromosome mapping

Cyclophosphamide-associated enteritis presenting with severe protein-losing enteropathy in granulomatosis with polyangiitis:A case report

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World Journal of Gastroenterology 2021年第20期27卷 2657-2663页

作者： Hiroko Sato Tsuyoshi Shirai hiroshi fujii Tomonori Ishii Hideo Harigae Department of Hematology and Rheumatology Tohoku University Graduate School of MedicineSendai 9808574Japan

BACKGROUND Although cyclophosphamide(CPA)is the key drug for the treatment of autoimmune diseases including vasculitides,it has some well-known adverse effects,such as myelosuppression,hemorrhagic cystitis,infertility,and ***,CPA-associated severe enteritis is a rare adverse effect,and only one case with a lethal clinical course has been ***,the appropriate management of patients with CPA-associated severe enteritis is *** SUMMARY We present the case of a 61-year-old woman diagnosed with granulomatosis with polyangiitis based on the presence of symptoms in ear,lung,and,kidney with positive myeloperoxidase-antineutrophil cytoplasmic *** received pulsed methylprednisolone followed by prednisolone 55 mg/d and intravenous CPA at a dose of 500 mg/*** days after the second course of intravenous CPA,she developed nausea,vomiting,and diarrhea,and was admitted to the *** testing revealed hypoalbuminemia,suggesting proteinlosing *** tomography revealed wall thickening of the stomach,small intestine,and colon with contrast enhancement on the lumen *** and immunosuppressive therapy were not effective,and the patient’s enteritis did not improve for>4 *** her condition became seriously exhausted,corticosteroids were tapered and supportive therapies including intravenous hyperalimentation,replenishment of albumin and gamma globulin,plasma exchange,and infection control were *** supportive therapies improved her condition,and her enteritis gradually *** was finally discharged 7 mo *** Immediate discontinuation of CPA and intensive supportive therapy are crucial for the survival of patients with CPA-associated severe enteritis.

关键词： Antineutrophil cytoplasmic antibody Cyclophosphamide Enteritis Granulomatosis with polyangiitis Plasma exchange Vasculitis Case report

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家蚕肾脏形卵(ki)的荧光差异显示研究

农业生物技术学报 2002年第3期10卷 262-266页

作者：李斌,夏庆友,hiroshi fujii,Yutaka Banno,鲁成西南农业大学农业部蚕桑学重点开放实验室西南农业大学农业部蚕桑学重点开放实验室 Institute of Genetic Resources Faculty of Agriculture Kyushu University Institute of Genetic Resources Faculty of Agriculture Kyushu University 西南农业大学农业部蚕桑学重点开放实验室重庆400716 重庆400716 6-10-1 Hakozaki Higashi-ku Fukuoka 812-8581 Japan 6-10-1 Hakozaki Higashi-ku Fukuoka 812-8581 Japan 重庆400716

为了探明家蚕(Bombyxmori )肾脏形卵的胚子不能正常发育的原因,使用FDD(flourescentdifferentdisplay)法对ki(kidney)基因同质型与异质型的母蛾所产未受精卵及受精24h卵的mRNA进行了差异显示研究。通过137组引物组合进行FDD差异显示,... 详细信息

为了探明家蚕(Bombyxmori )肾脏形卵的胚子不能正常发育的原因,使用FDD(flourescentdifferentdisplay)法对ki(kidney)基因同质型与异质型的母蛾所产未受精卵及受精24h卵的mRNA进行了差异显示研究。通过137组引物组合进行FDD差异显示,结果检出8254条扩增带。其中未受精卵与受精后24h卵之间的差异片段为206条,占总片段数的2.5%,而ki同质型与ki异质型的差异片段为141条,占总片段数的1.7%,其余的95.8%的扩增片段为4方共有,并且约60%的差异带是比较清晰的。回收了较明显的差异片段计199条,占总扩增带数的2.4%,并对其中部分差异片段进行序列分析表明,其中5个克隆序列分别为:smallheatshockproteins、proproteinconvertase、cancerantigenlikeprotein、fusionRNA-bindingprotein及receptorlikeproteinkinase同源序列,其余5个克隆序列未发现同源性,可能是新基因。

关键词：家蚕肾脏形卵荧光差异显示

Study on fibroin heavy chain of the silkworm Bombyx mori by fluorescence in situ hybridization (FISH)

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Science China(Life Sciences) 2002年第6期45卷 663-668页

作者：宋方洲张平波易发平洪锡钧鲁成 Yutaka Banno hiroshi fujii Katsumi Koga College of Basic Medical Sciences Chongqing University of Medical Sciences Chongqing 400016 China Key Sericultural Laboratory of Agriculture Ministry Southwest Agricultural University Chongqing 400716 China Key Sericultural Laboratory of Agriculture Ministry Southwest Agricultural University Chongqing 400716 China School of Life Science Southwest China Normal University Chongqing 400715 China

The sericulture industry plays a very important role in our national economy. Silkworm (Bombyx mori) is always regarded as a model animal and biological reactor. There have been detailed studies on the structure, expression and control and molecular evolution of silk genes. However, few, if any, reports are available on the localization of structural genes in silkworm by molecular cytogenetics. The present experiment has tentatively localized the Fib-H gene at the distal end of the 25th linkage group, namely at the 25-0.0 position, and verified that Fib-H has only one locus, thus providing a temporary solution to the problem about its localization.

关键词： silkworm Bombyx mori, fibroin gene, fluorescence in situ hybridization, gene localization.

家蚕丝胶基因1(Ser-1)和胰凝乳蛋白酶抑制因子13基因(CI-13)的FISH定位

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中国科学（C辑） 2008年第2期38卷 124-130页

作者：宋方洲常平安张平波易发平鲁成马永平 Banno Yutaka fujii hiroshi 重庆医科大学生物化学与分子生物学教研室重庆400016 西南大学蚕学与生物技术学院重庆400716 重庆邮电大学生物信息学院重庆400065 临床检验诊断学教育部重点实验室重庆400016 Institute of Genetic Resource Faculty of Agriculture Kyushu University Fukuoka 812-8581 Japan

应用荧光原位杂交技术对家蚕单拷贝的丝胶基因1(Ser-1)及胰凝乳蛋白酶抑制因子13基因(CI-13)进行了分子细胞遗传学的染色体定位.结果表明:Ser-1位于第11连锁群染色体的近端部位置,在粗线期染色体上的相对位置为12.5±1.4;CI-13位于第2... 详细信息

应用荧光原位杂交技术对家蚕单拷贝的丝胶基因1(Ser-1)及胰凝乳蛋白酶抑制因子13基因(CI-13)进行了分子细胞遗传学的染色体定位.结果表明:Ser-1位于第11连锁群染色体的近端部位置,在粗线期染色体上的相对位置为12.5±1.4;CI-13位于第2连锁群染色体的近端部,在粗线期染色体上的相对位置为8.2±1.2,进而绘制了上述基因在家蚕染色体上的位置模式图—FISH图,并对家蚕染色体的荧光原位技术及其应用进行了探讨.

关键词：家蚕(Bombyx mori) 丝胶基因1 胰凝乳蛋白酶抑制因子13基因荧光原位杂交染色体定位

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家蚕35K蛋白酶基因的组织表达

农业生物技术学报 2002年第3期10卷 259-261页

作者：姜永煌,徐孟奎,Takahiro Kusakabe,hiroshi fujii,陈玉银,吴玉澄浙江大学动物科学学院浙江大学动物科学学院 Faculty of Agriculture Kyushu University Faculty of Agriculture Kyushu University 浙江大学动物科学学院浙江大学动物科学学院杭州310029 杭州310029 Fukuoka 8128581 Japan Fukuoka 8128581 Japan 杭州310029 杭州310029

用RT-PCR方法分析了家蚕(Bombyxmori )35K蛋白酶基因在家蚕幼虫期的组织及发育经时性表达。结果表明:35K蛋白酶基因只在幼虫的中肠中部有特异性的表达,在中肠后部有微弱的表达,而在中肠前部及脂肪体、丝腺、精巢、卵巢和血球细胞等组... 详细信息

用RT-PCR方法分析了家蚕(Bombyxmori )35K蛋白酶基因在家蚕幼虫期的组织及发育经时性表达。结果表明:35K蛋白酶基因只在幼虫的中肠中部有特异性的表达,在中肠后部有微弱的表达,而在中肠前部及脂肪体、丝腺、精巢、卵巢和血球细胞等组织中不表达;在幼虫期,该基因在中肠组织中的表达量与幼虫摄食活动相关联,认为受该基因控制的35K蛋白酶与营养物质的消化吸收有关,在家蚕的生长发育过程中起重要作用。

关键词： 35K蛋白酶基因组织表达家蚕

Polyarteritis nodosa clinically mimicking nonocclusive mesenteric ischemia

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World Journal of Gastroenterology 2013年第23期19卷 3693-3698页

作者： Tsuyoshi Shirai hiroshi fujii Shinichiro Saito Tomonori Ishii Hideyuki Yamaya Shigehito Miyagi Satoshi Sekiguchi Naoki Kawagishi Masato Nose Hideo Harigae Department of Hematology and Rheumatology Tohoku University Graduate School of MedicineSendai 980-8574 Japan Division of Advanced Surgical Science and Technology Tohoku University Graduate School of MedicineSendai 980-8574 Japan Department of Histopathology Tohoku University Graduate School of MedicineSendai 980-8574 Japan

Here, we present the case of a 74-year-old Japanese man with segmental intestinal necrosis, which developed after treatment with pulsed methylprednisolone for mononeuritis multiplex. The patient was weakly positive for myeloperoxidase (MPO)-anti-neutrophil cytoplasmic antibody (ANCA). Computed tomography and surgical findings were compatible with nonocclusive mesenteric ischemia (NOMI). He underwent small intestinal resection by emergency surgery and an intestinal fistula was made. Pathologically, necrotizing vasculitis with fibrinoid necrosis was present in medium to small-sized arteries, which was equivalent to Arkin's classification Ⅱ-Ⅳ. Most of the arteries had fibrous intimal thickening, which was considered to obstruct the arteries and thus cause segmental intestinal necrosis. A diagnosis of polyarteritis nodosa (PAN) was made, and intravenous cyclophosphamide pulse therapy was added to the therapeutic regimen. This patient was successfully treated with these multidisciplinary therapies and his stoma was finally closed. This is a very rare and indicative case of PAN weakly positive for MPO-ANCA and clinically mimicking NOMI, which occurred even after treatment with pulsed methylprednisolone.

关键词： Anti-neutrophil cytoplasmic antibody Intestinal necrosis Myeloperoxidase Nonocclusive mesenteric ischemia Polyarteritis nodosa

低分子量热激蛋白Bmhsp19.9基因的表达

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农业生物技术学报 2005年第2期13卷 195-201页

作者：李斌夏庆友藤井博伴野丰鲁成西南农业大学农业部蚕桑学重点开放实验室西南农业大学农业部蚕桑学重点开放实验室重庆400716 日本九州大学基因资源开发研究中心九州812-8581

利用RT-PCR技术,对家蚕(Bombyxmori)低分子量热激蛋白Bmhsp19.9基因进行了定量表达分析。Bmhsp19.9在各组织中均有一定量的表达,但不同组织间的表达差异达10多倍,表达量丰富的组织是生长发育旺盛的组织,如精巢和卵巢,然后是丝腺、蛹等... 详细信息

利用RT-PCR技术,对家蚕(Bombyxmori)低分子量热激蛋白Bmhsp19.9基因进行了定量表达分析。Bmhsp19.9在各组织中均有一定量的表达,但不同组织间的表达差异达10多倍,表达量丰富的组织是生长发育旺盛的组织,如精巢和卵巢,然后是丝腺、蛹等组织。其应激表达模式可分为3类,以其在精巢、后部丝腺和脂肪体中的强应激表达模式为主,经热刺激处理后其表达量有显著增加。经刺激诱导2h后其表达量达到最高,随后逐渐降低,在热刺激诱导12h后是未刺激的2￣3倍,而此时Bmhsp19.9在脂肪体中的应激表达是其对照的10多倍。根据Bmhsp19.9在不同组织中的基本表达量、应激表达量和应激表达的持续时间各不相同,显示其与细胞分裂与分化等生命活动有一定联系。并认为其在后部丝腺、脂肪体、精巢、卵巢与血液中作用方式不一样,推测其作为分子伴侣在组织间的作用对象与作用效应也应有所差异。

关键词：家蚕低分子量热激蛋白 Bmhsp19.9基因应激表达

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野桑蚕、蓖麻蚕及家蚕基因组的RFLP分析

昆虫知识 2005年第6期42卷 635-638页

作者：常平安宋方洲易发平鲁成刘运强伴野丰藤井博重庆邮电学院生物信息学院重庆400065 重庆医科大学分子生物学教研室重庆400016 西南农业大学蚕丝学院农业部蚕桑学重点开放实验室重庆400716 日本九州大学农学部

以家蚕Bombyx mori丝素重链基因、丝胶基因1和胰凝乳蛋白酶抑制因子13基因为探针,对野桑蚕***、蓖麻蚕Philosamia cynthia ricini和家蚕***基因组DNA进行限制性片段长度多态性分析。结果发现,在野桑蚕、蓖麻蚕基因组中存在着家蚕丝素重... 详细信息

以家蚕Bombyx mori丝素重链基因、丝胶基因1和胰凝乳蛋白酶抑制因子13基因为探针,对野桑蚕***、蓖麻蚕Philosamia cynthia ricini和家蚕***基因组DNA进行限制性片段长度多态性分析。结果发现,在野桑蚕、蓖麻蚕基因组中存在着家蚕丝素重链基因、丝胶基因1的同源序列,而在中日野桑蚕以及蓖麻蚕品种间存在着限制性酶切位点差异;丝胶基因1在中国野桑蚕基因组的EcoRⅠ酶切图谱较日本野桑蚕与家蚕更为一致,表明家蚕与中国野桑蚕亲缘关系更近。此外,在野桑蚕基因组中发现了家蚕胰凝乳蛋白酶抑制因子13基因的同源序列,并且在家蚕品种间以及中日野桑蚕之间也存在着多态性。这些结果表明不同绢丝昆虫在适应生存环境的进化过程中,基因组发生了结构改变。

关键词：野桑蚕蓖麻蚕家蚕限制性片段长度多态性进化

Control of Secondary Flow in a Low Solidity Circular Cascade Diffuser

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Journal of Thermal Science 2012年第4期21卷 384-390页

作者： Daisaku Sakaguchi Takuji fujii Hironobu Ueki Masahiro Ishida hiroshi Hayami Nagasaki University 1-14 Bunkyo-machi Kyushu University (retired)

According to the previous experimental works on the low solidity circular cascade diffuser (LSD), a pressure recovery of a centrifugal blower was improved by the LSD significantly in a wide range of flow rate, and the pres-sure recovery was improved further by the LSD with a tandem cascade in comparison with the LSD with a sin-gle-row cascade. In the present study, the flow behavior in the LSD with the tandem cascade has been analyzed numerically by using the commercial CFD code of ANSYS-CFX12. It was shown clearly that the higher pressure recovery was achieved by applying the LSD with the tandem cascade, and the high pressure recovery is based on the high pressure rise in the vaneless space upstream of the LSD and the high blade loading of the front blade of the LSD. The high pressure recovery in the LSD could be achieved by controlling the flow separation on the suc-tion surface of the front blade and also on that of the rear blade due to formation of the favorable secondary flow and due to increase in mass flow passing through the slit section between the front and rear blades.

关键词： LSD (Low solidity circular cascade diffuser) Centrifugal blower Range enhancement Tandem cascade