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Ultrastructural changes in porcine liver sinusoidal endothelial cells of machine perfused liver donated after cardiac death

引用

World Journal of Gastroenterology 2022年第19期28卷 2100-2111页

作者： Hiroki Bochimoto Yo Ishihara Nur Khatijah Mohd Zin Hiroyoshi Iwata Daisuke Kondoh hiromichi obara Naoto Matsuno Department of Cell Physiology The Jikei University School of MedicineMinato-ku 105-8461TokyoJapan Department of Transplantation Technology and Therapeutic Development Asahikawa Medical UniversityAsahikawa 078-8510HokkaidoJapan Department of Surgery Asahikawa Medical UniversityAsahikawa 078-8510HokkaidoJapan Laboratory of Veterinary Anatomy Obihiro University of Agriculture and Veterinary MedicineObihiro 080-8555HokkaidoJapan Department of Mechanical Engineering Tokyo Metropolitan UniversityHachioji 192-0397TokyoJapan

BACKGROUND The machine perfusion(MP)preservation including hypothermic MP(HMP)and midthermic MP(MMP)has been considered as a promising strategy to preserve the functions of liver donated after cardiac *** importance of understanding liver sinusoidal endothelial cells(LSEC)damage in regulating liver injury during MP has been ***,the ultrastructural changes in the LSEC and sinusoids around them after MP are *** To investigate the ultrastructural changes in the LSEC and sinusoids around them after *** Porcine liver grafts undergo a warm ischemia time of 60 minutes perfused for 4 h with modified University of Wisconsin gluconate *** A grafts were preserved with HMP at 8℃ constantly for 4 *** B grafts were preserved with a rewarming solution at 22℃ by MMP for 4 *** the ultrastructural changes in the LSEC and sinusoids in Group A and B were comparatively analyzed by using osmium-maceration scanning electron microscopy with complementary transmission electron microscopy *** An analysis of the LSEC after warm ischemia revealed that mitochondria with condensed-shaped cristae,abnormal vesicles,reduction of ribosomes and the endoplasmic reticulum(ER)surround the mitochondria *** MP subsequent after warm ischemia alleviate the abnormal vesicles and reduction of ribosomes in LSEC,which indicated the reduction of the ER ***,MMP could restore the tubular mitochondrial cristae,while after HMP the condensed and narrow mitochondrial cristae *** addition,the volume of the sinusoidal space in the liver grafts after MMP were restored,which indicated a lower risk of pressure injury than *** MMP alleviates the ER damage of LSEC by warm ischemia,additionally restore the metabolism of LSEC via the normalization of mitochondria and prevent the share stress damage of liver grafts.

关键词： Liver Sinusoidal endothelial cells Warm ischemia Machine perfusion preservation Transplantation Ultrastructure

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