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Identification of cellular genes showing differential expression associated with hepatitis B virus infection

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World Journal of Hepatology 2012年第4期4卷 139-148页

作者： Yasuo Fukuhara Takeshi Suda Makoto Kobayashi Yasushi tamura Masato Igarashi Nobuo Waguri hirokazu Kawai Yutaka Aoyagi Division of Gastroenterology and Hepatology Graduate School of Medical and Dental Sciences Niigata University 1-757 Asahimachi-dori Chuo-ku Niigata Niigata 951-8122 Japan

AIM: To investigate the impact of hepatitis B virus (HBV) infection on cellular gene expression, by conducting both in vitro and in vivo studies. METHODS: Knockdown of HBV was targeted by stable expression of short hairpin RNA (shRNA) in huH-1 cells. Cellular gene expression was compared using a human 30K cDNA microarray in the cells and quantified by real-time reverse transcription-polymerase chain reaction (RT-PCR) (qRT-PCR) in the cells, hepatocellular carcinoma (HCC) and surrounding non-cancerous liver tissues (SL). RESULTS: The expressions of HBsAg and HBx protein were markedly suppressed in the cells and in HBx transgenic mouse liver, respectively, after introduction of shRNA. Of the 30K genes studied, 135 and 103 genes were identified as being down- and up-regulated, respectively, by at least twofold in the knockdown cells. Functional annotation revealed that 85 and 62 genes were classified into four up-regulated and five down-regulated functional categories, respectively. When gene expression levels were compared between HCC and SL, eight candidate genes that were confirmed to be up- or down-regulated in the knockdown cells by both microarray and qRT-PCR analyses were not expressed as expected from HBV reduction in HCC, but had similar expression patterns in HBV- and hepatitis C virus-associated cases. In contrast, among the eight genes, only APM2 was constantly repressed in HBV non-associated tissues irrespective of HCC or SL. CONCLUSION: The signature of cellular gene expression should provide new information regarding the pathophysiological mechanisms of persistent hepatitis and hepatocarcinogenesis that are associated with HBV infection.

关键词： Hepatitis B virus Differential gene expression Hepatocellular carcinoma Gene expression signature Adipose most abundant 2

Shear wave velocity is a useful marker for managing nonalcoholic steatohepatitis

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World Journal of Gastroenterology 2010年第23期16卷 2918-2925页

作者： Akihiko Osaki Tomoyuki Kubota Takeshi Suda Masato Igarashi Keisuke Nagasaki Atsunori Tsuchiya Masahiko Yano Yasushi tamura Masaaki Takamura hirokazu Kawai Satoshi Yamagiwa Toru Kikuchi Minoru Nomoto Yutaka Aoyagi Department of Gastroenterology and Hepatology Graduate School of Medical and Dental SciencesNiigata University Department of Homeostatic Regulation and Development Graduate School of Medical and Dental SciencesNiigata University Department of Laboratory Medicine Niigata University Medical and Dental Hospital

AIM:To investigate whether a noninvasive measurement of tissue strain has a potential usefulness for management of nonalcoholic steatohepatitis(NASH).METHODS:In total 26 patients,23 NASHs and 3 normal controls were enrolled in this *** was staged based on Brunt *** a region of interest(ROI),a shear wave was evoked by implementing an acoustic radiation force impulse(ARFI),and the propagation velocity was quantif ***:Shear wave velocity(SWV) could be reproducibly quantified at all ROIs in all subjects except for 4 NASH cases,in which a reliable SWV value was not calculated at several *** average SWV of 1.34 ± 0.26 m/s in fibrous stage 0-1 was significantly slower than 2.20 ± 0.74 m/s and 2.90 ± 1.01 m/s in stages 3 and 4,respectively,but was not significantly different from 1.79 ± 0.78 m/s in stage *** a cutoff value was set at 1.47 m/s,receiver operating characteristic analysis showed significance to dissociate stages 3 and 4 from stage 0-1(P=0.0092) with sensitivity,specificity and area under curve of 100%,75% and 94.2%,*** addition,the correlation between SWV and hyaluronic acid was significant(P<0.0001),while a tendency toward negative correlation was observed with serum albumin(P=0.053).CONCLUSION:The clinical implementation of ARFI provides noninvasive repeated evaluations of liver stiffness at an arbitrary position,which has the potential to shed new light on NASH management.

关键词： Nonalcoholic steatohepatitis Ultrasound Liver stiffness measurement Shear wave velocity Acoustic radiation force impulse

Clinical Impact of Bacteremia Due to <i>Staphylococcus saprophyticus</i>

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Advances in Infectious Diseases 2021年第1期11卷 6-12页

作者： Daisuke tamura Hiroaki Yamane Hikaru Tabakodani hirokazu Yamagishi Erika Nakazato Yumiko Kimura Masayoshi Shinjoh Takanori Yamagata Department of Pediatrics Jichi Medical University Tochigi Japan Department of Clinical laboratory Jichi Medical University Hospital Tochigi Japan Department of Pediatrics Keio University School of Medicine Tokyo Japan

Objective: This was a retrospective study to evaluate the clinical impact of bacteremia due to Staphylococcus saprophyticus and identify which factors influence it. Methods: We reviewed all patients with bacteremia due to S. saprophyticus over the last 12 years. This study was performed at Jichi Medical University Hospital in Japan, a key hospital in the northern Kanto area including Tochigi, Gunma, Ibagagi, and northern Saitama prefectures. We retrospectively reviewed the blood culture results and medical records of all patients with a history of visits or hospitalizations between April 2008 and September 2020. Results: During the study period, 4 blood culture specimens were considered to have S. saprophyticus bacteremia. Two of these were from subjects > 60 years old who had severe infection. A third case, 27 years old, was thought to have a catheter-related bloodstream infection;however, the only symptom was fever, which was not serious. The fourth case, a previously unreported pediatric patient, had non-severe fever. The mean time for a diagnosis of bacteremia by blood culture testing was 42 hours, whereas contamination appeared in cultures after another 50 hours. Conclusion: The pathogenicity of S. saprophyticus might be lower in the blood than in the urine due to its physiological function and activity. In older adults with underlying diseases, the severity of bacteremia was more pronounced, whereas in a young adult and a child, the disease was relatively mild. Age and underlying disease might be useful factors to consider when diagnosing bacteremia due to S. saprophyticus.

关键词： Gram-Positive Staphylococcus Coagulase-Negative Staphylococcus Staphylococcus saprophyticus Bacteremia

Multicentric occurrence of hepatocellular carcinoma with nonalcoholic steatohepatitis

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World Journal of Hepatology 2011年第1期3卷 15-23页

作者： hirokazu Kawai Minoru Nomoto Takeshi Suda Kenya Kamimura Atsunori Tsuchiya Yasushi tamura Masahiko Yano Masaaki Takamura Masato Igarashi Toshifumi Wakai Satoshi Yamagiwa Yasunobu Matsuda Shogo Ohkoshi Isao Kurosaki Yoshio Shirai Masahiko Okada Yutaka Aoyagi Department of Clinical Laboratory Niigata University Medical and Dental Hospital Division of Gastroenterology and Hepatology Niigata University Graduate School of Medical and Dental Sciences Division of Digestive and General Surgery Niigata University Graduate School of Medical and Dental Sciences Department of Medical Technology Niigata University Graduate School of Health Sciences Division of Preventive Medicine Niigata University Graduate School of Medical and Dental Sciences

AIM:To reveal the manner of hepatocellular carcinoma (HCC) development in patients with nonalcoholic steatohepatitis(NASH) focusing on multicentric occurrence (MO) of ***:We compared clinicopathological characteristics between patients with and without MO of HCC arising from NASH *** clinical features were implicated with reference to the literature ***:MO of HCC was identified with histological proof in 4 out of 12 patients with NASH-related HCC(2 males and 2 females).One patient had synchronous MO;an advanced HCC,two well-differentiated HCCs and a dysplastic nodule,followed by the development of metachronous MO of *** other three patients had multiple advanced HCCs accompanied by a well-differentiated HCC or a dysplastic *** these three patients,one had synchronous MO,one had metachronous MO and the other had both synchronous and metachronous *** were no obvious differences between the patients with or without MO in terms of liver function tests,tumor markers and anatomical extent of *** the other hand,all four patients with MO of HCC were older than 70 years old and had the comorbidities of obesity,type 2 diabetes mellitus(T2DM),hypertension and *** these conditions were not limited to MO of HCC,all the conditions were met in only one of eight patients without MO of ***,concurrence of these conditions may be a predisposing situation to synchronous MO of *** particular,old age,T2DM and cirrhosis were suggested to be prerequisite for MO because these factors were depicted in common among two other cases with MO of HCC under NASH in the ***:The putative predisposing factors and necessary preconditions for synchronous MO of HCC in NASH were suggested in this *** investigations are required to clarify the accurate prevalence and predictors of MO to establish better strategies for treatment and prevention leading to the prognostic improvement in NASH.

关键词： Nonalcoholic steatohepatitis Hepatocellular carcinoma Multicentric occurrence

Active treatments are a rational approach for hepatocellular carcinoma in elderly patients

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World Journal of Gastroenterology 2013年第24期19卷 3831-3840页

作者： Takeshi Suda Aiko Nagashima Shyunsaku Takahash Tsutomu Kanefuji Kenya Kamimura Yasushi tamura Ma-saaki Takamura Masato Igarashi hirokazu Kawai Satoshi Yamagiwa Minoru Nomoto Yutaka Aoyagi Division of Gastroenterology and Hepatology Graduate School of Medical and Dental Sciences Niigata University

AIM: To determine whether an active intervention is beneficial for the survival of elderly patients with hepa-tocellular carcinoma (HCC). METHODS: The survival of 740 patients who received various treatments for HCC between 1983 and 2011 was compared among different age groups using Cox regression analysis. Therapeutic options were principal-ly selected according to the clinical practice guidelines for HCC from the Japanese Society of Hepatology. The treatment most likely to achieve regional control capa-bility was chosen, as far as possible, in the following order: resection, radiofrequency ablation, percutaneous ethanol injection, transcatheter arterial chemoembo-lization, transarterial oily chemoembolization, hepatic arterial infusion chemotherapy, systemic chemotherapy including molecular targeting, or best supportive *** treatment was used alone, or in combination, with a clinical goal of striking the best balance be-tween functional hepatic reserve and the volume of the targeted area, irrespective of their age. The percent survival to life expectancy was calculated based on a Japanese national population survey. RESULTS: The median ages of the subjects during each 5-year period from 1986 were 61, 64, 67, 68 and 71 years and increased significantly with time (P<0.0001). The Child-Pugh score was comparable among younger (59 years of age or younger), middle-aged (60-79 years of age), and older (80 years of age or older) groups (P=0.34), whereas the tumor-node-metastasis stage tended to be more advanced in the younger group (P=0.060). Advanced disease was significantly more frequent in the younger group compared with the middle-aged group (P=0.010), whereas there was no difference between the middle-aged and elderly groups (P=0.75). The median sur-vival times were 2593, 2011, 1643, 1278 and 1195 d for 49 years of age or younger, 50-59 years of age, 60-69 years of age, 70-79 years of age, or 80 years of age or older age groups, respectively, whereas the me-di

关键词： Hepatocellular carcinoma Population ag-ing Survival Life expectancy Active intervention

Clinical Impact of Bacteremia Due to Staphylococcus saprophyticus

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