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Exploring the biogeography,morphology,and phylogeny of the condylostomatid ciliates(Alveolata,Ciliophora,Heterotrichea),with establishment of four new Condylostoma species and a revision including redescriptions of five species found in China

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Marine Life Science & Technology 2024年第3期6卷 365-404页

作者： Yong Chi Fan Wei Danxu Tang Changjun Mu Honggang Ma Zhe Wang Khaled A.SAl-Rasheid Hunter N.hines Xiangrui Chen School of Marine Sciences Ningbo UniversityNingbo 315211China Institute of Evolution&Marine Biodiversity and Key Laboratory of Evolution&Marine Biodiversity(Education Ministry)Ocean University of ChinaQingdao 266003 China3 Marine College Shandong UniversityWeihai 264209China Weishan Special Aquaculture Base Jining 277600China Zoology Department College of SciencesKing Saud UniversityRiyadh 11451Saudi Arabia Harbor Branch Oceanographic Institute Florida Atlantic UniversityFlorida 34946USA

Species of the ciliate class Heterotrichea Stein,1859 are a cosmopolitan group of unicellular eukaryotic microorganisms,many of which have been widely used as models in various fields of research such as regenerative biology,functional ecol-ogy,environmental toxicology,and symbiotic ***,species identification in the heterotrich family Condylos-tomatidae,especially the most species-rich and type genus Condylostoma Bory de Saint-Vincent,1824,remains challenging due to incomplete original descriptions,few reliable distinguishing characters,and overlapping features between different *** study presents an updated revision of Condylostoma and its related genus Condylostomides da Silva Neto,1994 based on descriptions of five species,including nine populations collected from China,using both morphological and molecular *** main findings are as follows:(1)43 nominal species and about 130 populations are reviewed,result-ing in the recognition of 30 valid species of Condylostoma and eight valid species of Condylostomides;(2)keys,synonyms,biogeographic distributions and amended/improved diagnoses of all valid species are provided;(3)based on the available data,four new Condylostoma species(*** ***.,*** ***.,*** ***.,and *** ***.),one new combination(Condylostomides minimus(Dragesco,1954)***.&***.),and two corrected names(Condylostoma ancestrale Villeneuve-Brachon,1940 *** Condylostomides nigrus(Dragesco,1960)***.)are suggested;(4)cryptic species are detected and proposed for the first time to form the Condylostoma curvum spe-cies complex;(5)three highly confusing Condylostoma species,***,***,and ***,are redefined for the first time based on modern taxonomic methods;(6)a‘flagship’species,Condylostomides coeruleus,is recorded for the first time from the continent of Asia,substantially expanding its biogeography;(7)ciliature adjacent to the distal end of the paroral mem

关键词： Condylostoma Condylostomides New species Phylogeny Revision Taxonomy

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Multi-gene-based investigation on the molecular phylogeny of the hypotrichous family Strongylidiidae(Protista,Ciliophora),with notes on the ontogeny of a new genus and new species

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Marine Life Science & Technology 2024年第3期6卷 442-461页

作者： Wenya Song Shijing Zhang Yuqing Li Honggang Ma Qiyu Li Xiaotian Luo Khaled A.S.Al-Rasheid Hunter N.hines Xiaoteng Lu Institute of Evolution&Marine Biodiversity Ocean University of ChinaQingdao 266003China Key Laboratory of Aquatic Biodiversity and Conservation of Chinese Academy of Sciences Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China Weishan Special Aquaculture Base Jining 277600China Zoology Department King Saud University11451 RiyadhSaudi Arabia Harbor Branch Oceanographic Institute Florida Atlantic UniversityFort PierceFlorida 34946USA Department of Biology Shenzhen MSU-BIT UniversityShenzhen 518172China

Ciliates in the subclass Hypotrichia have long been difficult to classify as they are one of the most polymorphic and highly differentiated groups,leading to their systematics remaining *** relationships within the hypotrich family Strongylidiidae have been ambiguous due to discordance between the morphological and genetic *** this study,a new strongylidiid genus Heterouroleptus is established,mainly based on the novel mode of origin of the ventral cirral rows:left ventral cirral row(LVR)originates from frontal-ventral-transverse cirral anlagen(FVTA)Ⅲ(anterior portion),IV(middle portion),and V(rear portion);right ventral cirral row comes from the entire FVTA VI.A new species,Hetero-uroleptus weishanensis ***.,***.,is investigated along with the morphometric and molecular data from a population of Strongylidium *** new sequences and nuclear gene markers(single-gene and multi-gene)are provided to analyze the phylogenetic relationships of strongylidiids,with the COI gene utilized to uncover further genetic information at species level and *** results reveal that:(1)Strongylidiidae is monophyletic and has a close relationship with Dorsomarginalia;(2)Heterouroleptus *** a clade that is sister to all the other strongylidiids;(3)Hemiamphisiella Foissner,1988 and Pseudouroleptus Hemberger,1985 should not be synonyms,and both genera should be subdivided due to their variable morphological characteristics;(4)LVR originating from three anlagen is a plesiomorphy of *** discovery of the origin of the LVR not only contributes to the establishment of the genus Heterouroleptus,but also helps to improve the diagnosis of the family Strongylidiidae.

关键词： Ciliates Cytochrome c oxidase subunit I gene Morphogenesis Phylogeny·Ribosomal RNA gene Taxonomy

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Skits in English New ed.

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1980年

作者： hines Mary Elizabeth.

来源：畅想之星图书光盘版（南... 评论

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Natural killer T cells and non-alcoholic fatty liver disease:Fat chews on the immune system

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World Journal of Gastroenterology 2008年第3期14卷 487-488页

作者： Michael Kremer Ian N hines Department of Surgery University of Heidelberg Heidelberg 69120 Germany Department of Medicine University of North Carolina at Chapel Hill CB 7525 United States

Natural killer T cells (NKT) are an important subset of T lymphocytes. They are unique in their ability to produce both T helper 1 and T helper 2 associated cytokines, thus being capable of steering the immune system into either inflammation or tolerance. Disruption of NKT cell numbers or function results in severe deficits in immune surveillance against pathogens and tumor cells. Growing experimental evidence suggests that hepatosteatosis may reduce resident hepatic as well as peripheral NKT cells. Those models of hepatosteatosis and the change in NKT cell numbers are associated with a disruption of cytokine homeostasis, resulting in a more pronounced release of proinflammatory cytokines which renders the steatotic liver highly susceptible to secondary insults. In this letter to the editor, we focus on recently published data in the World Journal of Gastroenterology by Xu and colleagues demonstrating reduced peripheral NKT cells in patients with non-alcoholic fatty liver disease, compare those findings with ours and others in different animal models of hepatosteatosis, and hypothesize about the potential underlying mechanism.

关键词： Obesity Hepatosteatosis Metabolic syndrome TNFalpha Interleukin 12

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New guidelines for the treatment of severe acute pancreatitis

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Hepatobiliary Surgery and Nutrition 2022年第6期11卷 913-916页

作者： Gabriel L.Oland O.Joe hines Department of Surgery David Geffen School of Medicine at UCLALos AngelesCAUSA

Severe forms of pancreatitis account for 15-20%of acute pancreatitis cases,with a mortality rate approaching 20%(1,2).Patients with this condition manifest components of multisystem organ failure,rapid decompensation,and life-threatening complications arising both early and late in the disease *** clinical decline can be difficult to predict,and treatments are typically supportive and reactive,rather than preventative(3).These factors pose complex challenges during treatment,which has evolved over the past decade and requires a team approach to management.

关键词： pancreatitis acute mortality

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Morphological and molecular examination of the ciliate family Lagynusidae(Protista,Ciliophora,Prostomatea)with descriptions of two new genera and two new species from China

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Marine Life Science & Technology 2023年第2期5卷 178-195页

作者： Limin Jiang Congcong Wang Saleh A.Al-Farraj Hunter N.hines Xiaozhong Hu College of Fisheries and Key Laboratory of MaricultureMinistry of EducationOcean University of ChinaQingdao 266003China Institute of Evolution and Marine Biodiversity Ocean University of ChinaQingdao 266003China Department of Zoology College of ScienceKing Saud UniversityRiyadh 11451Saudi Arabia Harbor Branch Oceanographic Institute Florida Atlantic UniversityFort PierceFL 34982USA

Ciliates in the class Prostomatea play an important role in the global microbial loop due to their signifcant abundances and broad feeding strategies at the foundation of food *** their importance in ecosystems,the taxonomy and systematics of this group of ciliates has long been poorly understood,with this being especially true for members of the family *** we examine four lagynusids collected from sandy beaches in China,using silver-staining and 18S rRNA gene sequencing *** investigations revealed two new genera and two new species and provided details for two little known forms:Penardella marina ***.,***.,Apolagynus cucumis(as reported by Penard.Études sur les infusoires d’eau *** and Cie,Genève,1922)***.,***.,Lagynus minutus ***.,and Lagynus elegans(Engelmann in Z Wiss Zool 11:347-393,1862)Quennerstedt(Acta Univ Lund 4:1-48,1867).Penardella *** be morphologically distinguished by having more than three dikinetidal perioral *** *** from the closely related genus Lagynus in the absence of a conspicuous neck-like *** ciliature of Apolagynus cucumis is revealed here for the frst time,which demonstrates the classifcation of this species within ***,Apolagynus binucleatus(Jiang et al.,2021)*** established according to the new *** results of our phylogenetic analyses based on the 18S rRNA gene support the establishment of two new genera and indicate that Lagynusidae is monophyletic,which further strengthens its valid taxonomic status.

关键词： Ciliate Morphology New genus New species Phylogeny 18S rRNA gene

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Adjuvant chemotherapy in curatively resected rectal cancer: How valid are the data?

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World Journal of Gastrointestinal Oncology 2020年第4期12卷 503-513页

作者： Giulia Manzini Fabius Hapke Ian N hines Doris Henne-Bruns Michael Kremer Department of General and Visceral Surgery University of UlmUlm 89081Germany Department of Nutrition Science College of Allied Health SciencesEast Carolina UniversityGreenvilleNC 27834United States Department of General and Visceral Surgery Hospital of AarauAarau 5000Switzerland

BACKGROUND According to the result of the Cochrane review published in 2012,postoperative adjuvant chemotherapy(CTx)is associated with a survival benefit for rectal cancer patients operated for cure in comparison to patients who underwent only the surgical *** To analyze the quality of the data supporting the advantage of adjuvant CTx after surgery for rectal *** the times of increasing health care costs,it is imperative to offer the patient an evidence-based therapy that justifies potential side effects as well as *** Overall survival was selected as endpoint of *** the 21 included papers which analyzed this endpoint,we identified those three publications which have the highest weights to influence the final *** validity of these papers was analyzed using the CONSORT checklist for randomized controlled *** performed a second meta-analysis excluding the three analyzed studies(n=18)in order to assess their impact on the overall result of the original ***,we performed a third meta-analysis excluding all studies(n=16)which showed a statistically improved overall *** The detailed analysis of the three most relevant RCTs according to the items of the CONSORT checklist showed several *** up to 47%of the items,inappropriate answers were ***,a lack of information regarding therandomization procedure as well as the absence of allocation concealment,blinded set-up,of intention-to-treat analysis and omission of sample size calculation were common problems of the analyzed *** exclusion of these three studies from the meta-analysis did not affect the general result of the meta-analysis,still confirming a survival advantage after adjuvant *** exclusion of single studies with a statistically significant outcome improvement,the meta-analysis of the remaining 16 studies again shows a statistically significant result due in part to a large remaining sample size.C

关键词： Rectal cancer Validity Meta-analysis CONSORT checklist Postoperative chemotherapy Overall survival

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Becoming-Music in 4 Minutes and 33 Seconds

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Journal of Literature and Art Studies 2016年第4期6卷 411-417页

作者： Dorothea hines Trent University Peterborough Canada

In accordance to Langerian aesthetic theory, Mark Campbell （1992） concludes that Cage＇s 4 33＂（1952） is by no means aesthetic music. I argue the antithesis： Cage＇s 433＂ satisfies Langerian aesthetic theory, and is indeed ＂aesthetic＂ music. Cage does something more： he satisfies Langerian aesthetic theory, yet he is not limited by it. He does not simply create music, nor does he offer listeners a musical space. He creates what Gilles Deleuze and Fe1ix Guattari （1987） call a line of becoming that passes between music making and a musical space. In 4 minutes and 33 seconds of silence, Cage presents a sense of emptiness and numbness felt simultaneously with fullness and explosion. In what appears to be stillness, the listener experiences the flux of movement; what appears to be devoid of depth, is filled with complexities. 433＂ embraces chance, uncertainty, and the unknown; it is an experimental process; it is becoming-music in 4 minutes and 33 seconds.

关键词： John Cage Langerian aesthetic theory Gilles Deleuze Fo1ix Guattari line of becoming music ontology

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290K下0.1～600MPa水的Raman光谱原位研究

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光谱学与光谱分析 2004年第8期24卷 963-965页

作者：孙樯郑海飞谢鸿森徐济安 hines E 北京大学地球与空间科学学院北京100871 中国科学院地球化学研究所贵州贵阳550002 Carnegie Institute of Washington Charles and Colvard

在 2 90K和 0 0 1～ 6 0 0MPa条件下 ,就压力对液态水对称伸缩振动的影响进行了原位Raman散射光谱研究。表明 :( 1)在 0 1～ 2 0 0MPa范围内 ,(ν1) max随着压力升高而减小 ;( 2 )在 2 0 0～ 4 0 0MPa范围内 ,压力升高导致 (ν1) max... 详细信息

在 2 90K和 0 0 1～ 6 0 0MPa条件下 ,就压力对液态水对称伸缩振动的影响进行了原位Raman散射光谱研究。表明 :( 1)在 0 1～ 2 0 0MPa范围内 ,(ν1) max随着压力升高而减小 ;( 2 )在 2 0 0～ 4 0 0MPa范围内 ,压力升高导致 (ν1) max增大 ,并在 4 0 0MPa时达到最大值 ;( 3) (ν1) max随着压力的进一步升高 ( >4 0 0MPa)而降低。这表明在 2 0 0和 4 0 0MPa时 ,液态水结构的变化是不连续的 ,这也与在该压力范围内rOO 的变化特征是一致的。可认为与冰Ⅰh→冰Ⅲ→冰Ⅴ的相变相类似。

关键词：液态水压力对称伸缩振动原位Raman散射光谱相变氢键

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蚁群优化最小二乘支持向量机在测量数据拟合中的应用

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核动力工程 2018年第6期39卷 156-160页

作者：蒋波涛 hines J.Wesley 赵福宇西安工程大学电子信息学院西安710048 田纳西大学核工系诺克斯维尔田纳西37996 西安交通大学核科学与技术学院西安710049

针对传统数据拟合方法存在依赖用户经验,需预先确定估计拟合函数等缺点,提出一种基于蚁群优化最小二乘支持向量回归机(ACO-LSSVR)的数据拟合方法。该方法采用蚁群优化(ACO)对最小二乘支持向量回归机(LSSVR)的参数进行优化,获取最优参数... 详细信息

针对传统数据拟合方法存在依赖用户经验,需预先确定估计拟合函数等缺点,提出一种基于蚁群优化最小二乘支持向量回归机(ACO-LSSVR)的数据拟合方法。该方法采用蚁群优化(ACO)对最小二乘支持向量回归机(LSSVR)的参数进行优化,获取最优参数,从而建立数据拟合模型。将该方法与传统回归拟合方法用于核工程的2个测量数据拟合实例中,得到堆芯功率曲线和熔融液滴在冷却剂中运动特性曲线,将2条曲线的拟合结果进行了比较。结果表明,ACO-LSSVR具有较高的拟合精度且无需对数据分段确定拟合函数。

关键词：数据拟合核工程蚁群优化(ACO) 最小二乘支持向量回归机(LSSVR)

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