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Chinese Journal of Chemical Physics 2008年第3期21卷 193-201页

作者： Xi Xing Beth Reed Mi Kyung Bahng Peng Wang hin koo woo Sun Jong Baek Chee Shing Lain Cheuk Yiu Ng Department of Chemistry University of California Davis Davis California 95616 USA

We show that by scanning the frequency of a single mode infrared （IR） optical parametric oscillator （IR- OPO） laser to excite the molecular species of interest and fixing the frequency of a vacuum ultraviolet （VUV） laser to photoionize the IR excited species, high-resolution IR spectra of polyatomic neutrals can be obtained with high sensitivity. The fact that this IR-VUV-photoion （IR-VUV-PI） method is based on VUV photoionization probe, and thus, allows the identification of the neutral IR absorber, makes it applicable for IR spectroscopy measurements of isotopemers, radicals, and clusters, which usually exist as impure samples. The highly resolved IR-VUV-PI measurements achieved using the single mode IR-OPO laser have made possible the selection of single rovibrational states of CH3X （X=Br and I）, C2H4, and C3H4 for VUV-pulsed field ionization-photoelectron （VUV-PFI-PE） measurements, resulting in rovibrationally resolved photoelectron spectra for these polyatomic molecules. These experiments show that the signal- to-noise ratios of the IR-VUV-PI and IR-VUV-PFI-PE spectra obtained by employing the high-resolution IR-OPO laser are significantly higher than those observed in previous IR-VUV-PI and IR-VUV-PFI-PE studies using a low-resolution IR-OPO laser. Further improvement in sensitivity of IR-VUV-PI and IR- VUV-PFI-PE measurements by using the collinear arrangement of IR-VUV lasers and molecular beam is discussed.

关键词： Vacuum ultraviolet Infrared Pulse-field photoelectron High resolution

Transparent high-performance piezoceramics through pressureless sintering

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Journal of Advanced Ceramics 2024年第5期13卷 561-567页

作者： Hye-Lim Yu woo-Seok Kang Ju-Hyeon Lee Temesgen Tadeyos Zate Young-Jin Lee Bo-Kun koo Dong-Jin Shin Min-Soo Kim Soon-Jong Jeong Young Ghyu Ahn wook Jo Department of Material Science and Engineering Ulsan National Institute of Science and TechnologyUlsan 44919Republic of Korea Energy Conversion Research Center Korea Electrotechnology Research InstituteChangwon 51543Republic of Korea MLCC Development Team Component Biz.UnitSamsung Electro-Mechanics Co.Ltd.Suwon 16674Republic of Korea

Piezoelectricity offers an electromechanical coupling that is widely utilized in transducer *** has been a consistent demand for transparent piezoelectric materials for optoelectrical ***,despite the inherent tradeoff between the transparency and the piezoelectricity,numerous strategies have been explored to develop the transparent piezoelectric ***,the most transparent piezoelectric materials developed to date is either a single crystal or materials that achieve transparency via hot-press sintering,limiting its industrial ***,we introduce a novel piezoelectric material that ensures transparency through co-doping and pressureless sintering of polycrystalline *** this study,we employed a compositional optimization approach to enhance the synergistic effect between the transparency and the piezoelectric properties of 0.71Pb(Mg_(1/3)Nb_(2/3))O_(3)–0.29PbTiO_(3)(PMN–0.29PT)*** utilizing the tape casting process for mass production and large-area manufacturing,our Pb_(0.913)La_(0.0145)Sm_(0.0145)(Mg_(1/3)Nb_(2/3))_(0.71)Ti_(0.29)O_(3)(TP2.9)ceramics exhibited over 60%transparency and large piezoelectric coefficient(d33)of 1104 pC/*** material holds considerable promise for a wide range of industrial applications in both the optical and electronic domains.

关键词： piezoelectric ceramics transparency pressureless sintering co-doping tape casting

Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

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Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-koo Yi Jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

Identification of novel small-molecule inhibitors of SARS-CoV-2 by chemical genetics

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Acta Pharmaceutica Sinica B 2024年第9期14卷 4028-4044页

作者： Chris Chun-Yiu Chan Qian Guo Jasper Fuk-woo Chan Kaiming Tang Jian-Piao Cai Kenn Ka-Heng Chik Yixin Huang Mei Dai Bo Qin Chon Phin Ong Allen Wing-Ho Chu Wan-Mui Chan Jonathan Daniel Ip Lei Wen Jessica Oi-Ling Tsang Tong-Yun Wang Yubin Xie Zhenzhi Qin Jianli Cao Zi-Wei Ye hin Chu Kelvin Kai-Wang To Xing-Yi Ge Tao Ni Dong-Yan Jin Sheng Cui Kwok-Yung Yuen Shuofeng Yuan State Key Laboratory of Emerging Infectious Diseases Carol Yu Centre for InfectionDepartment of MicrobiologySchool of Clinical MedicineLi Ka Shing Faculty of Medicinethe University of Hong KongPokfulamHong Kong SAR 999077China Department of Infectious Diseases and Microbiology the University of Hong Kong-Shenzhen HospitalShenzhen 518000China Centre for Virology Vaccinology and TherapeuticsHong Kong Science and Technology ParkHong Kong SAR 999077China Academician Workstation of Hainan Province Hainan Medical University-the University of Hong Kong Joint Laboratory of Tropical Infectious DiseasesHaikou 571100China School of Biomedical Sciences Li Ka Shing Faculty of Medicinethe University of Hong KongPokfulamHong Kong SAR 999077China NHC Key Laboratory of Systems Biology of Pathogens Institute of Pathogen Biology Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China College of Biology Hunan Provincial Key Laboratory of Medical VirologyHunan UniversityChangsha 410082China

There are only eight approved small molecule antiviral drugs for treating *** them,four are nucleotide analogues(remdesivir,JT001,molnupiravir,and azvudine),while the other four are protease inhibitors(nirmatrelvir,ensitrelvir,leritrelvir,and simnotrelvir-ritonavir).Antiviral resistance,unfavourable drug‒drug interaction,and toxicity have been reported in previous *** there is a dearth of new treatment options for *** this work,a three-tier cell-based screening was employed to identify novel compounds with anti-SARS-CoV-2 *** compound,designated 172,demonstrated broad-spectrum antiviral activity against multiple human pathogenic coronaviruses and different SARS-CoV-2 variants of *** studies validated by reverse genetics showed that compound 172 inhibits the 3-chymotrypsin-like protease(3CLpro)by binding to an allosteric site and reduces 3CLpro dimerization.A drug synergistic checkerboard assay demonstrated that compound 172 can achieve drug synergy with nirmatrelvir in *** vivo studies confirmed the antiviral activity of compound 172 in both Golden Syrian Hamsters and K18 humanized ACE2 ***,this study identified an alternative druggable site on the SARS-CoV-23CLpro,proposed a potential combination therapy with nirmatrelvir to reduce the risk of antiviral resistance and shed light on the development of allosteric protease inhibitors for treating a range of coronavirus diseases.

关键词： SARS-CoV-2 High throughput screening Broad-spectrum antiviral treatment 3CLpro inhibitor Allosteric-site inhibitor Animal models Chemical genetics Reverse genetics

Gene expression profiles in gastric mucosa of sleep deprivation rats

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World Journal of Gastroenterology 2000年第5期6卷 754-758页

作者： Jenny F.L.Chow Marcel W.L.koo Chi-hin Cho Department of Pharmacology Faculty of MedicineUniversity of Hong Kong5 Sassoon RoadPokfulamHong KongChina

INTRODUCTIONStress has been shown to induce gastric mucosallesions and lower the effectiveness of the mucosa asa *** rats,gastric ulcers can beproduced by cold-restraint stress and it isfrequently employed as a model ... 详细信息

关键词： gene expression mucosa gastric stress ulcer GI tract sleep deprivation cDNA ethanol

Effects of Ag Seed on Synthesis of FeCo Nano-Particles Prepared via the Polyol Method

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Journal of Materials Science & Technology 2010年第7期26卷 660-664页

作者： Uk-Rae Cho Geun-woo Kim Bon-Heun koo School of Nano and Advanced Materials Engineering Changwon National UniversityChangwonGyeongnam641-773Korea

Nano-particles have several interesting properties which are not shown in bulk materials because of their high ratio of surface area to volume. FeCo alloy nano-particles with soft magnetic properties are demanded for various applications such as optics, eletronics and magnetics. Through the polyol method, highly purified particles with mono-dispersibility in various sizes can be produced and used as high-tech functional materials. In this study, the magnetic characteristics of FeCo alloy with Ag seed added in the production process of mono-dispersed nano-particles through the polyol method were investigated.

关键词： Polyol method FeCo Ag Seed

Enhanced refolding of lysozyme with imidazolium-based room temperature ionic liquids: Effect of hydrophobicity and sulfur residue

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Science China Chemistry 2012年第8期55卷 1657-1662页

作者： BAE Sang-woo CHANG woo-Jin koo Yoon-Mo HA Sung Ho Department of Biological Engineering Inha University Incheon 402-751 Republic of Korea Department of Mechanical Engineering School of Freshwater Sciences University of Wisconsin-Milwaukee Milwaukee WI 53201-0413 USA Department of Chemical Engineering and Nano-Bio Technology Hannam University Daejeon 305-811 Republic of Korea

The expression of recombinant proteins in microorganism frequently leads to the formation of insoluble aggregates, inclusion bodies （IBs）. Thus, the additional in vitro protein refolding process is required to convert inactive IBs into water-soluble active proteins. This study investigated the effect of sulfur residue and hydrophobicity of imidazolium-based room temperature ionic liquids （RTILs） on the refolding of lysozyme as a model protein in the batch dilution method which is the most commonly used refolding method. When lysozyme was refolded in the refolding buffer containing [BF4]-based RTILs with a systematic variety of alkyl chain on cations varying from two to eight, less hydrophobic imidazolium cations having shorter alkyl chains were effective to facilitate lysozyme refolding. Compared to the conventional refolding buffer, 2 times higher lysozyme re- folding yield was obtained in l-ethyl-3-methylimidazolium tetrafluoroborate （[EMIM][BF4]） containing refolding buffer. The refolding yield of lysozyme was even more increased by 2.5 times when 1-butyl-3-methylimidazolium methylsulfate （[BMIM][MS]） containing sulfur residue on anion was used. The sulfur residue in [BMIM][MS] is supposed to improve the refolding yield of lysozyme which has 4 intrarnolecular disulfide bonds. For dilution-based refolding of lysozyme, the opti- mum concentrations of RTILs in refolding buffer were found to be 1.0 M [EMIM][BF4] and 0.5 M [PMIM][MS], respectively. The optimum temperate for dilution-based refolding of lysozyme with RTILs was 4 ℃.

关键词： protein refolding room temperature ionic liquid dilution refolding lysozyme hydrophobicity sulfur

Effects of Temperature on Photosynthetic Rates in Korean Fir (Abies koreana) between Healthy and Dieback Population

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Journal of Integrative Plant Biology 2008年第2期50卷 190-193页

作者： Su Young woo Jong-Hwan Lim Don koo Lee Department of Environmental Horticulture University of Seoul Seoul 130-743 Republic of Korea Korea Forest Research Institute Seoul 130-712Republic of Korea Department of Forest Sciences College of Agriculture and Life SciencesSeoul National UniversitySeoul 151-921Republic of Korea

The present study was carried out on natural Korean fir forests （Abies koreana） growing in Mount Halla in Jeju Island, Korea （33° 13-36＇ N and 126° 12-57＇ E）. Mount Halla is the highest mountain （1 950 m a.s.h） in South Korea. On the Korean fir forests near the top of Mount Halla in Korea, we established permanent plots between dieback and healthy population. Each permanent plot includes both dieback and relatively healthy Korean fir individuals. Three sites in this study showed similar altitude, topographic position, aspects, slope, diameter at breast height, average height and ages. Net photosynthetic rates （PN） on different temperature regimes were evaluated to explain the forest dieback phenomenon on Korean fir populations. Light response curves were determined on three different temperature regimes： 15 ℃, 20℃ and 25℃. The irradiance response curve showed higher values in lower air temperatures. Generally, irradiance response curves of healthy Korean fir populations were higher than the dieback population at all sites.

关键词： Abies koreana global warming forest decline photosynthetic rates temperature.

Mesenchymal stem cell therapy for cirrhosis: Present and future perspectives

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World Journal of Gastroenterology 2015年第36期21卷 10253-10261页

作者： Young woo Eom Gaeun Kim Soon koo Baik Cell Therapy and Tissue Engineering Center Yonsei University Wonju College of Medicine College of Nursing - Research Institute for Nursing Science Keimyung University Department of Internal Medicine Yonsei University Wonju College of MedicineWonju Severance Christian Hospital

Cirrhosis occurs as a result of various chronic liver injuries,which may be caused by viral infections,alcohol abuse and the administration of drugs and chemicals. Recently,bone marrow cells(BMCs),hematopoietic stem cells(HSCs) and mesenchymal stem cells(MSCs) have been used for developing treatments for cirrhosis. Clinical trials have investigated the therapeutic potential of BMCs,HSCs and MSCs for the treatment of cirrhosis based on their potential to differentiate into hepatocytes. Although the therapeutic mechanisms of BMC,HSC and MSC treatments are still not fully characterized,the evidence thus far has indicated that the potential therapeutic mechanisms of MSCs are clearer than those of BMCs or HSCs with respect to liver regenerative medicine. MSCs suppress inflammatory responses,reduce hepatocyte apoptosis,increase hepatocyte regeneration,reverse liver fibrosis and enhance liver functionality. This paper summarizes the clinical studies that have used BMCs,HSCs and MSCs in patients with liver failure or cirrhosis. We also present the potential therapeutic mechanisms of BMCs,HSCs and MSCs for the improvement of liver function.

关键词： Cirrhosis Cell therapy Bone marrow cells Mesenchym

Invasive and non-invasive diagnosis of cirrhosis and portal hypertension

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World Journal of Gastroenterology 2014年第15期20卷 4300-4315页

作者： Moon Young Kim woo Kyoung Jeong Soon koo Baik Department of Internal Medicine Yonsei University Wonju College of MedcineWonju Severance Christan HospitalWonju 220-701South Korea Department of Radiology and Center for Imaging Science Samsung Medical CenterSungkyunkwan University School of MedicineSeoul 440-746South Korea

With advances in the management and treatment of advanced liver disease,including the use of antiviral therapy,a simple,one stage description for advanced fibrotic liver disease has become *** refining the diagnosis of cirrhosis to reflect disease heterogeneity is essential,current diagnostic tests have not kept pace with the progression of this new *** biopsy and hepatic venous pressure gradient measurement are the gold standards for the estimation of hepatic fibrosis and portal hypertension(PHT),respectively,and they have diagnostic and prognostic ***,they are invasive and,as such,cannot be used repeatedly in clinical *** ideal noninvasive test should be safe,easy to perform,inexpensive,reproducible as well as to give numerical and accurate results in real *** should be predictive of long term outcomes related with fibrosis and PHT to allow prognostic ***,many types of noninvasive alternative tests have been developed and are under *** particular,imaging and ultrasound based tests,such as transient elastography,have shown promising *** most of these noninvasive tests effectively identify severe fibrosis and PHT,the methods available for diagnosing moderate disease status are still insufficient,and further investigation is essential to predict outcomes and individualize therapy in this field.

关键词： Hepatic fibrosis Portal hypertension Liver biopsy Hepatic venous pressure gradient Non-invasive test Transient elastography