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How old is too old? In vivo engraftment of human peripheral blood stem cells cryopreserved for up to 18 years-implications for clinical transplantation and stability programs

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World Journal of Stem Cells 2020年第5期12卷 359-367页

作者： John Underwood Mahvish Rahim Carijo West Rebecca Britton Elaine Skipworth Vicki Graves Steven Sexton hillary harris Dave Schwering Anthony Sinn Karen E Pollok Kent A Robertson W Scott Goebel Kerry M Hege Departments of Internal Medicine and Pediatrics Indiana University School of MedicineIndianapolisIN 46202United States Department of Pediatrics Division of Pediatric Hematology/OncologyIndiana University School of MedicineIndianapolisIN 46202United States Cellular Therapy Laboratory Indiana University HealthIndianapolisIN 46202United States Department of Pediatrics Herman B Wells Center for Pediatric ResearchIn Vivo Therapeutics Core and Angio Biocore Shared Resource Facilities for the Indiana University Simon Cancer CenterIndiana University School of MedicineIndianapolisIN 46202United States

BACKGROUND Peripheral blood stem cells(PBSC)are commonly cryopreserved awaiting clinical use for hematopoietic stem cell *** term cryopreservation is commonly defined as five years or longer,and limited data exists regarding how long PBSC can be cryopreserved and retain the ability to successfully *** programs,stem cell banks,and regulatory and accrediting agencies interested in product stability would benefit from such ***,we assessed recovery and colony forming ability of PBSC following long-term cryopreservation as well as their ability to engraft in NOD/SCID/IL-2 Rγnull(NSG)*** To investigate the in vivo engraftment potential of long-term cryopreserved PBSC *** PBSC units which were collected and frozen using validated clinical protocols were obtained for research use from the Cellular Therapy Laboratory at Indiana University *** units were thawed in the Cellular Therapy Laboratory using clinical standards of practice,and the pre-freeze and post-thaw characteristics of the units were *** function was assessed using standard colony-forming ***34-selected cells were transplanted into immunodeficient mice to assess stem cell *** Ten PBSC units with mean of 17 years in cryopreservation(range 13.6-18.3 years)demonstrated a mean total cell recovery of 88%±12%(range 68%-110%)and post-thaw viability of 69%±17%(range 34%-86%).BFU-E growth was shown in 9 of 10 units and CFU-GM growth in 7 of 10 units *** mice were transplanted with CD34-selected cells from four randomly chosen PBSC *** mice demonstrated long-term engraftment at 12 wk with mean34%±24%human CD45+cells,and differentiation with presence of human CD19+,CD3+and CD33+*** bone marrow from all mice demonstrated growth of erythroid and myeloid *** We demonstrated engraftment of clinically-collected and thawed PBSC following cryopreservation up to 18 years in NSG mice,sign

关键词： Colony-forming units assay Cryopreservation Hematopoietic stem cells Hematopoietic stem cell transplantation In vitro techniques Peripheral blood stem cell Viability Transplant Long-term storage

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