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Claudin-1 Leads to Strong Formation of Tight Junction in Cultured Mouse Lung Microvascular Endothelial Cells

Pharmacology & Pharmacy 2016年第3期7卷 133-139页

作者： Yukari Ueda Yasuhumi Shinmyouzu hikaru nakayama Tadatoshi Tanino Eiko Sakurai Eiichi Sakurai Department of Pharmaceutics Faculty of Pharmaceutical Sciences Tokushima Bunri University Tokushima Japan Faculty of Pharmacy Iwaki Meisei University Fukushima Japan

We aimed to examine paracellular barrier function in cultured mouse lung microvascular endothelial cells (LMECs). The transcellular resistance of LMEC monolayers yielded an electrical resistance of approximately 19 Ω × cm2 at days 6 - 7 in culture when the cells reached confluence, and paracellular permeable clearance of sodium fluorescein was the lowest on day 6 in culture, suggesting the formation of tight junctions (TJs) in cultured LMECs. Moreover, the expression of TJ-associated proteins, occludin, claudin-1 and -4 and zonula occludents 1 (ZO-1) was detected in LMECs at day 6 in culture. However, mRNAs of occludin, claudin-1 and -4 and ZO-1 were already expressed on day 1 after culture, and large variations were absent in the mRNA levels of occludin, claudin-4 and ZO-1 between days 1 and 7 in culture, when the level of each mRNA on day 1 in culture was used as a basal level. However, the claudin-1 mRNA level gradually increased up to approximately 7-fold on day 7 in culture over the basal level. These results indicate that the drastic increase in the mRNA expression level of claudin-1 leads to the strong formation of TJs.

关键词： Mouse Lung Microvascular Endothelial Cells Paracellular Permeability Tight Junction Occludin Claudins Zonula Occludents

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Translation and Validation of the Japanese Version of the Family Sense of Coherence Scale-Short Form in Nurses

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Open Journal of Nursing 2019年第8期9卷 901-910页

作者： Yuichi nakayama Motoko Hori Tae Kawahara hikaru Sou Akemi Yamazaki Division of Health Sciences Osaka University Graduate School of Medicine Osaka Japan Faculty of Nursing Setsunan University Osaka Japan Faculty of Nursing SenriKinran University Osaka Japan

Purpose: The purpose of this study was to translate the Family Sense of Coherence Scale-short form (FSOC-S) into a Japanese version (J-FSOC-S) for Japanese nurses and to evaluate its reliability and validity. Methods: First, we obtained permission to translate the FSOC-S from the original author and translated it into Japanese using the translation/back translation technique. Second, the J-FSOC-S was reviewed by a panel of experts in nursing research and practice, then, a validation survey was conducted. The internal consistency of the J-FSOC-S was assessed using Cronbach’s α coefficient. Test-retest reliability was examined through intra-class correlation coefficients (ICC). Construct validity was investigated by measuring the correlation coefficients between the J-FSOC-S and Sense of Coherence (SOC) Scale (concurrent validity) and the Family Adaptability Partnership, Growth, Affection, and Resolve Index (Family APGAR) (convergent validity). Results: A total of 374 nurses (valid response rate = 58.6%) were recruited from seven hospitals. Thirty-four participants completed the questionnaire twice at an interval of 2 - 4 weeks to test the reliability. The mean age of the participants was 35.0 years (SD = 7.6, range = 25 - 59). The mean years of experience as a registered nurse were 11.0 years (SD = 7.1, range = 5 - 36). Of the total number of participants, 53.7% had children. The J-FSOC-S showed good internal consistency (Cronbach’s α = 0.85) and test-retest reliability (ICC = 0.77). The J-FSOC-S correlated positively with the SOC Scale (r = 0.41, p Conclusions: The J-FSOC-S is a reliable and valid instrument for measuring family sense of coherence in Japan.

关键词： Family Sense of Coherence Psychometric Testing Nurses

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Translation and Validation of the Japanese Version of the Family Sense of Coherence Scale-Short Form in Nurses

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Open Journal of Nursing（护理学期刊） 2019年第8期

作者： Yuichi nakayama Motoko Hori Tae Kawahara hikaru Sou Akemi Yamazaki Faculty of Nursing SenriKinran University Osaka

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