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汽车与新动力 2005年第1期 45-51页

作者： Nobutaka Hiramatsu hideo ishikawa Takeshi Katagiri 徐启圣

近年来,作为环境问题的一项对策,一直要求柴油机减少废气排放。同时,为了提高燃油效率,发动机的气缸压力也在不断增加,因此发动机轴承,必须在恶劣的高比负载条件下工作。由于连杆轴承,特别是柴油机的连杆轴承在超过100MPa 的高比负载下... 详细信息

近年来,作为环境问题的一项对策,一直要求柴油机减少废气排放。同时,为了提高燃油效率,发动机的气缸压力也在不断增加,因此发动机轴承,必须在恶劣的高比负载条件下工作。由于连杆轴承,特别是柴油机的连杆轴承在超过100MPa 的高比负载下工作,轴承表面会发生弹性变形,在轴承宽度两边缘处的油膜厚度会减少。这就会使轴承与曲轴直接接触,从而导致轴承的磨损,甚至可能引起粘着。引起粘着的因素有:轴承材料、轴承形状、加工方法以及装配不当。针对这些因素,本研究利用试验装置评定了实际发动机的连杆轴承性能,然后将试验结果与按弹性流体润滑理论分析的结果进行了比较。

关键词：柴油机轴承试验研究

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Usefulness of Immuno-Magnetic Beads Conjugated with Anti-EpCAM Antibody for Detecting Endometrial Cancer Cells

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Journal of Cancer Therapy 2013年第8期4卷 1273-1282页

作者： Yoshikatsu Koga Satoshi Katayose Nobuko Onoda Takahiro Kasamatsu Tomoyasu Kato Shunichi Ikeda Mitsuya ishikawa Ken Ishitani Yasuo Hirai hideo Matsui Yasuhiro Matsumura Department of Gynecology National Cancer Center Hospital Tokyo Japan Department of Obstetrics and Gynecology Tokyo Women’s Medical University Tokyo Japan Department of Obstetrics and Gynecology Tokyo Women’s Medical University Tokyo Japan. Division of Developmental Therapeutics Research Center for Innovative Oncology National Cancer Center Hospital East Kashiwa Japan R & D Department Unit 1 JSR Life Sciences Corporation Tsukuba Japan

A simple and non-invasive method for detecting endometrial cancer in women with abnormal uterine bleeding is required. For this purpose, we prepared immuno-magnetic beads conjugated with anti-human EpCAM rat monoclonal antibody (mAb) for isolating exfoliated endometrial cells including endometrial cancer cells in vaginal discharge. The affinities of the anti-human EpCAM rat mAbs were analyzed by flow cytometry and immunocytochemistry and then magnetic beads were conjugated with the mAbs. The rate of retrieval of endometrial cells using the immuno-magnetic beads was calculated. Endometrial cells were isolated using the immuno-magnetic beads from the vaginal discharges of 22 patients with endometrial cancer and 16 non-malignant controls. The isolated cells were stained using endometrial cancer specific-mAbs and analyzed by flow cytometry and imaging cytometry. The immuno-magnetic beads conjugated with high-affinity mAb (clone 1456) appeared to have very low auto-fluorescence. Sufficient enrichment of Ep-CAMpositive cells using immuno-magnetic beads was observed in both simulation and clinical samples. The overall sensitivities of flow cytometry and imaging cytometry to detect endometrial cancer cells were 72.7% and 45.5%, respectively. Meanwhile, the overall specificities of flow cytometry and imaging cytometry for healthy controls were 75.0% and 81.3%, respectively. Our immuno-magnetic beads have very low auto-fluorescence, so they could be useful for fluorescent analysis, such as fluorescent immunochemical staining. In the future, these novel immuno-magnetic beads could be used for cytological study.

关键词： Immuno-Magnetic Beads Auto-Fluorescence Endometrial Cancer Cancer Screening

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Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility

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Journal of Animal Science and Biotechnology 2020年第1期11卷 44-57页

作者： MariaPortia B.Nagata Junki Egashira Naoto Katafuchi Kenji Endo Kazuko Ogata Kenichi Yamanaka Tadayuki Yamanouchi hideo Matsuda Yutaka Hashiyada Kenichi Yamashita Advanced Manufacturing Research Institute National Institute of Advanced Industrial Science and Technology(AIST)807-1 Shuku-machiTosuSaga 841-0052Japan Saga Prefectural Livestock Experiment Station 23242-2 Yamauchi-machiMiyanoTakeoSaga 849-2305Japan Morinaga Dairy Service Co.Ltd. 1-159 ToyoharaotsuNasu-gun Nasu-machiTochigi 329-3224Japan National Livestock Breeding Center(NLBC) 1 OdakuraharaOdakuraNishigo-muraNishishirakawa-gunFukushima 961-8511Japan Institute of Livestock and Grassland Science National Agriculture and Food Research Organization(NARO)Ikenodai 2TsukubaIbaraki 305-0901Japan Faculty of Agriculture Saga University1 Honjo-machiSaga 840-8502Japan.7Ishikawa Prefectural University1-308 SuematsuNonoichi-shiIshikawa 921-8836Japan.

Background:The application of cryopreservation and artificial insemination technology have contributed to the advancement of animal ***,a substantial proportion of spermatozoa undergoes alterations and loses their fertility during cryopreservation,rendering the frozen-thawed semen impractical for routine *** is known to reduce sperm lifespan and *** in cryosurvival of spermatozoa from different sires and even with the individual sire is common in artificial insemination(AI)*** goal is to improve post-thawed semen quality by optimization of cryopreservation technique through sperm selection prior to cryopreservation ***:Our strategy of sperm selection based on rheotaxis and thermotaxis(SSRT)on macrosale in a rotating fluid flow demonstrated the ability to maintain the original pre-freezing structural integrity,viability and biological function related to fertilization *** strategy has a positive effect on the cryosurvival and fertilizing abilities of spermatozoa as supported by the improvement on pregnancy rate of Japanese Black heifers and Holstein repeat *** technique protected further sublethal damage to bovine spermatozoa(higher%cryosurvival than the control)and resulted in the improvement of DNA *** selected spermatozoa demonstrated slower and controlled capacitation than unprocessed control which is thought to be related to sperm longevity and consequently to appropriate timing during in vivo ***:These results provide solid evidence that improvement of post-thawed semen quality by SSRT method is beneficial in terms of cryosurvival,longevity of post-thawed sperm,and optimization of in vivo fertilization,embryo development and calving as supported by the favorable results of field fertility study.

关键词： Cryopreservation Fertility Livestock Rheotaxis Spermatozoa Thermotaxis

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<i>Lactobacilli</i>Enjoyed Fermented Herbs on to the Last Fragment and Regulated Leucocyte Subsets and Anti-Oxidative Activity

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Open Journal of Rheumatology and Autoimmune Diseases 2017年第1期7卷 30-45页

作者： Nobuo Yamaguchi Yoshiichiroh Mastuba Kazuhiro Okamoto Daisuke Sakamoto Takanao Ueyama hideo Matsuno Nurmuhamamt Amat ishikawa Natural Medicinal Products Research Center Ishikawa Japan Department of Rehabilitation Kanazawa Medical University Uchinada-Machi Japan Department of Heart Surgery Kanazawa Medical University Himi Municipal Hospital Himi Japan Department of 2nd Internal Medicine Kansai Medical University Osaka Japan Department of Otolaryngology Kanazawa Medical University Uchinada-Machi Japan Traditional Uighur Medicine Department Xinjiang Medical University Urumqi China

Eighty sorts of herbs were fermented by Lactobaccilli (f-ESH). This material was proved by as safe in animal safety experiment. This work was aimed to revise the traditional way of hot water extraction to the fermentation, in order to use up the original material and searching new activity as well. We tried to prove the new activity by fermentation for immune-competent cells in the host that administrated both of original remedy and the new fermented sample. In rodents, immune-compromised host was prepared with cancer chemotherapeutic agent. Our observations showed that the antigen stimulated animal increased the antibody producing cell for heterologous erythrocyte by compromised host by f-ESH. Moreover, by administration of f-ESH to immno-suppressed hosts, these samples regulated lymphocyte in number and functions, macrophage activities, and regulating anti-oxidative activity by phagocytic cells. The anti-oxidative assay was carried out ex-vivo system by peritoneal excaudate cell that we propose as suitable system for evaluating antioxidative assay. With these evidences, the original COF only augmented the level of lymphocytes in number, while f-ESH regulated the ratio of granulocytes and lymphocyte. In clinical study with 20 healthy volunteers, granulocyte and lymphocyte ratio was also resulted as neutral in peripheral white blood cells, 1, 2 and 3 weeks after the administration of f-ESH. We discussed the significance and mechanism of cleving complement components Fb by f-ESH in this text. Fb. The new cleavage of complement was directly evident by immune-electrophoretic method. In conclusion, this modification of food materials introduced new style of food intake resulted in the significant regulation such as antibody producing cells and anti-oxidative activity for phagocytic cells comparing traditional processing by hot water extraction.

关键词： Wild Herbs Fermentation Lactobacilli Lymphocyte Granulocyte Ant-Oxidative Activity GABA Complement Alternative Complement Activation

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水的净化和供给设备的监测和控制网络系统

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水处理信息报导 2005年第6期 46-46页

作者： ishikawa hideo 刘国晶（译） Wealthy K．K． Japan 不详

这种监测通过沿净化管线收集数据，累积在一个数据文档服务器内完成。根据计算机网络系统优化操作对设备进行控制。

关键词：控制网络系统监测设备净化计算机网络系统供给数据文档优化操作水服务器

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