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In vitro antibacterial activity of the Hertia cheirifolia *** oils

Journal of Coastal Life Medicine 2016年第11期4卷 865-867页

作者： Kaouther Majouli Malek Besbes Hlila Guido Flamini hichem ben Jannet Abderraouf Kenani Laboratory of Biochemistry:Research Unit 12ES08 Cell Signaling and PathologiesFaculty of MedicineUniversity of MonastirTunisia Laboratory of Transmissible Diseases and Biologically Active Substances MDT01Faculty of PharmacyUniversity of MonastirTunisia Department of Pharmacy University of PisaStreet Bonanno 656126 PisaItaly Laboratory of Heterocyclic Chemistry Natural Products and Reactivity Team:Medicinal Chemistry and Natural Products(LR11ES39)Faculty of SciencesUniversity of MonastirTunisia

Objective:To investigate the antibacterial activity of essential oils from Hertia cheirifolia ***,vegetative part(leaves+stems)and roots against a panel of bacterial ***:The essential oils from Hertia cheirifolia ***,vegetative part(leaves+stems)and roots were analyzed by gas chromatography and gas chromatography-mass *** antibacterial activity of essential oils was evaluated in vitro by the broth dilution ***:The results showed that the essential oil of flowers exhibited a strong antibacterial activity against Staphylococcus aureus with minimal inhibitory concentration of 0.078 mg/***:The essential oils of Hertia cheirifolia can be a good source of antibacterial *** can be used for pharmaceutical treatments.

关键词： Essential oil Chemical composition Antibacterial activity Hertia cheirifolia L.

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Total phenolic compounds,antioxidant potential and α-glucosidase inhibition by Tunisian Euphorbia paralias L.

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Journal of Coastal Life Medicine 2016年第8期4卷 628-633页

作者： Malek Besbes Hlila Kaouther Majouli Fethia Harzallah Skhiri hichem ben Jannet Mahjoub Aouni Maha Mastouri Boulbaba Selmi Laboratory of Transmissible Diseases and Biological Active Substances Faculty of PharmacyUniversity of MonastirAvenue Avicenne5000 MonastirTunisia Biochemistry Laboratory:Cell Signaling and Pathologies Research Unit:UR 12ES08Faculty of Medicine of MonastirTunisia Laboratory of Genetic Biodiversity and Valorisation of BioresourcesLR11ES41Higher Institute of Biotechnology of MonastirUniversity of MonastirMonastir 5000Tunisia Laboratory of Heterocyclic Chemistry Natural Products and ReactivityTeam:Medicinal Chemistry and Natural ProductsFaculty of Sciences of MonastirUniversity of MonastirMonastirTunisia Laboratory of Bioresources:Integrative Biology and exploiting Higher Institute of Biotechnology of MonastirMonastirTunisia

Objective:To examine the potential antioxidant and anti-α-glucosidase inhibitory activities of Tunisian Euphorbia paralias *** and stems extracts and their composition of total polyphenol and ***:The different samples were tested for their antiradical activities by using 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)and 1,1-diphenyl-2-picrylhydrazyl(DPPH)***α-glucosidase activity,α-glucosidase(0.3 IU/mL)and substrate,2500μmol/L p-nitrophenylα-D-glucopyranoside were used;absorbance was registered at 405 ***:The leaves acetonic extract exhibited the strongestα-glucosidase inhibition[IC_(50)=(0.0035±0.001)μg/mL],which was 20-fold more active than the standard product(acarbose)[IC_(50)=(0.07±0.01)μg/mL].Acetonic extract of the leaves exhibited the highest quantity of total phenolic[(95.54±0.04)μg gallic acid equivalent/mg]and flavonoid[(55.16±0.25)μg quercetin equivalent/mg].The obtained findings presented also that this extract was detected with best antioxidant capacity[IC_(50)=(0.015±0.01)μg/mL]against DPPH and a value of IC_(50)equal to(0.02±0.01)μg/mL against *** relationship between polyphenolic content of the tested Euphorbia paralias *** and stems extracts and its antioxidant activity(DPPH and ABTS)was *** positive linear correlation was got between ABTS and total phenolic(R^(2)=0.751).Conclusions:The findings clearly demonstrate that the use of a polar solvent enables extraction of significant quantities of phenol compounds and flavonoids.

关键词： Euphorbia paralias L. Polyphenolic compounds Antioxidant activity Anti-α-glucosidase activity

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