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Molecular cloning and characterization of the endothelin 3 gene in black bone sheep

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Journal of Animal Science and Biotechnology 2018年第4期9卷 847-857页

作者： hesham Y.A.darwish Yuanyuan Zhang Kai Cui Zu Yang Deping Han Xianggui Dong Huaming Mao Weidong Deng Xuemei Deng National Engineering Laboratory for Animal Breeding and Key Laboratory of Animal Genetics Breeding and Reproduction of the Ministry of AgricultureChina Agricultural University Animal Production Research Institute Agricultural Research Center Ministry of Agriculture and Land Reclamation College of Veterinary Medicine China Agricultural University College of Animal Science and Technology Yunnan Agricultural University

Background: Black bone sheep was first discovered in Yunnan province of China in 1970, with unique black pigmentation on the body and internal organs. Endothelin 3(EDN3) has been known as a key gene causing hyperpigmentation in black bone chicken, the Silky ***: In this study, EDN3 was employed as a candidate gene for regulating black color pigmentation. First,EDN3 was cloned from sheep to obtain the full-length cDNA by using the rapid amplification of cDNA ends(RACE).Genomic EDN3 was screened and a total of thirty predicted single nucleotide polymorphisms(SNPs) were genotyped for allele and genotype frequency analysis in a case-control study involving two black bone sheep populations. Genomic copy number analysis of EDN3 in sheep was conducted to measure the variation in copy number. EDN3 expression levels were observed among the groups in adult liver, lymph node, and kidney tissues, as well as embryo kidney samples. Also, among the tissues of black bone and non-black bone ***: The size of the full-length cDNA was 1,578 bp, which included 426 bp of 5′-untranslated region(5′-UTR),an open reading frame(ORF) of 639 bp encoding a protein of 212 amino acids, and a 3′-UTR of 513 bp. Genotype and allele frequencies of all the discovered SNPs were found insignificantly different in black bone and non-black bone sheep(P > 0.05). Genomic copy number analysis of EDN3 in sheep revealed no significant difference between the two sheep groups. No significant variations were found in the adult liver and kidney embryo samples. However,the expression in lymph node and kidney tissue was significantly higher in black bone sheep than that in non-black bone sheep(P < 0.05). Significant variations in the EDN3 expression levels were observed among the tissues of nonblack bone ***: The findings of the present study indicate that unlike in Silky chickens, EDN3 is not responsible for hyperpigmentation but may play a key functional role in immune and excretory

关键词： Black pigmentation Expression level Sequence analysis SNP

Utility-Based Anonymization Using Generalization Boundaries to Protect Sensitive Attributes

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Journal of Information Security 2015年第3期6卷 179-196页

作者： Abou-el-ela Abdou Hussien Nagy Ramadan darwish hesham A. Hefny Department of Computer Science Faculty of Science and Arts Shaqra University Shaqra KSA Department of Computer and Information Sciences Institute of Statistical Studies and Research Cairo University Cairo Egypt

Privacy preserving data mining (PPDM) has become more and more important because it allows sharing of privacy sensitive data for analytical purposes. A big number of privacy techniques were developed most of which used the k-anonymity property which have many shortcomings, so other privacy techniques were introduced (l-diversity, p-sensitive k-anonymity, (α, k)-anonymity, t-closeness, etc.). While they are different in their methods and quality of their results, they all focus first on masking the data, and then protecting the quality of the data. This paper is concerned with providing an enhanced privacy technique that combines some anonymity techniques to maintain both privacy and data utility by considering the sensitivity values of attributes in queries using sensitivity weights which determine taking in account utility-based anonymization and then only queries having sensitive attributes whose values exceed threshold are to be changed using generalization boundaries. The threshold value is calculated depending on the different weights assigned to individual attributes which take into account the utility of each attribute and those particular attributes whose total weights exceed the threshold values is changed using generalization boundaries and the other queries can be directly published. Experiment results using UT dallas anonymization toolbox on real data set adult database from the UC machine learning repository show that although the proposed technique preserves privacy, it also can maintain the utility of the publishing data.

关键词： Privacy Privacy Preserving Data Mining K-Anonymity Generalization Boundaries Suppression

Vulnerability to Desertification in Lebanon Based on Geo-information and Socioeconomic Conditions

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Journal of Environmental Science and Engineering(B) 2012年第7期1卷 851-864页

作者： Talal darwish Pandi Zdruli Ramy Saliba Mohamad awad Amin Shaban Ghaleb Faour National Center for Remote Sensing National Council for Scientific Research (CNRS) Beirut 11072260 Lebanon Land and Water Resources Management Department Mediterranean Agronomic Institute of Bari Valenzano 70010 Italy

Desertification caused by land degradation and overexploitation of natural resources is threatening large parts of eastern and southern Mediterranean. The actual state of desertification sensitivity in Lebanon was spatially assessed using site specific environmental bio-physical indicators, demographic pressure and socioeconomic conditions. Bio-physical assessment included the aridity index derived from integrated assessment of the historical data for 48 climatic stations spread throughout the country, the new detailed soil map at 1：50,000 scale, and the updated land cover/use map at 1：20,000 derived from IKONOS 2005. The methodology also included livelihood conditions and poverty at local administrative ＂Caza＂ level. Results showed the integrated impact of local climate, soil and vegetation quality and socioeconomic conditions on sensitivity to desertification. A total of 78% of the territories have low and very low climate quality index preconditioning the sensitivity to desertification. Fourteen Cazas out of 26 in total, representing more than 66% of the country, have low socioeconomic satisfaction index. Furthermore, negative trends are alleviated by good quality relict soils and vegetation cover. The actual extent of desertification covers 40.48% of the national territory, much of which occurs under semi-arid climate, moderate or low soil and vegetation quality and poor living conditions. The outcome of this research adjusted the previous coarse estimates of desertification prone areas at the national level. Results allow for realistic, policy oriented local assessment for responsive land use planning and proactive sustainable, national and local land management in the context of the national action plan to combat desertification.

关键词： Integrated assessment land degradation east Mediterranean sensitivity sustainable land management.

Application of Plant Growth-Promoting Bacteria as an Eco-Friendly Strategy for Mitigating the Harmful Effects of Abiotic Stress on Plants

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Phyton-International Journal of Experimental Botany 2023年第12期92卷 3305-3321页

作者： Ahmed Hassan Abdou Omar Abdullah Alkhateeb Hossam Eldin Hamed Mansour hesham S.Ghazzawy Muayad Saud Albadrani Nadi awad Al-harbi Wasimah B.Al-Shammari Khaled Abdelaal Social Studies Department College of ArtsKing Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Agribusiness and Consumer Sciences College of Agriculture&Food SciencesKing Faisal UniversityAl-Ahsa31982Saudi Arabia Agribusiness and Consumer Sciences Department College of Agricultural and Food SciencesKing Faisal UniversityAl-Ahsa31982Saudi Arabia Date Palm Research Center of Excellence King Faisal UniversityAl-Ahsa31982Saudi Arabia Department of Family and Community Medicine College of MedicineTaibah UniversityAl-Madinah Al-Munawara42353Saudi Arabia Biology Department University College of TaymaUniversity of TabukTabukSaudi Arabia Department of Biology College of ScienceUniversity of HailP.O.Box 2440Hail55476Saudi Arabia EPCRS Excellence Center Plant Pathology and Biotechnology LaboratoryAgricultural Botany DepartmentFaculty of AgricultureKafrelsheikh University33516Egypt

Plant growth-promoting bacteria(PGPB)play an important role in improving agricultural production under several abiotic stress *** can be used to increase crop growth and development through hormonal balance and increase nutrient *** positive effect of PGPB may be due to its pivotal role in morphophysiological and biochemical characteristics like leaf number,leaf area,and stem ***,relative water content,chlorophyll content,carotenoids,antioxidant enzymes,and plant hormones were improved with PGPB *** yield and yield components were also increased with PGPB treatment in numerous *** anatomical structure of plant organs was increased such as lamina thickness,stem diameter,xylem vessel diameter,and number of xylem vessels as well as phloem thickness under treatment with ***,PGPB can alleviate the negative effects of several abiotic stresses by regulating the antioxidant defense system to scavenge the reactive oxygen species resulting in an improvement of yield production in the stressed ***,gene expressions were controlled by calcium ion modulation during secondary messengers that act upon calcium-dependent protein kinase and protein *** includes many transcription factors such as MYB,AP2/ERF,bZIP,and NAC which regulate genes related to salinity stress *** can demonstrate induction genes of signaling under abiotic stress *** review gives an outline of the PGPB role in alleviating the harmful effects of abiotic factors such as salinity,drought,and heat associated with the improvement of the morpho-physiological and biochemical features especially,leaves and branches number,leaf area,antioxidant compounds,plant hormones,and relative water content.

关键词： PGPB salinity drought heat reactive oxygen species antioxidants gene expressions

Soil Forest Ecosystem and Forest Fire Effect on Soil Organic Carbon Stock in Lebanon

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Journal of Life Sciences 2018年第1期12卷 47-58页

作者： darwish Talal Abou Daher Mohamad Jomaa Ihab Faour Ghaleb awad Mohamad Hamze Mouin National Council for Scientific Research Centre for Remote Sensing Beirut 8281/11 Lebanon Head of Educational Department/MsC Ministry of Agriculture Beirut 8281/11 Lebanon Lebanese Agricultural Research Institute Tal Amara Bekaa Zahle 287 Lebanon National Council for Scientific Research Beirut 8281/11 Lebanon

Accurately quantifying SOC （soil organic carbon） store in soils is considered fundamental to global climate change modeling, notably in soil forest ecosystems in relation to land cover/use change, forest fires and sustainable land management practices. Lebanese soils were described as relatively poor in organic matter content. This work used Landsat and IRS （Indian Remote Sensing） to classify land cover and land use, field survey to map the soil cover and assess carbon stock in the Lebanese soil and forest ecosystems and link the frequency of forest fires with the soil nature and land cover land use to assess the resulting risk of SOC loss from the soil pool. SOC density and stock was assessed using information from 450 soil profiles and the average OC content in a given soil type was calculated involving organic matter content in the soil, bulk density, soil thickness and the content of coarse fragments to produce the national SOC stock map. A total of 11 soil types （major classes） represented the mapping units describing the higher soil component or major classes were identified in Lebanon. The higher standard deviation observed in Luvisols reflects soil diversity, history and different management. Comparing the results of surface soil layer with the overall SOC density and stock in Lebanese soils showed a good correlation （0.98） between the OC stock within the upper soil layer and in the 100 cm depth, indicating the absolute role of the upper, biologically most active soil layer in the organic matter accumulation and carbon sequestration. Observation over a pilot area in the High Meten showed the calcareous soil group consists of Luvisols with associated Cambisols. Soil units representing the transition between the two soil groups are markedly affected by erosion-deposition processes. Pine occupies the largest area of forest （3,600 ha） followed by oak （1,800 ha） and mixed forest （1,200 ha）, representing 24.9%, 12.5 % and 8.2% of forest cover respectively. The ana

关键词： Land cover mountain pedogenesis soil organic matter east mediterranean fires drought.

Genome-wide profiling of RNA editing sites in sheep

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Journal of Animal Science and Biotechnology 2019年第2期10卷 274-282页

作者： Yuanyuan Zhang Deping Han Xianggui Dong Jiankui Wang Jianfei Chen Yanzhu Yao hesham Y.A.darwish Wansheng Liu Xuemei Deng Key Laboratory of Animal Genetics Breeding and Reproduction of the Ministry of Agriculture&Beijing Key Laboratory of Animal Genetic ImprovementChina Agricultural UniversityBeijing 100193China Department of Animal Science Pennsylvania State UniversityUniversity ParkPA 16802USA.3Animal Production Research InstituteAgricultural Research CenterMinistry of Agriculture and Land ReclamationGiza 12618Egypt.

Background: The widely observed RNA-DNA differences(RDDs) have been found to be due to nucleotide alteration by RNA editing. Canonical RNA editing(i.e., A-to-I and C-to-U editing) mediated by the adenosine deaminases acting on RNA(ADAR) family and apolipoprotein B mRNA editing catalytic polypeptide-like(APOBEC)family during the transcriptional process is considered common and essential for the development of an *** date, an increasing number of RNA editing sites have been reported in human, rodents, and some farm animals;however, genome-wide detection of RNA editing events in sheep has not been reported. The aim of this study was to identify RNA editing events in sheep by comparing the RNA-seq and DNA-seq data from three biological replicates of the kidney and spleen ***: A total of 607 and 994 common edited sites within the three biological replicates were identified in the ovine kidney and spleen, respectively. Many of the RDDs were specific to an individual. The RNA editing-related genes identified in the present study might be evolved for specific biological functions in sheep, such as structural constituent of the cytoskeleton and microtubule-based processes. Furthermore, the edited sites found in the ovine BLCAP and NEIL1 genes are in line with those in previous reports on the porcine and human homologs, suggesting the existence of evolutionarily conserved RNA editing sites and they may play an important role in the structure and function of ***: Our study is the first to investigate RNA editing events in sheep. We screened out 607 and 994 RNA editing sites in three biological replicates of the ovine kidney and spleen and annotated 164 and 247 genes in the kidney and spleen, respectively. The gene function and conservation analysis of these RNA editing-related genes suggest that RNA editing is associated with important gene function in sheep. The putative functionally important RNA editing sites reported in the present study

关键词： DNA resequencing RNA-DNA differences RNA editing RNA-seq Sheep

Performance Evaluation of One-Way Concrete Slabs Reinforced with New Developed GFRP Bars

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Materials Sciences and Applications 2015年第5期6卷 420-435页

作者： Ahmed H. Ali Mohammad Z. Afifi Bahira Abdulsalam hesham Haggag awad El Hashimy Tarek El-Sayed Hamdy M. Mohamed Department of Civil Engineering Helwan University Cairo Egypt Department of Civil and Environmental Engineering Western University London Canada

The incorporation of fiber-reinforced-polymer (FRP) bars in construction as a replacement to steel bars provides a superior material which is capable to overcome corrosion problems. However, serviceability requirements are important issues to be considered in the design of concrete elements reinforced with glass-FRP (GFRP) bars which are known to have larger deflections and wider crack widths as well as weaker bond compared with steel reinforced concrete. As a solution to this problem, square GFRP bars are proposed. This paper presents the results of an experimental investigation that was performed, in which newly developed square and circular GFRP bars were fabricated in the lab. Also, the GFRP bars were tested and used to reinforce concrete slabs. A total of nine full-scale GFRP-reinforced concrete (RC) one-way slabs were constructed, tested and analyzed, considering the most influencing parameters such as the cross sectional shape of GFRP bars, reinforcement ratio, the concrete characteristics strength, and adding polypropylene fibers to the concrete mixture. The test results were showed that, the tested slabs with GFRP square bars improved the deflection and cracking behavior as well as the ultimate load.

关键词： Square and Circular Bars GFRP Reinforcement Bars Polypropylene Fibers Concrete Slabs