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Histopathological and molecular study of Neospora caninum infection in bovine aborted fetuses

Asian Pacific Journal of Tropical Biomedicine 2014年第12期4卷 990-994页

作者： Amir Kamali hesam adin seifi Ahmad Reza Movassaghi Gholam Reza Razmi Zahra Naseri Department of Clinical Sciences Faculty of Veterinary MedicineFerdowsi University of Mashhad Center of Excellence in Ruminant Abortion and Neonatal Mortality Ferdowsi University of Mashhad Department of Pathobiology Faculty of Veterinary MedicineFerdowsi University of Mashhad

Objective:To estimate the extent to which abortion in dairy cows was associated with of Neospom caninum(***) and to determine the risk factors of neosporosis in dairy farms from 9 provinces in ***:Polymerase chain reaction(PCR) test was used to detect Neospora infection in the brain of 395 bovine aborted fetuses from 9 provinces of *** addition,the brains of aborted fetuses were taken for histopathological *** identify the risk factors associated with neosporosis,data analysis was performed by ***:*** was detected in 179(45%) out of 395 fetal brain samples of bovine aborted fetuses using *** the PCR-positive brain samples,only 56 samples were suited for histopathological *** characteristic lesions of Neospora infection including non-suppurative encephalitis were found in 16(28%) of PCR-positive *** risk factors including season,parity of dam,history of bovine virus diarrhea and infectious bovine rhinotracheitis infection in herd,cow's milk production,herd size and fetal appearance did not show association with the *** study showed that Neospora caused abortion was significantly more in the second trimester of pregnancy than other *** addition,a significant association was observed between Neospora infection and ***:The results showed *** infection was detected in high percentage of aborted *** addition,at least one fourth of abortions caused by Neospora *** results indicate increasing number of abortions associated with the protozoa more than reported before in Iran.

关键词： Neospora caninum Dairy herds Abortion PCR Histopathology Risk factors

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Bovine salmonellosis in Northeast of Iran:Frequency,genetic fingerprinting and antimicrobial resistance patterns of Salmonella spp.

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Asian Pacific Journal of Tropical Biomedicine 2014年第1期4卷 1-7页

作者： Hessam A.Halimi hesam A.seifi Mehrnaz Rad Center of Excellence in Ruminant Abortion and Neonatal Mortality and Department of Clinical Sciences School of Veterinary MedicineFerdowsi Unversity of Mashhad Department of Pathobiology School of Veterinary MedicineFerdowsi Unversity of Mashhad

Objective:To evaluate serovar and antimicrobial resistance patterns of Salmonella spp isolated from healthy,diseased and necropsied cows and calves in this observational ***:Nineteen isolates recovered from feces and tissues of salmonellosis-affected animals of two commercial farms in north-east of *** second part of the study,the two farms were sampled 4 times with an interval of 2 *** samples included calves'feces,adult cows'feces,feeds,water,milk filters,and milk fed to *** Salmonella were isolated from 332fecal samples collected from calves and peri-parturient *** Salmonella was recovered from water,feed,milk filers and milk fed to ***:Salmonella Typhimurium was the most frequently isolate among all *** was only accounted for 8%(two out of 24)of *** Salmonella strains were used for the ERIC PCR DNA fingerprinting *** results grouped Salmonella isolates into 3 clusters,suggesting that specific genotypes were responsible for each sero-group of *** results also revealed diversity among Salmonella isolates in cluster III(sero-group B).Eighteen out of 19 Salmonella *** resistant to *** isolates out of 19 showed more than one drug ***-drug resistance was seen only among Salmonella Typhimurium *** was the most susceptible antibiotic against all isolates in this ***:The emergence of multiple antibiotic-resistant strains of Salmonella Typhimurium should be of great concern to the *** correlation between ERIC fingerprinting and resistance patterns of Salmonella isolates was found,which indicates resistance to antimicrobial agents was not related to specific genetic background.

关键词： Dairy cattle Salmonella Typhimurium Antibiotic resistance ERIC PCR

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