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Effects of Heat Stress during Seed Filling Stage on Brassica napus Seed Oil Accumulation and Chlorophyll Fluorescence Characteristics

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 333-348页

作者： Ruizhi Huang Huasheng Yu Yong Yang heqin liu Xuelong Wu Zhihong liu Haiyan He Gengwei Wu Wengjia Wang Hua Wang State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Institute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhou310021China Institute of Crop Science and Nuclear Technology Utilization Zhejiang Academy of Agricultural SciencesHangzhou310021China

As global temperature rise,the threat of heat stress to rapeseed production is becoming more *** the response characteristics of two important biological pathways,oil accumulation and photosynthesis,to heat stress during *** seed filling is helpful in the genetic improvement of heat-tolerant *** effects of heat stress on seed oil accumulation and chlorophyll fluorescence characteristics of 29 *** germplasms with different oil content and environmental sensitivity,including 6 rapeseed varieties which exhibited environmentsensitive/insensitive and with high,medium or low oil content,were tested by whole plant heat stress or the in vitro silique culture *** assay exhibited similar trend on oil content of the rapeseed *** heat effect on the chlorophyll fluorescence kinetic parameters F_(v)/F_(m),ETR and Y(Ⅱ)were also *** stress significantly decreased oil content,although there was abundant genetic variation on heat tolerance among the *** analysis showed that the decrease rate of F_(v)/F_(m) of silique heat-stressed *** developing seed was positive correlative to the decrease rate of mature seed oil content of the whole plant heat-stressed rapeseed(R=0.9214,P-value<0.01).Overall,the results indicated that heat stress inhibited oil accumulation and photosynthesis in *** developing *** decrease rate of chlorophyll fluorescence parameter F_(v)/F_(m) of heat-stressed developing seed could be used as the index of heat tolerant rapeseed ***,two heat insensitive rapeseed varieties with high oil content were identified.

关键词： Brassica napus L. heat stress seed filling stage oil accumulation chlorophyll fluorescence characteristics

OspTAC2 encodes a pentatricopeptide repeat protein and regulates rice chloroplast development

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Journal of Genetics and Genomics 2016年第10期43卷 601-608页

作者： Dekai Wang heqin liu Guowei Zhai Liangsheng Wang Jianfeng Shao Yuezhi Tao Institute of Crop and Nuclear Technology Utilization Zhejiang Academy of Agricultural Sciences State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control Zhejiang Academy of Agricultural Sciences Boyce Thompson Institute for Plant Research

Functional chloroplast generation depends on the precise coordination of gene expression between the plastid and the nucleus and is essential for plant growth and development. In this study, a rice（Oryza sativa） mutant that exhibited albino and seedling-lethal phenotypes was isolated from a60Co-irradiated rice population. The mutant gene was identified as an ortholog of the Arabidopsis plastid transcriptionally active chromosome protein 2（p TAC2） gene, and the mutant strain was designated osptac2. Sequence and transcription analyses showed that Osp TAC2 encodes a putative chloroplast protein consisting of 10 pentratricopeptide repeat（PPR） domains and a C-terminal small Mut S-related（SMR） domain. Cytological observations via microscopy showed that the Osp TAC2-green fluorescent fusion protein is localized in the chloroplasts. Transmission electron microscopy revealed that the chloroplast of the osptac2 mutant lacks an organized thylakoid membrane. The transcript levels of all investigated PEP（plastid-encoded RNA polymerase）-dependent genes were dramatically reduced in the osptac2 mutant, whereas the transcript levels of NEP（nuclear-encoded polymerase）-dependent genes were increased. These results suggest that Osp TAC2 plays a critical role in chloroplast development and indicate that the molecular function of the Osp TAC2 gene is conserved in rice and Arabidopsis.

关键词： Oryza sativa Pentatricopeptide repeat protein Chloroplast development

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分析生活饮用水水质检测的重要性

环境与发展 2019年第10期31卷 166-166,168页

作者：刘贺琴厦门百霖净水科技有限公司

饮用水水质检测问题,事关全民生活质量与安全。城市供水系统中生活饮用水水质的检测已经不能够应对日益复杂的环境因素。文章就影响城市水质质量以及饮用水二次净化等进行分析与探讨。然后结合水质检测中各个阶段,对生活饮用水质检测的... 详细信息

饮用水水质检测问题,事关全民生活质量与安全。城市供水系统中生活饮用水水质的检测已经不能够应对日益复杂的环境因素。文章就影响城市水质质量以及饮用水二次净化等进行分析与探讨。然后结合水质检测中各个阶段,对生活饮用水质检测的重要性进行论证,为生活用水检测工作提出相关要求与改善议案。

关键词：生活饮用水饮用水净化水质监测

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植物光呼吸分子机制研究进展

中国农学通报 2011年第3期27卷 232-236页

作者：金怡刘合芹汪得凯陶跃之浙江师范大学化学与生命科学学院浙江金华321004 浙江省农业科学院作物与核技术利用研究所杭州310021

光呼吸是植物的绿色细胞在光照下吸收O2释放CO2的反应,光呼吸强弱是影响C3和C4植物光合效率高低的重要因素。对光呼吸的分子机理研究是光合作用研究的热点之一。本文就光呼吸的代谢途径、光呼吸基因表达调控及光呼吸突变体的筛选等方面... 详细信息

光呼吸是植物的绿色细胞在光照下吸收O2释放CO2的反应,光呼吸强弱是影响C3和C4植物光合效率高低的重要因素。对光呼吸的分子机理研究是光合作用研究的热点之一。本文就光呼吸的代谢途径、光呼吸基因表达调控及光呼吸突变体的筛选等方面的研究进展进行了阐述,对光呼吸的生物学功能进行了探讨,并对光呼吸领域的发展进行了展望。

关键词：光呼吸基因突变体生物学功能

极端温度对籼粳稻苗期光合特性及生理生化特性的影响

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浙江师范大学学报（自然科学版） 2012年第3期35卷 330-337页

作者：冯世座刘合芹浙江师范大学化学与生命科学学院浙江金华321004 浙江省农业科学院作物与核技术利用研究所浙江杭州310021

以籼稻9311和粳稻日本晴为材料,研究了极端温度胁迫下和恢复期材料苗期的光合特性、保护性酶活性及其他生理生化特性的变化.实验结果表明:极端温度胁迫使2个材料叶片的净光合速率急剧降低,但是高温胁迫后9311净光合速率的恢复速度明显... 详细信息

以籼稻9311和粳稻日本晴为材料,研究了极端温度胁迫下和恢复期材料苗期的光合特性、保护性酶活性及其他生理生化特性的变化.实验结果表明:极端温度胁迫使2个材料叶片的净光合速率急剧降低,但是高温胁迫后9311净光合速率的恢复速度明显高于日本晴,后者在低温处理后恢复较快.极端低温使9311光系统Ⅱ的最大光化学效率(Fv/Fm)降低,而对日本晴影响很小,两者均在处理后1 d完全恢复;极端高温处理对于光系统Ⅱ的伤害更为严重,Fv/Fm持续下降直到恢复期第1天,到处理后第4天才恢复到正常状态,与日本晴相比,高温处理使9311的Fv/Fm下降的程度更大,但其恢复也快.极端温度胁迫下叶片叶绿素含量和植株水分含量都下降,丙二醛含量上升,保护性酶超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性升高;总抗氧化能力受极端温度胁迫而降低,在恢复期升高;低温胁迫时日本晴的SOD和CAT活性、总抗氧化能力高于9311,高温处理下则相反.上述结果表明叶片的光合速率、叶绿素荧光参数、保护性酶活性均可作为水稻耐温度胁迫的筛选指标,但是光合速率宜在恢复期测定,而叶绿素荧光参数在处理时测定较为有效.

关键词：极端温度水稻光合特性生理生化特性

高粱种质资源出苗相关性状与SSR标记的关联分析

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浙江农业学报 2021年第6期33卷 965-973页

作者：刘秀慧邹桂花翟国伟刘合芹郑学强陈合云浙江省农业科学院作物与核技术利用研究所浙江杭州310021

选用54对均匀分布在高粱全基因组的SSR标记对110份高粱种质资源进行检测,研究高粱中胚轴长、胚芽鞘长、根长和百粒重4个出苗相关性状与SSR标记的关联性,以期为选育耐深播的优异高粱资源提供理论支持和材料保障。结果表明,高粱中胚轴长... 详细信息

选用54对均匀分布在高粱全基因组的SSR标记对110份高粱种质资源进行检测,研究高粱中胚轴长、胚芽鞘长、根长和百粒重4个出苗相关性状与SSR标记的关联性,以期为选育耐深播的优异高粱资源提供理论支持和材料保障。结果表明,高粱中胚轴长与根长呈显著(P≤0.05)负相关,与胚芽鞘长呈极显著(P≤0.01)正相关。胚芽鞘长、根长和百粒重3个性状间存在极显著(P≤0.01)正相关;在54个SSR标记位点中,共检测到339个等位基因,平均每个位点检测到6.28个等位基因,平均有效等位基因数为2.67个,Shannon's信息指数平均值为1.1299;结构分析将参试高粱种质主要分为3个亚群;关联分析共检测到9个标记与中胚轴长显著(P≤0.05)相关,单个位点解释的贡献率为11.18%~28.05%。5个标记与胚芽鞘长度显著(P≤0.05)相关,单个位点解释的贡献率为11.22%~18.12%。只有1个标记与根长显著(P≤0.05)相关,另检测到2个标记与百粒重显著相关。

关键词：高粱出苗相关性状 SSR标记关联分析

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产氢菌的分离鉴定及其产氢特性研究

生物技术通报 2009年第10期25卷 194-197页

作者：刘合钦徐莉周翔南付强闫云君华中科技大学生命科学与技术学院分子生物物理教育部重点实验室武汉430074

从河底污泥中分离到一株产氢菌,通过16SrDNA序列和系统发育分析,鉴定为克雷伯氏菌属,命名为Klebsiella ***-3。运用单因子试验考察了摇瓶发酵培养基中碳源、氮源、无机盐等因素对克雷伯氏菌HQ-3发酵产氢的影响,并对其产氢特性做了初步... 详细信息

从河底污泥中分离到一株产氢菌,通过16SrDNA序列和系统发育分析,鉴定为克雷伯氏菌属,命名为Klebsiella ***-3。运用单因子试验考察了摇瓶发酵培养基中碳源、氮源、无机盐等因素对克雷伯氏菌HQ-3发酵产氢的影响,并对其产氢特性做了初步研究。结果表明,克雷伯氏HQ-3在葡萄糖浓度为24g/L,蛋白胨为18g/L,KH2PO4为8g/L时具有较高产氢量,其最佳产氢初始pH为8.0左右。

关键词：生物制氢克雷伯氏菌 16S rDNA 产氢性能

4-三氟甲基-2-乙酰甲基吡啶的合成与表征

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河南科学 2008年第7期26卷 771-772页

作者：闫凤美郭彦春刘贺钦郑州大学化学系

设计了一种合成4-三氟甲基-2-乙酰甲基吡啶的路线,实现了吡啶环上2,4-位取代基的取代与转化,讨论了反应物料配比、催化剂用量、反应预热温度等条件对反应产率的影响.

关键词：取代二氢吡啶 2 4-位取代吡啶衍生物合成表征

Changes over flood season in turbidity maximum zone in a mountainous macrotidal estuary from 1986 to 2020

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Journal of Geographical Sciences 2023年第5期33卷 980-998页

作者： liu Ruiqing CHENG heqin TENG Lizhi FAN Heshan State Key Laboratory of Estuarine and Coastal Research East China Normal UniversityShanghai200241China

The construction of channel regulation projects,reservoirs,and other human activities have led to significant changes in channel geometry and hydrodynamic conditions in mountainous macrotidal ***,their impact on the long-term evolution of the turbidity maximum zone(TMZ)in these estuaries is still ***,the Minjiang Estuary(ME)was selected as the study area and using the Gabor filter and surface suspended sediment concentration(SSSC)data retrieved from GF PMS/WFV and Landsat-TM/ETM+/OLI images in the flood season from 1986 to 2020,the flow direction of Chuanshi Waterway,the spatiotemporal evolution characteristics of TMZ in the ME,and the influence of human activities on these were *** results indicate that during flood tides in the past 35 years,the TMZ was mainly distributed in sections from the Changmen to the Chuanshi and Meihua *** construction of the Shuikou Reservoir caused the SSSC to decrease by 65 mg/L at the Chuanshi Tidal Gauge Station in the *** TMZ in the ME waterway channel notably migrated toward the sea due to the waterway regulation project,with the landward and seaward boundaries moving by 2.5 km and 3 km seaward,*** main distribution area moved from Jinpaimen to the section from Chuanshi Waterway to the mouth of the *** variation characteristics were basically consistent with the annual average TMZ in the flood *** the interactions between nature and human interventions,the flow regime of the ME tended to converge in the flood ***,human activities have significantly impacted the long-term evolution of the TMZ in the ME.

关键词： turbidity maximum zone spatiotemporal evolution flow regime remote sensing large-scale engineering Minjiang Estuary