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j.urnal of Biomaterials and Nanobiotechnology 2021年第3期12卷 34-48页

作者： Seyed Mohsen Latifi Christina Tang henry j. donahue Bone Engineering Science and Technology (BEST) Lab Department of Biomedical Engineering Virginia Commonwealth University Richmond VA USA Department of Chemical and Life Sciences Engineering Virginia Commonwealth University Richmond VA USA

In this study, the effect of silica/calcium phosphate (SiCaP) nanocomposite particles on the properties of a novel chitosan-based thermosensitive hydrogel system was examined. SiCaP nanocomposite powder was fabricated using a sol-gel method and then used to fabricate nanocomposite hydrogels (Ch- β/7.5SiCaP and Ch-β/15SiCaP) including chitosan and β-glycerophosphate (Ch-β) as a matrix. Results revealed that compared to the Ch-β hydrogel without SiCaP, the presence of SiCaP particles in nanocomposite hydrogels maintained pH stability during the sol-gel transition, accelerated the gelation and improved the stiffness of nanocomposite hydrogels. Gelation time at 37℃ was reduced approximately 75% and stiffness was increased approximately 115%. Both of these changes are attributed to chemical and physical interactions of the SiCaP bioactive particles with chitosan. Furthermore, compared to the Ch-β hydrogel, the presence of SiCaP in the Ch-β/7.5SiCaP nanocomposite hydrogel did not affect biocompatibility negatively, but improved osteoblastic cell differentiation. Our studies suggest that these nanocomposite hydrogels may offer an innovative approach to bone regeneration strategies.

关键词： Thermosensitivity Composite Hydrogel Bioactive Particles Rheology Biocompatibility

Polydopamine Coating on Titanium Affects Osteoblastic Differentiation to a Greater Degree than Does Surface Roughness

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Advances in Materials Physics and Chemistry 2020年第12期10卷 339-349页

作者： Seyed Mohsen Latifi Ravi Shankar henry j. donahue Bone Engineering Science and Technology (BEST) Lab Virginia Commonwealth University Richmond USA

Biointerface design can greatly influence cell behavior. Therefore, in this study we examined the effects of three surface characteristics, roughness, chemistry, and wettability, on osteoblastic cell differentiation. We examined osteoblastic differentiation on titanium (Ti) samples with four levels of roughness (average roughness: 148.6 ± 23.1, 42 ± 6.2, 14.3 ± 5.5, 7.2 ± 1.6 nm) with or without a nanolayer coating of polydopamine (PDA). In vitro osteogenic differentiation was evaluated by quantifying alkaline phosphatase (AP) activity of human fetal preosteoblastic (hFOB 1.19) cells. The change in surface chemistry of Ti samples as a result of PDA coating was assessed by XPS analysis and water contact angle measurement. Results demonstrated that PDA treated samples were more hydrophilic, compared to untreated samples, and this was substrate roughness independent. Moreover, with the exception of the substrate with an oriented texture of surface nanotopography (RTi-4), the presence of a PDA nanolayer increased AP activity independent of substrate roughness. Our results suggest that surface chemistry and wettability, induced by a PDA nanolayer coating, had a greater effect on osteoblastic differentiation than did surface roughness.

关键词： Surface Chemistry Roughness Polydopamine Wettability Osteoblastic Differentiation

Mediator complex subunit 12 is a gatekeeper of SARS-CoV-2 infection in breast cancer cells

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Genes & Diseases 2022年第1期9卷 5-8页

作者： Shengj.e Zhang Fabao Liu Peter Halfmann Ryan TBehrens Peng Liu Sean j.cilwain Irene MOng Kristine donahue Yidan Wang Yoshihiro Kawaoka Nathan Sherer Wei Xua McArdle Laboratory for Cancer Research University of Wisconsin-MadisonMadisonWI 53706USA Department of Pathobiological Sciences School of Veterinary MedicineUniversity of WisconsinMadisonWI 53706USA Department of Biostatistics and Medical Informatics School of Medicine and Public HealthUniversity of WisconsinMadisonWI 53706USA Department of Biostatistics and Medical Informatics Carbone Cancer CenterUniversity of WisconsinMadisonWI 53706USA Department of Obstetrics and Gynecology School of Medicine and Public HealthUniversity of WisconsinMadisonWI 53706USA Cancer Hospital of the University of Chinese Academy of Sciences(Zhej.ang Cancer Hospital) Institute of Basic Medicine and Cancer(IBMC)Chinese Academy of SciencesHangzhouZhejiang 310022China

Cancer patients are at increased risk for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection and *** other viruses in the SARS family,SARS-CoV-2 employs two host proteins,angiotensin-converting enzyme 2(ACE2)and transmembrane serine protease 2(TMPRSS2),for viral entry.1 Recent studies showed that many of the host proteins identified as potential targets for developing COVID therapies are dysregulated in cancer,2 prompting us to investigate whether human cancer cells are susceptible to SARS-CoV-2 infection,and whether chemotherapy could modulate a cancer patient's risk for infection.

关键词： infection ACE2 cancer

A targetable pathway to eliminate TRA-1-60^(+)/TRA-1-81^(+)chemoresistant cancer cells

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j.urnal of Molecular Cell Biology 2023年第6期15卷 25-39页

作者： Lei Tan Xiaohua Duan Pratyusha Mutyala Ting Zhou Sadaf Amin Tuo Zhang Brian Herbst Gokce Askan Tomer Itkin Zhaoying Xiang Fabrizio Michelassi Michael D.Lieberman Christine AIacobuzio-donahue Steven DLeach Todd Evans Shuibing Chen Department of Surgery Weill Cornell MedicineNew YorkNY 10065USA Center for Energy Metabolism and Reproduction Institute of Biomedicine and BiotechnologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Center for Genomic Health Weill Cornell MedicineNew YorkNY 10065USA The SKI Stem Cell Research Facility The Center for Stem Cell Biology and Developmental Biology ProgramMemorial Sloan Kettering Cancer CenterNew YorkNY 10065USA Genomic Resource Core Facility Weill Cornell Medical CollegeNew YorkNY 10065USA Rubenstein Center for Pancreatic Cancer Research Memorial Sloan Kettering Cancer CenterNew YorkNY 10065USA Division of Regenerative Medicine Hartman Institute for Therapeutic Organ RegenerationAnsary Stem Cell InstituteDepartment of MedicineWeill Cornell MedicineNew YorkNY 10065USA Dartmouth Cancer Center Darmouth CollegeHanoverNH 03755USA

Chemoresistance is a primary cause of treatment failure in pancreatic *** cell surface markers specifically expressed in chemoresistant cancer cells(CCCs)could facilitate targeted therapies to overcome *** performed an antibody-based screen and found that TRA-1-60 and TRA-1-81,two‘stemness’cell surface markers,are highly enriched in ***-more,TRA-1-60^(+)/TRA-1-81^(+)cells are chemoresistant compared to TRA-1-60^(-)/TRA-1-81^(-)*** profiling identified UGT1A10,shown to be both necessary and sufficient to maintain TRA-1-60/TRA-1-81 expression and *** a high-content chemical screen,we identified Cymarin,which downregulates UGT1A10,eliminates TRA-1-60/TRA-1-81 expression,and increases chemosensitivity both in vitro and in ***,TRA-1-60/TRA-1-81 expression is highly specific in primary cancer tissue and positively correlated with chemoresistance and short survival,which highlights their potentiality for targeted ***,we discovered a novel CCC surface marker regulated by a pathway that promotes chemoresistance,as well as a leading drug candidate to target this pathway.

关键词： pancreatic cancer TRA-1-60/TRA-1-81 chemoresistance UGT1A10 Cymarin

Plasma-Based Graphene Functionalization in Glow Discharge

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Graphene 2015年第1期4卷 1-6页

作者： X. Fang j. donahue A. Shashurin M. Keidar Department of Mechanical & Aerospace Engineering The George Washington University Washington DC USA

Glow discharge was utilized to add oxygen functional groups to the graphene platelets sample produced in chemical exfoliation synthesis. It was concluded based on Raman spectra that the graphene sample treated with the glow discharge preserves specific graphene features while no transformation to amorphous carbon is happening. SEM and EDS results indicated the increases of oxygen content in the graphene sample after the exposure to the glow discharge. Raman spectra also support the fact that the graphene platelets have been decorated with oxygen as the result of the glow discharge treatment.

关键词： Graphene Functionalization Plasma-Based

Peat record reflecting Holo-cene climatic change in the Zoige Plateau and AMS radiocarbon dating

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Chinese Science Bulletin 2002年第1期47卷 66-70页

作者： ZHOU Weij.an LU Xuefeng WU Zhengkun DENG Lin A.j.T.j.ll D.donahue W.Beck State Key Lab of Loess and Quaternary Geology Institute of Earth EnvironmentChinese Academy of SciencesXi’an 710054China NNSF-AMS Facility University of Arizona

Through the use of reliable AMS dating of high resolution (15-30 years) peat and the establishment of monsoon climate proxies sequence, we have been able to recognize several cold, dry events in the Tibetan Plateau during the Holocene. The more obvious ones occurred around 12800, 11300, 10200, 9580, 8900, 6400, 4400, 3700, 2800 and 1500 cal. aBP. These events correlate well with both ice rafting events recorded in high latitude North Atlantic Ocean sediment cores and cooling events in the low latitude SST. Spectral analysis indicates high frequency climate variation on centennial-millennial time scale during the Holocene. This further reflects Holocene climate instability and the existence of centennial-millenium scale rhythm in mid latitude areas as well.

关键词： Zoige Holocene high-resolution record peat AMS dating climate instability.

The missing base molecules in atmospheric acid-base nucleation

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National Science Review 2022年第10期9卷 196-208页

作者： Runlong Cai Ruj.ng Yin Chao Yan Dongsen Yang Chenj.an Deng Lubna Dada j.ha Kangasluoma j.nni Kontkanen Roope Halonen Yan Ma Xiuhui Zhang Pauli Paasonen Tuukka Pet?j. Veli-Matti Kerminen Yongchun Liu Federico Bianchi j.n Zheng Lin Wang j.ming Hao j.mes N.Smith Neil M. donahue Markku Kulmala Douglas R.Worsnop j.ngkun j.ang State Key j.int Laboratory of Environment Simulation and Pollution Control School of Environment Tsinghua University Institute for Atmospheric and Earth System Research/Physics Faculty of Science University of Helsinki Aerosol and Haze Laboratory Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical Technology Collaborative Innovation Center of Atmospheric Environment and Equipment Technology Nanjing University of Information Science and Technology Laboratory of Atmospheric Chemistry Paul Scherrer Institute Center for j.int Quantum Studies and Department of Physics School of Science Tianjin University Key Laboratory of Cluster Science Ministry of Education of China School of Chemistry and Chemical Engineering Beijing Institute of Technology Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP3) Department of Environmental Science and Engineering Fudan University Chemistry Department University of California Center for Atmospheric Particle Studies Carnegie Mellon University Department of Chemistry Carnegie Mellon University Aerodyne Research Inc.

Transformation of low-volatility gaseous precursors to new particles affects aerosol number concentration,cloud formation and hence the *** clustering of acid and base molecules is a maj.r mechanism driving fast nucleation and initial growth of new particles in the ***,the acid-base cluster composition,measured using state-of-the-art mass spectrometers,cannot explain the measured high formation rate of new *** we present strong evidence for the existence of base molecules such as amines in the smallest atmospheric sulfuric acid clusters prior to their detection by mass *** demonstrate that forming(H2SO4)1(amine)1is the rate-limiting step in atmospheric H2SO4-amine nucleation and the uptake of(H2SO4)1(amine)1is a maj.r pathway for the initial growth of *** proposed mechanism is very consistent with measured new particle formation in urban Beij.ng,in which dimethylamine is the key base for H2SO4nucleation while other bases such as ammonia may contribute to the growth of larger *** findings further underline the fact that strong amines,even at low concentrations and when undetected in the smallest clusters,can be crucial to particle formation in the planetary boundary layer.

关键词： new particle formation acid-base nucleation aerosol polluted urban environment

Overexpression of OGFr Downregulates Ovarian Cancer Cell Proliferation <i>In Vitro</i>and Inhibits Tumorigenesis

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j.urnal of Cancer Therapy 2011年第4期2卷 579-594页

作者： Renee N. donahue Patricia j. McLaughlin Ian S. Zagon 不详

The opioid growth factor (OGF) and its receptor (OGFr) regulate human ovarian cancer cell proliferation through a tonically active inhibitory axis. We investigated the effect of OGFr overexpression on ovarian tumorigenesis. Clonal cell lines of SKOV-3 human ovarian cancer were established to stably overexpress OGFr. shRNA constructs were evaluated for antitumor activity in vitro, as well as in vivo using mouse models of s.c. and i.p. tumor transplantation. The 5 clonal cell lines were characterized by increases in OGFr protein (62% to 245%) and binding capacity (51% - 154%), and decreases (36% - 185%) in cell number, relative to untransfected wild-type (WT) cells and empty vector (EV) transfected clones. Nude mice receiving s.c. inj.ction of 2 overexpressing OGFr cell lines (OGFr-3 and OGFr-22) had reduced tumor incidence, delayed tumor appearance (up to 12 days), and decreased tumor volume (up to 87%) relative to WT and EV controls. Mice inj.cted i.p. with these clonal lines displayed reduced formation of tumor nodules (up to 95%), and depressed tumor weights (up to 99%) compared to WT and EV groups. DNA synthesis, but not cell survival, was depressed in cells and s.c. tumors overexpressing OGFr in comparison to the WT and EV groups. Angiogenesis was reduced up to 86% in clonal tumors compared to WT and EV groups. This preclinical evidence demonstrates that OGFr expression is a molecular determinant of ovarian cancer progression, and has important relevance to understanding the pathogenesis and treatment of this deadly disease.

关键词： Cell Proliferation Opioids [Met5]-Enkephalin OGF OGFr Ovarian Cancer

The Opioid Growth Factor Inhibits Established Ovarian Cancer in Nude Mice and Can Be Combined with Taxol or Cisplatin to Enhance Growth Inhibition

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j.urnal of Cancer Therapy 2011年第2期2卷 110-124页

作者： Renee N. donahue Ian S. Zagon Patricia j. McLaughlin Department of Neural and Behavioral Sciences The Pennsylvania State University College of Medicine Hershey PA

Ovarian cancer is the 5th leading cause of cancer-related mortality in women. Seventy-five percent of ovarian cancer patients present in advanced stages, and receive cytoreductive surgery and adj.vant chemotherapy. However, within 2 years 65% of these patients relapse and thereafter only receive palliative care. Novel therapies based on the biology of these cancers are urgently needed. The opioid growth factor (OGF)-OGF receptor (OGFr) axis is an endogenous opioid system known to inhibit proliferation of human ovarian cancer cells in tissue culture, but does not affect cell survival. The present study determined whether OGF in combination with standard of care chemotherapy, provides an inhibitory effect on the growth of human ovarian cancer cells in vitro. In addition, this investigation assessed whether OGF biotherapy, alone or in combination with taxol or cisplatin, inhibits tumor growth in mice with xenografts of ovarian cancer. The combination of OGF (10–6 M) with taxol (10–9 M or 10–10 M) or cisplatin (0.01 ug/ml or 0.001 ug/ml) markedly reduced cell number and DNA synthesis in vitro to a greater extent than individual compounds. OGF, but not taxol or cisplatin, altered growth in an opioid receptor mediated and reversible manner. Female nu/nu mice inoculated subcutaneously with SKOV-3 cells, and treated with OGF (10 mg/kg) for 5 weeks commencing at the time tumors became measurable, had tumor volumes and weight that were reduced by up to 50% from animals receiving saline. The combination of OGF with taxol (3 mg/kg, weekly) or cisplatin (4 mg/kg, weekly for 2 weeks) for 37 days reduced tumor volumes and weight in contrast to mice receiving individual agents alone. Moreover, OGF treatment in mice receiving cisplatin provided protection against the weight loss associated with cisplatin alone. All treatments suppressed DNA synthesis and angiogenesis, whereas exposure to taxol or cisplatin, but not OGF, induced apoptosis. Additive inhibitory effects on DNA synthesis

关键词： Cell Proliferation [Met5]-Enkephalin Opioid Growth Factor OGFr Ovarian Cancer

南海全新世珊瑚礁AMS^(14)C,^(230)Th/^(234)U和ESR年龄的对比研究

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海洋科学 1993年第2期17卷 63-65页

作者：业渝光 D.j.donahue 地质矿产部海洋地质研究所青岛266071 美国亚利桑那大学

用^(14)C(AMS和稀释技术),铀系和ESR方法对南海珊瑚礁进行了测年对比研究,表明这些珊瑚礁形成于全新世早、中期。此外,对测年方法的一些有关问题也进行了讨论。

关键词：珊瑚礁 AMS^14C ESR 铀系年龄