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外源刺激剂强化芽孢杆菌高效修复Cr(Ⅵ)污染水体

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农业环境科学学报 2024年第5期43卷 1100-1113页

作者：张亚珍向媛羚张庆明康茜琳张恒星张伟韩颖西南科技大学生命科学与工程学院四川绵阳621010 西南科技大学应用技术学院四川绵阳621010 西南科技大学分析测试中心四川绵阳621010

目前,利用微生物修复六价铬[Cr(Ⅵ)]污染的土壤和水体已经在实验室及场地大量应用,但由于土著微生物的竞争及场地的寡营养条件,导致外源铬(Cr)高效还原微生物定殖困难。鉴于此,本研究从成本低廉且无二次污染的氮源型/碳源型/矿物型/离... 详细信息

目前,利用微生物修复六价铬[Cr(Ⅵ)]污染的土壤和水体已经在实验室及场地大量应用,但由于土著微生物的竞争及场地的寡营养条件,导致外源铬(Cr)高效还原微生物定殖困难。鉴于此,本研究从成本低廉且无二次污染的氮源型/碳源型/矿物型/离子型的微生物刺激剂筛选出发,探讨了不同刺激剂添加方式(非包埋型和包埋型)对抗铬芽孢杆菌(Bacillus sp.)高效还原Cr(Ⅵ)的影响,并采用扫描电子显微镜-能谱(SEM-EDS)对芽孢杆菌表面进行表征。结果表明,甘蔗粉(碳源型)、豆粕粉(氮源型)、钙离子[Ca(Ⅱ),离子型]、蒙脱石(矿物型)这四种刺激剂的添加显著增加了芽孢杆菌对Cr(Ⅵ)的还原效率,进一步利用单因素多水平试验确定了它们的最佳投加量分别为10 g∙L^(-1)、10 g∙L^(-1)、10 mmol∙L^(-1)、8 g∙L^(-1),在第8天时与裸菌相比,分别添加这4种刺激剂的芽孢杆菌对Cr(Ⅵ)的还原率分别提高了17.48、15.56、11.70、18.84个百分点。在单因素试验的基础上,挑选2个效果最佳的刺激剂甘蔗粉和蒙脱石,利用响应曲面法建立了混合型刺激剂(甘蔗粉+蒙脱石)与菌的最佳投加量,结果显示甘蔗粉投加量为8 g∙L^(-1)、蒙脱石投加量为6 g∙L^(-1)、菌剂投加量为0.3%(V/V)时,培养8 d后,与裸菌相比,添加混合刺激剂的芽孢杆菌对Cr(Ⅵ)还原率增加了32.53个百分点,显著高于单一非混合刺激组。考虑到刺激剂直接加入环境亦会被土著微生物利用,导致对外源铬还原微生物刺激作用的长效性差,本研究还探究了包埋型缓释刺激剂对芽孢杆菌还原Cr(Ⅵ)的长效刺激作用,结果发现,在包埋型刺激剂的辅助下,芽孢杆菌对Cr(Ⅵ)的还原能力随着时间延长而不断增加,其Cr(Ⅵ)还原率分别从86.08%(第2天)增加到97.09%(第28天),添加了非包埋型混合刺激剂的菌从86.92%(第2天)下降到75.93%(第28天)。SEM观察修复28 d的菌,发现未加任何刺激剂的裸菌出现严重皱缩,部分断裂,形态结构不完整;添加非包埋混合刺激剂的菌出现轻微皱缩,点状断裂,形态结构较为完整;而添加包埋型刺激剂的菌光滑,形态结构完整,表面有绒毛状产物,EDS显示绒毛状产物O和Cr含量相对于其他元素较多,包埋型刺激剂提升了菌细胞表面参与的铬固定,降低了铬对细菌细胞的毒性。研究表明,混合刺激剂还原效果优于单一非混合刺激组,确定最佳投加量分别为甘蔗粉(8 g∙L^(-1))、蒙脱石(6 g∙L^(-1))和菌剂(0.3%)。利用非包埋型刺激剂有利于短期内快速提升菌对Cr(Ⅵ)还原,而包埋型刺激剂使菌还原Cr(Ⅵ)具有长效性并有效地降低了Cr(Ⅵ)毒性。

关键词： Cr(Ⅵ)污染非包埋型刺激剂包埋型刺激剂芽孢杆菌 Cr(Ⅵ)还原效率

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高硅锌精矿焙烧过程中硅的控制及热平衡计算

有色金属工程 2024年第1期14卷 61-66页

作者：张恒星陈杭陈芬陈期生应宗波吴星琳王俊娥福建紫金贵金属材料有限公司福建上杭364200 紫金铜业有限公司福建上杭364200 厦门紫金矿冶技术有限公司福建厦门361115

针对高硅锌精矿焙烧过程中焙砂可溶硅高、沸腾炉易结块、浸出固液分离困难等问题,以现场生产焙烧工艺参数为基础,研究了低温和高温焙烧对焙砂中可溶硅含量的影响,并基于MatCal软件对沸腾炉焙烧工艺进行热平衡计算。结果表明:在焙烧条件... 详细信息

针对高硅锌精矿焙烧过程中焙砂可溶硅高、沸腾炉易结块、浸出固液分离困难等问题,以现场生产焙烧工艺参数为基础,研究了低温和高温焙烧对焙砂中可溶硅含量的影响,并基于MatCal软件对沸腾炉焙烧工艺进行热平衡计算。结果表明:在焙烧条件基本相同的情况下,随着硫化锌精矿焙烧温度的增加,焙砂中的可溶硅也增加。当焙砂中可溶硅高于3.18%会出现浓密机上清液跑混、低浸浓密底流矿浆过滤困难、净液中除杂钴偏高等问题。经MatCal模拟计算后,理论消耗空气50361.328 m 3/h,低温焙烧的平均风量47102.8 m 3/h,高温焙烧平均风量48005.7 m 3/h,实际的焙烧中平均风量偏低,需要增加沸腾炉的风料比。

关键词：硫化锌精矿沸腾炉焙烧热平衡

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Performance of MODIS aerosol products at various timescales and in different pollution conditions over eastern Asia

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Science China(Technological Sciences) 2021年第4期64卷 774-784页

作者： MAO QianJun HUANG ChunLin zhang hengxing CHEN QiXiang YUAN Yuan School of Urban Construction Wuhan University of Science and TechnologyWuhan 430065China Key Laboratory of Aerospace Thermophysics Ministry of Industry and Information TechnologyHarbin Institute of TechnologyHarbin 150001China

High-quality aerosol optical depth(AOD)data derived from MODIS is widely used in studying spatiotemporal trends of fine particulate matter(PM2.5)concentrations in eastern ***,the differences of MODIS-AOD(3/10 km DT;10 km DB)under four pollution situations(No-Po;Sl-Po;Mo-Po;Se-Po)are rarely *** this study,the MODIS-AOD and AODDifference spatial distributions from 2008 to 2017 are analyzed through annual/seasonal mean AOD maps generated at 0.1°×0.1°*** MODIS-AOD performances are validated using AERONET AOD data for various pollution situations and aerosol *** validations indicate that the 10-km DB algorithm provides the best performance,followed by 3-km DTand 10 km *** DB algorithm can provide spatially continuous AOD data for all seasons,whereas the DT algorithm often fails to yield valid data during *** validations under different pollution conditions illustrate that severe pollution significantly affects the validity of data obtained by the DB ***,the accuracy of DT-derived AOD data is robust against *** the same pollution conditions,the correlation coefficient of the DB algorithm is smaller than that of the DT *** quantity of valid data in the DB product is higher than those in DT products for all pollution conditions,especially under Se-Po.

关键词： aerosol optical depth MODIS DT algorithm DB algorithm pollution conditions

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Detection method of absence seizures based on Resnet and bidirectional GRU

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Acta Epileptologica 2023年第1期6卷 34-42页

作者： Lijun Li hengxing zhang Xiaomei Liu Jie Li Lei Li Dan Liu Jieqing Min Ping Zhu Huan Xia Shangkun Wang Li Wang Kunming children‘s hospital Kunming 650000China Zhengzhou Zoneyet Technology Corp. LtdZhengzhou 450000China

Background Epilepsy is a common chronic neurological *** repeated seizure attacks have a great negative impact on patients’physical and mental *** diagnosis of epilepsy mainly depends on electroencephalogram(EEG)signals detection and *** are two main EEG signals detection methods for *** is the detection based on abnormal waveform,the other is the analysis of EEG signals based on the traditional machine *** feature extraction method of the traditional machine learning is difcult to capture the high-dimension information between adjacent *** In this paper,redundant information was removed from the data by Gaussian fltering,downsampling,and short-time Fourier *** Neural Networks(CNN)was used to extract the high-dimensional features of the preprocessed data,and then Gate Recurrent Unit(GRU)was used to combine the sequence information before and after,to fully integrate the adjacent information EEG signals and improve the accuracy of the model *** Four models were designed and *** experimental results showed that the prediction model based on deep residual network and bidirectional GRU had the best efect,and the test accuracy of the absence epilepsy test set reached 92%.Conclusions The prediction time of the network is only 10 sec when predicting four-hour EEG *** can be efectively used in EEG software to provide reference for doctors in EEG analysis and save doctors’time,which has great practical value.

关键词： Resnet Epilepsy GRU Electroencephalogram

Na_(3)P interphase reduces Na nucleation energy enabling stable anode-less sodium metal batteries

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Journal of Energy Chemistry 2024年第9期96卷 448-455页

作者： Haizhao Yang Haifeng Lv En Zhou Xiaohao Ji Chunnian Chen Haolei Yu Zhaowei Sun Dawei zhang Hongchang Jin Xianghua Kong hengxing Ji Department of Chemistrynd Chemical Engineering Hefei University of TechnologyHefei 230009AnhuiChina Hefei National Research Center for Physical Sciencest the Microscale School of Chemistrynd Materials ScienceUniversity of Sciencend Technology of ChinaHefei 230026AnhuiChina

Sodium metal batteries(SMBs)are rising as viable alternatives to lithium-ion systems due to their superior energy density and sodium's relative ***,SMBs face significant impediments,particularly the exceedingly high negative-to-positive capacity ratios(N/P ratios)which severely encumber energy density and hinder their practical ***,a novel nucleophilic Na_(3)P interphase on aluminum foil has been designed to significantly lower the nucleation energy barrier for sodium atom deposition,resulting in a remarkable reduction of nucleation overpotential and efficient mitigation of dendritic growth at high sodium deposition of 5 mA h cm^(−2).The interphase promotes stable cycling in anode-less SMB configurations with a low N/P ratio of 1.4 and high cathode mass loading of 11.5 mg cm^(−2),and demonstrates a substantial increase in high capacity retention of 92.4%after 500 cycles even under 1 C rate *** innovation signifies a promising leap forward in the development of high-energy-density,anode-less SMBs,offering a potential solution to the longstanding issues of cycle stability and energy efficiency.

关键词： Anode-less Phosphorus anode Nucleation buffer layer Sodium metal Dendrite-free

Climate change drives flooding risk increases in the Yellow River Basin

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Geography and Sustainability 2024年第2期5卷 193-199页

作者： hengxing Lan Zheng Zhao Langping Li Junhua Li Bojie Fu Naiman Tian Ruixun Lai Sha Zhou Yanbo Zhu Fanyu zhang Jianbing Peng John J.Clague State Key Laboratory of Resources and Environmental Information System Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China School of Geological Engineering and Geomatics Chang’an UniversityXi’an 710064China University of Chinese Academy of Sciences Beijing 10049China Yellow River Institute of Hydraulic Research Yellow River Conservancy CommissionZhengzhou 450003China State Key Laboratory of Urban and Regional Ecology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing 100085China State Key Laboratory of Earth Surface Processes and Resources Ecology Beijing Normal UniversityBeijing 100875China Key Laboratory of Mechanics on Disaster and Environment in Western China The Ministry of Education of ChinaSchool of Civil Engineering and MechanicsLanzhou UniversityLanzhou 730000China Department of Earth Sciences Simon Fraser UniversityBurnabyCanada

The Yellow River Basin(YRB)has experienced severe floods and continuous riverbed elevation throughout *** climate change has been suggested to be driving a worldwide increase in flooding ***,owing to insufficient evidence,the quantitative correlation between flooding and climate change remains *** present a long time series of maximum flood discharge in the YRB dating back to 1843 compiled from historical documents and instrument *** in yearly maximum flood discharge show distinct periods:a dramatic decreasing period from 1843 to 1950,and an oscillating gentle decreasing from 1950 to 2021,with the latter period also showing increasing more extreme floods.A Mann-Kendall test analysis suggests that the latter period can be further split into two distinct sub-periods:an oscillating gentle decreasing period from 1950 to 2000,and a clear recent increasing period from 2000 to *** further predict that climate change will cause an ongoing remarkable increase in future flooding risk and an∼44.4 billion US dollars loss of floods in the YRB in 2100.

关键词： Flooding risk Risk management Climate change Flood discharge Extreme precipitation

Transcription factor EHF interacting with coactivator AJUBA aggravates malignancy and acts as a therapeutic target for gastroesophageal adenocarcinoma

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Acta Pharmaceutica Sinica B 2024年第5期14卷 2119-2136页

作者： Li Peng Yanyi Jiang hengxing Chen Yongqiang Wang Qiusheng Lan Shuiqin Chen Zhanwang Huang Jingyuan zhang Duanqing Tian Yuntan Qiu Diankui Cai Jiangyun Peng Daning Lu Xiaoqing Yuan Xianzhu Yang Dong Yin Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Guangdong-Hong Kong Joint Laboratory for RNA MedicineSun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Medical Research Center Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Hefei Institutes of Physical Science Chinese Academy of SciencesHefei 230031China Department of Gastrointestinal Surgery Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Department of Hepatobiliary Surgery Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China School of Biomedical Sciences and Engineering South China University of TechnologyGuangzhou International CampusGuangzhou 511442China

Transcriptional dysregulation of genes is a hallmark of tumors and can serve as targets for cancer drug ***,it is extremely challenging to develop small-molecule inhibitors to target abnormally expressed transcription factors(TFs)except for the nuclear receptor family of *** is known about the interaction between TFs and transcription cofactors in gastroesophageal adenocarcinoma(GEA)or the therapeutic effects of targeting TF and transcription cofactor *** this study,we found that ETS homologous factor(EHF)expression is promoted by a core transcriptional regulatory circuitry(CRC),specifically ELF3-KLF5-GATA6,and interference with its expression suppressed the malignant biological behavior of GEA ***,we identified Ajuba LIM protein(AJUBA)as a new coactivator of EHF that cooperatively orchestrates transcriptional network activity in ***,we identified KRAS signaling as a common pathway downstream of EHF and ***,dual targeting of EHF and AJUBA by lipid nanoparticles cooperatively attenuated the malignant biological behaviors of GEA in vitro and in *** conclusion,EHF is upregulated by the CRC and promotes GEA malignancy by interacting with AJUBA through the KRAS *** of both EHF and its coactivator AJUBA through lipid nanoparticles is a novel potential therapeutic strategy.

关键词： EHF AJUBA KRAS pathway Enhancer Core transcriptional regulatory circuitry Gastroesophageal adenocarcinoma Gastric adenocarcinoma Esophageal adenocarcinoma Transcription factor Coactivator Lipid nanoparticles

V-BILIPSCHITZ DETERMINACY OF WEIGHTED HOMOGENEOUS ANALYTIC FUNCTION-GERMS ON WEIGHTED HOMOGENEOUS REAL ANALYTIC VARIETIES

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Acta Mathematica Scientia 2010年第4期30卷 1249-1256页

作者：刘恒兴张敦穆 School of Mathematics and Statistics Wuhan University

In this article, we provide estimates for the degree of V bilipschitz determinacy of weighted homogeneous function germs defined on weighted homogeneous analytic variety V satisfying a convenient Lojasiewicz *** result gives an explicit order such that the geometrical structure of a weighted homogeneous polynomial function germs is preserved after higher order perturbations.

关键词： V bilipschitz determinacy weighted homogeneous polynomial function germs controlled vector field weighted homogeneous control functions

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HiFAST:An HIdata calibration and imaging pipeline for FAST

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Science China(Physics,Mechanics & Astronomy) 2024年第5期67卷 182-199页

作者： Yingjie Jing Jie Wang Chen Xu Ziming Liu Qingze Chen Tiantian Liang Jinlong Xu Yixian Cao Jing Wang Huijie Hu Chuan-Peng zhang Qi Guo Liang Gao Mei Ai Hengqian Gan Xuyang Gao Jinlin Han Ligang Hou Zhipeng Hou Peng Jiang Xu Kong Fujia Li Zerui Liu Li Shao hengxing Pan Jun Pan Lei Qian Jinghai Sun Ningyu Tang Qingliang Yang Bo zhang Zhiyu zhang Ming Zhu National Astronomical Observatories Chinese Academy of SciencesBeijing 100012China Institute for Frontiers in Astronomy and Astrophysics Beijing Normal UniversityBeijing 02206China School of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 100049China Guizhou Radio Astronomical Observatory Guizhou UniversityGuiyang 55000China Max Planck Institute for Extraterrestrial Physics Garching 85748Germany Kavli Institute for Astronomy and Astrophysics Peking UniversityBeijing 100871China Institute of Computational Cosmology Department of PhysicsUniversity of DurhamDurham DHI 3LEUK CAS Key Laboratory of FAST National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China Deep Space Exploration Laboratory/Department of Astronomy University of Science and Technology of ChinaHefei 230026China School of Astronomy and Space Science University of Science and Technology of ChinaHefei 230026China Astrophysics Department of PhysicsUniversity of OxfordOxford OX/3RHUK College of Earth Sciences Guilin University of TechnologyGuilin 541004China Department of Physics Anhui Normal UniversityWuhu 241002China School of Astronomy and Space Science Nanjing UniversityNanjing 210023China Key Laboratory of Modern Astronomy and Astrophysics Nanjing UniversityNanjing 20023China

The Five-hundred-meter Aperture Spherical radio Telescope(FAST)has the largest aperture and a 19-beam L-band receiver,making it powerful for investigating the neutral hydrogen atomic gas(HI)in the *** present HiFAST(https://***),a dedicated,modular,and self-contained calibration and imaging pipeline for processing the H?data of *** pipeline consists of frequency-dependent noise diode calibration,baseline fitting,standing wave removal using an FFT-based method,flux density calibration,stray radiation correction,and gridding to produce data *** modules can be combined as needed to process the data from most FAST observation modes:tracking,drift scanning,On-The-Fly mapping,and most of their *** HiFAST,the root-mean-square(RMS)noises of the calibrated spectra from all 19 beams were only slightly(~5%)higher than the theoretical *** results for the extended source M33 and the point sources are consistent with the results from *** moment maps(0,1 and 2)of M33 agree well with the results from the Arecibo Galaxy Environment Survey(AGES)with a fractional difference of less than 10%.For a common sample of 221 sources with signal-to-noise ratio S/N>10 from the Arecibo Legacy Fast ALFA(ALFALFA)survey,the mean value of fractional difference in the integrated flux density,Sint,between the two datasets is approximately 0.005%,with a dispersion of 15.4%.Further checks on the integrated flux density of 23 sources with seven observations indicate that the variance in the flux density of the source with luminous objects(Sint>2.5 Jy km s-1)is less than 5%.Our tests suggest that the FAST telescope,with the efficient,precise,and user-friendly pipeline HiFAST,will yield numerous significant scientific findings in the investigation of the H?in the universe.

关键词： fractional luminous correction