检索结果-南通市图书馆

The LXRB-SREBP1 network regulates lipogenic homeostasis by controlling the synthesis of polyunsaturated fatty acids in goat mammary epithelial cells

引用

Journal of Animal Science and Biotechnology 2023年第2期14卷 614-626页

作者： Wenying Zhang Changhui Zhang Jun Luo Huifen Xu Jianxin Liu Juan JLoor Hengbo shi Institute of Dairy Science College of Animal SciencesZhejiang UniversityHangzhou 310058China College of Animal Science and Technology Northwest A&F UniversityYangling 712100China College of Animal Science and Technology Henan Agricultural UniversityZhengzhou 450046China Key Laboratory of Molecular Animal Nutrition(Zhejiang University) Ministry of EducationHangzhou310058China Mammalian Nutrition Physiology Genomics Department of Animal Sciences and Division of Nutritional SciencesUniversity of IllinoisUrbanaIL 61801USA.

Background:In rodents,research has revealed a role of liver X receptors(LXR) in controlling lipid homeostasis and regulating the synthesis of polyunsaturated fatty acids(PUFA).Recent data suggest that LXRB is the predominant LXR subtype in ruminant mammary cells,but its role in lipid metabolism is unknown.It was hypothesized that LXRB plays a role in lipid homeostasis via altering the synthesis of PUFA in the ruminant mammary gland.We used overexpression and knockdown of LXRB in goat primary mammary epithelial cells(GMEC) to evaluate abundance of lipogenic enzymes,fatty acid profiles,content of lipid stores and activity of the stearoyl-Co A desaturase(SCD1) promoter.Results:Overexpression of LXRB markedly upregulated the protein abundance of LXRB while incubation with si RNA targeting LXRB markedly decreased abundance of LXRB protein.Overexpression of LXRB plus T0901317(T09,a ligand for LXR) dramatically upregulated SCD1 and elongation of very long chain fatty acid-like fatty acid elongases 5–7(ELOVL 5–7),which are related to PUFA synthesis.Compared with the control,cells overexpressing LXRB and stimulated with T09 had greater concentrations of C16:0,16:1,18:1n7,18:1n9 and C18:2 as well as desaturation and elongation indices of C16:0.Furthermore,LXRB-overexpressing cells incubated with T09 had greater levels of triacylglycerol and cholesterol.Knockdown of LXRB in cells incubated with T09 led to downregulation of genes encoding elongases and desaturases.Knockdown of LXRB attenuated the increase in triacylglycerol and cholesterol that was induced by T09.In cells treated with dimethylsulfoxide,knockdown of LXRB increased the concentration of C16:0 at the expense of C18:0,while a significant decrease in C18:2 was observed in cells incubated with both si LXRB and T09.The abundance of sterol regulatory element binding transcription factor 1 precursor(p SREBP1) and its mature fragment(n SREBP1) was upregulated by T09,but not LXRB overexpression.In the cells cultured with T09,knockdown of LXRB downregulated the abundance

关键词： Elongase Lipid homeostasis Liver X receptor Mammary gland Polyunsaturated fatty acids

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

An operation optimization method of a fully mechanized coal mining face based on semi-physical virtual simulation

引用

International Journal of Coal Science & Technology 2020年第1期7卷 147-163页

作者： Hengbo shi Jiacheng Xie Xuewen Wang Juanli Li Xing Ge College of Mechanical and Vehicle Engineering Key Laboratory of Fully Mechanized Coal Mining EquipmentTaiyuan University of TechnologyTaiyuan 030024ShanxiChina Taiyuan Mining Machinery Group Co. Ltd.Postdoctoral Science Research WorkstationTaiyuan 030032ShanxiChina

A mathematical hydraulic support self-tracking model for three-machine cooperative mining is proposed to address low efficiency and difficulties in strategy evaluation of a fully mechanized coal face.The proposed model uses the coordinates and traction speed of the shearer to calculate the frequency of the circular hydraulic support and realize the coordinated operation of the three-machine mining technology.A unity3d hardware-in-the-loop simulation experimental hearer and hydraulic support platform was used to validate the model of autonomous follow-up.The results indicate that collaborative control of coal mining allowed for an efficiency 3.76%higher than under automatic operation mode and 46.03%higher than under manual control;thus,The mathematical model provided an improved production efficiency of the fully mechanized mining face.The mathematical model also provides a more intelligent and reliable security support,and improves the intelligent level of hydraulic support follow-up control.

关键词： Semi-physical simulation Virtual reality Unity3d Human-computer interaction Shearer traction speed

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

AMPK-ChREBP axis mediates de novo milk fatty acid synthesis promoted by glucose in the mammary gland of lactating goats

引用

Animal Nutrition 2022年第3期10卷 234-242页

作者： Hengbo shi Nannan Jiang Ling Wei Jie Cai Wenying Zhang Qianming Jiang Juan JLoor Jianxin Liu Institute of Dairy Science College of Animal SciencesZhejiang UniversityHangzhou310058China College of Life Sciences and Medicine Zhejiang Sci-Tech UniversityHangzhou310018China Mammalian Nutrition Physiology Genomics Department of Animal Sciences and Division of Nutritional SciencesUniversity of IllinoisUrbanaIL61801USA

To investigate the role of glucose in regulating milk fatty acid synthesis,6 lactating Guanzhong dairy goats were infused with 0,60,or 100 g/d glucose via the external pubic artery in a 33 repeated Latin square experiment.A concomitant in vitro experiment was conducted to investigate possible mechanisms whereby glucose regulates milk fatty acid synthesis.RNA sequencing was used for cellular transcriptome analysis.Drugs,MK-2206,rapamycin,and dorsomorphin were used to block cellular mammalian AMP-activated protein kinase(AMPK),AKT serine/threonine kinase 1,and mechanistic target of rapamycin kinase signaling pathways,respectively.Carbohydrate response element binding protein(ChREBP)was knockdown and overexpressed to investigate its role in regulating milk fatty acid synthesis in mammary epithelial cells.Glucose infusion linearly elevated the concentration of C8:0(P=0.039)and C10:0(P=0.041)in milk fat while it linearly decreased(P=0.049)that of C16:0.This result was in agreement with the upregulation of genes related to de novo synthesis of fatty acids and lipid droplet formation,including adipose differentiation-related protein,butyrophilin subfamily 1 member A1,fatty acid synthase(FASN)and ChREBP.Their expression increased(P<0.05)linearly in the lactating goat mammary gland.In vitro,glucose linearly stimulated the expression of genes related to de novo synthesis of fatty acids and cellular triacylglycerol in cultured mammary epithelial cells.RNA sequencing and inhibition studies revealed that glucose induced transcriptomic changes increasing lipogenic pathways,with AMPK responding to glucose by controlling ChREBP and FASN.Knockdown and overexpression of ChREBP highlighted its essential role in lipogenesis.The knockdown and overexpression of ChREBP protein also revealed an essential role in regulating the de novo synthesis of fatty acids.Collectively,our data highlight that glucose supplementation promotes de novo fatty acid synthesis via the AMPK-ChREBP axis,hence increasing milk fat yield in the goat mammary gla

关键词： Milk fat Glucose infusion Mammary gland Carbohydrate response element binding protein De novo synthesis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

吴大羽对抽象表现绘画的承续与背离

引用

美术 2020年第10期634卷 136-137页

作者：石珩伯宝鸡文理学院美术学院

抽象表现绘画是西方艺术史在进入现代主义叙事阶段后,具有标志性绘画风潮之一。而言及中国20世纪延续至今的抽象表现绘画,它与西方抽象表现绘画的演进脉络关系密切,并且在形式、内容、创作理念诸方面都受其影响,但同时也应看到西方抽象... 详细信息

抽象表现绘画是西方艺术史在进入现代主义叙事阶段后,具有标志性绘画风潮之一。而言及中国20世纪延续至今的抽象表现绘画,它与西方抽象表现绘画的演进脉络关系密切,并且在形式、内容、创作理念诸方面都受其影响,但同时也应看到西方抽象表现绘画在被中国艺术家接受并将其作为一种创作形态后,通过相当一部分中国艺术家不懈的努力,在西方抽象表现绘画与中国传统绘画之间建立了某种值得深思的关联。

关键词：西方艺术史吴大羽抽象表现绘画中国传统绘画创作形态演进脉络创作理念标志性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ammonium Metavanadate Fabricated by Selective Precipitation of Impurity Chemicals on Inorganic Flocculants

引用

Journal of Renewable Materials 2023年第4期11卷 1951-1961页

作者： Bo shi Dandan Zhu Pengxiang Lei Ximin Li hengbo Xiao Lihua Qian Shandong Aluminum Industry Corporation Limited ZiboChina Petroleam&Natural Gas Transport Corporation of China WuhanChina School of Chemistry and Chemical Engineering Hubei University of TechnologyWuhanChina School of Physics Huazhong University of Science and TechnologyWuhanChina

High purity ammonium metavanadate(NH_(4)VO_(3))is the most vital chemical to produce V2O5,VO2,VN alloy,VFe alloy and VOSO4,which have some prospective applications for high strength steel,smart window,infrared detector and imaging,large scale energy storage system.NH_(4)VO_(3)is usually produced by spontaneous crystallization from the aqueous solution due to its sharp dependence of solubility on the temperature.However,hazardous chemicals in industrial effluent,include phosphorate,silicate and arsenate,causing severe damage to the environment.In this work,these impurities are selectively precipitated onto inorganic flocculants,while the vanadate dissolved in an aqueous solution keeps almost undisturbed.Therefore,high purity NH_(4)VO_(3)is produced by the crystallization from the purified solution.By screening various flocculants and precipitating parameters,polyaluminum sulfate with an optimal amount of 50 g/L,is demonstrated to selectively remove phosphorate,silicate and arsenate,corresponding to the removing efficiency of 93.39%,97.11%and 88.31%,respectively.NH_(4)VO_(3)from the purified solution holds a purity of 99.21%,in comparison with 98.33%in the product from the crude solution.This purifying technology cannot only produce NH_(4)VO_(3)with high added value,but also reduce the environmental pollution of waste liquid.

关键词： Ammonium metavanadate flocculating agent phosphate arsenate cooling precipitation vanadium slag bayer alumina liquor

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A fragment activity assay reveals the key residues of TBC1D15 GTPase-activating protein(GAP) in Chiloscyllium plagiosum

A fragment activity assay reveals the key residues of TBC1D1...

引用

中国生物化学与分子生物学会第十二届全国会员代表大会暨2...

作者： Yangyang Jin Yanna Chen Jianqing Chen Dan Wang Hengbo shi Hui Fei Zhengbing Lv Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine College of Life ScienceZhejiang Sci-Tech University

GTPase-activating proteins(GAPs) with a TBC(Tre-2/Bub2/Cdc16) domain architecture serve as negative regulators of Rab GTPases.Here,we analyzed the Rab-GAP specificity of TBC1 D15 in the evolution and influence of key amino acid residue mutations on Rab-GAP activity.Sequence alignment showed that five arginine residues of the TBC1 D15-GAP domain are conserved among the species Sus/Mus/Homo but have been replaced by glycine or lysine in Shark.A fragment activity assay was conducted by altering the five residues of Shark TBC1 D15-GAP to arginine,and the corresponding arginine in TBC1 D15 GAP domains from Sus and Homo species were mutated to resemble Shark TBC1 D15-GAP.Our data revealed that the residues of G28,K45,K119,K122 and K221 in the Shark TBC1 D15-GAP domain had a key role in determining the specificity for Rab7 and Rab11.Mutation of the five residues significantly altered the Shark TBC1 D15-GAP activity.These results revealed that the substrate specificity of TBC1 D15 had different mechanisms across the evolution of species from lower-cartilaginous fish to higher mammals.Collectively,the data support a different mechanism of Shark TBC1 D15-GAP in substrate selection,which imitates the function of Shark TBC1 D15-GAP in marine drug development.

关键词： TBC1D15 Rab-GAP activity evolution substrate specificity

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

汽车制动盘温度场瞬态分析方法的研究

引用

汽车技术 2016年第6期 1-4,10页

作者：朱晴陈群史亨波中国第一汽车股份有限公司技术中心长春130011

针对汽车制动盘在整车热容量工况下温度变化的问题,建立运用Starccm+软件与Rad Therm软件耦合的制动盘温度场瞬态仿真计算分析方法,其计算结果与供应商计算结果对比误差在合理范围内,说明该计算方法合理可行。运用该方法对4种不同结构... 详细信息

针对汽车制动盘在整车热容量工况下温度变化的问题,建立运用Starccm+软件与Rad Therm软件耦合的制动盘温度场瞬态仿真计算分析方法,其计算结果与供应商计算结果对比误差在合理范围内,说明该计算方法合理可行。运用该方法对4种不同结构制动盘的温度场进行计算对比分析,得出方案2结构最优,该计算方法为制动盘的设计与选型提供依据。

关键词：制动盘温度场瞬态分析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

含水乙醇汽油简化化学反应动力学模型

引用

同济大学学报（自然科学版） 2018年第11期46卷 1528-1535页

作者：石秀勇康杨罗亨波倪计民同济大学汽车学院上海201804

引入"半解耦"方法,以H2/CO/C1小分子机理为"内核",耦合乙醇氧化骨架机理和汽油表征燃料的甲苯参比燃料(TRF)机理,构建乙醇汽油混合燃料的简化化学反应动力学模型.利用CHEMKIN软件进行数值模拟,通过相关实验对该模型进行验证.进一步分析... 详细信息

引入"半解耦"方法,以H2/CO/C1小分子机理为"内核",耦合乙醇氧化骨架机理和汽油表征燃料的甲苯参比燃料(TRF)机理,构建乙醇汽油混合燃料的简化化学反应动力学模型.利用CHEMKIN软件进行数值模拟,通过相关实验对该模型进行验证.进一步分析水的影响,构建了含水乙醇汽油简化化学反应动力学模型.与乙醇汽油着火特性实验数据进行对比,验证了该简化机理的合理性.

关键词：汽油含水乙醇化学反应机理简化模型

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

铝/钢固态焊接合界面金属间化合物生长机制

引用

焊接学报 2019年第6期40卷 58-63,I0003,I0004页

作者：申中宝邱然锋石红信马恒波河南科技大学洛阳471023 有色金属共性技术河南省协同创新中心洛阳471003

在保持固态条件下,分别变化加热温度、时间对铝/Q235钢爆炸焊接头进行加热处理.分析了接合界面区反应层形貌等微观特征,探讨了加热温度、加热时间对反应层厚度的影响,研究了接合界面金属间化合物的生长行为.界面反应物是由靠近铝合金侧... 详细信息

在保持固态条件下,分别变化加热温度、时间对铝/Q235钢爆炸焊接头进行加热处理.分析了接合界面区反应层形貌等微观特征,探讨了加热温度、加热时间对反应层厚度的影响,研究了接合界面金属间化合物的生长行为.界面反应物是由靠近铝合金侧的反应物为Fe4Al13和靠近钢侧反应物为Fe2Al5构成.金属间化合物层随着加热时间的延长而变厚.结果表明,金属间化合物的生长满足抛物线法则,其生长激活能为33.26kJ/mol.

关键词：纯铝低碳钢生长机制

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

直喷汽油机颗粒物生成及排放特性研究进展

引用

同济大学学报（自然科学版） 2017年第S1期45卷 144-151页

作者：石秀勇康杨倪计民罗亨波同济大学汽车学院上海201804

通过对缸内直喷汽油机颗粒物生成机理、排放特性以及影响因素的研究进行综述,了解到直喷汽油机碳烟的生成主要源于缸内壁面油膜的池火燃烧和局部混合气过浓的影响,多环芳香烃是碳烟生成的主要前驱物;直喷汽油机颗粒物排放要高于进气道... 详细信息

通过对缸内直喷汽油机颗粒物生成机理、排放特性以及影响因素的研究进行综述,了解到直喷汽油机碳烟的生成主要源于缸内壁面油膜的池火燃烧和局部混合气过浓的影响,多环芳香烃是碳烟生成的主要前驱物;直喷汽油机颗粒物排放要高于进气道喷射汽油机以及装有颗粒捕集器的柴油机,并且与发动机运行及控制参数、混合气组织方式、燃油理化特性等有较大关系,通过优化燃烧组织措施,采用含氧燃料等方法能有效降低颗粒物排放.

关键词：缸内直喷汽油机碳烟颗粒物排放特性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：