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Easily-handled method to isolate mesenchymal stem cells from coagulated human bone marrow samples

World Journal of Stem Cells 2015年第8期7卷 1137-1144页

作者： heng-xiang wang Zhi-Yong Li Zhi-Kun Guo Zi-Kuan Guo Department of Hematology General Hospital of Air Force Key and Open Lab for Tissue Regeneration Xinxiang Medical University Department of Experimental Hematology Beijing Radiation Medicine

AIM:To establish an easily-handled method to isolate mesenchymal stem cells(MSCs) from coagulated human bone marrow samples. METHODS: Thrombin was added to aliquots of seven heparinized human bone marrow samples to mimic marrow coagulation. The clots were untreated,treated with urokinase or mechanically cut into pieces before culture for MSCs. The un-coagulated samples and the clots were also stored at 4 ℃ for 8 or 16 h before the treatment. The numbers of colony-forming unit-fibroblast(CFU-F) in the different samples were determined. The adherent cells from different groups were passaged and their surface profile was analyzed with flow cytometry. Their capacities of in vitro osteogenesis and adipogenesis were observed after the cells were exposed to specific inductive ***: The average CFU-F number of urokinasetreated samples(16.85 ± 11.77/106) was comparable to that of un-coagulated control samples(20.22 ± 10.65/106,P = 0.293),which was significantly higher than those of mechanically-cut clots(6.5 ± 5.32/106,P < 0.01) and untreated clots(1.95 ± 1.86/106,P < 0.01). The CFU-F numbers decreased after samples were stored,but those of control and urokinase-treated clots remained higher than the other two groups. Consistently,the numbers of the attached cells at passage 0 were higher in control and urokinase-treated clots than those of mechanically-cut clots and untreated *** attached cells were fibroblast-like in morphology and homogenously positive for CD44,CD73 and CD90,and negative for CD31 and CD45. Also,they could be induced to differentiate into osteoblasts and adipocytes in vitro. CONCLUSION: Urokinase pretreatment is an optimal strategy to isolate MSCs from human bone marrow samples that are poorly aspirated and clotted.

关键词： Coagulated marrow sample Mesenchymal stem cells Is

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Impact of prophylactic/preemptive donor lymphocyte infusion and intensified conditioning for relapsed/refractory leukemia:a real-world study

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Science China(Life Sciences) 2020年第10期63卷 1552-1564页

作者： Yu wang Qi-Fa Liu De-Pei Wu Jing-Bo wang Xi Zhang heng-xiang wang Feng Gao Shun-Qing wang Zi-Min Sun Jian Ouyang Kai-Lin Xu Su-Jun Gao Lan-Ping Xu Chen-Hua Yan Xiao-Jun Huang Department of Hematology Peking University People’s HospitalPeking University Institute of HematologyNational Clinical Research Center for Hematologic DiseaseBeijing Key Laboratory of Hematopoietic Stem Cell TransplantationBeijing 100044China Department of Hematology Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China Department of Hematology the First Affiliated Hospital of Soochow UniversitySuzhou 215006China Department of Hematology Aero Center Space HospitalBeijing 100049China Department of Hematology Xinqiao HospitalThird Military Medical UniversityChongqing 400037China Department of Hematology Air Force General HospitalPLABeijing 100142China Department of Hematology the Affiliated Hospital of North China University Science and TechnologyTangshan 063210China Department of Hematology Guangzhou First People’s HospitalGuangzhou 510180China Department of Hematology Anhui Provincial HospitalHefei 230001China Department of Hematology Nanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China Department of Hematology the Affiliated Hospital of Xuzhou Medical UniversityXuzhou 221000China Department of Hematology the First Affiliated Hospital of Jilin UniversityChangchun 130021China Peking-Tsinghua Center for Life Sciences Beijing 100871China Research Unit of Key Technique for Diagnosis and Treatments of Hematologic Malignancies Chinese Academy of Medical Sciences2019RU029Beijing 100730China Collaborative Innovation Center of Hematology Beijing 100044China

Prophylactic/preemptive donor lymphocyte infusion(p/pDLI)and intensified conditioning have shown promising results in experimental studies of refractory/relapsed acute leukemia(RRAL),but real-world data remain *** conducted a multicenter,population-based analysis of 932 consecutive *** three-year leukemia-free survival(LFS)rates were 56%for patients receiving both p/pDLI and intensified myeloablative conditioning(MAC)(intenseMAC)and 30%for those who received neither therapy per landmark *** analyses were run separately for acute myeloid leukemia(AML)and acute lymphoblastic leukemia(ALL),and p/pDLI treatment was linked to significantly higher LFS than non-DLI for both AML and ALL patients without increasing the nonrelapse *** was associated with significantly lower relapse and higher LFS than nonintensified MAC despite higher nonrelapse mortality rates in ALL,while there was no impact of intenseMAC observed in AML.p/pDLI achieved superior outcomes in both matched-sibling donor(MSD)and haploidentical donor transplantation,while intenseMAC only influenced MSD *** suggest that RRAL patients receiving“total therapy”by way of p/pDLI and intensified conditioning treatment have an improved chance for LFS,with p/pDLI being safer with a more extensive impact relative to *** with RRAL can tolerate both interventions and achieve a reasonable outcome.

关键词： donor lymphocyte infusion intensified conditioning refractory/relapsed leukemia haploidentical prophylactic

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Unifying Fixed Time and Prescribed Time Control for Strict-Feedback Nonlinear Systems

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IEEE/CAA Journal of Automatica Sinica 2025年第2期12卷 347-355页

作者： xiang Chen Yujuan wang Yongduan Song the State Key Laboratory of Power Transmission Equipment & System Security and New Technology and School of Automation Chongqing University IEEE

This paper investigates the prescribed-time tracking control problem for a class of multi-input multi-output(MIMO)nonlinear strict-feedback systems subject to non-vanishing uncertainties. The inherent unmatched and non-vanishing uncertainties make the prescribed-time control problem become much more nontrivial. The solution to address the challenges mentioned above involves incorporating a prescribed-time filter, as opposed to a finite-time filter, and formulating a prescribed-time Lyapunov stability lemma(Lemma 5). The prescribed-time Lyapunov stability lemma is based on time axis shifting time-varying yet bounded gain, which establishes a novel link between the fixed-time and prescribed-time control method. This allows the restriction condition that the time-varying gain function must satisfy as imposed in most exist prescribed-time control works to be removed. Under the proposed control method, the desire trajectory is ensured to closely track the output of the system in prescribed time. The effectiveness of the theoretical results are verified through numerical simulation.

关键词： Prescribed-time control prescribed-time filter robust control state feedback strict-feedback systems

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Human iPSC-derived mesenchymal stem cells relieve high blood pressure in spontaneously hypertensive rats via splenic nerve activated choline acetyltransferase-positive cells

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Science China(Life Sciences) 2025年第2期68卷 502-514页

作者： Zhen Zhang Weijun Huang Xiaoran Zhang Zhecun wang Manting Xie Bingbing Xie Yiling wang Xiaoyong Chen Andy Peng xiang Qiuling xiang Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-sen UniversityGuangzhou 510080China Department of Physiology Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510080China Department of Vascular Surgery the First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510062China

Despite substantial advancements in modern medicine,the management of hypertension remains a major *** cell-based therapies have recently demonstrated remarkable efficacy in treating cardiovascular diseases,including ***,the antihypertensive mechanism of mesenchymal stem cells(MSCs)has not been extensively *** study aimed to investigate the role of injected MSCs in regulating blood pressure *** previous study demonstrated that human induced pluripotent stem cellderived mesenchymal stem cells(hiPSC-MSCs)are functional and homogeneous sources for MSC-based *** the injection of hiPSCMSCs,a significant reduction in blood pressure and end target organ inflammation were observed in spontaneously hypertensive rats(SHRs).Cell tracking assays demonstrated that the injected hiPSC-MSCs accumulated in the spleens of the *** injected hiPSC-MSCs accumulated adjacent to the splenic nerve,potentially contributing to the antihypertensive ***,the hiPSC-MSCs released abundant glutamate,which acts as a neuromodulator to activate the splenic sympathetic *** inhibition of glutamate synthesis by siRNA,the ability of hiPSC-MSCs to activate sympathetic nerves was significantly *** addition,the antihypertensive effects of hiPSC-MSCs were eliminated after splenic nerve denervation(SND),underscoring the critical role of the splenic ***,activation of the splenic nerve resulted in increased release of norepinephrine(NE),which increased the number of choline acetyltransferase-positive(ChAT^(+))cells in the spleen and peripheral ***,the acetylcholine(ACh)produced by elevated ChAT+cells could act as a vasodilator,lowering blood pressure and mitigating inflammation in end target *** summary,our findings indicate that hiPSC-MSCs effectively lower blood pressure in hypertension by influencing the splenic nerves and regulating ChAT+*** connection between blood pressur

关键词： hiPSC-MSCs hypertension splenic nerve ChAT+cells

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Research progress of lignin-derived materials in lithium/sodium ion batteries

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Green Energy & Environment 2025年第2期10卷 322-344页

作者： Jingke Zhang hengxue xiang Zhiwei Cao Shichao wang Meifang Zhu Institute of Hybrid Materials National Center of International Research for Hybrid Materials TechnologyNational Base of International Science&Technology CooperationCollege of Materials Science and EngineeringQingdao UniversityQingdao266071China State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai201620China

With the increase of energy consumption,the shortage of fossil resource,and the aggravation of environmental pollution,the development of cost-effective and environmental friendly bio-based energy storage devices has become an urgent *** the second most abundant natural polymer found in nature,lignin is mainly produced as the by-product of paper pulping and bio-refining *** possesses several inherent advantages,such as low-cost,high carbon content,abundant functional groups,and bio-renewable,making it an attractive candidate for the rechargeable battery ***,there has been a surge of research interest in utilizing lignin or lignin-based carbon materials as the components of lithium-ion(LIBs)or sodium-ion batteries(SIBs),including the electrode,binder,separator,and *** review provides a comprehensive overview on the research progress of lignin-derived materials used in LIBs/SIBs,especially the application of lignin-based carbons as the anodes of LIBs/*** preparation methods and properties of lignin-derived materials with different dimensions are systemically discussed,which emphasizes on the relationship between the chemical/physical structures of lignin-derived materials and the performances of LIBs/*** current challenges and future prospects of lignin-derived materials in energy storage devices are also proposed.

关键词： Lignin-based carbons Lithium battery Sodium battery Chemical structure evolution

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Vacuum ammonia stripping from liquid digestate:Effects of pH,alkalinity,temperature,negative pressure and process optimization

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Journal of Environmental Sciences 2025年第3期149卷 638-650页

作者： Qiuhong Chen Donghai Yang xiang Chen Xiankai wang Bin Dong Xiaohu Dai State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji UniversityShanghai 200092China YANGTZE Eco-Environment Engineering Research Center National Engineering Research Center of Eco-environment Protection for Yangtze River Economic BeltChina Three Gorges CorporationWuhan 430010China

High ammonia-nitrogen digestate has become a key bottleneck limiting the anaerobic digestion of organic solid *** ammonia stripping can simultaneously remove and recover ammonia nitrogen,which has attracted a lot of attention in recent *** investigate the parameter effects on the efficiency and mass transfer,five combination conditions(53℃ 15 kPa,60°C 20 kPa,65°C 25 kPa,72°C 35 kPa,and 81°C 50 kPa)were conducted for ammonia stripping of sludge *** results showed that 80%of ammonia nitrogen was stripped in 45 min for all experimental groups,but the ammonia transfer coefficient varied under different conditions,which increased with the rising of boiling point temperature,and reached the maximum value(39.0 mm/hr)at 81°C 50 *** ammonia nitrogen removal efficiency was more than 80%for 30 min vacuum stripping after adjusting the initial pH to above 9.5,and adjustment of the initial alkalinity also affects the pH value of liquid *** was found that pH and alkalinity are the key factors influencing the ammonia nitrogen dissociation and removal efficiency,while temperature and vacuum mainly affect the ammonia nitrogen mass transfer and removal *** terms of the mechanism of vacuum ammonia stripping,it underwent alkalinity destruction,pH enhancement,ammonia nitrogen dissociation,and free ammonia *** this study,two-stage experiments of alkalinity destruction and ammonia removal were also carried out,which showed that the two-stage configuration was beneficial for ammonia *** provides a theoretical basis and practical technology for the vacuum ammonia stripping from liquid digestate of organic solid waste.

关键词： Liquid digestate Vacuum ammonia stripping Alkalinity Mass transfer Process optimization

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Research Progress of Chinese Medicine Monomers in Treatment of Cholangiocarcinoma

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Chinese Journal of Integrative Medicine 2025年第2期31卷 170-182页

作者： wang xiang wang Xiao-qing LUO Kai BAI He QU Jia-lin ZHANG Gui-xin Department of General Surgery the Second Hospital of Dalian Medical UniversityDalianLiaoning Province(116027)China Clinical Laboratory of Integrative Medicine First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning Province(116021)China Hepatobiliary Surgery Department Shandong Provincial Third HospittalShandong UniversityJinan(250031)China Institute(College)of Integrative Medicine Dalian Medical UniversityDalianLiaoning Province(116044)China

Cholangiocarcinoma(CCA)is a malignant tumor originating from ***,it remains unclear about the pathogenesis of this carcinoma,which may be related to multiple ***,CCA is mainly treated by surgery,chemotherapy,and *** them,surgery is the only potentially curative option for ***,the high malignancy and asymptomatic nature of CCA may lead to poor treatment *** has been demonstrated that Chinese medicine(CM)plays a significant role in various antitumor ***,CM exhibits fewer side effects and high ***,the in vitro application of CM monomers has been explored in many domestic and foreign *** article mainly reviews the signaling pathways and molecular mechanisms of CM monomers in the treatment of CCA in recent *** findings are expected to provide new insights into the treatment of CCA.

关键词： cholangiocarcinoma Chinese medicine monomers antitumor signaling pathways molecular mechanisms review

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Chattering-Free Fault-Tolerant Cluster Control and Fault Direction Identification for HIL UAV Swarm With Pre-Specified Performance

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IEEE/CAA Journal of Automatica Sinica 2025年第1期12卷 183-197页

作者： Pei-Ming Liu xiang-Gui Guo Jian-Liang wang Daniel Coutinho Lihua Xie the Beijing Engineering Research Center of Industrial Spectrum Imaging School of Automation and Electrical Engineering University of Science and Technology Beijing the Shunde Graduate School of University of Science and Technology Beijing IEEE Autonomous Intelligent Systems Department Hangzhou Innovation Institute of Beihang University the Department of Automation and Systems Universidade Federal de Santa Catarina the School of Electrical and Electronic Engineering Nanyang Technological University

In this paper, the problem of pre-specified performance fault-tolerant cluster consensus control and fault direction identification is solved for the human-in-the-loop(HIL) swarm unmanned aerial vehicles(UAVs) in the presence of possible nonidentical and unknown direction faults(NUDFs) in the yaw *** control strategy begins with the design of a pre-specified performance event-triggered observer for each individual *** observers estimate the outputs of the human controlled UAVs, and simultaneously achieve the distributed design of actual control signals as well as cluster consensus of the observer *** is worth mentioning that these observers require neither the high-order derivatives of the human controlled UAVs' output nor a priori knowledge of the initial conditions. The fault-tolerant controller realizes the pre-specified performance output regulation through error transformation and the Nussbaum function. It should be pointed out that there are no chattering caused by the jump of the Nussbaum function when a reverse fault occurs. In addition, to provide a basis for further solving the problem of physical malfunctions, a fault direction identification algorithm is proposed to accurately identify whether a reverse fault has occurred. Simulation results verify the effectiveness of the proposed control and fault direction identification strategies when the reverse faults occur.

关键词： Chattering-free cluster consensus fault direction identification human-in-the-loop (HIL) nonidentical and unknown direction faults (NUDFs) pre-specified performance swarm unmanned aerial vehicles (UAVs)

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How do Notch Shape and Boundary Conditions Affect the Hydro-Mechanical Behavior of Fractured Geomaterials?Findings via the Darcy-Brinkman-Biot Approach

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Journal of Earth Science 2025年第1期36卷 351-356页

作者： Yi-xiang wang Xiao-Pei Guo Ling Xu Shi-Feng Lu Department of Civil Engineering School of Human Settlements and Civil EngineeringXi'an Jiaotong UniversityXi'an 710049China China Construction Eighth Engineering Division Co. Ltd.Southwest BranchChengdu 610041China

0 INTRODUCTION Natural geomaterials,including soils and rocks,are invariably exposed to intricate stress conditions,in addition to water pressure,in various engineering contexts such as foundation treatment,mining,and *** geomaterials frequently contain numerous fractures,which significantly influence hydrological dynamics as water permeates through them,leading to processes like expansion and bifurcation(Shu et al.,2023;Tran and Jha,2021;Lei et al.,2017;wang et al.,2015).

关键词： bifurcation invar mining

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The photo-based treatment technology simultaneously removes resistant bacteria and resistant genes from wastewater

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Journal of Environmental Sciences 2025年第2期148卷 243-262页

作者： Zicong Guo xiang Tang Wenjun wang Zhangxiong Luo Yuxi Zeng Nan Zhou Zhigang Yu Dongbo wang Biao Song Chengyun Zhou Weiping Xiong College of Environmental Science and Engineering and Key Laboratory of Environmental Biology and Pollution Control(Ministry of Education) Hunan UniversityChangsha 410082China Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation College of Resources and EnvironmentFujian Agriculture and Forestry UniversityFuzhou 350002China School of Resources and Environment Hunan University of Technology and BusinessChangsha 410205China Australian Centre for Water and Environmental Biotechnology The University of QueenslandBrisbaneQLD 4072Australia

Because of the recent widespread usage of antibiotics,the acquisition and dissemination of antibiotic-resistance genes(ARGs)were prevalent in the majority of ***,the biological wastewater treatment processes used in wastewater treatment plants have a limited efficiencies of antibiotics resistant bacteria(ARB)disinfection and ARGs degradation and even promote the proliferation of ***,ARB and ARGs in effluent pose potential risks if they are not further *** oxidation is considered a promising disinfection technology,where the photocatalytic process generates many free radicals that enhance the interaction between light and deoxyribonucleic acid(DNA)for ARB elimination and subsequent degradation of *** reviewaims to illustrate the progress of photocatalytic oxidation technology for removing antibiotics resistant(AR)from wastewater in recent *** discuss the sources and transfer of ARGs in *** overall removal efficiencies of ultraviolet radiation(UV)/chlorination,UV/ozone,UV/H_(2)O_(2),and UV/sulfate-radical based system for ARB and ARGs,as well as the experimental parameters and removal mechanisms,are systematically *** contribution of photocatalytic materials based on TiO_(2) and g-C_(3)N_(4) to the inactivation of ARB and degradation of ARGs is highlighted,producingmany free radicals to attack ARB and ARGs while effectively limiting the horizontal gene transfer(HGT)in ***,based on the reviewed studies,future research directions are proposed to realize specific photocatalytic oxidation technology applications and overcome current challenges.

关键词： Antibiotics resistant genes Antibiotics resistant bacteria Photocatalytic oxidation

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