Most tumor cells take up more glucose than normal cells but metabolize glucose via glycolysis even in the presence of normal level of oxygen,a phenomenon known as Warburg *** cells commonly express the embryonic M2 is...
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Most tumor cells take up more glucose than normal cells but metabolize glucose via glycolysis even in the presence of normal level of oxygen,a phenomenon known as Warburg *** cells commonly express the embryonic M2 isoform of pyruvate kinase(PKM2) that may contribute to the metabolism shift from oxidative phosphorylation to aerobic glycolysis and *** we show that PKM2 is acetylated on lysine 305 and this acetylation is stimulated by high glucose ***2 K305 acetylation decreases PKM2 enzyme activity and promotes its lysosomal dependent degradation via chaperone-mediated autophagy(CMA).Acetylation increases PKM2 interaction with HSC70,a chaperone for CMA,and association with *** expression of an acetylation mimetic K305Q mutant accumulates glycolytic intermediates and promotes cell proliferation and tumor *** results reveal a acetylation regulation of pyruvate kinase and the link between lysine acetylation and chaperone-mediated autophagy.
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