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SYNTHESIS　AND　ANTI－HUmAN　ImmUNODEFICIENCY　VIRUS－1（HIV－1）　ACTIVITY　OF　9－（2－PHOSPHONYLmETHOXYETHYL）ADENINE　（1）AND　ITS　REGIOISOmER　3－（2－PHOSPHONYLmETHOXYETHYL）ADENINE（2）

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Chinese Chemical Letters 1995年第8期6卷 669-672页

作者： Liu, XH Wang, L Guan, BH Kong, m Zhang, XQ Tao, PZ Chen, HS Chinese Acad med Sci Inst Mat Med Beijing 100050 Peoples R China Beijing Union med Coll Beijing 100050 Peoples R China Chinese Acad med Sci Inst Med Biotechnol Beijing 100050 Peoples R China

We have synthesized the title compounds(1)and(2).The structure of regioisomer(4).a new acyclic nucleotide,was determined by spectroscopic methods (1H-NmR.13C-NmR.13C-1H HmBC).A by-product(7)was also isolated and ident...

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HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro

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World Journal of Gastroenterology 2006年第30期12卷 4836-4842页

作者： mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd mahmoud m Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy m Redwan maha El-Demellawy moataza H Omran Wael T El-Garf Said A Goueli Department of Biomedical Technology National Research Center Tahrir Street PO 12622 Dokki Cairo Egypt Department of medicinal Chemistry Na- tional Research Center Tahrir Street PO 12622 Dokki Cairo Egypt Department of microbiology and Immunology Faculty of Pharmacy Cairo University Cairo Egypt Genetic Engineering and Biotechnology Research Institute New Borg El-Arab City Alexandria Egypt Department of Pathology School of medicine University of Wisconsin Madison WI United States

AIm： TO establish a cell culture system with longterm replication of hepatitis C virus （HCV） genome and expression of viral antigens in vitro. mETHODS： HepG2 cell line was tested for its susceptibility to HCV by incubation with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various time points during the culture. Culture supernatant was tested for its ability to infect na＇ive cells. The presence of minus （antisense） RNA strand, and the detection of core and E1 antigens in cells were examined by RT-PCR and immunological techniques （flow cytometry and Western blot） respectively. RESULTS： The intracellular HCV RNA was first detected on d 3 after infection and then could be consistently detected in both cells and supernatant over a period of at least three months. The fresh cells could be infected with supernatant from cultured infected cells. Flow cytometric analysis showed surface and intracellular HCV antigen expression using in house made polyclonal antibodies （anti-core, and anti-E1）. Western blot analysis showed the expression of a cluster of immunogenic peptides at molecular weights extended between 31 and 45 kDa in an one month old culture of infected cells whereas this cluster was undetectable in uninfected HepG2 cells. CONCLUSION： HepG2 cell line is not only susceptible to HCV infection but also supports its replication in vitro. Expression of HCV structural proteins can be detected in infected HepG2 cells. These cells are also capable of shedding viral particles into culture media which in turn become infectious to uninfected cells.

关键词： Hepatitis C virus In vitro propagation Genomic replication Gene expression HepG2 cells

Treatment of Visceral Pain Associated with Irritable Bowel Syndrome using Acupuncture: mechanism of Action

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World Journal of Traditional Chinese medicine 2019年第4期5卷 181-186页

作者： Aleksandar Zivaljevic Bin Shi Elisa m.S.Tam Vahideh Toossi New Zealand College of Chinese medicine

Irritable bowel syndrome(IBS)is a relatively common condition characterized by abdominal pain,among other symptoms,that significantly impacts the quality of life of IBS *** treatment of IBS results in limited success,and the focus is placed on relieving patients of some of the symptoms,visceral pain in *** is commonly used as a treatment modality of ***,the debate on whether acupuncture can be effectively used for this purpose is *** this work,we critically review the available literature to establish a potential mechanism of action in treating visceral pain in IBS using *** sources used are Google Scholar,EBSCO,Cochrane Library and Pub med as well as Chinese database *** keywords used in the literature search are"acupuncture,""IBS,""Irritable Bowel Syndrome,"and"visceral pain."We find that the literature strongly indicates that acupuncture,by stimulating points located on the dermatomes proximal to the spinal level of the area where the sympathetic outflow of the particular gut area affected by the pain is,can interfere with the efferent signal that transports information about the noxious stimuli and interrupt the"connectivity"between the gut and brain,and as a final result,reduce or stop IBS *** findings justify that clinical trials are conducted to test the utility of acupuncture in treating abdominal visceral pain in IBS.

关键词： Acupuncture irritable bowel syndrome visceral pain

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膝关节前髁解剖的性别差异

中华骨科杂志 2010年第3期30卷 I0018-I0023页

作者： Thomas K. Fehring, mD Susan m. Odum, med CCRC, Josh Hughes, BS Bryan D. Springer, mD Walter B. Beaver Jr., mD 张伟滨(译) 王青禾(译) Investigation performed at the OrthoCarolina Hip and Knee Center Charlotte North Carolina 上海瑞金医院骨科 200025

背景：膝关节前髁解剖形态存在明显性别差异的观点已使存全膝关节假体设计时专门为女性患者设计了薄型前髁假体。我们通过对自己的患者进行观察，发现前髁的解剖形态变异很大，不仅取决于患者的性别，还取决于患者的身材、身高和人种。... 详细信息

背景：膝关节前髁解剖形态存在明显性别差异的观点已使存全膝关节假体设计时专门为女性患者设计了薄型前髁假体。我们通过对自己的患者进行观察，发现前髁的解剖形态变异很大，不仅取决于患者的性别，还取决于患者的身材、身高和人种。鉴于所发现的膝关节前髁解剖存在较大变异，我们试图明确是否真正存在前髁解剖的性别差异。方法：我们对212例随机挑选患者的mRI（男112例，女100例）进行评估。前髁定义为膝关节间隙以上10mm的股骨前侧皮质前方的骨性区域。前髁的内、外侧高度直接从mRI两个层面的标记值读出，以毫米为单位。经计算得出的髁高横径比用来确定患者的身材对前髁的大小是否有影响。

关键词：解剖形态性别差异全膝关节女性患者假体设计形态变异膝关节间隙 mRI

Duodenal gangliocytic paraganglioma with lymph node metastases: a case report and comparative review of 31 cases

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World Journal of Clinical Cases 2017年第6期5卷 222-233页

作者： Sahara J Cathcart Aaron R Sasson Jessica A Kozel Jennifer m Oliveto Quan P Ly Department of Pathology and microbiology 985900 Nebraska Medical Center Department of Surgery Division of Surgical Oncology Stony Brook Medicine midwest Pathology Associates LLC Department of Radiology University of Nebraska Medical Center Department of Surgery Division of Surgical Oncology University of Nebraska Medical Center

Gangliocytic paraganglioma(GP) is a rare tumor of uncertain origin most often located in the second portion of the duodenum. It is composed of three cellular components: Epithelioid endocrine cells, spindlelike/sustentacular cells, and ganglion-like cells. While this tumor most often behaves in a benign manner, cases with metastasis are reported. We describe the case of a 62-year-old male with a periampullary GP with metastases to two regional lymph nodes who was successfully treated with pancreaticoduodenectomy. Using Pub med, EmBASE, EBSCOhost medLINE and CINAHL, and Google Scholar, we searched the literature for cases of GP with regional lymph node metastasis and evaluated the varying presentations, diagnostic workup, and disease management of identified cases. Thirty-one cases of GP with metastasis were compiled(30 with at least lymph node metastases and one with only distant metastasis to bone), with age at diagnosis ranging from 16 to 74 years. Ratio of males to females was 19:12. The most common presenting symptoms were abdominal pain(55%) and gastrointestinal bleeding or sequelae(42%). Twenty-five patients underwent pancreaticoduodenectomy. Five patients were treated with local resection alone. One patient died secondary to metastatic disease, and one died secondary to perioperative decompensation. The remainder did well, with no evidence of disease at follow-up from the most recent procedure(except two in which residual disease was deliberately left behind). Of the 26 cases with sufficient histological description, 16 described a primary tumor that infiltrated deep to the submucosa, and 3 described lymphovascular invasion. Of the specific immunohistochemistry staining patterns studied, synaptophysin(SYN) stained all epithelioid endocrine cells(18/18). Neuron specific enolase(NSE) and SYN stained most ganglion-like cells(7/8 and 13/18 respectively), and S-100 stained all spindle-like/sustentacular cells(21/21). Our literature review of published cases of GP

关键词： Gangliocytic paraganglioma metastases duodenum lymph node dissection pancreaticoduodenectomy

HPV16 L1蛋白在大肠杆菌中的表达、纯化及免疫原性研究

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微生物学杂志 2001年第4期21卷 15-16页

作者：商庆龙谷鸿喜哈尔滨医科大学微生物学教研室黑龙江哈尔滨150086

用IPTG诱导目的工程菌 pQE31 HPV16L1/m 15 (pREP4) ,对表达产物进行SDS PAGE和Westernblot分析 ;用表达的L1蛋白免疫BALB/C小鼠得到抗血清后 ,利用真核源性的VLP粗提物验证小鼠抗血清的特异性。利用ImAC金属亲和层析柱纯化L1蛋白。SDS ... 详细信息

用IPTG诱导目的工程菌 pQE31 HPV16L1/m 15 (pREP4) ,对表达产物进行SDS PAGE和Westernblot分析 ;用表达的L1蛋白免疫BALB/C小鼠得到抗血清后 ,利用真核源性的VLP粗提物验证小鼠抗血清的特异性。利用ImAC金属亲和层析柱纯化L1蛋白。SDS PAGE结果显示表达产物在约 5 7ku处有蛋白条带 ;Westernblot结果证实此条带可与HPV16L1蛋白的单克隆抗体反应 ;纯化后的L1蛋白也同样保留免疫特异性 ;小鼠抗血清可与HPV16L1VLP(病毒样颗粒 )发生特异性反应 ,证实重组表达的L1蛋白具有免疫原性。本实验表明HPV16L1蛋白在工程菌m15 (pREP4)中高效表达。

关键词：人乳头瘤病毒16型 L1蛋白表达免疫原性 HPH16 纯化

Fatal and non-fatal injuries due to suspension trauma syndrome: A systematic review of definition, pathophysiology, and management controversies

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World Journal of Emergency medicine 2021年第4期12卷 253-260页

作者： Patrizio Petrone Sofía Espinoza-Villalobos Gerard ABaltazar Kjetil Søreide Adam Stright Collin E.m.Brathwaite D’Andrea K.Joseph Department of Surgery NYU Long Island School of MedicineNYU Langone Hospital–Long IslandMineola 11501USA 2 Department of Gastrointestinal SurgeryStavanger University HospitalStavanger 4011Norway

BACKGROUND: Suspension trauma syndrome is a life-threatening event that occurs when a person is "trapped" in a prolonged passive suspension. It is most commonly seen in people who engage in occupational or sport activities that require harness suspension. The aim of this study is to identify the predisposing factors, pathophysiology, and management of suspension ***: A review and analysis of the literature published in English and Spanish from 1972 to 2020 on suspension trauma were performed. Search sources were Pub med, medline, Cochrane Library, meSH, Up To Date, and Google Scholar. Articles referring to suspension trauma associated with other injury mechanisms(traumatic impact injuries, drowning, asphyxiation, or bleeding), case reports, and pediatric population were ***: Forty-one articles were identified. Of these, 29 articles related to mechanism, pathophysiology, and management of individuals who suffered prolonged suspension trauma without associated traumatic injuries were included in the study. We encountered several controversies describing the putative pathophysiology, ranging from blood sequestration in the lower extremities versus accumulation of metabolic waste and hyperkalemia to dorsal hook-type harness as a trigger cause of positional asphyxia;to vascular compression of femoral vessels exerted by the harness causing decreased venous return. Pstients suspended in a full-body harness with dorsal hook showed more hemodynamic alterations in response to the compressive effect on the rib cage, causing a reduction in perfusion by presenting a decrease in pulse pressure. management strategies varied across ***: Progress has been made in individualizing the population at risk and in the management of suspension trauma. We recommend the formation of consensus definitions, larger cohort or registry studies to be conducted, and experimental animal models to better understand the mechanisms in order to develop management

关键词： Suspension trauma Reflux syndrome Suspension trauma syndrome Harness suspension

Comparison of Cox model methods in A Low-dimensional Setting with Few Events

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Genomics, Proteomics & Bioinformatics 2016年第4期14卷 235-243页

作者： Francisco m. Ojeda Christian miiller Daniela Bornigen David-Alexandre Tregouet Arne Schillert matthias Heinig Tanja Zeller Renate B. Schnabel Department of General and Interventional Cardiology University Heart Center Hamburg-Eppendorf 20246 Hamburg Germany German Center for Cardiovascular Research (DZHK) Hamburg |Kiel| Luebeck Germany Sorbonne Universites University Pierre et marie Curie Paris 06 Institut National pour la Sante et la Recherche Medicale (INSERM) Unite Mixte de Recherche en Sante (UMR_S) 1166 F-75013 Paris France Institute for Cardiometaholism and Nutrition (ICAN) F-75013 Paris France Institut fur medizinische Biometrie und Statistik Universitat zu Lubeck Universitatsklinikum Schleswig-Holstein Campus Lubeck 23562 Lubeck Germany Institute of Computational Biology German Research Center for Environmental Health Helmholtz Zentrum Munchen 85764 Neuherberg Germany

Prognostic models based on survival data frequently make use of the Cox proportional hazards model. Developing reliable Cox models with few events relative to the number of predictors can be challenging, even in low-dimensional datasets, with a much larger number of observations than variables. In such a setting we examined the performance of methods used to estimate a Cox model, including （i） full model using all available predictors and estimated by standard techniques, （ii） backward elimination （BE）, （iii） ridge regression, （iv） least absolute shrinkage and selection operator （lasso）, and （v） elastic net. Based on a prospective cohort of patients with manifest coronary artery disease （CAD）, we performed a simulation study to compare the predictive accuracy, calibration, and discrimination of these approaches, Candidate predictors for incident cardiovascular events we used included clinical variables, biomarkers, and a selection of genetic variants associated with CAD. The penalized methods, i.e., ridge, lasso, and elastic net, showed a comparable performance, in terms of predictive accuracy, calibration, and discrimination, and outperformed BE and the full model. Excessive shrinkage was observed in some cases for the penalized methods, mostly on the simulation scenarios having the lowest ratio of a number of events to the number of variables. We conclude that in similar settings, these three penalized methods can be used interchangeably. The full model and backward elimination are not recommended in rare event scenarios.

关键词： Proportional hazards regression Penalized regression Events per variable Coronary artery disease

A functional PES membrane for hemodialysis-Preparation,characterization and biocompatibility

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Chinese Journal of Chemical Engineering 2015年第7期23卷 1236-1244页

作者： Noel Jacob Kaleekkal A.Thanigaivelan m.Tarun D.mohan membrane Laboratory Department of Chemical Engineering Anna University

In this work, we evaluate the properties of solution casted polysulfone （PSf）/sulfonated polyethersulfone （SPES） blend membranes prepared by non-solvent induced phase inversion technique. The morphologies of these blend membranes, observed using scanning electron microscopy （SEm） and atomic force microscopy （AFm） imaging, indicated a smoother skin layer and an increased number of highly interconnected pores in the sub layer. The efficacy of the prepared membranes was evaluated in terms of porosity, ultrafiltration rate （UFR）, molecular weight cut-off （mWCO） and mean pore size. The hydrophilicity of these membranes was in consonance with contact angle values. It was observed that the selectivity and the UFR of the blend membranes were higher when compared to pristine membranes. Furthermore, these blend membranes demonstrated an increase in biocompatibility - prolonged blood clotting time, suppressed platelet adhesion, reduced protein adsorption and lower complement activation. These membranes were also investigated for uremic solute removal. Diffusive permeability of middle molecular weight cytochrome-c revealed an increase from 8 × 10 ^-4 cm·s ^-1 to 18 × 10^-4 cm· s^- and illustrates the possibility that these sulfonated PES/PSf blend membranes can be used to prepare membrane modules for hemodialysis applications.

关键词： Sulfonated polyethersulfone Hydrophilicity Diffusive permeability Biocompatibility molecular weight cut-off