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Super-resolution immunohistochemistry study on CD4 and CD8 cells and the relation to macrophages in human cochlea

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Journal of Otology 2019年第1期14卷 1-5页

作者： Wei Liu helge rask-andersen Department of Surgical Sciences Section of OtolaryngologyUppsala University Hospital

Recently, the human cochlea has been shown to contain numerous resident macrophages under steady-state. The macrophages accumulate in the stria vascularis, among the auditory nerves, and are also spotted in the human organ of Corti. These macrophages may process antigens reaching the cochlea by invasion of pathogens and insertion of CI electrode. Thus, macrophages execute an innate, and possibly an adaptive immunity. Here, we describe the molecular markers CD4 and CD8 of T cells, macrophage markers MHCⅡ and CD11b, as well as the microglial markers TEME119 and P2Y12, in the human cochlea. Immunohistochemistry and the advantageous super-resolution structured illumination microscopy(SR-SIM) were used in the study. CD4^+ and CD8^+ cells were found in the human cochleae. They were seen in the modiolus in a substantial number adjacent to the vessels, in the peripheral region of the Rosenthal's canal, and occasionally in the spiral ligament. While there are a surprisingly large number of macrophages in the stria vascularis as well as between the auditory neurons,CD4^+ and CD8^+ cells are hardly seen in these areas, and neither are seen in the organ of Corti. In the modiolus,macrophages, CD4^+ and CD8^+ cells appeared often in clusters. Interaction between these different cells was easily observed with SR-SIM, showing closely placed cell bodies, and the processes from macrophages reaching out and touching the lymphocytes. Otherwise the CD4^+ and CD8^+ cells in human cochlear tissue are discretely scattered. The possible roles of these immune cells are speculated.

关键词： Macrophage Human cochlea CD4 CD8 Lymphocyte T cell

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经蜗窗入路的电声联合刺激

中国医学文摘（耳鼻咽喉科学） 2011年第2期26卷 85-87页

作者：刘伟 helge rask-andersen Paul Van de Heyning 李鹏瑞典乌普萨拉大学医院耳鼻咽喉科比利时安特卫普大学医院耳鼻咽喉科中山大学附属第三医院耳鼻咽喉头颈外科

1定义和装置的演变电声联合刺激（electric acoustic stimulation，EAS）是将人工耳蜗电极的电刺激与非电极的声音刺激结合用于同侧耳以恢复听力的技术。该技术已作为常规方法用于中低频率范围听力正常或仅呈中度损失但在更高频率呈陡... 详细信息

1定义和装置的演变电声联合刺激（electric acoustic stimulation，EAS）是将人工耳蜗电极的电刺激与非电极的声音刺激结合用于同侧耳以恢复听力的技术。该技术已作为常规方法用于中低频率范围听力正常或仅呈中度损失但在更高频率呈陡降型重度至极重度听力损失患者的听力康复。具有这种听力图的患者单用传统的助听器受益甚微，因为传统助听器对语音理解至关重要的高频辅音的听力难以提高。

关键词：电刺激(Electric Stimulation) 声刺激(Acoustic stimulation) 耳蜗植入术(Cochlear Implantation) 圆窗(Round Window) ,螺旋神经节(Spiral Ganglion):听觉丧失,感音神经性(Hearing Loss,Sensorineural)

梅尼埃病的影像学评估:球囊耳石可能是病因?

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临床耳鼻咽喉头颈外科杂志 2013年第15期27卷 839-843页

作者： HIDEO YAMANE KISHIKO SUNAMI HIROYOSHI IGUCHI HIRAMORI SAKAMOTO TOSHIO IMOTO helge rask-andersen 张甦琳孔维佳 Department of Otorhinolaryngology Osaka City University Graduate School of MedicineOsakaJapan Department of Otorhinolaryngology Uppsala University HospitalUppsalaSweden 华中科技大学同济医学院附属协和医院耳鼻咽喉科武汉430022

目的:本研究拟采用3DCT来评估梅尼埃病(MD)患者的连合管(RD)、球囊管(SD)和内淋巴囊(ES)的显影情况。方法:根据美国耳鼻咽喉头颈外科学会听力与平衡委员会的诊断标准,我们采用3DCT对62例确诊为单侧MD的患者进行影像学评估,并与其对侧耳... 详细信息

目的:本研究拟采用3DCT来评估梅尼埃病(MD)患者的连合管(RD)、球囊管(SD)和内淋巴囊(ES)的显影情况。方法:根据美国耳鼻咽喉头颈外科学会听力与平衡委员会的诊断标准,我们采用3DCT对62例确诊为单侧MD的患者进行影像学评估,并与其对侧耳及正常对照组(26耳)进行比较,观察它们在3DCT影像中RD、SD和ES的通畅性。结果:MD患耳在3DCT影像中表现为连续性中断,与对照组比较差异有统计学意义(P<0.01)。结论:3DCT成像中,MD耳与对照耳相比,RD、SD和ES的显影明显减弱,提示内淋巴流动可能被致密物质阻断,该物质可能为球囊上脱落的耳石。RD、SD和ES可能参与MD的发病。

关键词：球囊管球囊内淋巴积水内淋巴管 CT成像双侧

MORPHOLOGICAL STUDY OF SURGICALLY OBTAINED HUMAN COCHLEAR SPECIMENS-TECHNICAL ASPECTS

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Journal of Otology 2014年第1期9卷 52-57页

作者： LIU Wei GUO Rui rask ersen helge Department of ORL Section for OtosurgerySurgical Science Institute Uppsala University Dept. Otorhinolaryngology of The Air Force General Hospital

Object To explore the procedures in per-operative harvesting and management of fresh human cochlear specimens for research. Methods During trans-cochlear surgery to remove large petro-clival meningiomas causing life-threatening compression on the brainstem, cochleae are normally destroyed and drilled away in order to reach the apical petrous and clivus region. Instead the cochlea can be dissected out after ethical per-mission was obtained from the local ethical committee (EPN) and allowance gained from the patients. Sur-gery is performed by a team consisting of oto-and neurosurgeons as a two-day procedure with total petro-sectomy in combination with an inferior re-routing of the facial nerve. Fixation of the cochleae was done in the operating room as soon as the specimens had been separated from the temporal bones. Decalcification began after hours’to overnight’s fixation for 4 weeks. Sectioning parallel to the modiolus (mid-modiolus) was performed with a cryostat microtome. The sections were subjected to immunofluorescence (IF). Results Using freshly prepared 4%paraformaldehyde (PFD) solution, adequate fixation of fine inner ear structures was achieved with hours’immersion of the cochlear specimens. Decalcification in 6.2% ethylene di-amine-tetracetic acid (EDTA) solution for 4 weeks yielded a thoroughly decalcified cochlea. Experiences in processing 14 human cochleae and analysing main landmarks in five human inner ear plastic/silicone casts showed that the oval window/stapes footplate are backward tilted, at an angle about 15 degrees, from the plane perpendicular to the modiolar axis. The distance from the modiolar apex to the anterior border of the oval window/footplate in these inner ear casts measured between 4 and 5 mm. High quality IF staining was obtained. Conclusion Surgically obtained human cochlear specimen, when properly processed, contains ide-ally preserved antigenicity for immunohistochemical study. Adequate orientation during sectioning helps ob-tai

关键词： Humancochlea Surgery Petro-clivalmeningioma Morphologicalstudy Immunohistochemistry

Bone mineral density in lifelong trained male football players compared with young and elderly untrained men

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Journal of Sport and Health Science 2018年第2期7卷 159-168页

作者： Marie Hagman Eva Wulff helge Therese Hornstrup Bjorn Fristrup Jens Jung Nielsen Niklas Rye Jorgensen Jesper Lovind andersen Jorn Wulff helge Peter Krustrup Department of Sports Science and Clinical Biomechanics SDU Sport and Health Sciences Cluster (SHSC) University of Southern Denmark Odense 3450 Denmark Department of Nutrition Exercise and Sports University of Copenhagen Copenhagen N 2200 Denmark Institute of Sports Medicine Copenhagen Bispebjerg Hospital Copenhagen NV 2400 Denmark Department of Clinical Biochemistry Rigshospitalet Research Center for Ageing and Osteoporosis Glostrup 2600 Denmark Center of Healthy Aging Department of Biomedical Sciences University of Copenhagen Copenhagen N 2200 Denmark Sport and Health Sciences University of Exeter Exeter EX1 2LU UK

Purpose: The purpose of the present controlled cross-sectional study was to investigate proximal femur and whole-body bone mineral density(BMD), as well as bone turnover profile, in lifelong trained elderly male football players and young elite football players compared with untrained age-matched ***: One hundred and forty healthy, non-smoking men participated in the study, including lifelong trained football players(FTE, n = 35)aged 65—80 years, elite football players(FTY, n = 35) aged 18—30 years, as well as untrained age-matched elderly(UE, n = 35) and young(UY,n = 35) men. All participants underwent a regional dual-energy X-ray Absorptiometry(DXA) scan of the proximal femur and a whole-body DXA scan to determine BMD. From a resting blood sample, the bone turnover markers(BTMs) osteocalcin, carboxy-terminal type-1 collagen crosslinks(CTX-1), procollagen type-1 amino-terminal propeptide(P1NP), and sclerostin were ***: FTE had 7.3%—12.9% higher(p < 0.05) BMD of the femoral neck, wards, shaft, and total proximal femur in both legs compared to UE,and 9.3%—9.7% higher(p < 0.05) BMD in femoral trochanter in both legs compared to UY. FTY had 24.3%—37.4% higher(p < 0.001) BMD in all femoral regions and total proximal femur in both legs compared to UY. The whole-body DXA scan confirmed these results, with FTE showing similar whole-body BMD and 7.9% higher(p < 0.05) leg BMD compared to UY, and with FTY having 9.6% higher(p < 0.001) wholebody BMD and 18.2% higher(p < 0.001) leg BMD compared to UY. The plasma concentration of osteocalcin, CTX-1, and P1NP were 29%,53%, and 52% higher(p < 0.01), respectively, in FTY compared to ***: BMD of the proximal femur and whole-body BMD are markedly higher in lifelong trained male football players aged 65—80 years and young elite football players aged 18—30 years compared to age-matched untrained men. Elderly football players even show higher BMD in femoral trochanter and leg BMD than untrained young despi

关键词： Bone mass Bone turnover markers Dual-energy X-ray absorptiometry Proximal femur bone mineral density Soccer Whole-body bone mineral density

Fitness and health benefits of team handball training for young untrained women--A cross-disciplinary RCT on physiological adaptations and motivational aspects

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Journal of Sport and Health Science 2018年第2期7卷 139-148页

作者： Therese Hornstrup Johan M.Wikman Bjorn Fristrup Susana Povoas Eva W.helge Signe H.Nielsen Jorn W.helge Jesper L.andersen Lars Nybo Peter Krustrup Department of Nutrition Exercise and Sports University of Copenhagen Copenhagen N 2200 Denmark Research Center in Sports Sciences Health Sciences and Human Development CIDESD University Institute of Maia ISMAI Maia 4475-690 Portugal Center of Healthy Aging Department of Biomedical Sciences University of Copenhagen Copenhagen N 2200 Denmark Institute of Sports Medicine Copenhagen Bispebjerg Hospital Copenhagen NV 2400 Denmark Department of Sports Science and Clinical Biomechanics SDU Sport and Health Sciences Cluster (SHSC) University of Southern Denmark Odense 3450 Denmark Sport and Health Sciences University of Exeter Exeter EX1 2LU UK

Purpose: The present study evaluated the effects of regular participation in small-sided team handball training on body composition, osteogenic response, physical performance, and cardiovascular risk factors, as well as well-being and motivation, in young untrained ***: Twenty-eight untrained 20- to 30-year-old women were randomized to a handball training group(HG; n = 14, height 170 ± 5 cm, weight 73 ± 11 kg, VO_(2peak) 37.7 ± 4.1 mL/min/kg) that trained 1.7 ± 0.3 times per week over 12 weeks(70 min 4 v 4 handball sessions) or an inactive control group(CG; n = 14, 169 ± 5 cm, 71 ± 12 kg, 38.1 ± 3.7 mL/min/kg). Physiological and psychological and motivational training adaptations were assessed pre-and post-intervention by dual-energy X-ray Absorptiometry(DXA) scans, blood sampling, physical tests, and ***: The average heart rate(HR) over all training sessions was equal to 85% ± 6% HR_(max). Between-group intervention effects were observed in favor of HG for muscle mass(2.1%, p = 0.024), proximal femur bone mineral density(0.8%, p = 0.041), Yo-Yo IE1 intermittent endurance test level 1(IE1) performance(35%, p < 0.001), and incremental treadmill test performance(11.5%, p = 0.003), but not total fat mass(p = 0.176),mean arterial blood pressure(p = 0.328), resting HR(p = 0.219), or blood lipids(p = 0.298—0.854). In CG, no changes were observed in any of the measured physiological variables after the training period. Compared to CG, HG had an increase in intrinsic motivation(p < 0.001) and in the well-being subscale "energy"(p = 0.010).Conclusion: Participation in regular recreational team handball training organized as small-sided games has marked beneficial effects on physical performance, musculoskeletal fitness, well-being, and motivation in untrained young women.

关键词： Bone mineral density (BMD) Intensity Intermittent Motivation Muscle mass Physical performance Recreational handball Well-being

HMG：心脏病发作的记忆储存在我们的基因中

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现代生物医学进展 2016年第31期16卷 I0004-I0004页

作者： Mathias rask-andersen David Martinsson Muhammad Ahsan Stefan Enroth Weronica E. Ek Ulf Gyllensten sa Johansson 1 Department of Immunology Genetics and Pathology Uppsala University Uppsala Sweden * To whom correspondence should be addressed at: Mathias Rask-Andersen Department of Immunology Genetics and Pathology Uppsala University BMC Husargatan 3 Box 815 751 08 Uppsala Sweden. Tel: +46(0)735345475 Email: mathias.rask-andersen@igp.uu.se Search for other works by this author on: Oxford Academic PubMed Google Scholar 1 Department of Immunology Genetics and Pathology Uppsala University Uppsala Sweden Search for other works by this author on: Oxford Academic PubMed Google Scholar 1 Department of Immunology Genetics and Pathology Uppsala University Uppsala Sweden Search for other works by this author on: Oxford Academic PubMed Google Scholar 1 Department of Immunology Genetics and Pathology Uppsala University Uppsala Sweden Search for other works by this author on: Oxford Academic PubMed Google Scholar 1 Department of Immunology Genetics and Pathology Uppsala University Uppsala Sweden Search for other works by this author on: Oxford Academic PubMed Google Scholar 1 Department of Immunology Genetics and Pathology Uppsala University Uppsala Sweden Search for other works by this author on: Oxford Academic PubMed Google Scholar 1 Department of Immunology Genetics and Pathology Uppsala University Uppsala Sweden Search for other works by this author on: Oxford Academic PubMed Google Scholar

遗传因素和环境因素影响我们的心血管疾病风险。如今。在一项新的研究中，来自瑞典乌普萨拉大学的研究人员发现心脏病发作的记忆能够通过表观遗传变化储存在我们的基因中。相关研究结果在线发表在Human Molecular Genetics期刊上。

关键词：心脏病基因储存记忆 HMG 心血管疾病 Human 环境因素