检索结果-南通市图书馆

使用鲑鱼脑垂体提液、促性腺激素释放激素和抗雌激素对银大麻哈鱼(Oncokhynchus kisutch)进行催产

在线全文

学校读者我要写书评

暂无评论

国外水产 1980年第4期 44-44页

作者： Edward m.Donaldson George Hunter helen m.Dye 熊笑园

从Big Qualicum孵化场将已达性成熟的三龄雌性银大麻哈鱼运到实验室,放入直径为3米的水池中,每分钟注入淡水60升,水温为10.5℃,水深为0.8米。这些鲑鱼都分别作了标记,并任意分为十一组。在0天注:(即第一天的前一天,1977年11月4日)和第3... 详细信息

从Big Qualicum孵化场将已达性成熟的三龄雌性银大麻哈鱼运到实验室,放入直径为3米的水池中,每分钟注入淡水60升,水温为10.5℃,水深为0.8米。这些鲑鱼都分别作了标记,并任意分为十一组。在0天注:(即第一天的前一天,1977年11月4日)和第3天(1977年11月7日),在二苯氧基乙醇麻醉下,按下述情况对这十一组鲑鱼进行了腹腔注射(剂量:毫克/公斤体重)。 (1)葡萄糖盐液/葡萄糖盐液。 (2)葡萄糖盐液/花生油与注射tamoxifen的鱼组进行对照;

关键词：银大麻哈鱼 Oncokhynchus kisutch 鱼卵卵子提液脑垂体内分泌腺

Separation of Soybean Oil/n-hexane miscellas Using Polymeric membranes

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Food Science and Engineering 2012年第11期2卷 616-624页

作者： marcus V. Tres Jéssica C. Racoski Zuleica Novello Jonas R.m. de melo helen C. Ferraz marco DiLuccio José Vladimir Oliveira: Department of Food Engineering URI-Campus de Erechim Av. Sete de Setembro 1621 Erechim 99700-000 RS Brazil Program of Chemical Engineering COPPE UFRJ-Centro de Tecnologia Bloco G Sala 115 Rio de Janeiro 21941-972 R JBrazil

Asymmetrical positive assortative mating induced by developmental lead （Pb2＋） exposure in a model system, Drosophila melanogaster

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Current Zoology 2017年第2期63卷 195-203页

作者： Elizabeth K. PETERSON Roman YUKILEVICH Joanne KEHLBECK Kelly m. LARUE Kyle FERRAIOLO Kurt HOLLOCHER Helmut V.B. HIRSCH Bernard POSSIDENTE Department of Biological Sciences State University of New York at Albany Albany NY 12222 USA Department of Biology Union College Schenectady NY 12308 USA Department of Chemistry Union College Schenectady NY 12308 USA Department of molecular Biology Princeton University Princeton NJ 08544 USA Department of Geology Union College Schenectady NY 12308 USA Department of Biology Skidmore College Saratoga Springs NY 12866 USA

Anthropogenic pollutants have the potential to disrupt reproductive strategies. Little is known about how lead (Pb) exposure disrupts individual-level responses in reproductive behaviors, which are important for fitness. was used as a model system to determine the effects of: 1) developmental lead exposure on pre-mating reproductive behaviors (i.e., mate preference), and 2) lead exposure and mating preferences on fitness in the parental generation and un-exposed offspring. Wild-type strains of were reared from egg stage to adulthood in control or leaded medium (250 μm PbAc) and tested for differences in: mate preference, male song performance, sex pheromone expression, fecundity, mortality, and body weight. leaded females preferentially mated with leaded males (i.e., asymmetrical positive assortative mating) in 2-choice tests. This positive assortative mating was mediated by the females (and not the males) and was dependent upon context and developmental exposure to Pb. Neither the courtship song nor the sex pheromone profile expressed by control and leaded males mediated the positive assortative mating in leaded females. Leaded females did not incur a fitness cost in terms of reduced fecundity, increased mortality, or decreased body weight by mating with leaded males. These results suggest that sublethal exposure to lead during development can alter mate preferences in adults, but not fitness measures once lead exposure has been removed. We suggest that changes in mate preference may induce fitness costs, as well as long-term population and multi-generational implications, if pollution is persistent in the environment.

关键词： cuticular hydrocarbons positive assortative mating random mating species recognition system.

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

儿童持续性部分性癫痫的诊断算法

癫痫杂志 2021年第3期7卷 265-272页

作者： Snehal Surana Thomas Rossor Yael Hacohen Hassell J Boyd S D’Arco F Aylett S Bhate S Carr L Das K DeVile C Eltze C Hemingway C Kaliakatsos m O’Callaghan F Prabhakar P Robinson R Varadkar S helen Cross J Hacohen Y 陈晨(译) 慕洁(审) 不详

总结一组持续性部分性癫痫(Epilepsia partialis continua,EPC)患儿的特点,并提出一种整合关键鉴别诊断的诊断算法。对2002年—2019年在三级儿科神经病学中心就诊的EPC患儿进行分析。54例患儿符合EPC诊断标准。中位发病年龄为7岁(0.6~15... 详细信息

总结一组持续性部分性癫痫(Epilepsia partialis continua,EPC)患儿的特点,并提出一种整合关键鉴别诊断的诊断算法。对2002年—2019年在三级儿科神经病学中心就诊的EPC患儿进行分析。54例患儿符合EPC诊断标准。中位发病年龄为7岁(0.6~15岁),中位随访时间为4.3年(0.2~16年)。其中,诊断为Rasmussen脑炎(Rasmussen encephalitis,RE)30例(56%),线粒体疾病12例(22.2%),磁共振成像(mRI)阳性病灶相关局灶性癫痫6例(11.1%),诊断不明5例(9%)。线粒体疾病患儿更早出现EPC,EPC发病年龄每增加一年,诊断线粒体疾病的几率降低26%(P=0.02)。EPC发作前存在发育问题(OR=22,PmRI提示明显不对称或一侧半球萎缩,其余患儿出现的中位时间则在EPC发作后6个月(3~15个月)。首选诊断检查为头部mRI。一侧半球萎缩结合临床表现及脑电图改变的偏侧性,提示诊断为RE。对于初次扫描时不能诊断为RE,但临床和影像学表现提示RE的儿童,建议每6个月重复影像学检查以排查进行性一侧半球皮质萎缩,并应考虑脑活检,鞘内炎症的证据(寡克隆带和新蝶呤升高)可为佐证。对于存在双侧半球源性EPC的患儿,建议进行快速DNA聚合酶γ(POLG)基因检测,如为阴性,则应提取外周血DNA进行mtDNA测序及全外显子组测序。线粒体病所致EPC患儿表现出与RE和结构性癫痫不同的临床特征。这一针对EPC患儿的诊断算法有助于进行针对性检查并及时诊断。

关键词：自身免疫性癫痫持续性部分性癫痫线粒体疾病 POLG相关癫痫 Rasmussen脑炎

在线全文

学校读者我要写书评

暂无评论

产前和产后双酚A暴露和童年体质指数

环境与职业医学 2013年第7期30卷 554-554页

作者： Kim G.Harley Raul Aguilar Schall Jonathan Chevrier Kristin Tyler helen Aguirre Asa Bradman Nina T.Holland Robert H.Lustig Antonia m.Calafat Brenda Eskenazi

[背景]双酚A(BPA)是一种广泛使用的内分泌干扰化学物,与啮齿类动物体重增加和脂肪沉积有关联。[目的]在CHAmACOS纵向队列的9岁儿童组(n=311)中,研究产前和产后尿BPA浓度与体质指数(BmI)、腰围、体脂肪率和肥胖间的关联。[方法]采集母亲... 详细信息

[背景]双酚A(BPA)是一种广泛使用的内分泌干扰化学物,与啮齿类动物体重增加和脂肪沉积有关联。[目的]在CHAmACOS纵向队列的9岁儿童组(n=311)中,研究产前和产后尿BPA浓度与体质指数(BmI)、腰围、体脂肪率和肥胖间的关联。[方法]采集母亲怀孕期间现场收集的两次尿样以及5岁和9岁儿童的尿样,测量BPA浓度。[结果]产前尿BPA浓度与9岁女孩BmI降低有关联,但在男孩中无此关联。在女孩中,位于产前BPA浓度最高的三分位数与BmI z分值降低(β=-0.47,95%CI:-0.87～-0.07)和体脂肪率降低(β=-4.36,95%CI:-8.37～-0.34)有关联;与位于最低三分位数组的女孩相比,超重/肥胖的风险下降[比值比(OR)=0.37,95%CI:0.16～0.91]。这些发现在青春期前期的女孩中最强。5岁时的尿BPA浓度与5岁或9岁时任何人体测量学参数没有相关性,在9岁时的BPA浓度与男孩和女孩9岁时的BmI、腰围、脂肪量、超重/肥胖呈正相关。[结论]本研究的发现与其他横断面研究相一致,9岁时尿BPA浓度较高与9岁时肥胖增加相关联。然而,母亲怀孕期间BPA浓度增加与他们的女儿9岁时BmI、体内脂肪和超重/肥胖降低有关联。

关键词：双酚A BmI CHAmACOS 儿童肥胖

Overcoming the challenges of cancer drug resistance through bacterial-mediated therapy

在线全文

学校读者我要写书评

暂无评论

Chronic Diseases and Translational medicine 2019年第4期5卷 258-266页

作者： Amin Zargar Samantha Chang Ankita Kothari Antoine m.Snijders Jian-Hua mao Jessica Wang Amanda C.Hernandez Jay D.Keasling Trever G.Bivona Joint BioEnergy Institute Lawrence Berkeley National LaboratoryEmeryvilleCA 94608USA QB3 Institute University of California-Berkeley174 Stanley HallBerkeleyCA 94720USA Biological Systems and Engineering Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA Department of Chemical&Biomolecular Engineering Department of BioengineeringUniversity of CaliforniaBerkeleyCA 94720USA helen Diller Family Comprehensive Cancer Center University of CaliforniaSan FranciscoCA 94143USA Department of Cellular and molecular Pharmacology University o f CaliforniaSan FranciscoCA 94143USA

Despite tremendous efforts to fight cancer,it remains a major public health problem and a leading cause of death *** increased knowledge of cancer pathways and improved technological platforms,precision therapeutics that specifically target aberrant cancer pathways have improved patient ***,a primary cause of unsuccessful cancer therapy remains cancer drug *** this review,we summarize the broad classes of resistance to cancer therapy,particularly pharmacokinetics,the tumor microenvironment,and drug resistance ***,we describe how bacterial-mediated cancer therapy,a bygone mode of treatment,has been revitalized by synthetic biology and is uniquely suited to address the primary resistance mechanisms that confound traditional *** genetic engineering,we discuss how bacteria can be potent anticancer agents given their tumor targeting potential,anti-tumor activity,safety,and coordinated delivery of anti-cancer drugs.

关键词： Cancer therapy Synthetic biology Drug resistance Bacterial-mediated therapy

mobilizing Crop Biodiversity

在线全文

学校读者我要写书评

暂无评论

molecular Plant 2020年第10期13卷 1341-1344页

作者： Susan mcCouch Zahra Katy Navabi michael Abberton Noelle L.Anglin Rosa Lia Barbieri michael Baum Kirstin Bett helen Booker Gerald L.Brown Glenn J.Bryan Luigi Cattivelli David Charest Kellye Eversole marcelo Freitas Kioumars Ghamkhar Dario Grattipaglia Robert Henry maria Cleria Valadares Inglis Tofazzal Islam Zakaria Kehel Paul J.Kersey Graham J.King Stephen Kresovich Emily marden Sean mayes marie Noelle Ndjiondjiop Henry T.Nguyen Samuel Rezende Paiva Roberto Papa Peter W.B.Phillips Awais Rasheed Christopher Richards mathieu Flouard maria Jose Amstalden Sampaio Uwe Scholz Paul D.Shaw Brad Sherman S.Evan Staton Nils Stein Jan Svensson mark Tester Jose Francisco montenegro Valls Rajeev Varshney Stephen Visscher Eric von Wettberg Robbie Waugh Peter Wenzl Loren H.Rieseberg Plant Breeding and Genetics School of Integrated Plant SciencesCornell UniversityIthacaNY14853USA DivSeek Global Institute for Food Security110 Gymnasium PlaceUniversity of SaskatchewanSaskatoonSKS7N 0W9Canada lnternational Institute of Tropical Agriculture(IITA) PMB 5320Oyo RdIbadanNigeria International Potato Center(CIP)1895 Avenida La molina Lima Peru 12Lima 15023Peru Embrapa Genetic Resources and Biotechnology Parque Estacjao BiologicaFinal Av W5 NorteCaixa Postal 0237270770-917-Brasilia DFBrazil International Center for Agricultural Research in the Dry Areas(I CAR DA) Station Exp.INRA-Quich.Rue Hafiane Cherkaoui.Agdal.Rabat-Instituts10111RabatMorocco Department of Plant Sciences University of Saskatchewan51 Campus Dr.SaskatoonSK S7N 5A8Canada Department of Plant Agriculture University of GuelphRm 316Crop Science Bldg50 Stone Rd EGuelphON N1G 2W1Canada Genome Prairie 111 Research DriveSuite 101SaskatoonSKS7N 3R2Canada The James Hutt on Institute Errol RoadInvergowrieDundeeDD25DAUK CREA Research Centre for Genomics and Bioinformaticsvia San Protaso 302Fiorenzuola d'Arda29017Italy Genome British Columbia 400-575 West 8th AvenueVancouverBCV5Z 0C4Canada International Wheat Genome Sequencing Consortium 2841 NE Marywood CtLee's SummitMO.64086USA Forage Science Grasslands Research CentreAgResearchPalmerston North4410New Zealand Queensla nd Alliance for Agriculture and Food Inn ovation University of QueenslandBrisbaneQLD 4072Australia Institute of Biotechnology and Genetic Engineering(IBGE) Bangabandhu Sheikh Mujibur Rahman Agricultural UniversityGazipur 1706Bangladesh Southern Cross University PO Box 157LismoreNSW 2480Australia Royal Botanic Gardens KewRichmondSurreyTW93AEUK Feed the Future Innovation Lab for Crop Improvement 431 Weill HallCornell UniversityIthacaNY14853USA Department of Botany and Biodiversity Research Centre University of British ColumbiaVancouverBC V6R 2A5Canada Crops For the Future(UK)CIC 76-80 Baddow Road ChelmsfordEs

Over the past 70 years,the world has witnessed extraordinary growth in crop productivity,enabled by a suite of technological advances,including higher yielding crop varieties,improved farm management,synthetic agrochemicals,and agricultural *** this"Green Revolution"intensified crop production,and is credited with reducing famine and malnutrition,its benefits were accompanied by several undesirable collateral effects(Pingali,2012).These include a narrowing of agricultural biodiversity,stemming from increased monoculture and greater reliance on a smaller number of crops and crop varieties for the majority of our *** reduction in diversity has created vulnerabilities to pest and disease epidemics,climate variation,and ultimately to human health(Harlan,1972).

关键词： intensified crops agricultural

Nature：大规模研究揭示生命所必需的基因

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

现代生物医学进展 2016年第31期16卷 I0002-I0003页

作者： Dickinson, mary E. Flenniken, Ann m. Ji, Xiao Teboul, Lydia Wong, michael D. White, Jacqueline K. meehan, Terrence F. Weninger, Wolfgang J. Westerberg, Henrik Adissu, Hibret Baker, Candice N. Bower, Lynette Brown, James m. Caddle, L. Brianna Chiani, Francesco Clary, Dave Cleak, James Daly, mark J. Denegre, James m. Doe, Brendan Dolan, mary E. Edie, Sarah m. Fuchs, Helmut Gailus-Durner, Valerie Galli, Antonella Gambadoro, Alessia Gallegos, Juan Guo, Shiying Horner, Neil R. Hsu, Chih-Wei Johnson, Sara J. Kalaga, Sowmya Keith, Lance C. Lanoue, Louise Lawson, Thomas N. Lek, monkol mark, manuel Arschall, Susan m. mason, Jeremy mcElwee, melissa L. Newbigging, Susan Nutter, Lauryl m. J. Peterson, Kevin A. Ramirez-Solis, Ramiro Rowland, Douglas J. Ryder, Edward Samocha, Kaitlin E. Seavitt, John R. Selloum, mohammed Szoke-Kovacs, Zsombor Tamura, masaru Trainor, Amanda G. Tudose, Ilinca Wakana, Shigeharu Warren, Jonathan Wendling, Olivia West, David B. Wong, Leeyean Yoshiki, Atsushi macArthur, Daniel G. Tocchini-Valentini, Glauco P. Gao, Xiang Flicek, Paul Bradley, Allan Skarnes, William C. Justice, monica J. Parkinson, helen E. moore, mark Wells, Sara Braun, Robert E. Svenson, Karen L. de Angelis, martin Hrabe Herault, Yann mohun, Tim mallon, Ann-marie Henkelman, R. mark Brown, Steve D. m. Adams, David J. Lloyd, K. C. Kent mcKerlie, Colin Beaudet, Arthur L. Bucan, maja murray, Stephen A. Dept mol Physiol & Biophys Houston TX 77030 USA Toronto Ctr Phenogen Toronto ON M5T 3H7 Canada mt Sinai Hosp Toronto ON M5G 1X5 Canada Univ Penn Perelman Sch Med Genom & Computat Biol Program Philadelphia PA 19104 USA med Res Council Harwell Mammalian Genet Unit Harwell OX11 0RD Oxon England mary Lyon Ctr Harwell OX11 0RD Oxon England Hosp Sick Children Mouse Imaging Ctr Toronto ON M5T 3H7 Canada Wellcome Trust Sanger Inst Wellcome Trust Genome Campus Cambridge CB10 1SA England European Bioinformat Inst European Mol Biol Lab Wellcome Trust Genome Campus Cambridge CB10 1SD England med Univ Vienna Ctr Anat & Cell Biol A-1090 Vienna Austria Hosp Sick Children 555 Univ Ave Toronto ON M5G 1X8 Canada Jackson Lab 600 Main St Bar Harbor ME 04609 USA Univ Calif Davis Mouse Biol Program Davis CA 95618 USA Italian Natl Res Council CNR Inst Cell Biol & Neurobiol Monterotondo Mouse Clin I-00015 Monterotondo Italy massachusetts Gen Hosp Analyt & Translat Genet Unit Boston MA 02114 USA Broad Inst mIT & Harvard Program Med & Populat Genet Cambridge MA 02142 USA German Res Ctr Environm Hlth Helmholtz Zentrum Munchen Inst Expt Genet D-85764 Neuherberg Germany German mouse Clin D-85764 Neuherberg Germany Baylor Coll med Dept Mol & Human Genet Houston TX 77030 USA Nanjing Univ Nanjing Biomed Res Inst Collaborat Innovat Ctr Genet & Dev SKL Pharmaceut Biotechnol & Model Anim Res Ctr Nanjing 210061 Jiangsu Peoples R China Univ Strasbourg ICS Infrastruct Natl PHENOMIN F-67404 Illkirch Graffenstaden France Univ Strasbourg INSERM CNRS IGBMC F-67404 Illkirch Graffenstaden France RIKEN BioResource Ctr Tsukuba Ibaraki 3050074 Japan Childrens Hosp Oakland Res Inst Oakland CA 94609 USA ImPC San Anselmo CA 94960 USA Tech Univ munich Sch Life Sci Weihenstephan Chair Expt Genet D-81675 Freising Weihenstephan Germany German Ctr Diabet Res DZD D-85764 Neuherberg Germany Francis Crick Inst Mill Hill Lab Mill Hill London NW1 1AT England Univ Penn Perlman

在哺乳动物基因组中。大约三分之一的基因是生命所必需的。在一项新的研究中，来自美国贝勒医学院等多个机构的研究人员描述了这些基因的大规模发现。以及它将如何影响对哺乳动物发育和人类疾病的理解。相关研究结果在线发表在Nature期... 详细信息

在哺乳动物基因组中。大约三分之一的基因是生命所必需的。在一项新的研究中，来自美国贝勒医学院等多个机构的研究人员描述了这些基因的大规模发现。以及它将如何影响对哺乳动物发育和人类疾病的理解。相关研究结果在线发表在Nature期刊上，论文标题为“High—throughput discovery of novel developmental phenotypes”。

关键词：动物基因组生命哺乳动物研究人员人类疾病医学院

Ym155 Inhibits Neuroblastoma Cell migration and Survival <i>in Vitro</i>and Tumor Growth and metastatic Burden in a Pre-Clinical model

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Cancer Therapy 2014年第13期5卷 1289-1302页

作者： Heather m. Calderone Akshita Dutta Lauren Smith Alexandra Eckardt Ping Zhao Giselle Saulnier Sholler Laboratory for Translational Neuroblastoma Research Van Andel Research Institute Grand Rapids USA Pediatric Oncology Translational Research Program Helen DeVos Children’s Hospital Grand Rapids USA michigan State University College of medicine Grand Rapids USA

Background: Neuroblastoma exhibits a high incidence of chromosomal translocations, the most common being the gain of a portion of the long arm of chromosome 17. This region includes the gene BIRC5/survivin, which is highly upregulated in neuroblastoma and correlates with poor prognosis. Survivin is a member of the inhibitor of apoptosis family of proteins and is involved in tumor cell survival and migration. Ym155 is a small molecule inhibitor of survivin transcription and has shown efficacy in several cancer model systems both?in vitro?and?in vivo. Procedure: Cells were treated with Ym155 and effects on migration, invasion, and apoptosis signaling were investigated?in vitro. Tumor burden was assessed in xenografted mice by measuring tumor volume and liver metastases. Results: Treatment with Ym155 caused a dose-dependent decrease in survivin expression and induction of apoptosis. Lower concentrations of Ym155 reduced cell migration and invasion by 15% - 50% which varied by cell line. In a xenograft model, Ym155 treatment inhibited tumor growth by 25% - 70%, reduced metastatic burden in the liver by 50%, and prolonged animal survival. Conclusion: The data suggest Ym155 as a possible therapeutic agent for metastatic neuroblastoma.

关键词： Neuroblastoma Ym155 Survivin metastasis Apoptosis