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Genetic Diversity of Uropathogenic Escherichia coli Isolated from Human T Lymphotropic Virus Type I (HTLV-I) Infected Individuals

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Advances in Infectious Diseases 2016年第4期6卷 163-173页

作者： Andréia santos Silva Elizabeth de souza Neves Maria Cristina Lourenç o Márcia dos santos Guimarã es Debora Ribeiro de souza santos Adriana Hamond Regua-Mangia Escola Nacional de Saúde Pública Sergio Arouca Funda&ccedil ã o Oswaldo Cruz Rio de Janeiro Brazil Instituto Nacional de Infectologia Funda&ccedil ã o Oswaldo Cruz Rio de Janeiro Brazil Instituto Nacional de Controle de Qualidade em Saúde Funda&ccedil ã o Oswaldo Cruz Rio de Janeiro Brazil

Urinary tract infection (UTI) is a frequent pathology among HTLV-I+ individuals being capable of severely compromising the kidneys and bladder. Molecular characteristics of uropathogenic Escherichia coli (UPEC) from HTLV-I+ infected individuals are unknown. UPEC isolates from HTVL-I+ individuals, with and without clinical symptoms of myelopathy, were submitted to genetic typing seeking to infer bacterial diversity and potential virulence. 71 bacterial isolates were characterized according to random amplified polymorphic DNA and phylotypes. Phylotyping classified E. coli into four phylogenetic groups: A (18.3%), B1 (16.9%), B2 (39.4%), and D (25.3%) and 8 phylotypes according to the presence of the genetic sequences chuA, yjaA and the DNA fragment TSPE4.C2: ﹣﹣﹣ (5.6%) and ﹣+﹣ (12.6%) in phylogroup A, ﹣﹣+ (7.0%) and ﹣++ (9.8%) in B1, +++ (32.3%) and ++﹣ (7.0%) in B2, +﹣﹣ (15.4%) and +﹣+ (9.8%) in D. The B2 phylogroup was the most prevalent among HTLV﹣ associated myelopathy and asymptomatic individuals. RAPD-PCR typing revealed a high degree of bacterial polymorphism indicating a non-clonal origin. Genotypes were not found to be distributed according to clinical status or epidemiological features. Our results lead us to suggest that the neurological impairment in HTLV-I+ individuals can be a risk factor for urinary infections due E. coli which are caused by distinct bacterial lineages.

关键词： Uropathogenic Escherichia coli Genetic Diversity Phylotype HTLV-I+

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Areas with evidence of equity and their progress on mortality from tuberculosis in an endemic municipality of southeast Brazil

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Infectious Diseases of Poverty 2017年第1期6卷 1185-1195页

作者： Mellina YAMAMURA Marcelino santos NETO Francisco CHIARAVALLOTI NETO Luiz Henrique ARROYO Antonio Carlos Vieira RAMOS Ana Angelica Rego de QUEIROZ Aylana de souza BELCHIOR Danielle Talita dos santos Juliane de Almeida CRISPIM Ione Carvalho PINTO Severina Alice da Costa UCHOA Regina Celia FIORATI Ricardo Alexandre ARCENCIO School of Nursing of the University of Sao Paulo Ribeirao Preto Campus(EERP/USP)Ribeirao retoSPBrazil Federal University of Maranhao(UFMA) Sao LuísMABrazil School of Public Health of the University of Sao Paulo(FSP/USP) Sao PauloSPBrazil Federal University of Rio Grande do Norte(UFRN) NatalRNBrazil School of Medicine of the University of Sao Paulo Ribeirao Preto Campus(FMRP/USP)Ribeirao PretoSPBrazil

Background:In Brazil,people still fall ill and die from tuberculosis(TB),and this can be explained by the significant impasse in the equity of distribution of therapeutic resources to the population as a *** aim was to identify geographical areas which have shown progress in terms of equity(of income,schooling and urban occupancy)and test its effect on mortality from TB in a municipality of southeast ***:It is an ecological study considering TB as the basic cause for deaths registered between 2006 and 2013 on the Mortality Information System and other variables obtained through the Demographic Census of the Brazilian Institute of Geography and Statistics(2010).The geographical area for analysis comprised the areas of coverage of the health *** indicators have been constructed through the Principal Component Analysis(PCA).The cases were geocoded and the annual mortality rate from TB was calculated with smoothing using the local empirical Bayesian *** linear regression was then *** was confirmation of the existence of spatial dependence of residue through the application of the Global Moran I test,and application of the Models with Global Spatial Effects,to identify the best standard of spatial ***:The mortality rates ranged from 0.00 to 2.8 deaths per 100,000 people,per *** the PCA,three indicators were constructed,and designated as indicators of income,social inequality,and social *** multiple linear regression,the indicator of social equity was statistically significant(P<0.0001)but had a negative association,an adjusted R^(2)of 28.36%and with spatial dependence(Moran I=0.21,P=0.003455).The best model to deal with existing spatial dependence was the Spatial Lag ***:The better social conditions have shown progress in reducing mortality from TB,thereby reinforcing the achievement of Sustainable Development *** addition,cartography was also applied,which can be replicated

关键词： Tuberculosis Spatial analysis Mortality Cause of death

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Fruit Mass of Carica papayaL. from Cultivars Aliança and THB from the Width and Length of the Fruit

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Agricultural Sciences 2021年第1期12卷 9-17页

作者： Karina Tiemi Hassuda dos santos Vinicius de souza Oliveira Gleyce Pereira santos Jéssica Sayuri Hassuda santos Omar Schmildt Edilson Romais Schmildt Federal University of Espírito Santo Center Noth of Espírito Santo S&#227 o Mateus Espírito Santo Brazil Federal University of Espírito Santo Center for Agricultural Sciences and Engineering Alegre Espírito Santo Brazil

Papaya (Carica papaya L.) is a typical plant with a tropical climate, but also grown in subtropical regions. Using mathematical models well-adjusted allows with good precision to estimate characteristics of interest. The objective was to adjust an equation that estimates the fruit mass for each cultivar of papaya, Aliança and THB, using only one measure, length or width. The experiment was conducted in the municipality of Linhares in the state of Espírito Santo, Brazil. Seedlings were planted on the same day, spaced 3.6 × 1.5 m and in rows side by side. Initially, the equations were modeled, they were linearized and then the covariance analysis was performed in order to verify the possibility of an equation that would serve both cultivars. As the covariance was significant, it was necessary to develop equations for each cultivar. To obtain the growth equations, 350 fruits of cultivar Aliança and 550 of THB were used. The validation was performed with 50 fruits of each. The characteristics evaluated were the largest width (W in mm), the longest fruit length (L, in mm) and the observed mass (OM in g). The equations that best fit were those of the power model that use width (W) as an independent variable.

关键词： Non-Destructive Method Regression Validation Mathematical Equations Growth Modeling

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Numerical Simulation of the Shell-and-Tube Heat Exchanger:Influence of the Lower Flows and the Baffles on a Fluid Dynamics

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Advances in Chemical Engineering and Science 2017年第4期7卷 349-361页

作者： Sebastiao Josédos santos Filho Josedite Saraiva de souza Antonio Gilson Barbosa de Lima Departament of Mechanical Engineering UNINASSAUCampina GrandeBrazil Departament of Mechanical Engineering Federal University of Campina GrandeCampina GrandeBrazil

This project proposes the numerical reproduction development of the water flow in a shell-and-tube heat exchanger 2:1 according to the CLASS C TEMA standard (for moderate operation conditions, with commercial application). With baffles in aluminum and copper tube for the cold fluid flow, the shell is in acrylic, and with thermal analysis efficiencies with regard to the presence or not of the baffles, that is to analyze the efficiency with only, the tubes and the shell and soon after the analysis with tubes and baffles. Heat exchangers are widely used equipment on an industrial and commercial scale, the application of these equipments on an industrial scale represents innovative processing solutions reflecting the processes efficiency, producing significant savings and lower cost, supporting the business success, and consequently offering opportunities, social responsibility, which are the pillars of sustainability. For the development of this work the methodology was used to calculate effectiveness, following the design parameters and contour conditions, it was analyzed the fluids behavior in the shell and the tube, through computational fluid dynamics (CFD) using the software ANSYS CFX 15.0. The results were compared with Excel generated worksheets calculated using the existing equations and correlations.

关键词： Heat Exchanger CFD Baffles ANSYS CFX

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Molecular and serology methods in the diagnosis of COVID-19: An overview

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World Journal of Methodology 2022年第3期12卷 83-91页

作者： Marcel Silva Luz Ronaldo Teixeira da Silva Júnior Gabriella Almeida santos de Santana Gabriela santosRodrigues Henrique de Lima Crivellaro Mariana santos Calmon Clara Faria souza Mendes dos santos LuisGuilherme de Oliveira Silva Qesya Rodrigues Ferreira Guilherme Rabelo Mota Heloísa Heim Filipe AntônioFrança da Silva Breno Bittencourt de Brito Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45002175BahiaBrazil Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória da Conquista 45029-094BahiaBrazil

Coronavirus disease-19(COVID-19)has become a pandemic,being a global health concern since December 2019 when the first cases were *** acute respiratory syndrome coronavirus 2,the COVID-19 causal agent,is aβ-coronavirus that has on its surface the spike protein,which helps in its virulence and pathogenicity towards the ***,effective and applicable diagnostic methods to this disease come as an important tool for the management of the *** use of the molecular technique PCR,which allows the detection of the viral RNA through nasopharyngeal swabs,is considered the gold standard test for the diagnosis of ***,serological methods,such as enzyme-linked immunosorbent assays and rapid tests,are able to detect severe acute respiratory syndrome coronavirus 2-specific immunoglobulin A,immunoglobulin M,and immunoglobulin G in positive patients,being important alternative techniques for the diagnostic establishment and epidemiological *** the other hand,reverse transcription loop-mediated isothermal amplification also proved to be a useful diagnostic method for the infection,mainly because it does not require a sophisticated laboratory apparatus and has similar specificity and sensitivity to ***,imaging exams provide findings of typical pneumonia,such as the ground-glass opacity radiological pattern on chest computed tomography scanning,which along with laboratory tests assist in the diagnosis of COVID-19.

关键词： COVID-19 Pandemic Diagnosis Polymerase chain reaction Molecular biology Serology

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COVID-19 liver and gastroenterology findings:An in silico analysis of SARS-CoV-2 interactions with liver molecules

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World Journal of Hepatology 2022年第6期14卷 1131-1141页

作者： Gabrielle Caroline Peiter Cristiano de Bem Torquato de souza Lucca Miketen de Oliveira Luis Gustavo Pagliarin Valentina Nunes Fontoura dos Anjos Filipe Antônio França da Silva Fabrício Freire de Melo Kádima Nayara Teixeira Universidade Federal do Paraná Campus ToledoToledo 85919-899ParanáBrazil Universidade Federal da Bahia Campus Anísio TeixeiraVitória da Conquista 45029-094BahiaBrazil

BACKGROUND Coronavirus disease 19(COVID-19)has not only been shown to affect the respiratory system,but has also demonstrated variable clinical presentations including gastrointestinal tract *** addition,abnormalities in liver enzymes have been reported indicating hepatic *** is known that severe acute respiratory syndrome coronavirus-2(SARS-CoV-2)might infect cells via the viral receptor angiotensin-converting enzyme 2(ACE2)which is expressed in several organs including the *** viral Spike glycoprotein binds to ACE2 and must be cleaved by Furin and Type 2 Serine Protease to enter the *** that,the Akt/mTOR signaling pathway is activated and several COVID-19 changes are *** To analyze liver and gastrointestinal symptoms and cell signaling pathways triggered by SARS-CoV-2 infection due to virus-liver interactions in *** In this in silico study,the three-dimensional structures of the Akt,mTORC1 and Furin(receptors)were selected from the Protein Data Bank(PDB)and the structures of inhibitors(ligands)MK-2206,CC-223 and Naphthofluorescein were selected from PubChem and ZINC *** files were downloaded as 2D structures and converted to optimized 3D structures using ViewerLite 4.2 *** Sketch®software was used to calculate prediction of the protonated form of inhibitors in a physiological environment(pH 7.4).AutoDock Tools(ADT)software was used to calculate and delimit the Grid box used in the molecular docking of each structure selected in the *** addition,protonated ligands were prepared for molecular docking using ADT *** docking was performed using ADT software tools connected to Vina *** of the amino acid residues involved in ligand interactions,as well as ligand twists,the atoms involved in interactions,bond type and strength of interactions were performed using PyMol^(■)and Discovery Studio^(■)(BIOVIA)*** Molecular docking analysis showed that the mTOR

关键词： Bioinformatics Cell signaling pathway COVID-19 Liver injury SARS-CoV-2

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Longitudinal Care Evaluation in Child Healthcare

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Open Journal of Nursing 2016年第10期6卷 902-909页

作者： Dandara Rayssa Silva de souza Tainara Lôrena dos santos Ferreira Fábia Barbosa de Andrade Undergraduate Nursing in the Health Sciences School (FACISA) Federal University of Rio Grande do Norte (UFRN) Santa Cruz/RN Brazil Health Sciences School (FACIS) Federal University of Rio Grande do Norte (UFRN) Santa Cruz/RN Brazil

Objective: To evaluate the essential attribute of Primary Health Care, longitudinal care, care directed at children from birth to two years old. Methods: This is a descriptive and exploratory study of evaluative character and quantitative approach, conducted with parents/caregivers of 186 children, younger than two years old, patients of primary health care services in the city of Santa Cruz/RN, Brazil. For data collection, the instrument Primary Care Assessment Tools (PCA Tools) was used, and the results of the questions dealing with longitudinal attribute were evaluated. The data were stored and processed in Statistical Package for Social Sciences (SPSS). The study was approved by the Research Ethics Committee of the Health Sciences School of Trairí, under number 348896. Results: The mean age of children in months was 8.21;84.4% (n = 157) were assisted by the same doctor/nurse every time;in 81.7% (n = 152) of cases the doctor/nurse know the full medical history of the child;73.7% (n = 137) answered that the professional know their child more as a person than just as someone with a health problem;48.9% (n = 91) stated that the doctor/nurse do not know their family very well;86.6% (n = 161) reported finding the doctor/nurse understands what is saying or questioning;96.2 (n = 179) of respondents said the doctor/nurse answers the questions so that they understand;96.2% (n = 179) of the interviewed said they feel comfortable telling the concerns or problems of their child to the doctor/nurse;66.7% (n = 124) claimed that they would not change the service/doctor/nurse to another health service. Conclusion: It is concluded that the attribute was well rated by the mothers of children seen in primary health care services and that they can establish good communication and relationship with the health professionals who treat their children.

关键词： Primary Health Care Child Health Health Services Evaluation

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Disability progression among leprosy patients released from treatment:a survival analysis

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Infectious Diseases of Poverty 2020年第3期9卷 133-133页

作者： Aleksandra Rosendo dos santos Pãmela Rodrigues de souza Silva Peter Steinmann Eliane Ignotti 不详 Swiss Tropical and Public Health Institute(Swiss TPH) BaselSwitzerland University of Basel BaselSwitzerland

Background:Leprosy can be cured,but physical disability(PD)as a result of the infection can progress in the postrelease from treatment *** study evaluated the likelihood of,and factors associated with,the progression of the PD grade post-release from treatment among leprosy patients treated in Cáceres-MT,Brazil in the period 2000 to ***:A retrospective cohort study and survival analysis were performed in the hyperendemic municipality of Cáceres in the state of Mato *** study population consisted of newly diagnosed leprosy patients released from treatment between January 1,2000 and December 31,*** main outcome was the progression of the PD grade with regard to probability and time;and the evaluated covariates included clinical,operational and demographic *** Cox proportional risk model was used to estimate the risk ratio(Hazard Ratios)of the *** an univariate and a multivariate analysis were implemented,with 95%confidence ***:The mean time for progression of the PD grade was 162 months for PB and 151 months for MB leprosy *** survival curve showed that 15 years after the release from treatment,the probability of PD grade progression was 35%,with no difference between PB and MB or age *** reactions and registered medical complaints of any kind during treatment were identified as risk factors with Hazard Ratios of 1.6 and 1.8 ***:People released from treatment as cured of leprosy are susceptible to worsening of the PD,especially those who have had complications during multi-drug therapy *** indicates that leprosy patients should be periodically monitored,even after the successful completion of multidrug therapy.

关键词： Leprosy Physical disability Cure Progression Survival

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Genome-Wide Identification and Characterization of the DofTranscription Factor Gene Family in Phaseolus vulgarisL.

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American Journal of Plant Sciences 2017年第12期8卷 3233-3257页

作者： Tania Mayumi Ito Claudia Borsari Trevizan Tiago Benedito dos santos Silvia Graciele Hülse de souza Programa de Pós-Graduacao em Biotecnologia Aplicada à Agricultura Laboratório de Biologia Molecular Universidade Paranaense (UNIPAR) Umuarama Brazil Programa de Pós-Graduacao em Agronomia Universidade do Oeste Paulista (UNOESTE) Presidente Prudente Brazil

The Dof (DNA-binding with one finger) proteins are a class of plant-specific transcription factors that can trigger several processes involved in plant growth and development, as well as in stress responses. Here, we performed a systematic bioinformatics analysis to characterize all Dof genes in common bean, which included analysis of the genome sequence, conserved protein domains, chromosomal locations, subcellular locations, phylogenetic relationships, gene duplications, and gene expression profiles in different tissues. Bioinformatics analysis revealed 36 putative genes related to PvDof that were classified into seven subfamilies (A, B1, B2, C1, C2, D1, and, D2) by comparative phylogenetic analysis. Based on our genome duplication analysis, a total of 36 genes were found to be distributed on all 11 chromosomes, and they expanded through gene duplication in tandem, suggesting the involvement of segmental duplication events in the evolutionary process. Synteny events and phylogenetic comparisons of the Dof proteins of common bean with those of A. thaliana, O. sativa, and G. max L. led to the identification of several orthologous and paralogous genes, which provided further insight into the diversity of the evolutionary characteristics of genes of this family in other plant species. Expression profiles revealed that most of the PvDof genes were expressed in different tissues, indicating that PvDof genes may be involved in various physiological functions during plant development. The results of this study provide additional information and potential biotechnological resources for further understanding the molecular basis of this gene family and consequently improvement of common bean crops.

关键词： Common Bean DNA-Binding with One Finger (Dof) Domain Proteins Tran-scription Factor

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Litter decomposition and nutrient release for two tropical N-fixing species in Rio de Janeiro,Brazil

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Journal of Forestry Research 2022年第2期33卷 487-496页

作者： David Pessanha Siqueira Giovanna Campos Mamede Weiss de Carvalho JoséGabriel de souza Silva Marcos Vinicius Winckler Caldeira Deborah Guerra Barroso Laboratório de Fitotecnia Universidade Estadual do Norte Fluminense Darcy RibeiroAvenida Alberto Lamego2000Campos dos GoytacazesRio de JaneiroBrazil Departamento de Ciências Florestais e da Madeira Universidade Federal do Espírito SantoAvenida Governador Lindenberg316Jerônimo MonteiroEspírito SantoBrazil Department of Geosciences and Natural Resources Management University of CopenhagenRolighedsvej 23Frederisksberg CDenmark

Litter production and decomposition are critical to forest productivity,nutrient cycling,and carbon seques-tration in tropical woody ***,nutrient release and leaf litter stoichiometry in tropical legume tree plantations over the long term after outplanting are poorly understood or even *** improving our under-standing of the pattern of changes in the decomposition of N-fixing leaf litters and their possible impact on carbon stor-age,we measured litter production,mass loss and nutrient release for 240 d during litter decomposition for two tropical legume tree species(Plathymenia reticulata and Hymenaea courbaril),in Rio de Janeiro,*** production for *** was 5.689 kg ha^(−1) a^(−1) and 3.231 kg ha^(−1) a^(−1) for *** patterns of mass loss rates were similar;however,nutrient release was greater for ***,while *** showed immobilization of nutrients,especially for N,which increased by almost 20%in the early phase of decomposition followed by gradual *** from the N-fixing species did differ in nutrient chemistries over time,which was not surprising given that initial nutrient concentrations varied broadly,except for C and *** of the nutrient concentrations increased as the remaining litter mass decreased in both species,except for C and *** C:N and N:P ratios differed between the species,but N:P did not correlate to mass *** species had N-rich leaves,but *** decomposition was very likely P-limited,while *** seemed to be co-limited by N and *** results showed different patterns in nutrient release and the stoichi-ometry involved in the decomposition dynamics of the two tropical N-fixing species,even though they have similar litter decay *** species,but especially ***,may help re-establish nutrient cycling in disturbed ecosystems.

关键词： Nutrient dynamics Legume species Litter chemistry Ecological stoichiometry Tropical forest

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