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Neonatal Fc receptor-targeted lignin-encapsulated porous silicon nanoparticles for enhanced cellular interactions and insulin permeation across the intestinal epithelium

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Bioactive Materials 2022年第3期7卷 299-315页

作者： Joao P.Martins Patrícia Figueiredo Shiqi Wang Erika Espo Elena Celi Beatriz Martins Marianna Kemell Karina Moslova Ermei Makil Jarno Salonen Mauri A.Kostiainen Christian Celia Vincenzo Cerullo Tapani Viitala Bruno Sarmento Jouni Hirvonen helder a.santos Drug Research Program Division of Pharmaceutical Chemistry and TechnologyFaculty of PharmacyUniversity of HelsinkiFI-00014HelsinkiFinland Department of Pharmacy University of Chieti-Pescara“G d’Annunzio”I-66100ChietiItaly Drug Research Program Division of Pharmaceutical BiosciencesFaculty of PharmacyUniversity of HelsinkiFI-00014HelsinkiFinland Department of Chemistry University of HelsinkiFI-00014HelsinkiFinland Department of Physics and Astronomy University of TurkuFI-20014TurkuFinland Biohybrid Materials Department of Bioproducts and BiosystemsAalto UniversityFI-00076AaltoFinland i3S-Instituto de Investigaçao e Inovaçao em Saúde University of Porto4200-135PortoPortugal INEB-Instituto de Engenharia Biomedica University of Porto4200-135PortoPortugal CESPU-Instituto de Investigaçao e Formaçao Avançada em Ciencias e Tecnologias da Saúde 4585-116GandraPortugal Helsinki Institute of Life Science(HiLIFE) University of HelsinkiFI-00014HelsinkiFinland

Oral insulin delivery could change the life of millions of diabetic patients as an effective,safe,easy-to-use,and affordable alternative to insulin injections,known by an inherently thwarted patient ***,we designed a multistage nanoparticle(NP)system capable of circumventing the biological barriers that lead to poor drug absorption and bioavailability after oral *** nanosystem consists of an insulin-loaded porous silicon NP encapsulated into a pH-responsive lignin matrix,and surface-functionalized with the Fc fragment of immunoglobulin G,which acts as a targeting ligand for the neonatal Fc receptor(FcRn).The developed NPs presented small size(211±1 nm)and narrow size *** NPs remained intact in stomach and intestinal pH conditions,releasing the drug exclusively at pH 7.4,which mimics blood *** formulation showed to be highly cytocompatible,and surface plasmon resonance studies demonstrated that FcRn-targeted NPs present higher capacity to interact and being internalized by the Caco-2 cells,which express FcRn,as demonstrated by Western ***,in vitro permeability studies showed that Fc-functionalized NPs induced an increase in the amount of insulin that permeated across a Caco-2/HT29-MTX co-culture model,showing apparent permeability coefficients(Papp)of 2.37×10􀀀6 cm/s,over the 1.66×10􀀀6 cm/s observed for their non-functionalized ***,these results demonstrate the potential of these NPs for oral delivery of anti-diabetic drugs.

关键词： Nanoparticles Porous silicon Lignin FcRn Insulin Oral drug delivery

The effect of drying of Piper hispidinervium bydifferent methods and its influence on the yield ofessential oil and safrole

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Information Processing in Agriculture 2023年第1期10卷 28-39页

作者： helder Kiyoshi Miyagawa Alberdan Silva.santos Systematic Investigation in Biotechnology and Molecular Biodiversity Laboratory Federal University of Para´Bele´m 66075-110PABrazil

The Piper hispidinervium leaves and thin stems were dried under laboratory and field *** drying was performed using a shade dryer operating with and without forced convection and an oven dryer operating at 30 and 40*** experiments were conducted using solar dryers with three different covers,i.e.,transparent,black plastic,and palm straw *** essential oil extraction was performed by steam distillation,and the safrole content was analyzed by gas *** mathematical models(Page,logarithmic,Henderson and Pabis,fractional,and diffusion)were fitted with the experimental data and compared based on the coefficient of determination(R2),root mean square error(RMSE)and *** suggest that the best model was the logarithmic model(R2>0.99,RMSE<0.0005,and v2<0.005).With sufficient drying,the safrole content increased up to 95%of the extracted oil;however,when the drying time was prolonged,both the oil yield and safrole content of the extracted oil decreased.

关键词： Modeling Drying kinetics Essential oil extraction Safrole extraction Piperaceae

A prospective cancer chemo-immunotherapy approach mediated by synergistic CD326 targeted porous silicon nanovectors

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Nano Research 2015年第5期8卷 1505-1521页

作者： Mohammad-Ali Shahbazi Neha Shrestha Ermei Makila Francisca Araujo Alexandra Correia Tomas Ramos Bruno Sarmento Jarno Salonen Jouni Hirvonen helder A. santos Division of Pharmaceutical Chemistry and Technology Faculty of Pharmacy University ofHelsinki FI-00014 Helsinki Finland Laboratory of Industrial Physics Departmen t of Physics and Astronomy University of Turku FI-20014 Turku Finland INEB - Instituto de Engenharia Biomedica NewTherapies Group Universidade do Porto Rua do Campo Alegre 823 4150-180 Porto Portuga ICBAS - Instituto Ciencias Biomedicas Abel Salazar University of Porto Rua de Jorge Viterbo Ferreira 4050-313 Porto Portugal Faculty of Pharmacy University of Coimbra 3000-548 Coimbra Portugal IINFACTS - Instituto de Investigacao e Formacao Avancada em Ciencias e Tecnologias da Saude Instituto Superior de Ciencias da Saude-Norte Department of Pharmaceutical Sciences CESPU Rua Central de Gandra 4585-116 Gandra Portugal

Combination therapy via nanoparticulate systems has already been proposed as a synergistic approach for cancer treatment. Herein, undecylenic acid modified thermally hydrocarbonized porous silicon nanoparticles （UnTHCPSi NPs） loaded with sorafenib and surface-biofunctionalized with anti-CD326 antibody （Ab） were developed for cancer chemo-immunotherapy in MCF-7 and MDA-MB-231 breast cancer cells. The cytocompatibility study showed no significant toxicity for the bare and antibody-conjugated UnTHCPSi （Un-Ab） NPs at concentrations lower than 200 μg·mL^-1. Compared to the bare UnTHCPSi, Un-Ab NPs loaded with sorafenib reduced the premature drug release in plasma, increasing the probability of proper drug targeting. In addition, high cellular interaction and subsequent internalization of the Un-Ab NPs into the cells expressing CD326 antigen demonstrated the possibility of improving antigen-mediated endocytosis via CD326 targeting. While an in vitro antitumor study revealed a higher inhibitory effect of the sorafenib-loaded Un-Ab NPs compared to the drug-loaded UnTHCPSi NPs in the CD326 positive MCF-7 cells, there was no difference in the anti-proliferation impact of both the abovementioned NPs in the CD326 negative MDA-MB-231 cells, suggesting CD326 as an appropriate receptor for Ab-mediated drug delivery. It was also shown that the anti-CD326 Ab can act as an immunotherapeutic agent by inducing antibody dependent cellular cytotoxicity and enhancing the interaction of effector immune and cancer cells for subsequent phagocytosis and cytokine secretion. Hence, the developed nanovectors can be applied for simultaneous tumor-selective drug targeting and immunotherapy.

关键词： CD326 antibody porous silicon nanopartides chemo-immunotherapy breast cancer drug targeting

The full length <i>PtSRP</i>(<i>Pisolithus tinctorius</i>symbiosis related protein) fungal mRNA encodes a potential marker of ectomycorrhiza formation

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American Journal of Molecular Biology 2012年第3期2卷 258-264页

作者： helder Elísio E. Vieira Cláudia E. P. Lima Carlos E. Calzavara-Silva Bartolomeu Acioli-santos Elaine Malosso Departamento de Micologia Universidade Federal de Pernambuco Recife Brazil Laboratório de Imunologia Celular e Molecular Centro de Pesquisas René Rachou Belo Horizonte Brazil

The Pisolithus tinctorius symbiosis related protein expressed sequence tag (EST PtSRP) was previously identified in the first hours of the interaction between the fungus Pisolithus tinctorius and sweet chestnut Castanea sativa, and partially characterized as a fungal marker gene of ectomycorrhizal symbiosis formation. We used the 5’ rapid amplification of cDNA ends (RACE) to obtain the PtSRP mRNA 5’ region, and together with our previously reported 3’ mRNA region, the full mRNA sequence was assembled by use of bioinformatics tools and deposited to GenBank (Accession: GU733439). The full-length mRNA sequence (636 bp) revealed the locations of the 5’ and 3’ untranslated regions (UTRs) and contained the Kozak sequence (ccc aag ATG A) in the 5’ UTR. The in silico translated PtSRP open reading frame (ORF) codes for a 127 amino acid protein and contained four putative post-translational modification sites (two N-glycosylation and two phosphorylation). The protein secondary structure is postulated to be composed of one N-terminal hydrophobic transmembrane alpha helix and at least six hydrophilic beta-strands spread across the protein. Sub-cellular localization prediction suggests that the protein is involved in cellular secretory pathway, supported by the presence of a cleavage site motif close to the membrane anchor. The data presented herein indicate the role of PtSRP as a fungal membrane secreted protein involved in early stages of ectomycorrhizal formation, with application as a possible marker for nascent ectomy-corrhiza fungal development.

关键词： Ectomycorrhiza Symbiosis Related Genes/Proteins RACE EST

彩云之南,雨林天堂——西双版纳万达文华度假酒店

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城市环境设计 2016年第5期 226-229页

作者：李颖悟 helder santos 陈江陈英男陈鹤 OAD欧安地建筑设计事务所

西双版纳,古代傣语为"勐巴拉娜西",是"理想而神奇的乐土"之意。西双版纳万达文华度假酒店坐落于中国云南省最南端,西双版纳景洪市嘎栋区东北方向,群山密林的优美风景中,建筑融于自然,也成为了风景的一部分。建筑师充分发掘了西双版纳热... 详细信息

西双版纳,古代傣语为"勐巴拉娜西",是"理想而神奇的乐土"之意。西双版纳万达文华度假酒店坐落于中国云南省最南端,西双版纳景洪市嘎栋区东北方向,群山密林的优美风景中,建筑融于自然,也成为了风景的一部分。建筑师充分发掘了西双版纳热带自然环境和少数民族风情文化的独特资源,融入傣族建筑的整体风格,让建筑在内外空间上的关照与交流延伸至精神的层面,呈现出了从属于自然环境的气质,提供了一个隐于自然风景、私密幽静的度假乐土。

关键词：度假酒店达文文华酒店西双版纳云南雨林彩云之南天堂

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花水湾名人度假酒店

中国建筑装饰装修 2011年第2期 80-85页

作者：李颖悟何樾温凡松 helder santos Irwin 董谦魏岢苏欣彭敏杰吕婧婧美国OAD建筑设计事务所成都市建筑设计院

精选理由借问酒家何处有,牧童遥指杏花村＂大自然的魅力结合古人诗词意境,轻柔的出现在空间里。一切人工设计都是遵照自然的感觉,力求与环境融为一体,故建筑室内外装饰均以＂花、水＂为主题进行设计,尽显山海中的＂花与水＂之感。

关键词：度假酒店名人水人工设计室内外装饰大自然酒家空间