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Separate basolateral and apical phosphatidylcholine secretion routes in intestinally differentiated tumor cells

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World Journal of Gastroenterology 2009年第46期15卷 5821-5826页

作者： Daniel Gotthardt Annika Braun Anke Tietje Karl heinz Weiss Robert Ehehalt Wolfgang R Stremmel Department of Gastroenterology University Hospital Heidelberg

AIM:To investigate whether the secretion of phospha-tidylcholine(PC)in intestinal mucus occurs by apical secretion or via basolateral excretion and to determine its subsequent passage across the tight junctions to the apical ***:We addressed this question using the po-larized intestinally differentiated tumor cell line CaCo-2 grown on filters to confluence in Transwell culture *** released PC and sphingomyelin(Sph)from apical and basolateral media were analyzed by mass ***:The secreted PC species were identical in both compartments indicating the same intracellular origin of ***,PC secretion into the basolateral compart-ment was more effective,and the PC:Sph ratio in the ba-solateral compartment was signif icantly higher than that in the apical compartment(8.18 ± 1.84 vs 4.31 ± 1.22,P = 0.01).Both pathways were temperature sensitive and were unaltered in the presence of ***:The data demonstrate the PC secre-tion capacity of CaCo-2 cells and indicate two sepa-rated apical and basolateral release mechanisms.

关键词： CaCo-2 cells Epithelial cells Mass spectrometry Phosphatidylcholine Secretion Sphingomyelin

Predictive value of Ki67 and p53 in locally advanced rectal cancer:Correlation with thymidylate synthase and histopathological tumor regression after neoadjuvant 5-FU-based chemoradiotherapy

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World Journal of Gastroenterology 2008年第7期14卷 1060-1066页

作者： Christiane Jakob Torsten Liersch Wolfdietrich Meyer heinz Becker Gustavo B Baretton Daniela E Aust Institute for Pathology University of TechnologyFetscherstr. 74D-01307 DresdenGermany Department of General Visceral and Transplantation SurgeryUniversity Medical Center of GttingenRobert-Koch-Str. 40D-37075 GttingenGermany

AIM: To investigate the predictive value of Ki67 and p53 and their correlation with thymidylate synthase (TS) gene expression in a rectal cancer patient cohort treated according to a standardized recommended neoadjuvant treatment ***: Formalin fixed, paraffin embedded pre-therapeutical tumor biopsies (n = 22) and post-therapeutical resection specimens (n = 40) from patients with rectal adenocarcinoma (clinical UICC stage Ⅱ/Ⅲ) receiving standardized neoadjuvant 5-fluorouracil (5-FU) based chemoradiotherapy were studied for Ki67 and p53 expression by immunohistochemistry and correlated with TS mRNA expression by quantitative TaqMan real-time PCR after laser microdissection. The results were compared with histopathological tumor regression according to a standardized semiquantitative score grading ***: Responders (patients with high tumor regression) showed a significantly lower Ki67 expression than non-responders in the pre-therapeutical tumor biopsies (81.2% vs 16.7%; P < 0.05) as well as in the post-therapeutical resection specimens (75.8% vs 14.3%; P < 0.01). High TS mRNA expression was significantly correlated with a high Ki67 index and low TS mRNA expression was significantly correlated with a low Ki67 index in the pre-therapeutical tumor biopsies (corr. coef. = 0.46; P < 0.01) as well as in the post-therapeutical resection specimens (corr. coef. = 0.40; P < 0.05). No significant association was found between p53 and TS mRNA expression or tumor ***: Ki67 has, like TS, predictive value in rectal cancer patients after neoadjuvant 5-FU based chemoradiotherapy. The close correlation between Ki67 and TS indicates that TS is involved in active cell cycle processes.

关键词： p53 Ki67 Neoadjuvant treatment Rectal cancer Thymidylate synthase

Parkinson’s disease and translational research

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Translational Neurodegeneration 2020年第4期9卷 547-557页

作者： Elisabeth Dinter Theodora Saridaki Leonie Diederichs heinz Reichmann Bjorn H.Falkenburger Department of Neurology Technische Universitat DresdenDresdenGermany Deutsches Zentrum fur Neurodegenerative Erkrankungen DresdenGermany Department of Neurology RWTH University AachenAachenGermany

Parkinson's disease(PD)is diagnosed when patients exhibit bradykinesia with tremor and/or rigidity,and when these symptoms respond to dopaminergic *** in the last years there was a greater recognition of additional aspects of the disease including non-motor symptoms and prodromal states with associated pathology in various regions of the nervous *** this review we discuss current concepts of two major alterations found during the course of the disease:cytoplasmic aggregates of the protein a-synuclein and the degeneration of dopaminergic *** provide an overview of new approaches in this field based on current concepts and latest *** many areas,translational research on PD has advanced the understanding of the disease but there is still a need for more effective therapeutic options based on the insights into the basic biological phenomena.

关键词： α-Synuclein Pre-formed fibrils Protein aggregates Aggresome Dopamine deficiency Medium spiny neurons Autophagy

Patients without hepatocellular carcinoma progression after transarterial chemoembolization benefit from liver transplantation

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World Journal of Gastroenterology 2007年第5期13卷 761-767页

作者： Aiman Obed Alexander Beham Kerstin Püllmann heinz Becker Hans J Schlitt Thomas Lorf Department of Surgery University of Regensburg Regensburg 93053Germany 不详 Department of Surgery University of Goettingen Goettingen 37073 Germany

AIM： To assess the outcome of patients, who underwent transarterial chemoembolization （TACE） for hepatocellular carcinoma （HCC） and subsequently liver transplantation （OLT） irrespective of tumor size when no tumor progression was observed. METHODS： Records, imaging studies and pathology of 84 patients with HCC were reviewed. Ten patients were not treated at all, 67 patients had TACE and 35 of them were listed for OLT. Tumor progression was monitored by ultrasound and AFP level every 6 wk. Fifteen patients showed signs of tumor progression without transplantation. The remaining 20 patients underwent OLT. Further records of 7 patients with HCC seen in histological examination after OLT were included. RESULTS： The patients after TACE without tumor progression underwent transplantation and had a median survival of 92.3 too. Patients, who did not qualify for liver transplantation or had signs of tumor progression had a median survival of 8.4 mo. The patients without treatment had a median survival of 3.8 mo. Independent of International Union Against Cancer （UICC） stages, the patients without tumor progression and subsequent OLT had longer median survival. No significant difference was seen in the OLT treated patients if they did not fulfill the Milan criteria. CONCLUSION： Selection of patients for OLT based on tumor progression results in good survival. The evaluation of HCC patients should not only be based on tumor size and number of foci but also on tumor progression and growth behavior under therapy.

关键词： Liver transplantation Hepatocellularcarcinoma

Copper toxicosis gene MURRl is not changed in Wilson disease patients with normal blood ceruloplasmin levels

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World Journal of Gastroenterology 2006年第14期12卷 2239-2242页

作者： Karl heinz Weiss Uta Merle Mark Schaefer Peter Ferenci Joachim Fullekrug Wolfgang Stremmel Department of Gastroenterology University of Heidelberg Germany Department of Gastroenterology and Hepatology Medical University of Vienna Austria

AIM： To analyze our Wilson disease patient cohort （n = 106） for alterations in the gene coding for MURR1. METHODS： Patients with an established diagnosis of Wilson disease but normal ceruloplasmin blood levels were chosen for our study （n = 14）. Patients with two known disease-causing mutations in the ATPTB gene were not included. The three exons of the human MURR1 gene were sequenced after amplification of the genomic DNA by polymerase chain reaction. RESULTS： Our study did not reveal any mutations leading to an amino acid change in the MURR1 sequence of Wilson disease patients. A polymorphism at 472 bp of the coding sequence could be confirmed. CONCLUSION： The MURRI gene plays no role in the pathogenesis of Wilson disease patients with normal serum ceruloplasmin levels.

关键词： Wilson Disease ATPTB MURR1 COMMD1

Significance of Caucasian Sections for Working out Carbon-Isotope Standard for Upper Permian and Lower Triassic (Induan) and Their Correlation with the Permian of North-Eastern Russia

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Journal of China University of Geosciences 2005年第2期16卷 141-151页

作者： Yuri D Zakharov Alexander S Biakov Aymon Baud heinz Kozur Far Eastern Geological Institute Russian Academy of Sciences (Far Eastern Branch) Vladivostok 690022 Russia North-East Interdisciplinary Scientific Research InstituteRussian Academy of Sciences (Far Eastern Branch)Magadan 685000 RussiaMusee de GeologieBFSH2-UNI 1015 Lausanne Switzerland Rézsü u. 83H-1029 Budapest Hungary

Data obtained on conodont distribution in the Permian-Triassic Sovetashen Section of Transcaucasia provide further limitations on the age of the carbon-isotopic anomalies discovered by Baud et al. (1989). The significance of Caucasian sections for working out the carbon-isotope standard for the Upper Permian and Lower Triassic (Induan) is shown. Original data on carbon-isotope composition of bivalve and brachiopod shells from Permian sediments of North-Eastern Russia (Omolon and Okhotsk areas) have been obtained, which may be used for their correlation.

关键词： Armenia North-Eastern Russia Permian Triassic conodonts ammonoids bivalves brachiopods isotopic composition paleotemperatures correlation

Shear strength behavior of geotextile/geomembrane interfaces

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Journal of Rock Mechanics and Geotechnical Engineering 2015年第6期7卷 638-645页

作者： Belén M.Bacas Jorge Caizal heinz Konietzky Terrasolum S.L.Geotechnical Engineering Technology Development Center of University of Cantabria(CDTUC) School of Civil Engineering University of Cantabria Geotechnical Institute TU Bergakademie Freiberg

This paper aims to study the shear interaction mechanism of one of the critical geosynthetic interfaces,the geotextile/geomembrane, typically used for lined containment facilities such as landfills. A largedirect shear machine is used to carry out 90 geosynthetic interface tests. The test results show a strainsoftening behavior with a very small dilatancy （〈0.5 mm） and nonlinear failure envelopes at a normalstress range of 25e450 kPa. The influences of the micro-level structure of these geosynthetics on themacro-level interface shear behavior are discussed in detail. This study has generated several practicalrecommendations to help professionals to choose what materials are more adequate. From the threegeotextiles tested, the thermally bonded monofilament exhibits the best interface shear strength underhigh normal stress. For low normal stress, however, needle-punched monofilaments are *** the regular textured geomembranes tested, the space between the asperities is an important *** closer these asperities are, the better the result achieves. For the irregular textured geomembranestested, the nonwoven geotextiles made of monofilaments produce the largest interface shear strength.

关键词： Geotextiles Geomembranes Landfills Fiber length Roughness Shear strength Friction angle

Vascularization mediated by mesenchymalstem cells from bone marrow and adiposetissue: a comparison

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Cell Regeneration 2015年第1期4卷 106-115页

作者： Karoline Pill Sandra Hofmann heinz Redl Wolfgang Holnthoner Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Donaueschingenstrasse 131200ViennaAustria Austrian Cluster for Tissue Regeneration ViennaAustria Department of Biomedical Engineering Eindhoven University of Technology5600 MB EindhovenThe Netherlands Institute for Complex Molecular Systems Eindhoven University of TechnologyPO Box 5135600 MB EindhovenThe Netherlands Institute for Biomechanics Swiss Federal Institute of Technology ZürichVladimir-Prelog-Weg 38093 ZürichSwitzerland

Tissue-engineered constructs are promising to overcome shortage of organ donors and to reconstruct at least partsof injured or diseased tissues or organs. However, oxygen and nutrient supply are limiting factors in many tissues,especially after implantation into the host. Therefore, the development of a vascular system prior to implantationappears crucial. To develop a functional vascular system, different cell types that interact with each other need tobe co-cultured to simulate a physiological environment in vitro. This review provides an overview and a comparison ofthe current knowledge of co-cultures of human endothelial cells (ECs) with human adipose tissue-derived stem/stromal cells (ASCs) or bone marrow-mesenchymal stem cells (BMSCs) in three dimensional (3D) hydrogel *** stem cells (MSCs), BMSCs or ASCs, have been shown to enhance vascular tube formation of ECs and toprovide a stabilizing function in addition to growth factor delivery and permeability control for ECs. Althoughphenotypically similar, MSCs from different tissues promote tubulogenesis through distinct mechanisms. In this report,we describe differences and similarities regarding molecular interactions in order to investigate which of these two celltypes displays more favorable characteristics to be used in clinical applications. Our comparative study shows that ASCsas well as BMSCs are both promising cell types to induce vascularization with ECs in vitro and consequently arepromising candidates to support in vivo vascularization.

关键词： Vascularization Endothelial cells Mesenchymal stem cells Co-culture Molecular mechanisms

Novel Design Solutions for Fishing Reel Mechanisms

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Chinese Journal of Mechanical Engineering 2015年第4期28卷 726-736页

作者： LOVASZ Erwin-Christian MODLER Karl-heinz NEUMANN Rudolf GRUESCU Corina Mihaela PERJU Dan CIUPE Valentin MANIU Inocentiu Department of Mechatronics University Politehnica Timisoara Institut für Festkrpermechanik Technische Universitt Dresden

Currently, there are various reels on the market regarding the type of mechanism, which achieves the winding and unwinding of the line. The designers have the purpose of obtaining a linear transmission function, by means of a simple and small-sized mechanism. However, the present solutions are not satisfactory because of large deviations from linearity of the transmission function and complexity of mechanical schema. A novel solution for the reel spool mechanism is proposed. Its kinematic schema and synthesis method are described. The kinematic schema of the chosen mechanism is based on a noncircular gear in series with a scotch-yoke mechanism. The yoke is driven by a stud fixed on the driving noncircular gear. The drawbacks of other models regarding the effects occurring at the ends of the spool are eliminated through achieving an appropriate transmission function of the spool. The linear function approximation with curved end-arches appropriately computed to ensure mathematical continuity is very good. The experimental results on the mechanism model validate the theoretical approach. The developed mechanism solution is recorded under a reel spool mechanism patent.

关键词： reel mechanism noncircular gears analysis synthesis transmission function