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Image cytometric DNA analysis of mucosal biopsies in patients with primary achalasia

World journal of Gastroenterology 2006年第19期12卷 3020-3025页

作者： I Gockel P K(a|¨)mmerer j Brieger UR heinrich Wj Mann F Bittinger VF Eckardt T junginger Department of General and Abdominal Surgery johannes Gutenberg-University Mainz Laboratory of Tumor Biology Department of Otolaryngology Johannes Gutenberg-UniversityMainz Institute of Pathology johannes Gutenberg-University Mainz Department of Gastroenterology German Diagnostic Clinic Wiesbaden

AIM： To determine DNA aneuploidy in mucosal biopsies of achalasia patients for subsequent rapid diagnosis. METHODS： Biopsies from the middle third of the esophagus were obtained in 15 patients with achalasia. Immunohistochemical staining was carried out with monoclonal antibodies MIB-1 for Ki67 and PAb 1801 for p53, in addition to the conventional histologic examination for dysplasia. Nuclei of fresh biopsy material were enzymatically and mechanically isolated, and the DNA content was determined with image cytometry after Feulgen staining. DNA grading of malignancy was assessed according to Boecking to determine the variability of DNA values noted around the normal diploid peak. Further indices measured included the aneuploid rate, and the 5c-, 7c- and 9c-exceeding rate. RESULTS： The histological examination did not demonstrate dysplasia; while MIB-1 （basal） showed a positive reaction in 8/15 achalasia specimens, p53 was negative in all specimens. Image cytometric DNA analysis detected aneuploidy in 4/15 （26.7%） specimens. Samples from 15 patients with squamous cell carcinoma as well as specimens obtained exclusively 2 cm proximal to the tumor served as reference tests. All carcinomas （15/15） as well as 9 of the peritumoral samples （9/15） were aneuploid. The comparison of biopsies from achalasia patients with peritumoral and carcinoma specimens revealed statistically significant differences regarding the aneuploid rate （diploid： P 〈 0.0001; tetraploid： P = 0.001）, grading of malignancy according to Boecking （P 〈 0.0001） and the 5c- （P 〈 0.0001）, 7c-（P 〈 0.0001）, and 9c- （P = 0.0001） exceeding rate with progredient DNA alterations in the respective order. CONCLUSION： The finding that DNA aneuploidy was identified by image cytometry in esophageal specimens of patients with achalasia, which may be due to specific chromosomal alterations presenting as precancerous lesions in 27% of patients, leads us to conclude that image cytometry represents a valuable screening

关键词： Achalasia Precancerous epithelial alterations Esophageal carcinoma Image cytometric DNA analysis Proliferation marker Ki67 Tumor suppressor gene p53

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基于扫描隧道显微镜的单原子自旋共振技术

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真空科学与技术学报 2021年第10期41卷 897-912页

作者：杨锴 Andreas heinrich Christopher Lutz 中国科学院物理研究所北京100190 韩国基础科学研究院量子纳米科学中心首尔03760 韩国梨花女子大学物理系首尔03760 美国IBM Almaden研究中心圣何塞95120

近年来,应用扫描隧道显微镜技术已经可以测量单个原子的电子自旋共振谱线,为实现原子尺度量子磁性的探测与操控迈出了重要一步。电子自旋共振扫描隧道显微镜具有原子分辨能力和几十个纳电子伏的超高能量分辨率,可以实现微弱信号的原子... 详细信息

近年来,应用扫描隧道显微镜技术已经可以测量单个原子的电子自旋共振谱线,为实现原子尺度量子磁性的探测与操控迈出了重要一步。电子自旋共振扫描隧道显微镜具有原子分辨能力和几十个纳电子伏的超高能量分辨率,可以实现微弱信号的原子尺度探测,例如可以测量固体表面相距几纳米的两个原子之间的微弱磁偶极相互作用、单个原子的电子与核自旋之间的超精细相互作用,以及人工自旋阵列的量子涨落等。借助脉冲式电子自旋共振技术,可以进一步实现固体表面单个磁性原子以及耦合原子的量子相干操控,测量其拉比振荡、拉姆齐干涉条纹和自旋回波信号等。单原子脉冲式电子自旋共振的实现为应用单原子量子探针进行量子探测奠定了重要基础。另外,对具有原子级精度的人工自旋结构的量子相干操控,为多体系统的量子模拟提供了重要的固态实验平台。

关键词：扫描隧道显微镜电子自旋共振磁性原子量子探测量子模拟

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Particle formulation of hydroalcoholic rosemary (Rosmarinus officinalis L.) extracts using a spouted bed

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Particuology 2020年第4期18卷 26-34页

作者： D.Peshev E.Eichner M.Goslinska S.Pietsch Y.Trambabova T.Terzieva N.Georgieva S.heinrich Department of Chemical Engineering University of Chemical Technology and Metallurgy1576 SofiaBulgaria Institute of Solids Process Engineering and Particle Technology Hamburg University of Technology21073 HamburgGermany Department of Biotechnology University of Chemical Technology and Metallurgy1576 SofiaBulgaria

In this study,spouted bed technology was applied to the protection and functionalization of polyphenolic rosemary ***,solid-liquid extraction was performed at 25℃with *** ethanol-water ratio was chosen to obtain high phenolic acid and diterpene concentrations in the *** selected solvent composition gave liquid extracts that could be directly added to a coating mixture for feeding into a spouted bed ***,no preliminary solvent exchange or evaporation of the extraction solvent to dryness was *** grade Paselli MD 6 maltodextrin and HiCap 100 modified starch were used as inert core and shell materials in the spouted bed encapsulation process under mild operating *** antioxidant activity of the fresh extract was retained during processing,which indicated that the active constituents were preserved and quantitatively transferred into the encapsulated formulations,which were readily soluble in *** obtained powders were composed of nearly spherical *** compositions,water solubilities,and particle morphologies make them a strong candidate for food and beverage applications as natural antioxidants,preservatives,or functional ingredients.

关键词： Particle Polyphenolic rosemary extract Encapsulation Spouted bed Bioactive Antioxidant

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发作性夜间血红蛋白尿患者出现脑缺血性梗死:2例病例报道和对7例以前报道患者的最新回顾

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世界核心医学期刊文摘（神经病学分册） 2006年第3期2卷 10-11页

作者： audebert H. Planck j. Eisenburg M. 江山 Dept. of Neurology Stdtisches Krankenhaus München-Harlaching Sanatoriumsplatz2 81545 Münc hen Harlaching Germany

Background: Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired clonal stem cell disorder, leading to a deficient biosynthesis of surface proteins in hematopoetic cells. Clinical symptoms consist of various combinations of intravascular hemolysis, bone marrow failure and mainly venous thrombotic events. Arterial thrombosis has been described only in a few cases. Methods: We report two patients with fatal ischemic strokes in PNH caused by arterial occlusion. In addition we also reviewed 7 previously reported cases in the literature and obtained follow-up information on 4 of the 5 survivors. Results: Both patients from our clinic had experienced hemoglobinuria and cytopenia prior to the vascular events but in one case diagnosis of PNH was only established after stroke onset. The causal role of PNH was supported by the absence of other vascular risk factors and by simultaneous symptoms of hemolysis and cytopenia. The diagnosis of PNH was confirmed by a positive result in flow cytometry. Despite extended therapeutic interventions, including antithrombotic treatment (with heparin or tirofiban) and decompressive hemicraniectomy both patients developed further thrombotic complications. The two patients died during their hospital stay from vascular or infectious complications. A review of all 9 published cases revealed that four patients died within a few weeks after the initial thrombotic event. No difference in treatment between patients with good or bad outcome was seen. Conclusions: PNH is a rare cause of arterial stroke but it should be considered in young stroke patients with abnormal blood findings. As yet, there is no effective antithrombotic treatment.

关键词：缺血性梗死血红蛋白尿缺血性卒中感染并发症血管内溶血动脉阻塞骨髓衰竭抗血栓治疗血管危险

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《Room Acoustics》

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声学学报 2017年第4期42卷 F0003-F0003页

作者： heinrich Kuttruff

CRC Press,Talor&Francis Group,ISBN:978-1-4822-6043-4(Hardback),2017,6th *** book represents a classical text in room acoustics-its original edition having been first pubfished in *** author was a chair professor at the Institute of Technical Acoustics,the Technical University in Aachenl Germany since 1972 until his retirement in *** author of the book intends to introduce

关键词：期刊编辑工作书籍推介《Room Acoustics》

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‘Food and medicine continuum’–Why we should promote cross-cultural communication between the global East and West

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Chinese Herbal Medicines 2022年第1期14卷 3-4页

作者： Michael heinrich Ruyu Yao Peigen Xiao Research Group 'Pharmacognosy and Phytotherapy' UCL School of PharmacyUniversity College London CHM Institute of Medicinal Plant Development Chinese Academy of Medical Sciences and Peking Union Medical College

It is estimated that by 2030 one sixth of the global population will be aged over 60(WHO,2021).Aging leads to functional decline,which triggers many chronic diseases and *** is reported that the elderly often suffer from geriatric syndromes such as frailty,sarcopenia,weight loss and dementia(Sanford et al.,2020);for those aged over 85,conditions of hearing and vision loss,decline in immune function,cardiovascular diseases,osteoporosis and dementia are in high prevalence(jaul&Barron,2017).Therefore,aging has been an important risk for chronic diseases and geriatrics globally.

关键词： diseases cardiovascular

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金属标准物质中杂质的辉光放电质谱法溯源分析

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冶金分析 2012年第2期32卷 6-10页

作者： RECKNAGEL Sebastian KIPPHARDT heinrich LANGE Britta HOPPE Marion 德联邦材料测试研究院

标准物质定值分析的溯源性要求比常规分析高很多。在常规分析中,有证标准物质(CRM)可用于校准以及建立可溯源性。尽管与标准物质生产和认定相关的ISO导则并没有十分明确地提到校准,但众多标准物质生产者在定值分析时却不接受基准标准物... 详细信息

标准物质定值分析的溯源性要求比常规分析高很多。在常规分析中,有证标准物质(CRM)可用于校准以及建立可溯源性。尽管与标准物质生产和认定相关的ISO导则并没有十分明确地提到校准,但众多标准物质生产者在定值分析时却不接受基准标准物质用于校准。这些要求制约了标准物质定值方法的使用,因为这些方法可以采用已知高纯度的标准物质、化学计量法及已知高纯度标准物质的混合物进行校准。有效的固体取样技术,例如通常使用的块状标准物质进行校准的辉光放电质谱法(GD-MS)或火花源发射光谱法(spark-OES),似乎不太适合定值分析。尤其是固体样品中痕量元素分析测定的有力工具辉光放电质谱法,除金属杂质外,使用特定气体混合物,这种方法也可以分析相关的非金属杂质如硫和磷。我们开发了一种可以使用已知高纯度的标准物质混合物和化学计量法的校准方法,这种校准方法同样可用于辉光放电质谱法。与溶液中基体匹配技术类似,此校准方法基于掺杂的粉末压片。本文还结合铜和钢标准物质的定值分析结果进行了陈述。

关键词：标准物质溯源性辉光放电质谱法铜钢铁

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CCD器件空间滤波在微粒运动速度检测上的应用

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仪器仪表学报 2000年第2期21卷 211-214页

作者：董正超 Klaus Michel Swen Bergeler Klaus Christofori heinrich Krambeer 山东矿业学院济南分院济南250031 Institutf.AllgemeineElektrotechnik ASTECH GmbH

本文报道了基于空间滤波法对变加速运动物体表面运动速度进行实时测量的实验研究。采用 CCD线阵同时作为空间滤波器和光电转换器件。由 CCD线阵实现的差分栅式空间滤波器不仅消除了零频分量对低速运动测量的不利影响 ,而且导致了一种快... 详细信息

本文报道了基于空间滤波法对变加速运动物体表面运动速度进行实时测量的实验研究。采用 CCD线阵同时作为空间滤波器和光电转换器件。由 CCD线阵实现的差分栅式空间滤波器不仅消除了零频分量对低速运动测量的不利影响 ,而且导致了一种快速动态累加采样方法。实验结果表明 ,这一方法可对变加速物体运动速度进行实时测量。

关键词： CCD线矩空间滤波器光栅运动速度检测

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Post-compression of high-energy,sub-picosecond laser pulses

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High Power Laser Science and Engineering 2023年第2期11卷 117-126页

作者： P.-G.Bleotu j.Wheeler S.Yu.Mironov V.Ginzburg M.Masruri A.Naziru R.Secareanu D.Ursescu F.Perez j.De Sousa D.Badarau E.Veuillot P.audebert E.Khazanov G.Mourou LULI-CNRS CEAUniversite SorbonneEcole PolytechniqueInstitut Polytechnique de ParisPalaiseau CEDEXFrance Doctoral School of Physics University of BucharestBucharest-MagureleRomania Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering(IFIN-HH) MagureleRomania IZEST Ecole PolytechniqueInstitut Polytechnique de ParisPalaiseau CEDEXFrance Independent Researcher Bourg-La-ReineFrance Federal Research Center Institute of Applied Physics of the Russian Academy of Sciences(IAP RAS) Nizhny NovgorodRussia

The post-compression technique based on self-phase modulation of high-energy pulses leads to an increase in achievable peak power and ***,the pulses considered in experiments have been less than 100 fs in ***,the method is applied to the ELFIE laser system at the LULI facility,for a pulse of 7 j energy and an initial measured duration of 350 fs.A 5-mm-thick fused silica window and a 2 mm cyclic-olefin polymer were used as optical nonlinear *** 9 cm diameter beam was spectrally broadened to a bandwidth corresponding to 124 fs Fourier-limited pulse duration,and then it was partly post-compressed to 200 *** measuring the spatial spectra of the beam fluence,a uniform gain factor of 4 increase in the fluctuations over the studied range of frequencies is observed,due to small-scale self-focusing.

关键词： high-power laser nonlinear pulse interaction post-compression self-phase modulation

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HYL—Ⅲ直接还原技术的现状与发展

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烧结球团 1991年第1期16卷 30-34页

作者： Peter heinrich 刘树立

由联邦德国奥伯豪森的美最时公司(MAN GHH AG)、蒂森公司的富乐斯多公司(Ferrostaal AG)和墨西哥蒙特雷的希尔萨公司(HYL S. A)组成的跨国公司所建造的年产2Mt海绵铁的直接还原厂,是建在希尔萨公司之外的第一家自HYL—Ⅲ问世以来的工业... 详细信息

由联邦德国奥伯豪森的美最时公司(MAN GHH AG)、蒂森公司的富乐斯多公司(Ferrostaal AG)和墨西哥蒙特雷的希尔萨公司(HYL S. A)组成的跨国公司所建造的年产2Mt海绵铁的直接还原厂,是建在希尔萨公司之外的第一家自HYL—Ⅲ问世以来的工业性工厂。本文将叙述该厂的配置情况和操作结果.该厂于1989年2月成功地通过了1号机组的运转试验。本文还详细叙述了HYL—Ⅲ工艺中应用CO_2吸收装置的好处并概略地展示了其发展潜力。

关键词：海绵铁直接还原 HYL-Ⅲ工艺

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