检索结果-南通市图书馆

Antibodies as Tailor-Made Chiral Selectors: an Interdisciplinary Approach to enantiomer Separation and Detection

在线全文

学校读者我要写书评

暂无评论

色谱 2005年第5期23卷 508-519页

作者： HOFSTeTTeR heike HOFSTeTTeR Oliver Department of Chemistry and Biochemistry Northern Illinois University DeKalb Illinois 60115 USADepartment of Chemistry and Biochemistry Northern Illinois University DeKalb Illinois 60115 USA

It has long been known that the configurational isomers of biologically active compounds, e.g., nutrients, pesticides, and drugs, may exhibit different activities in a chiral environment such as the human body. Although the majority of drugs presently in development are chiral, analytical and preparative methods for the quantitative determination and purification of stereoisomers still lag behind. One reason is that commonly used chiral selectors for the direct resolution of enantiomers are not tailor-made for a specific analyte. The identification of suitable selectors for a particular pair of enantiomers still requires considerable experimentation and is generally demanding with regard to material, time and labor. The rational design of chiral host molecules, therefore, represents a challenge in facilitating enantiomer analysis. In this article, we describe how a combination of techniques ranging from organic synthesis to molecular biology yields antibodies of predetermined specificity and stereoselectivity that can be used as tailor-made chiral selectors for the chromatographic separation of enantiomers and their sensitive detection in immunosensors.

关键词：手性化合物手性选择剂对映体分离检测方法

Function and regulation of self-reactive marginal zone B cells in autoimmune arthritis

在线全文

学校读者我要写书评

暂无评论

Cellular & Molecular Immunology 2015年第4期12卷 493-503页

作者： Anna-Karin e Palm heike C Friedrich Anja Mezger Maya Salomonsson Linda K Myers Sandra Kleinau Department of Cell and Molecular Biology Uppsala University Uppsala Sweden Department of Pediatrics University of Tennessee Health ScienceCenter Memphis TN USA

Polyreactive innate-type B cells account for many B cells expressing self-reactivity in the periphery. Improper regulation of these B cells may be an important factor that underlies autoimmune disease. Here we have explored the influence of self-reactive innate B cells in the development of collagen-induced arthritis （CIA）, a mouse model of rheumatoid arthritis. We show that splenic marginal zone （MZ）, but not B- 1 B cells exhibit spontaneous IgM reactivity to autologous collagen II in na＇=＇ve mice. Upon immunization with heterologous collagen II in complete Freund＇s adjuvant the collagen-reactive MZ B cells expanded rapidly, while the B-1 B cells showed a modest anti-collagen response. The MZ B cells were easily activated by toll-like receptor （TLR） 4 and 9-1igands in vitro, inducing proliferation and cytokine secretion, implying that dual engagement of the B-cell receptor and TLRs may promote the immune response to self-antigen. Furthermore, collagen-primed MZ B cells showed significant antigen-presenting capacity as reflected by cognate T-cell proliferation in vitroand induction of IgG anti-collagen antibodies in vivo. MZ B cells that were deficient in complement receptors I and 2 demonstrated increased proliferation and cytokine production, while Fcy receptor lib deficiency of the cells lead to increased cytokine production and antigen presentation. In conclusion, our data highlight self-reactive MZ B cells as initiators of the autoimmune response in CIA, where complement and Fc receptors are relevant in controlling the self-reactivity in the cells.

关键词： arthritis complement receptors Fc receptors marginal zone B cells, mice

exotic tree seedlings are much more competitive than natives but show underyielding when growing together

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Plant ecology 2013年第4期6卷 305-315页

作者： heike Kawaletz Inga Mölder Stefan Zerbe Peter Annighöfer André Terwei Christian Ammer Department of Silviculture and Forest ecology of the Temperate Zones University of GöttingenBüsgenweg 137077 GöttingenGermany energieagentur Region Göttingen e.V. Berliner Str.237073 GöttingenGermany Faculty of Science and Technology Free University of Bozen-BolzanoPiazza Università539100 BolzanoItaly

Aims Invasive species continue to be a worldwide threat to ecosystems mainly as a cause for biodiversity *** ecosystems,for example,are subject to a change in species composition due to the invasion of exotic *** the attributes that cause the strong competitiveness of several exotic species may improve the ability to understand and effectively manage plant invasions in the *** this study the following hypotheses were tested:(1)biomass production of below-and aboveground plant compo-nents of the exotic tree species is higher than that of the natives,resulting in a higher competitiveness of the exotics;(2)the exclu-sion of root competition has a positive effect on the biomass pro-duction of the inferior native species;and(3)mixtures of native and exotic species yield a higher biomass production than the respective *** a pot experiment,containing about 2000 tree seedlings,was *** investigated the biomass productivity and growth reactions of two native(Quercus robur l.,Carpinus betulus l.)and two exotic tree species(Prunus serotina ehrh.,Robinia pseudoacacia l.)in different intra-and interspecific,competitive situations with and without the influence of root *** Findingsthe biomass production of both exotic species was significantly higher and led to a strong competitive advantage,resulting in a biomass decrease of the less competitive native *** high belowground biomass of both exotic species had a negative effect on the biomass *** competitive pressure of exotic tree seedlings on the native ones was largely driven by root ***,mixtures of native and exotic tree species had a higher productivity than their growth in monocultures would have *** was lower for exotic species in mixtures with the less productive native species compared to the competition in monocultures or in mixture with the other highly productive exotic ***,b

关键词： competition biomass production pot experiment invasive species Prunus serotina Robinia pseudoacacia

High stability supercontinuum generation in lead silicate SF57 photonic crystal fibers

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Physics B 2013年第1期22卷 303-306页

作者：朱星平李曙光杜颖韩颖张闻起阮银兰 heike ebendorff-Heidepriem Shahraam Afshar Tanya M. Monro Key Laboratory of Metastable Materials Science and Technology College of Science Yanshan University The Institute for Photonics & Advanced Sensing School of Chemistry & Physics The University of Adelaide Adelaide SA 5005 Australia

We report supercontinuum （SC） generation in a lead silicate SF57 photonic crystal fiber by using a 1550 nm pump source. The effective nonlinear coefficient of the SF57 fiber is simulated to be 111.5 W-1 .km- 1 at 1550 nm. The fiber also shows ultraflat dispersion from 1700 nm to 2100 nm. Our results reveal that with an increase of the average power of the incident pulse from 10 mW to 90 mW, the SC of the SF57 photonic crystal fiber is generated from 1300 nm to 1900 nm with high stability and without significant change in spectral broadening.

关键词： photonic crystal fiber nonlinear optics supercontinuum generation

Biased dispersal of Metrioptera bicolor, a wing dimorphic bush-cricket

在线全文

学校读者我要写书评

暂无评论

Insect Science 2018年第2期25卷 297-308页

作者： Ina Monika Margret Heidinge1,4, Silke Hein2, heike Feldhaar3 and Hans-Joachim Poethke1 Field Station Fabrikschleichach University of Würzburg Rauhenebrach Germany Bavarian State Institute for Viticulture and Horticulture Bee Research Center An der Steige 15 97206 Veitshöchheim Germany Wässeri 8 CH‐8816 Hirzel Switzerland Department of Animal ecology I University of Bayreuth Bayreuth Germany

In the highly fragmented landscape of central europe, dispersal is of particular importance as it determines the long-term survival of animal populations. Dispersal not only secures the recolonization of patches where populations went extinct, it may also rescue small populations and thus prevent local extinction events. As dispersal involves different individual fitness costs, the decision to disperse should not be random but context- dependent and often will be biased toward a certain group of individuals （e.g., sex- and wing morph-biased dispersal）. Although biased dispersal has far-reaching consequences for animal populations, immediate studies of sex- and wing morph-biased dispersal in orthopterans are very rare. Here, we used a combined approach of morphological and genetic analyses to investigate biased dispersal of Metrioptera bicolor, a wing dimorphic bush-cricket. Our results clearly show wing morph-biased dispersal for both sexes of M. bicolor. In addition, we found sex-biased dispersal for macropterous individuals, but not for micropters. Both, morphological and genetic data, favor macropterous males as dispersal unit of this bush-cricket species. To get an idea of the flight ability ofM. bicolor, we compared our morphological data with that of Locusta migratoria and Schistocerca gregaria, which are very good flyers. Based on our morphological data, we suggest a good flight ability for macropters of M. bicolor, although flying individuals of this species are seldom observed.

关键词： aspect ratio dispersal traits flight muscle ratio Metrioptera bicolor sex-biased dispersal wing load wing morph-biased dispersal

The unique ORF8 protein from SARS-CoV-2 binds to human dendritic cells and induces a hyper-inflammatory cytokine storm

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Molecular Cell Biology 2023年第10期15卷 39-53页

作者： Matthias Hamdorf Thomas Imhof Ben Bailey-elkin Janina Betz Sebastian JTheobald Alexander Simonis Veronica Di Cristanziano Lutz Gieselmann Felix Dewald Clara Lehmann Max Augustin Florian Klein Miguel AAlejandre Alcazar Robert Rongisch Mario Fabri Jan Rybniker heike Goebel Jörg Stetefeld Bent Brachvogel Claus Cursiefen Manuel Koch Felix Bock Cornea Lab experimental Ophthalmology Department of OphthalmologyFaculty of Medicine and University Hospital Cologne50937 CologneGermany Terasaki Institute for Biomedical Innovation Los AngelesCA 90024USA Center for Biochemistry Faculty of Medicine and University Hospital Cologne50931 CologneGermany Institute for experimental Dentistry and Oral Musculoskeletal Biology Faculty of Medicine and University Hospital Cologne50931 CologneGermany Department of Microbiology University of ManitobaWinnipeg MB R3B 2E9 ManitobaCanada Department I of Internal Medicine Division of Infectious DiseasesFaculty of Medicine and University Hospital Cologne50937 CologneGermany Center for Molecular Medicine Cologne University of Cologne50931 CologneGermany Institute of Virology Faculty of Medicine and University Hospital Cologne50935 CologneGermany German Center for Infection Research(DZIF) Partner Site Bonn-Cologne50931 CologneGermany Department of Children and Adolescent Medicine Faculty of Medicine and University Hospital Cologne50931 CologneGermany Cologne excellence Cluster Stress Responses in Aging-associated Diseases 50931 CologneGermany Institute for Lung Health(ILH) Universities of Gießen and Marburg Lung CentreMember of the German Center for Lung Research35392 GießenGermany Dermatology Faculty of Medicine and University Hospital Cologne50937 CologneGermany Institute of Pathology Faculty of Medicine and University Hospital Cologne50937 CologneGermany Department of Pediatrics and Adolescent Medicine Experimental NeonatologyFaculty of Medicine and University Hospital Cologne50931 CologneGermany

The novel coronavirus pandemic,first reported in December 2019,was caused by the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).SARS-CoV-2 infection leads to a strong immune response and activation of antigen-presenting cells,which can elicit acute respiratory distress syndrome(ARDS)characterized by the rapid onset of widespread inflammation,the so-called cytokine *** response to viral infections,monocytes are recruited into the lung and subsequently differentiate into dendritic cells(DCs).DCs are critical players in the development of acute lung inflammation that causes ***,we focus on the interaction of a specific SARS-CoV-2 open reading frame protein,ORF8,with *** show that ORF8 binds to DCs,causes pre-maturation of differentiating DCs,and induces the secretion of multiple proinflammatory cytokines by these *** addition,we identified DC-SIGN as a possible interaction partner of ORF8 on *** of ORF8 leads to reduced production of IL-1β,IL-6,IL-12p70,TNF-α,MCP-1(also named CCL2),and IL-10 by ***,a neutralizing antibody blocking the ORF8-mediated cytokine and chemokine response could be an improved therapeutic strategy against SARS-CoV-2.

关键词： COVID-19 SARS-CoV-2 ORF8 cytokine storm dendritic cells

Changes in Regional Potential Vegetation in Response to an Ambitious Mitigation Scenario

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of environmental Protection 2013年第8期4卷 16-26页

作者： heike Huebener Janina Korper Hessian Agency for environment and Geology WiesbadenGermany Freie Universitat Berlin Institut fur MeteorolgieBerlinGermany

Climate change impacts on the potential vegetation (biomes) are compared for an ambitious emissions-reduction scenario (e1) and a medium-high emissions scenario with no mitigation policy (A1B). The e1 scenario aims at limiting global mean warming to 2°C or less above pre-industrial temperatures and is closely related to the RCP2.6 sued in the CMIP5. A multi-model ensemble of ten state-of-the-art coupled atmosphere-ocean general circulation models (GCMs) is analyzed. A simple biome model is used to assess the response of potential vegetation to the different forcing in the two scenarios. Changes in biomes in response to the simulated climate change are less pronounced in e1 than in the A1B scenario. Most biomes shift polewards, with biomes adapted to colder climates being replaced by biomes adapted to warmer climates. In some regions cold biomes (e.g. Tundra, Taiga) nearly disappear in the A1B scenario but are also significantly reduced under the e1 scenario.

关键词： Climate Change Mitigation Scenario Potential Vegetation

Spatio-temporal changes of exposure and vulnerability to floods in China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Advances in Climate Change Research 2014年第4期5卷 197-205页

作者： WANG Yan-Jun GAO Chao ZHAI Jian-Qing LI Xiu-Cang SU Bu-da HARTMANN heike School of Geography and Remote Sensing Collaborative Innovation Center on Forecast and evaluation of Meteorological Disasters Nanjing University ofInformation Science & Technology College of Territorial Resources and Tourism Anhui Normal University National Climate Center China Meteorological Administration Department of Geography Geologyand the EnvironmentSlippery Rock University of Pennsylvania

A socio-economic data set on China's historical flood losses for the period 1984e2012 was compiled to analyze the exposed population,economy,and crop area as well as the vulnerabilities of the population and economy to *** results revealed that the exposed population was approximately 126 persons km 2per year when taking China as a whole;in terms of the economy,potential losses due to floods were estimated to be approximately 1.49 million CN$km 2and the crop area exposed to floods covered 153 million hm2per ***'s total exposure to floods significantly increased over the analysis *** areas that showed the higher exposure were mainly located along the east *** population's vulnerability to floods showed a significantly increasing trend,however,the economic vulnerability showed a decreasing *** populations and economies that were most vulnerable to floods were in Hunan,Anhui,Chongqing,Jiangxi,and Hubei *** municipalities of Shanghai,Beijing,and Tianjin showed the lowest vulnerabilities to floods.

关键词：洪水损失弱性中国时空变化经济数据曝光洪涝灾害损失估计

The Isogene 1-Deoxy-D-Xylulose 5-Phosphate Synthase 2 Controls Isoprenoid Profiles, Precursor Pathway Allocation, and Density of Tomato Trichomes

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2010年第5期3卷 904-916页

作者： heike Paetzold Stefan Garms Stefan Bartram Jenny Wieczorek eva-Maria Uros-Gracia Manuel Rodriguez-Concepcion Wilhelm Boland Dieter Strack Bettina Hause Michael H. Walter Leibniz Institut for Pflanzenbiochemie Abteilung Sekundarstoffwechsel Weinberg 3 D-06120 Halle (Saale) Germany Max-Planck-Institut fur Chemische Okologie Abteilung Bioorganische Chemie Hans-Knoll-Straβe 8 D-07745 Jena Germany Center for Research in Agricultural Genomics (CRAG) CSlC-IRTA-UAB Jordi Girona 18 08034 Barcelona Spain

Plant isoprenoids are formed from precursors synthesized by the mevalonate （MVA） pathway in the cytosol or by the methyl-D-erythritol 4-phosphate （MeP） pathway in plastids. Although some exchange of precursors occurs, cytosolic sesquiterpenes are assumed to derive mainly from MVA, while plastidial monoterpenes are produced preferentially from MeP precursors. Additional complexity arises in the first step of the MeP pathway, which is typically catalyzed by two divergent 1-deoxy-D-xylulose 5-phosphate synthase isoforms （DXS1, DXS2）. In tomato （Solanum lycopersicum）, the SIDXS1 gene is ubiquitously expressed with highest levels during fruit ripening, whereas SIDXS2 transcripts are abundant in only few tissues, including young leaves, petals, and isolated trichomes. Specific down-regulation of SIDXS2 expression was performed by RNA interference in transgenic plants to investigate feedback mechanisms. SIDXS2 down-regulation led to a decrease in the monoterpene β-phellandrene and an increase in two sesquiterpenes in trichomes. Moreover, incorporation of MVA-derived precursors into residual monoterpenes and into sesquiterpenes was elevated as determined by comparison of ^13C to ^12C natural isotope ratios. A compensatory up-regulation of SIDXS1 was not observed. Down-regulated lines also exhibited increased trichome density and showed less damage by leaf-feeding Spodoptera littoralis caterpillars. The results reveal novel, non-redundant roles of DXS2 in modulating isoprenoid metabolism and a pronounced plasticity in isoprenoid precursor allocation.

关键词： Isoprenoid biosynthesis methyI-D-erythritol 4-phosphate (MeP) pathway 1-deoxy-D-xylulose 5-phosphate synthase 2 (DXS2) RNA interference (RNAi) trichomes cross-talk feedback regulation GC-C-IRMS.