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A High-throughput Genomic Tool： Diversity Array Technology Complementary for Rice Genotyping

Journal of Integrative Plant Biology 2006年第9期48卷 1069-1076页

作者： Yong Xie Kenneth McNally Cheng-Yun Li hei leung You-Yong Zhu National Center for Agro-biodiversity Yunnan Agricultural University Kunming 650201 China Genetic Resources Center International Rice Research Institute DAPO P.O. Box 7777 Metro Manila Philippines Entomology and Plant Pathology Division International Rice Research Institute DAPO P.O. Box 7777 Metro Manila Philippines

Diversity array technology （DART^TM） was a genotyping tool characterized gel-independent and high throughput. The main purpose of present study is to validate DArT for rice （Oryza sativa L.）genotyping in a high throughput manner. Technically, the main objective was to generate a rice general purpose gene pool, and optimize this genomic tool in order to evaluate rice germplasm genetic diversity. To achieve this, firstly, a generalpurpose DArT array was developed. Ten representatives from 24 varieties were hybridized with the general-purpose array to determine the informativeness of the clones printed on the array. The informative 1 152 clones were re-arrayed on a slide and used to fingerprint 17 of 24 germplasms. Hybridizing targets prepared from the germplasm to be assayed to the DNA array gave DNA fingerprints of germplasms. Raw data were normalized and transformed into binary data, which were then analyzed by using NTSYSpc （Numerical taxonomy system for cluster and ordination analysis, v. 2.02j） software package. The graphically displayed dendrogram derived from the array experimental data was matched with simple sequence repeats genotyping outline and varieties＇ pedigree deviation of the different varieties. Considering DArT is a sequence-independent genotyping approach, it will be applied in studies of the genetic diversity and the gene mapping of diverse of organisms, especially for those crops with less-developed molecular markers.

关键词： amplified fragment length polymorphisms diversity array technology genome complexity reduction microarray rice genotyping

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Dual ultra-wideband(UWB)radar-based sleep posture recognition system:Towards ubiquitous sleep monitoring

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Engineered Regeneration 2023年第1期4卷 36-43页

作者： Derek Ka-hei Lai Li-Wen Zha Tommy Yau-Nam leung Andy Yiu-Chau Tam Bryan Pak-hei So Hyo-Jung Lim Daphne Sze Ki Cheung Duo Wai-Chi Wong James Chung-Wai Cheung Department of Biomedical Engineering Faculty of EngineeringThe Hong Kong Polytechnic UniversityHong Kong 999077China Department of Bioengineering Imperial CollegeLondon SW72AZUnited Kingdom Research Institute of Smart Ageing The Hong Kong Polytechnic UniversityHong KongChina School of Nursing The Hong Kong Polytechnic UniversityHong KongChina

Sleep posture monitoring is an essential assessment for obstructive sleep apnea(OSA)*** objective of this study is to develop a machine learning-based sleep posture recognition system using a dual ultra-wideband radar *** collected radiofrequency data from two radars positioned over and at the side of the bed for 16 patients performing four sleep postures(supine,left and right lateral,and prone).We proposed and evaluated deep learning approaches that streamlined feature extraction and classification,and the traditional machine learning approaches that involved different combinations of feature extractors and *** results showed that the dual radar system performed better than either single *** statistical features with random forest classifier yielded the best accuracy(0.887),which could be further improved via an ablation study(0.938).Deep learning approach using transformer yielded accuracy of 0.713.

关键词： Obstructive sleep apnea Deep learning Sleep monitoring Feature extraction Ablation study

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稻瘟病菌体细胞色素的变异性及其突变体

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中国水稻科学 1992年第2期6卷 83-88页

作者：沈瑛朱培良袁筱萍 hei leung 中国水稻研究所杭州310006 国际水稻研究所

利用紫外光诱变,从来自菲律宾田间的一个具有广谱毒性的流行菌株PO6—6中获得一些浅黄色素突变株,并检测了它侵染水稻的频率;同时对利用浅黄色的表现型来研究体细胞变异的可能性进行了探讨。在所测试的13个浅黄色素突变株中,有6个能使... 详细信息

利用紫外光诱变,从来自菲律宾田间的一个具有广谱毒性的流行菌株PO6—6中获得一些浅黄色素突变株,并检测了它侵染水稻的频率;同时对利用浅黄色的表现型来研究体细胞变异的可能性进行了探讨。在所测试的13个浅黄色素突变株中,有6个能使水稻产生病斑。在品种CO39、IR50、IR36上能重新分离到浅黄色素突变株。由此表明,某些浅黄色的突变株具有侵染水稻的能力,它们没有通常的黑色素合成。从一些浅黄色突变株引起的病斑上获得的单孢培养菌中,野生菌株(Wild type)色素的频率范围为10％～100％。由浅黄色突变株引起的病斑中也可重新获得白色的变异物。在室内培养试验中,出现色素变异的频率未超出10^(-3)～10^(-4)的范围。用一个浅黄色突变株和野生类型的分离菌株进行的相互接种中,从品种IR50的病斑上分离的单孢中,浅黄色菌株占绝对优势。在一次实验中,由浅黄色素培养菌所引起的一个病斑中,同时获得了浅黄色和灰黑色的培养菌。这些结果表明,体细胞色素的变异也许表现在与寄主植物之间的互作。

关键词：体细胞色素变异性

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The Genomic Variation of 3,000 diverse Rice (Oryza sativa L.)Accessions:Discoveries And Applications In Rice Improvement

The Genomic Variation of 3,000 diverse Rice (Oryza sativa L....

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2018中国作物学会学术年会

作者：黎志康 Fan Zhang Whensheng Wang Ramil Mauleon Zhiqiang Hu Dmytro Chebotarov Shuaishuai Tai Zhichao Min Li Tianqing Zheng Roven Rommel Fuentes Locedie Mansueto Dario Copetti Millicent Sanciangco Kevin Christian Palis Jianlong Xu Chen Sun Hongliang Zhang Binying Fu Yongming Gao Xiuqin Zhao Fei Shen Xiao Cui Hong Yu Zichao Li Miaolin Chen Jeffery Detras Yongli Zhou Xinyuan Zhang Yue Zhao Dave Kudrna Chunchao Wang Rui Li Ben Jia Jinyuan Lu Xianchang He Zhaotong Dong Jiabao Xu Yanhong Li Miao Wang Jianxin Shi Jing Li Dabing Zhang Seunhee Lee Wushu Hu Alexander Poliakov Inna Dubchak Victor Jun Ulat Frances Nikki Borja John Robert Mendoza Jauhar Ali Ming Yang Yongchao Niu Zhen Yue Ma.Elizabeth B.Naredo Jayson Talag Xueqiang Wang Jinjie Li Xiaodong Fang Ye Yin Jean-Christophe Glaszmann Jianwei Zhang Jiayang Li Ruaraidh Sackville Hamilton Rod A.Wing Chaochun Wei Jue Ruan Gengyun Zhang Kenneth L.Mcnally Nickolai Alexandrov hei leung hei Institute of Crop Sciences Chinese Academy of Agricultural Sciences Shenzhen Institute for Innovative Breeding Chinese Academy of Agricultural Sciences International Rice Research Institute School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai Center for Bioinformation Technology BGI Genomics BGI-Shenzhen Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Anhui Agricultural University Arizona Genomics Institute School of Plant SciencesUniversity of Arizona China Agricultural University Institute of Genetics and Developmental Biology Chinese Academy of Sciences Lawrence Berkeley National Laboratory

Asian cultivated rice(Oryza sativa L.) is the staple food for the half world population with rich within species *** analyses of the genome resequencing data of a core collection of 3,010 rice accessions revealed four important aspects of the genomic diversity within ***,over 42 million SNPs and >3 million small InDels were discovered against five reference ***,we discovered more than 90,000 structural(>100 bps) variations(SVs) when compared with the Geng reference genome,consisting primarily of small-sized deletions and translocations,plus many duplications and *** SVs accounted for significant portion of the rice genome and involved most(～80%) rice ***,we discovered >12,000 full-length novel protein-coding genes plus 9000+ novel genes of partial sequences that are absent in the Nipponbare reference *** rice pan genome consists of ～62%core genes/gene families that are present in all rice lines,and ～38%distributed genes that are present only some of rice ***,using large-effect SNPs and InDels of all loci in the PG,a rice functional haplotype database was *** SNP,PG and SV variation clearly resolve the 3,010 rice accessions into two major subspecies,Xian(indica) and Geng(japonica),plus Aus and Aro groups and multiple subpopulations within Xian and *** reduced diversity was found in genomic regions where over 1,000 agronomically important and domestication-related genes *** efforts have been taken to identify and mine genes/QTLs/alleles associated with a wide range of important traits in rice using the 3 k materials and SNP *** demonstrate how the results of the 3 k RGP could be used in rice improvement,genomic constitutions of 476 BC F introgression lines(ILs) from our breeding program were constructed using high-quality reference genomes of their 9 parents and the 3 k RGP *** compared with their recurrent parent,Huang-hua-

关键词： Rice genome diversity population structure pan genomes genomics-based breeding by design

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Genetic Analysis and Gene Mapping of Light Brown Spotted Leaf Mutant in Rice

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Rice science 2013年第1期20卷 13-18页

作者： FENG Bao-hua YANG Yang SHI Yong-feng LIN Lu CHEN Jie WEI Yan-lin hei leung WU Jian-li State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement China National Rice Research Institute College of Life and Environmental Sciences Hangzhou Normal University College of Life Sciences Anhui Normal University International Rice Research Institute DAPO Box 7777 Metro ManilaPhilippines

A light brown spotted-leaf mutant of rice was isolated from an ethane methyl sulfonate （EMS）- induced IR64 mutant bank. The mutant, designated as Ibsll （light brown spotted-leaf 1）, displayed light brown spot in the whole growth period from the first leaf to the flag leaf under natural summer field conditions. Agronomic traits including plant height, growth duration, number of filled grains per panicle, seed-setting rate and 1000-grain weight of the mutant were significantly affected. Genetic analysis showed that the mutation was controlled by a single recessive gene, tentatively named Ibsll（t）, which was mapped to the short arm of chromosome 6. By developing simple sequence repeat （SSR） markers, the gene was finally delimited to an interval of 130 kb between markers RM586 and RM588. The Ibsll（t） gene is likely a novel rice spotted-leaf gene since no other similar genes have been identified near the chromosomal region. The genetic data and recombination populations provided will facilitate further fine-mapping and cloning of the gene.

关键词： Oryza sativa leaf color spotted-leaf molecular marker gene mapping

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Predicting eczema severity beyond childhood

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World Journal of Pediatrics 2016年第1期12卷 44-48页

作者： Kam Lun Hon Yin-Ching K.Tsang Terence Chuen W.Poon Nga Hin Pong Matthew Kwan Shirley Lau Yuen-Chun Chiu Hin-hei Wong Ting-Fan leung Department of Pediatrics The Chinese University of Hong KongPrince of Wales HospitalHong KongChina Faculty of Medicine The Chinese University of Hong KongChina

Background:We evaluated factors associated with eczema severity in ***:Nottingham Eczema Severity Score(NESS),family and personal history of atopy,skin prick test for common food and aeroallergens,highest serum IgE level and eosinophil count were *** with paired NESSs(childhood-NESS is NESS performed at10 years)were further ***:Adolescence-NESS(n=383 patients)was associated with eczema onset in infancy,dust mite and food allergen sensitization,dietary avoidance,use of wet wrap,traditional Chinese medicine,immunomodulant(azathioprine or cyclosporine),high IgE level,eosinophil count,but not with family/personal history of ***-two patients had both childhood-NESS and adolescence-NESS(mean follow-up of 6.8 years)showing that adolescence-NESS was associated with childhood-NESS severity grades(P=0.034).Of these patients,48%remained in the same severity grades,whereas 39%improved,and 13%deteriorated from childhood to ***:It is not possible to assure parents that their child can outgrow *** eczema prognosis research,long-term follow-up is warranted.

关键词： atopic dermatitis atopy eczema Nottingham Eczema Severity Score prognosis

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CO39近等基因系抗稻瘟病性分析

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作物学报 2001年第6期27卷 838-841页

作者：何月秋唐文华 hei leung RobertS.Zeigler 云南农业大学云南省植物病理重点实验室云南昆明650201 中国农业大学植物病理系北京100094 国际水稻研究所昆虫植病系

利用 14个稻瘟病菌菌株在温室分别接种和在自然病圃两个年度对 CO39近等基因系进行鉴定。结果表明 ,这套近等单基因系和近等多基因系具很好的鉴别能力。抗病基因 Pi- 3提供抗病性的时间较长 ,病斑数较少。

关键词：近等基因系稻瘟病抗病性 CO39 水稻遗传性

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Pot 2在稻瘟病菌群体结构分析中的应用

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华中农业大学学报 2000年第4期19卷 317-321页

作者：何月秋唐文华2 hei leung Robert S.Zeigler 国际水稻研究所昆虫植病系中国农业大学植物病理系北京100094

利用稻瘟病菌的一段倒位重复序列Pot2设计的一对引物直接对稻瘟病菌的DNA进行PCR扩增。根据带型相似性大于 80 %为度可将国际水稻所一个稻瘟病圃上的 31 0个菌株划分 6个宗亲群 (lineage)。这些依分子标记划分的宗亲群与品种的遗传背景... 详细信息

利用稻瘟病菌的一段倒位重复序列Pot2设计的一对引物直接对稻瘟病菌的DNA进行PCR扩增。根据带型相似性大于 80 %为度可将国际水稻所一个稻瘟病圃上的 31 0个菌株划分 6个宗亲群 (lineage)。这些依分子标记划分的宗亲群与品种的遗传背景有密切关系 ,而且各宗亲群在水稻不同生育期有不同的波动方式。

关键词： Pot 2 稻瘟病菌群体结构

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三黄占2号稻瘟病抗性与稻米直链淀粉含量的关系研究

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作物学报 2006年第2期32卷 159-163页

作者：张少红刘斌朱小源杨健源伍尚忠 hei leung 广东省农业科学院水稻研究所广东广州510640 广东省农业科学院植物保护研究所广东广州510640 菲律宾国际水稻研究所

以高抗稻瘟病、直链淀粉含量(AC)较高的三黄占2号和高感稻瘟病、AC较低的丽江新团黑谷衍生的重组自交系群体为研究材料,从性状的相关性和控制两性状的基因在染色体上的位置关系剖析稻瘟病抗性和稻米AC的内在关系。结果表明,两性状间没... 详细信息

以高抗稻瘟病、直链淀粉含量(AC)较高的三黄占2号和高感稻瘟病、AC较低的丽江新团黑谷衍生的重组自交系群体为研究材料,从性状的相关性和控制两性状的基因在染色体上的位置关系剖析稻瘟病抗性和稻米AC的内在关系。结果表明,两性状间没有显著的相关性。3个与AC相关的QTL分别被定位在第5、6和7染色体上,其加性效应均来自丽江新团黑谷,起降低AC的作用。比较这些QTL与先前对同一群体鉴定的稻瘟病抗性基因(主效基因和QTL)在染色体上的位置,表明控制这两性状的基因没有紧密连锁关系,亦没有显著的基因间互作。据此认为,通过亲本的合理选择和分子标记辅助选择,可以把三黄占2号稻瘟病持久抗性与理想AC整合到同一品系中,育成优质、抗病的优良品种。

关键词：稻瘟病抗性直链淀粉含量基因定位相关性水稻

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一种用于水稻点突变检测的PCR引物设计方法

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中国水稻科学 2007年第2期21卷 215-219页

作者：邱福林邵国军 hei leung 吴建利程式华沈阳农业大学辽宁沈阳110161 辽宁省农业科学院稻作研究所辽宁沈阳110101 国际水稻研究所昆虫和植物病理系中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室浙江杭州310006

介绍了一种用于水稻点突变检测的PCR引物设计方法。利用该方法根据12个候选基因序列设计了12对引物,其中10对引物可以正常工作,能从基因组中扩增出特异性条带,合格率为83%,并且成功地在突变体中检测到点突变。这一方法不仅可以用于水稻... 详细信息

介绍了一种用于水稻点突变检测的PCR引物设计方法。利用该方法根据12个候选基因序列设计了12对引物,其中10对引物可以正常工作,能从基因组中扩增出特异性条带,合格率为83%,并且成功地在突变体中检测到点突变。这一方法不仅可以用于水稻,也可以用于其他动植物。同时,对引物设计过程中常见问题进行了讨论并给出了解决办法。

关键词：水稻定向诱导基因组局部突变点突变检测引物设计聚合酶链式反应

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