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The transcription factor ZEB1 promotes chemoresistance in prostate cancer cell lines

Asian Journal of Andrology 2019年第5期21卷 460-467页

作者： Octavio Orellana-Serradell Daniela Herrera Enrique A Castellon hector r contreras Department of Basic and Clinical Oncology Faculty of MedicineUniversity of ChileIndependenciaSantiago 8380453Chile

One of the factors promoting tumoral progress is the abnormal activation of the epithelial-mesenchymal transition(EMT)program which has been associated with chemoresistance in tumoral *** transcription factor zinc finger E-box-binding homeobox 1(ZEB1),a key EMT activator,has recently been related to docetaxel resistance,the main chemotherapeutic used in advanced prostate cancer *** mechanisms involved in this protective effect are still *** a previous work,we demonstrated that ZEB1 expression induced an EMT-like phenotype in prostate cancer cell *** this work,we used prostate cancer cell lines 22rvl and DU145 to study the effect of ZEB1 modulation on docetaxel resistance and its possible *** results showed that ZEB1 overexpression conferred to 22rvl cell resistance to docetaxel while its silencing made DU145 cells more sensitive to *** of resistance markers showed no presenee of ATP-binding cassette subfamily B member 1(MDr1)and no changes in breast cancer resistance protein(BCrP)or ATP-binding cassette subfamily C member 10(MrP7).However,a correlation between ZEB1,multidrug resistance-associated protein 1(MrP1),and ATP-binding cassette subfamily C member 4(MrP4)expression was ***4 inhibition,using MK571,resensitized cells with ZEB1 overexpression to docetaxel *** addition,modulation of ZEB1 and subsequent change in MrP4 expression correlated with a lower apoptotic response to docetaxel,characterized by lower B-cell lymphoma 2(Bcl2),high BCL2-associated X protein(Bax),and high active caspase 3 *** response to docetaxel in our model seems to be mediated mainly by activation of the apoptotic death *** results showed that modulation of MrP4 could be a mediator of ZEBl-related resistance to docetaxel in prostate cancer,making it a possible marker for chemotherapy response in patients who do not express MDr1.

关键词： chemoresistance prostate cancer ZEB1

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The transcription factor ZEB1 promotes an aggressive phenotype in prostate cancer cell lines

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Asian Journal of Andrology 2018年第3期20卷 294-299页

作者： Octavio Orellana-Serradell Daniela Herrera Enrique A Castellon hector r contreras Department of Basic and Clinical Oncology Faculty of Medicine University of Chile Independencia Santiago 8380453 Chile.

It has been reported that one of the factors that promotes tumoral progression is the abnormal activation of the epithelial-mesenchymal transition program. This process is associated with tumoral cells acquiring invasive and malignant properties and has the transcription factor zinc finger E-box-binding homeobox 1 （ZEB1） as one of its main activators. However, the role of ZEB1 in promoting malignancy in prostate cancer （PC, a） is still unclear. Here, we report that ZEB1 expression correlates with Gleason score in PCa samples and that expression of ZEB1 regulates epithelial-mesenchymal transition and malignant characteristics in PCa cell lines. The results showed that ZEB1 expression is higher in samples of higher malignancy and that overexpression of ZEB1 was able to induce epithelial-mesenchymal transition by upregulating the mesenchymal marker Vimentin and downregulating the epithelial marker E-Cadherin. On the contrary, ZEB 1 silencing repressed Vimentin expression and upregulated E-Cadherin. ZEB1 expression conferred enhanced motility and invasiveness and a higher colony formation capacity to 22rvl cells whereas DU145 cells with ZEB1 silencing showed a decrease in those same properties. The results showed that ZEB1 could be a key promoter of tumoral progression toward advanced stages of PC, a.

关键词： epithelial-mesenchymal transition prostate cancer transcriptional repression ZEB1

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Secreted protein acidic and rich in cysteine(SPArC)induces epithelial-mesenchymal transition,enhancing migration and invasion,and is associated with high Gleason score in prostate cancer

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Asian Journal of Andrology 2019年第6期21卷 557-564页

作者： Fernanda Lopez-Moncada Maria Jose Torres Enrique A Castellon hector r contreras Department of Basic and Clinic Oncology Faculty of Medic泊eUniversity of ChileSantiago 8389100Chile

Secreted protein acidic and rich in cysteine(SPArC)is a matricellular protein highly expressed in bone tissue that acts as achemoattractant factor promoting the arrival of prostate cancer(PCa)cells to the bone ***,the contribution of SPArCduring the early stages of tumor progression remains *** this study,we show that SPArC is highly expressed in PCa tissueswith a higher Gleason *** stable knockdown and overexpression of SPArC in PC3 and LNCaP cells,respectively,here wedem on strate that en doge nous SPArC induces the epithelial-mesenchymal tran sition(EMT),decreasing E-cadheri n and cytokeratin18 and increasing N-cadheri n and vime ***,SPArC in duces the expression of EMT regulatory tran scription factors Snailfamily transcriptional repressor 1(Snail),Snail family transcriptional repressor 2(Slug),and zinc finger E-box binding homeobox 1(Zeb1).In addition,SPArC knockdown in PC3 cells decreases migration and invasion in vitro,without modifying cell *** results indicate that SPArC might facilitate tumor progression by modifying the cellular phenotype in cancer cells.

关键词： epithelial-mesenchymal transition invasion migration prostate cancer secreted protein acidic and rich in cysteine(SPArC)

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syndecan-1和-2表达形式的变化可以预测前列腺癌生化复发

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Asian Journal of Andrology 2011年第3期13卷 476-480,514页

作者： rodrigo Ledezma Federico Cifuentes Ivan Gallegos Juan Fulla Enrique Ossandon Enrique A Castellon hector r contreras Laboratory of Molecular and Cellular Andrology Physiology and Biophysics Program. Institute of Biomedical Sciences Faculty of Medicine University of Chile Santiago 70005 Chile pathology Service Clinic Hospital University of Chile Santiago 70005 Chile Urology Service Clinic Hospital University of Chile Santiago 70005 Chile

The clinical features of prostate cancer do not provide an accurate determination of patients undergoing biochemical relapse and are therefore not suitable as indicators of prognosis for recurrence. New molecular markers are needed for proper pre-treatment risk stratification of patients. Our aim was to assess the value of altered expression of syndecan-1 and -2 as a marker for predicting biochemical relapse in patients with clinically localized prostate cancer treated by radical prostatectomy. The expression of syndecan-1 and -2 was examined by immunohistochemical staining in a series of 60 paraffin-embedded tissue samples from patients with localized prostate cancer. Ten specimens from patients with benign prostatic hyperplasia were used as non-malignant controls. Semiquantitative analysis was performed to evaluate the staining patterns. To investigate the prognostic value, Kaplan-Meier survival curves were performed and compared by a log-rank test. In benign samples, syndecan-1 was expressed in basal and secretory epithelial cells with basolateral membrane Iocalisation, whereas syndecan-2 was expressed preferentially in basal cells. In prostate cancer samples, the expression patterns of both syndecans shifted to granular-cytoplasmic Iocalisation. Survival analysis showed a significant difference （P〈0.05） between normal and altered expression of syndecan-1 and -2 in free prostate-specific antigen recurrence survival curves. These data suggest that the expression of syndecan-1 and -2 can be used as a prognostic marker for patients with clinically localized orostate cancer, improving the larostate-specific antigen recurrence risk stratification.

关键词： biochemical recurrence prostate cancer syndecans

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