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Allograft rejection-related gene expression in the endothelial cells of renal transplantation recipients after cytomegalovirus infection

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第11期10卷 820-828页

作者： Yang li Hang YAN Wu-jun XUE Pu-xun TIAN Xiao-ming DING Xiao-ming PAN Xin-shun FENG Xiao-hui TIAN he-li xiang Jun HOU Department of Renal Transplantation Center of Nephropathy the First Affiliated Hospital School of Medicine Xi 'an Jiaotong University Xi 'an 710061 China The two authors contributed equally to this work

Objective： To explore the effects of cytomegalovirus （CMV） infection on rejection-related gene expression in the endothelial cells of renal transplantation recipients. Methods： Endothelial cells （ECs） were cultured and stimulated by a variety of factors： A, normal control group; B, inactivated human cytomegalovirus （HCMV） infection group; C, HCMV infection group; D, HCMV supematant infection group; and E, ganciclovir HCMV group. Expression of intercellular adhesion molecule-1 （ICAM-1） and major histocompability complex （MHC） class I and class II antigens was detected by flow cytometry （FCM） and immuno- histochemistry. Results： We found characteristic CMV-infected ECs in this study. There were no significant differences among groups A, B and D （P〉0.05）. Although the expression levels of ICAM-1 were not significantly different between groups C and E （P〉0.05）, the ICAM-1 expression in these two groups was significantly higher than that in group A （P〈0.05）. ICAM-1 expression was detected in groups C and E, while there was no expression in groups A, B and D. Furthermore, there was no significant difference of ICAM-1 mRNA expression between groups C and E （P〉0.05）. Human leucocyte antigen （HLA）-ABC expression was detected in all the groups, while HLA-DR expression was only detected in groups C and E. There were no significant dif- ferences of HLA-ABC and HLA-DR expression among groups A, B and D （P〉0.05）. However, the HLA-ABC and HLA-DR expression levels in groups C and D were higher than those of the remaining groups previously reported （P〈0.05）. Meanwhile, the HLA-ABC and HLA-DR expression levels in group E were lower than those of group C （P〈0.05）. Conclusion： CMV could up-regulate the expression levels of ICAM-1 and MHC antigens, which was closely related to allograft rejection.

关键词： Renal transplantation Cytomegalovirus (CMV) Rejection Intercellular adhesion molecule-1 (ICAM-1) Major histocompability complex (MHC)

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The clinical value of enzyme-multiplied immunoassay technique monitoring the plasma concentrations of cyclosporine A after renal transplantation

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Journal of Pharmaceutical Analysis 2011年第2期1卷 139-142页

作者： Xiao-Hui Luo wu-Jun Xue Pu-Xun Tian Xiao-Ming Ding Hang Yan he-li xiang Yang li Department of Renal Transplant Center of Nephropathy the First Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710061 China.

The feasibility and the clinical value of the enzyme-multiplied immunoassay technique （EMIT） monitoring of blood concentrations of cyclosporine A （CsA） in patients treated with CsA were investigated after kidney transplantation. The validation method was performed to the EMIT determination of CsA blood concentration, the CsA whole blood trough concentrations （Co） of patients in different time periods after renal transplantation were monitored, and combined with the clinical complications, the statistical results were analyzed and compared. EMIT was precise, accurate and stable, also with a high quality control. The mean postoperative blood concentration of CsA was as follows：〈1 month, （281.4± 57.9）ng/mL; 2 - 3 months, （264.5 ± 41.2） ng/mL; 4 - 5 months, （236.4 ± 38.9） ng/mL; 6 - 12 months, （206.5± 32.6）ng/mL; 〉12 months, （185.6± 28.1）ng/mL. The toxic reaction rate of CsA blood concentration within the recommended therapeutic concentration was 14.1%, significantly lower than that of the none-recommended dose group （37.2%）（P〈0.05）; the transplantation rejection rate was 4.4%, significantly lower than that of the none- recommended dose group （22.5%）（P〈0.05）. Using EMIT to monitor the blood concentration of CsA as the routine laboratory method is feasible, and is able to reduce the CsA toxicity and rejection significantly, leading to achieving the desired therapeutic effect.

关键词： enzyme-multiplied immunoassay technique renal transplantation cyclosporin A blood concentration monitoring

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无限域波动问题的局部非协调网格法

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振动工程学报 2006年第1期19卷 124-127页

作者：贺向丽李同春河海大学水利水电工程学院江苏南京210098

基于远置人工边界的原理采用了局部非协调网格法,即以协调点集的位移、速度、加速度变量作为基本变量,非协调点集的变量作为从变量,通过线性插值建立从变量与基本变量之间的线性关系,从而导出以基本变量作为未知量的总体线性方程组。并... 详细信息

基于远置人工边界的原理采用了局部非协调网格法,即以协调点集的位移、速度、加速度变量作为基本变量,非协调点集的变量作为从变量,通过线性插值建立从变量与基本变量之间的线性关系,从而导出以基本变量作为未知量的总体线性方程组。并通过算例验证了本文方法的精度。本文方法不仅大大减少了计算量,提高了求解效率,而且避开了大角度入射和人工边界稳定性的问题,还可以用于其他人工边界的检验工作。

关键词：无限域波动远置人工边界局部非协调网格插值

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Chemoradiotherapy followed by surgery could improve the efficacy of treatments in patients with resectable esophageal carcinoma

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Chinese Medical Journal 2013年第16期126卷 3138-3145页

作者： WANG Feng WANG Ya-mei he Wei li xiang-ke PENG Fang-hui YANG Xiao-li FAN Qing-xia Department of Oncology First Affiliated Hospital of ZhengzhouUniversity Zhengzhou Henan 450052 China

Background The effectiveness of chemoradiotherapy followed by surgery （CRTS） in patients with resectable esophageal carcinoma remains *** performed a systematic review of the literature with *** Electronic databases were used to identify published studies between January 1992 and April *** relative risk （RR） with 95％ confidence interval （95％ CI） was utilized to estimate the strength of the association between CRTS and surgery alone （SA） survival of the resectable esophageal carcinoma *** and publication bias were also assessed in the present *** The final analysis of 2755 resectable esophageal carcinoma cases from 21 randomized controlled trials （RCTs） are *** to the SA group,the 1,3-and 5-year survival rates were significantly higher in the CRTS group （all P ＜0.05）; the 3-and 5-year survival rates for the Eastern patients,Western patients,patients undergoing concurrent chemoradiotherapy,patients with squamous cell carcinoma,patients undergoing High-dose radiotherapy （≥40 Gy）,and patients given either ＂cisplatin ＋ Fluorouracil＂ or ＂cisplatin ＋ paclitaxel＂ chemotherapy were significantly higher in the CRTS group （all P ＜0.05）.There were no statistical significances in the 3-and 5-year survival rates for patients undergoing sequential chemoradiotherapy or patients with adenocarcinoma between the two groups （all P ＞0.05）.Compared to the RCTS group,the surgery rate in the SA group was higher （P ＜0.05）,while the CRTS group had significantly higher radical resection rate,R0 resection rate and lower postoperative local recurrence rate （all P ＜0.05）.The differences in postoperative complication incidence,post-operative distant metastasis and postoperative mortality rate were not statistically significant between the two groups （all P ＞0.05）.Conclusion CRTS can significantly improve the survival and surgical conditions of patients with resectable esophageal carcinoma.

关键词： oesophageal carcinoma chemoradiotherapy followed by surgery surgery alone randomized controlled trial meta-analysis

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Clinical application and analysis of hepatitis C virus NS3 antigen detection by EliSA in human serum

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Chinese Medical Journal 2007年第4期120卷 294-299页

作者： XIE li WU Xiao-dong HUANG De-zhuang CheN Hai-lun he li-xiang WANG Jian HAN Da-kang Institute for Viral hepatitis Center for Clinical Laboratory Artificial liver Center Beijing You＇an Hospital Capital Medical University Beijing100069 China Department of Biology Graduate University of Chinese Academyof Sciences Beijing 100049 China Department of Microbiology Capital Medical University Beijing100069 China

Background hepatitis C virus （HCV） core antigen assays have been produced to exclude infectious donations collected during the preseroconversion window phase （PWP）. For the same purpose, we evaluated the specificity and sensitivity of a novel hepatitis C virus NS3 antigen detection immunoassay and the application of this assay in clinical diagnosis. Methods Samples from 77 healthy subjects, 173 anti-HCV positive patients and 3708 hepatitis patients other than HCV positive were tested with the HCV NS3 antigen assay. Some HCV NS3 antigen positive samples were further validated with HCV-RNA, neutralization and immunodot assays. Twenty-five sequential samples from 11 HCV NS3 antigen positive patients were subjected to kinetic study. Results Only 48 （1.3%） of 3708 anti-HCV negative samples were positive for HCV NS3 antigen. Among them, 44 of 3030 samples from patients only infected with HBV were HCV NS3 antigen positive, 4 of the 445 samples from patients infected with other type hepatitis were HCV NS3 antigen positive. In addition, 42 （24.3%） of 173 anti-HCV positive samples were HCV NS3 antigen positive and all 77 samples from healthy subjects were negative to HCV NS3 antigen assay. Of the 15 HCV NS3 antigen positive samples, 9 （60%） were HCV-RNA positive. The neutralization and positive percentage of immunodot assay for 23 HCV NS3 antigen positive sera were 87.0% （20/23） and 69.6% （16/23） respectively. Of the 25 sequential samples from 11 HCV NS3 antigen positive patients, there was a negative correlation between the OD values and the duration of test （r=-0.989, P〈0.05）, and there were correlations among their HCV NS3 antigen, HCV-RNA and anti-HCV Utres. The anti-HCV antibodies of two sera were detected while their OD values of HCV NS3 antigen decreased gradually. Conclusions The HCV NS3 antigen detection assay showed perfect specificity and high sensitivity. Thus, it would be useful and economical as a routine test in laboratories for early diagnosis of HCV inf

关键词： hepatitis C virus hepatitis C virus NS3 antigen hepatitis C virus RNA anti-HCV," EliSA

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Predictive Score Model for Delayed Graft Function Based on Hypothermic Machine Perfusion Variables in Kidney Transplantation

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Chinese Medical Journal 2018年第22期131卷 2651-2657页

作者： Chen-Guang Ding Yang li Xiao-Hui Tian Xiao-Jun Hu Pu-Xun Tian Xiao-Ming Ding he-li xiang Jin Zheng Wu-Jun Xue Department of Kidney Transplantation Nephropathy HospitalThe First Affiliated Hospital of Xi'an Jiaotong UniversityXi'anShaanxi 710061China Institute of Organ Transplantation Xi'an Jiaotong UniversityXi'anShaanxi 710061China

Background: Hypothermic machine perfusion(HMP) is being used more often in cardiac death kidney transplantation; however, the significance of assessing organ quality and predicting delayed graft function(DGF) by HMP parameters is still controversial. Therefore,we used a readily available HMP variable to design a scoring model that can identify the highest risk of DGF and provide the guidance and advice for organ allocation and DCD kidney ***: From September 1, 2012 to August 31, 2016, 366 qualified kidneys were randomly assigned to the development and validation cohorts in a 2:1 distribution. The HMP variables of the development cohort served as candidate univariate predictors for DGF. The independent predictors of DGF were identified by multivariate logistic regression analysis with a P he odds ratios(ORs) value, each HMP variable was assigned a weighted integer, and the sum of the integers indicated the total risk score for each kidney. The validation cohort was used to verify the accuracy and reliability of the scoring ***: HMP duration(OR = 1.165, 95% confidence interval [CI ]: 1.008–1.360, P = 0.043), resistance(OR = 2.190, 95%CI: 1.032–10.20, P he independent predictors of identified DGF. The HMP predictive score ranged from 0 to 14, and there was a clear increase in the incidence of DGF, from the low predictive score group to the very high predictive score group. We formed four increasingly serious risk categories(scores 0–3, 4–7, 8–11, and 12–14)according to the frequency associated with the different risk scores of DGF. The HMP predictive score indicates good discriminative power with a c?statistic of 0.706 in the validation cohort, and it had significantly better prediction value for DGF compared to both terminal flow(P = 0.012) and resistance(P = 0.006).Conclusion: The HMP predictive score is a good noninvasive tool for assessing the qu

关键词： Delayed Graft Function Donation after Cardiac Death Hypothermic Machine Perfusion Kidney Transplantation

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DCD肾移植中DGF高风险受者应用EC-MPS联合低剂量他克莫司治疗的预后分析（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第6期19卷 481-489页

作者： li-zi JIAO Chen-guang DING Pu-xun TIAN Xiao-ming DING Xiao-ming PAN he-li xiang Xiao-hui TIAN Yang li Jin ZheNG Wu-jun XUE Department of Kidney Transplantation the First Affiliated Hospital of Xi'an Jiaotong University Institute of Organ Transplantation Xi'an Jiaotong University

目的:分析心脏死亡供体(DCD)肾移植中移植物功能延迟恢复(DGF)高风险受者应用米芙(EC-MPS)联合低剂量他克莫司治疗术后1年的有效性及安全性,指导临床用药。创新点:对比DGF高风险受者术后发生DGF及正常恢复受者的免疫抑制剂药代动力学特... 详细信息

目的:分析心脏死亡供体(DCD)肾移植中移植物功能延迟恢复(DGF)高风险受者应用米芙(EC-MPS)联合低剂量他克莫司治疗术后1年的有效性及安全性,指导临床用药。创新点:对比DGF高风险受者术后发生DGF及正常恢复受者的免疫抑制剂药代动力学特征。方法:将本中心进行肾移植的61例DGF高风险受者按照实际病情纳入DGF组及正常恢复组,均行米芙联合低剂量他克莫司免疫抑制治疗。对比两组免疫抑制剂血药浓度及预后各项指标。结论:DGF组及正常恢复组间米芙及他克莫司剂量无显著差异。术后1周DGF组EC-MPS血药浓度曲线下面积小于30(mg·h)/L的比率显著高于正常恢复组,同时他克莫司谷浓度显著低于正常恢复组,DGF组经活检证实的急排反应的发生率显著高于正常恢复组,术后1周的EC-MPS血药浓度曲线下面积低于30(mg·h)/L的受者中经活检证实的急排反应的发生率显著高于其他受者。1年移植物存活率、移植物功能及常见不良反应在两组间未见明显差异。

关键词：米芙(EC-MPS) 他克莫司移植物功能延迟恢复(DGF) 心脏死亡供体(DCD) 肾移植

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Early Immunosuppressive Exposure of Enteric-Coated-Mycophenolate Sodium Plus Tacrolimus Associated with Acute Rejection in Expanded Criteria Donor Kidney Transplantation

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Chinese Medical Journal 2018年第11期131卷 1302-1307页

作者： Chen-Guang Ding li-Zi Jiao Feng Han he-li xiang Pu-Xun Tian Xiao-Ming Ding Xiao-Ming Pan Xiao-Hui Tian Yang li Jin Zheng Wu-Jun Xue Department of Renal Transplantation Nephropathy Hospital The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi 710061 China Institute of Organ Transplantation Xi'an Jiaotong University Xi'an Shaanxi 710061 China

Background： lmmunosuppressive agents are still inefficient in preventing biopsy-proven acute rejection （BPAR） alter expanded criteria donor （ECD） kidney transplantation. The aim of this study was to investigate the relationships between early imrnunosuppressive exposure and the development of BPAR. Methods： We performed a retrospective study of 58 recipients of ECD kidney transplantation treated with enteric-coated-mycophenolate sodium, tacrolirnus （Tac）, and prednisone. The levels of mycophenolic acid-area under the curve （MPA-AUC）0-12h and Tac C0 were measured at the 1st week and the 1st month posttransplant, respectively. The correlation was assessed by multivariate logistic regression. Results： The occurrence rates of BPAR and antibody-mediated rejection were 24.1% and 10.3%, respectively. A low level of MPA-AUC0-12h at the 1st week posttransplant was found in BPAR recipients （38.42 ± 8.37 vs. 50.64 ± 13.22, P 〈 0.01）. In addition, the incidence of BPAR was significantly high （P 〈 0.05） when the MPA-AUC0-12h level was 〈30 mg·h-1·L-1 at the 1st week （ 15.0% vs. 44.4%） or the Tac C0 was 〈4 ng/ml at the 1 st month posttransplant （33.3% vs. 21.6%）. Multivariable logistic regression analysis showed that the MPA-AUC 0-12 h at the 1st week （OR： 0.842, 95% CI： 0.784-0.903） and the Tac C0 at the 1st month （OR： 0.904, 95% C7： 0.822-0.986） had significant inverse correlation with BPA R （ P 〈 0.05 ）. Conclusions： Low-level exposure of MPA and Tac C0 in the early weeks posttransplant reflects an increased acute rejection risk, which suggested that MPA-AUC0-12h 〈30 mg·h-1·L -1 and Tac C0 〈4 ng/ml should be avoided in the first few weeks alter transplantation.

关键词： Enteric-Coated-Mycophenolate Sodium Tacrolimus Acute Rejection Expanded Criteria Donor Kidney Transplantation

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基于差分阵列的频控阵雷达距离-角度联合估计

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系统仿真学报 2017年第4期29卷 886-893页

作者：黄玲贺知明李想杨帆兰州理工大学电气工程与信息工程学院甘肃兰州730050 电子科技大学电子工程学院四川成都611731

提出一种频控阵雷达目标距离-角度联合估计的子阵方案,以解决基本频控阵雷达由于距离和方位角耦合而不能直接实现目标距离和方位角二维联合估计的问题。方案将整个阵列分为两个子阵列,且两个子阵列采用不同的频偏。为了扩展阵列孔径,子... 详细信息

提出一种频控阵雷达目标距离-角度联合估计的子阵方案,以解决基本频控阵雷达由于距离和方位角耦合而不能直接实现目标距离和方位角二维联合估计的问题。方案将整个阵列分为两个子阵列,且两个子阵列采用不同的频偏。为了扩展阵列孔径,子阵列均采用差分阵列结构。当采用接收数据的二阶统计量进行处理时,N个实际物理阵元可提供O(N^2)维的自由度。由于两个子阵列对距离和角度维的解耦能力,目标可利用基于子空间的多信号分类算法被定位。目标估计性能是通过分析相对于信噪比的克拉美罗下限来检验的。最后,由数值仿真结果验证了方法的有效性。

关键词：频控阵距离-角度估计差分阵列 Root-Music算法 ESPRIT算法

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考虑铰链间隙的水面并联稳定平台动力学分析

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兵工学报 2017年第1期38卷 129-134页

作者：李豪杰张合李珂翔谢克峰南京理工大学智能弹药技术国防重点学科实验室江苏南京210094 中国船舶重工集团公司第724研究所江苏南京210003

针对某小型浮式稳定平台结构紧凑、负载惯量大的特点,设计了一种以少自由度并联机构为基础的改进型串联与并联机构。根据该平台的结构特点,进行了运动学分析,推导了系统雅可比矩阵,并利用Lagrange方法建立了平台的动力学模型,分析了平... 详细信息

针对某小型浮式稳定平台结构紧凑、负载惯量大的特点,设计了一种以少自由度并联机构为基础的改进型串联与并联机构。根据该平台的结构特点,进行了运动学分析,推导了系统雅可比矩阵,并利用Lagrange方法建立了平台的动力学模型,分析了平台的动力学特性。为分析含间隙的支链受力,将其简化为刚体—弹簧模型,通过改进的hertz接触理论,采用非线性弹簧阻尼模型计算接触力,最终建立了含铰链间隙的平台动力学模型。数值计算与实验结果表明了数据建模的有效性,同时铰链间隙对动平台的位置精度和动力学特性产生了显著影响,对平台结构产生了较强的冲击。

关键词：机械学并联机构稳定平台铰链间隙动力学分析

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