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Early Cretaceous Thrust and Nappe Tectonics in North Qilian Shan,Northern Tibetan Plateau:Evidence from Field Mapping,Geochronology,and Deep Structural Analysis

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Acta Geologica Sinica(English Edition) 2023年第4期97卷 1058-1077页

作者： HAN Lele DING Weicui CheN Xuanhua SHAO Zhaogang zhang Da XIAO Yongjun he Chengguang WANG Zengzhen LI Bing zhang yiping XU Shenglin WANG Ye School of Earth Sciences and Resources China University of Geosciences(Beijing)Beijing 100083China SinoProbe Center Chinese Academy of Geological Sciences and China Geological SurveyBeijing 100037China Exploration and Development Research Institute Shengli Oilfield CompanySinopecDongyingShandong 257000China henan Institute of Geological Survey ZhengzhouHenan 450001China

The North Qilian Shan fold and thrust belt,located at the northern Tibetan Plateau and southern margin of the hexi Corridor,is a key tectonic unit to decode the formation and expansion of the *** studies emphasize the Cenozoic deformation due to the far-field response to the Indo-Asian collision,but the Mesozoic deformations are poorly constrained in this *** conducted detailed field mapping,structural analysis,geochronology,and structural interpretation of deep seismic reflectional profiling and magnetotelluric(MT)sounding,to address the superposed results of the Mesozoic and Cenozoic *** results recognized the North Qilian thrust and nappe system(NQTS),the root and the frontal belt are the North Qilian thrust(NQT),and the Yumu Shan klippe(YK),*** middle belt is located between the NQT and the *** granite zircon U-Pb dating from the middle belt yields an age of ca.415 Ma,which is similar to south *** thrusting displacement is estimated at ca.48 km by structural interpretation of deep *** timing is constrained in the early stage of the Early Cretaceous by the formation of simultaneous growth *** suggest that the NQTS has resulted from the far-field effect of the Lhasa-Qiangtang collision,and the Yumu Shan is uplifted by the superposed Cenozoic deformation.

关键词： geochronology structural analysis seismic reflectional and MT profiling Early Cretaceous North Qilian thrust and nappe system Tibetan Plateau

WIP1 Phosphatase Plays a Critical Neuroprotective Role in Brain Injury Induced by High-Altitude Hypoxic Inflammation

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Neuroscience Bulletin 2017年第3期33卷 292-298页

作者： Dahu Li Lijun zhang Xin Huang Lili Liu yunling he Lun Xu Yiyao zhang Tong Zhao Liying Wu Yongqi Zhao Kuiwu Wu Yan Wu Ming Fan Lingling Zhu Department of Cognitive Science Institute of Basic MedicalSciencesBeijing 100850China Co-innovation Center of Neuroregeneration Nantong UniversityNantong 226001China Beijing Institute for Brain Disorders Beijing 100069China Navy General Hospital of the PLA Beijing 100048China Air Force General Hospital of the PLA Beijing 100142China

The hypobaric hypoxic environment in highaltitude areas often aggravates the severity of inflammation and induces brain injury as a consequence. However, the critical genes regulating this process remain largely unknown. The phosphatase wild-type p53-induced phosphatase 1（WIP1） plays important roles in various physiological and pathological processes, including the regulation of inflammation in normoxia, but its functions in hypoxic inflammation-induced brain injury remain ***, we established a mouse model of this type of injury and found that WIP1 deficiency augmented the release of inflammatory cytokines in the peripheral circulation and brain tissue, increased the numbers of activated microglia/macrophages in the brain, aggravated cerebral histological lesions, and exacerbated the impairment of motor and cognitive abilities. Collectively, these results provide the first in vivo evidence that WIP1 is a critical neuroprotector against hypoxic inflammation-induced brain injury.

关键词： Hypobaric hypoxia Inflammation Brain injury WIP1 phosphatase Lipopolysaccharide

Comparison of spatial-temporal distribution characteristics of water temperatures between Lancang River and Mekong River

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Science Bulletin 2007年第S2期 141-147页

作者： zhang yiping~(1+) GAO Fu~(1,2,4) he DaMing~3 LI ShaoJuan~3 1 Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Kunming 650223,China 2 Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China 3 Asian International Rivers Center,Yunnan University,Kunming 650014,China 4 Graduate University of the Chinese Academy of Sciences,Beijing 100049,China

Water temperature(WT)is one of the important indicators of water body physical ***- standing the characteristics of spatial-temporal variation on WT in the river continuum will help to de- velop the rational river-water-resource utilization plan,to avoid the possible ecology crisis,and to take full advantages of the natural resources in the involved *** the biggest international river con- tinuum in Asia,the Lancang-Mekong river subregion(known as Greater Mekong Subregion,GMS)has become a famous economic,political,and ecological natural *** study examined the spa- tial-temporal distribution characteristics of the Lancang River water temperature,using the observa- tional data of WT values from 11 hydrological stations in ***,the WT differences be- tween Yunjinghong hydrological station(one representative station in China)and Chiang Saen hydro- logical station(one representative station in Thailand)were *** indicated that:1)Water temperature was higher in the lower part of the river continuum than the upper *** variation is the main influencing *** effect is more obvious in dry season than in rainy season,more obvious in winter than in summer,and more obvious of the average minimum WT than the average maximum WT;2)WT difference between the upper and the lower parts of the river continuum was de- creasing due to little increase of WT in the upper part(Yunjinghong hydrological station)and obvious decrease of the WT in the lower part(Chiang Saen hydrological station)during the research period. Therefore,the parameters of WT are affected by the latitude,and the Lancang-Mekong river continuum lies in the north-south direction,so the tropical aquatic creatures,such as the tropical aquatic fishes, cannot survive in the upper part of this river continuum in winter season,while in summer season,the tropical aquatic creatures properly swim to the upper *** water temperature in the lower Me- kong R

关键词： Lancang-Mekong river river channel water temperature trends of variation Longitudinal Range-Gorge Region

铜藻多酚的提取工艺及其稳定性和抑制酪氨酸酶活性

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食品研究与开发 2024年第9期45卷 104-110页

作者：何袅袅蔡树芸阎光宇杨婷陈伟珠洪专张怡张怡评自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心福建厦门361005 福建农林大学食品科学学院福建福州350001 厦门海洋职业技术学院海洋生物学院福建厦门361100

对铜藻多酚提取工艺、稳定性及其对酪氨酸酶活性的抑制作用进行研究。以新鲜铜藻为原料提取铜藻多酚,以铜藻多酚提取率为指标,考察乙醇体积分数、提取时间、液料比对铜藻多酚提取率的影响,并根据Box-Behnken试验设计对铜藻多酚的提取条... 详细信息

对铜藻多酚提取工艺、稳定性及其对酪氨酸酶活性的抑制作用进行研究。以新鲜铜藻为原料提取铜藻多酚,以铜藻多酚提取率为指标,考察乙醇体积分数、提取时间、液料比对铜藻多酚提取率的影响,并根据Box-Behnken试验设计对铜藻多酚的提取条件进行优化,得到铜藻多酚的最佳提取条件:乙醇体积分数40%、超声时间53 min、液料比为60∶1(mL/g),在该条件下所制备的铜藻多酚具有较高的热和光照稳定性。以左旋酪氨酸和左旋多巴为底物,铜藻多酚浓度为700μg/mL时,对酪氨酸酶活性的抑制率分别为92.68%和39.18%。

关键词：铜藻多酚提取工艺稳定性酪氨酸酶

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壳源氦气成藏主控因素及资源评价方法研究

地学前缘 2024年第1期31卷 340-350页

作者：吴义平王青陶士振王建君李谦张宁宁吴晓智李浩武王晓波中国石油勘探开发研究院北京100083

当前国内外尚无系统的氦气资源评价方法以及针对性的参数取值标准。通过天然气和氦气的成藏要素对比,明确了载体气和壳源氦气的异源同储、同源同储和同源异储3类共生关系,以及壳源氦气成藏的8个关键因素。构建了4类10种氦气资源分级分... 详细信息

当前国内外尚无系统的氦气资源评价方法以及针对性的参数取值标准。通过天然气和氦气的成藏要素对比,明确了载体气和壳源氦气的异源同储、同源同储和同源异储3类共生关系,以及壳源氦气成藏的8个关键因素。构建了4类10种氦气资源分级分类评价方法,其中含量法包括5个亚类,统计法包括1个亚类,类比法包括3个亚类,成因法1个亚类,初步解决了氦气资源定量计算的难题。结果表明:高氦气含量的气藏一般为常压及低压气藏,氦气含量与氦源岩类型、铀钍含量和氦源岩规模3个参数呈正向关系,而与离主断裂距离、埋深和生烃强度3个参数呈负相关,适度的基底构造活动有利于氦气释放及富集。基于8类氦气成藏主控因素与氦气含量的定量关系,建立了氦气含量类比法。基于异源同储不同序的原理,构建了氦气资源规模序列法;基于放射性元素衰变释氦机理,建立了氦气成因法。氦气资源评价方法在国内外得到了较为广泛的应用,研究成果将为我国氦气储量规模发现提供了有效支撑。

关键词：载体气共生关系氦气含量生氦法规模序列资源评价

A 3D Ni_(8)-cluster-based MOF as a molecular electrocatalyst for alcohol oxidation in alkaline media

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Chinese Journal of Structural Chemistry 2023年第2期42卷 5-8页

作者： Wei-Juan Chen Tian-Yu zhang Xue-Qian Wu Yong-Shuang Li yunling Liu Ya-Pan Wu Zhao-Bo he Dong-Sheng Li College of Materials and Chemical Engineering Key Laboratory of Inorganic Nonmetallic Crystalline and Energy Conversion MaterialsChina Three Gorges UniversityYichang443002China College of Electrical Engineering&New Energy China Three Gorges UniversityYichang443002China Hubei Three Gorges Laboratory Yichang443007China State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of ChemistryJilin UniversityChangchun130012China

*** DAFCs offer significant promise for compact electric devices through the consumption of liquid fuels as one of the renewable energy sources,with minimal environmental effects and prominent energy density[1-4].Beca... 详细信息

关键词： alcohol alkaline cluster

清肾颗粒通过miR-4516/SIAH3/PINK1调节线粒体自噬而减轻大鼠肾纤维化

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中国病理生理杂志 2024年第8期40卷 1479-1487页

作者：任克军金华王东茅燕萍戴荣程梦王亿平张磊安徽中医药大学第一附属医院安徽合肥230031

目的:明确清肾颗粒(QSG)通过微小RNA-4516(miR-4516)/SIAH3/PINK1调节线粒体自噬而减轻大鼠肾纤维化的作用机制。方法:将雄性SD大鼠随机分为3组,包括正常组、模型组和QSG组,每组10只,予QSG水溶液灌胃,每天1次,每次4 mL,连续给药8周。检... 详细信息

目的:明确清肾颗粒(QSG)通过微小RNA-4516(miR-4516)/SIAH3/PINK1调节线粒体自噬而减轻大鼠肾纤维化的作用机制。方法:将雄性SD大鼠随机分为3组,包括正常组、模型组和QSG组,每组10只,予QSG水溶液灌胃,每天1次,每次4 mL,连续给药8周。检测各组大鼠血肌酐水平;苏木精-伊红(he)和Masson染色检测各组大鼠肾脏病理损伤程度;Werstern blot检测各组大鼠肾脏组织中β-actin、PINK1、Parkin、SIAH3、VDAC1、Mfn1、Mfn2、OPA1、LC3B和P62蛋白表达水平;RT-qPCR检测大鼠肾脏组织中SIAH3的mRNA表达水平;透射电镜观察肾脏组织中线粒体损伤情况。制备QSG含药血清,转化生长因子β1(TGF-β1)诱导HK-2细胞纤维化模型,细胞分为:正常对照(NC)组、模型(MC)组、MC+miR-4516 mimics组、MC+miR-4516 NC+QSG组、MC+miR-4516 mimics+QSG组和MC+QSG组。CCK-8法检测各组细胞活力;Western blot检测各组细胞中E-cadherin和α-SMA蛋白表达水平;双萤光素酶报告基因实验验证miR-4516对SIAH3的调节;RT-qPCR检测miR-4516、SIAH3 mRNA和PINK1 mRNA的表达。结果:与模型组比较,QSG干预后大鼠肾组织及HK-2细胞纤维化减轻,SIAH3的mRNA表达水平显著降低(P1表达水平显著升高(P16的表达水平,抑制SIAH3的mRNA表达进而促进HK-2细胞中PINK1表达,降低纤维化标志蛋白α-SMA蛋白的表达水平。结论:QSG可通过干预miR-4516水平而靶向调节SIAH3/PINK1轴,增强线粒体自噬,从而延缓大鼠肾纤维化的进展。

关键词：清肾颗粒微小RNA-4516 SIAH3/PINK1轴线粒体自噬肾纤维化

清肾颗粒通过调控外泌体、miR-330-3p以及CREBBP表达抑制小鼠肾纤维化

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南方医科大学学报 2024年第8期44卷 1431-1440页

作者：戴荣曹泽平刘传娇葛永程梦王伟丽陈义珍张磊王亿平安徽中医药大学第一附属医院肾内科安徽合肥230031 安徽中医药大学第一临床医学院安徽合肥230038

目的探讨清肾颗粒对腺嘌呤诱导的肾纤维化小鼠模型及尿酸刺激下NRK-49F细胞的作用及其调控外泌体、miR-330-3p和CREBBP的机制。方法动物实验:构建腺嘌呤诱导的肾纤维化小鼠模型,分为正常组、模型组和清肾颗粒组(8.0 g·kg^(-1)·d^(-1)... 详细信息

目的探讨清肾颗粒对腺嘌呤诱导的肾纤维化小鼠模型及尿酸刺激下NRK-49F细胞的作用及其调控外泌体、miR-330-3p和CREBBP的机制。方法动物实验:构建腺嘌呤诱导的肾纤维化小鼠模型,分为正常组、模型组和清肾颗粒组(8.0 g·kg^(-1)·d^(-1)),6只/组,灌胃12周。采用尾静脉注射腺相关病毒载体,实验结束后收集双侧肾组织。观察小鼠肾组织外泌体标记蛋白CD9,Hsp70,TSG101蛋白水平变化;Western blotting及免疫荧光检测小鼠肾组织Col-III、α-SMA、FN和E-cad表达变化;he和Masson染色观察小鼠肾组织病理学变化。细胞实验:构建尿酸刺激下的NRK-49F细胞模型(尿酸400μmol/L),SD大鼠灌胃制备清肾颗粒含药血清用于细胞实验,分为正常组、模型组和清肾颗粒含药血清组。观察NRK-49F细胞中外泌体标记蛋白CD9,Hsp70,TSG101蛋白水平变化;Western blotting及免疫荧光检测NRK-49F细胞中Col-III、α-SMA、FN和E-cad表达变化;双荧光素酶报告基因检测miR-330-3p与CREBBP靶向关系;RT-qPCR和Western blotting检测NRK-49F细胞中miR-330-3p与CREBBP表达变化。结果动物实验中,Western blotting结果显示,与正常组相比,模型组CD9、Hsp70、TSG101蛋白水平升高(P101蛋白水平降低(P<0.05)。Western blotting及免疫荧光结果显示,与正常组相比,模型组Col-III、α-SMA、FN表达增加,E-cad表达减少(P<0.05);与模型组相比,清肾颗粒组Col-III、α-SMA、FN表达减少,E-cad表达增加(P<0.05)。肾脏病理检查显示,清肾颗粒可以明显减轻小鼠肾组织纤维化(P<0.05)。RT-qPCR结果显示,尾静脉注射腺相关病毒病毒载体抑制miR-330-3p表达,增加CREBBP水平,减轻肾纤维化(P<0.05)。双荧光素酶报告基因结果显示,CREBBP是miR-330-3p的靶点(P<0.05)。RT-qPCR和Western blotting结果显示,与正常组相比,模型组miR-330-3p表达增加,CREBBP表达减少(P<0.05);与模型组相比,清肾颗粒组miR-330-3p表达减少,CREBBP表达增加(P<0.05)。细胞实验结果与动物实验结果趋势一致。结论清肾颗粒通过干预外泌体,降低miR-330-3p水平,增加CREBBP表达抑制肾纤维化。

关键词：清肾颗粒外泌体 miR-330-3p 肾纤维化

Xeno-repopulation of Fah^(-/-)Nod/Scid mice livers by human hepatocytes

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Science China(Life Sciences) 2011年第3期54卷 227-234页

作者： SU BaoLiang LIU ChangCheng XIANG Dao zhang HaiBin YUAN SiMing WANG MinJun CheN Fei ZHU HaiYing he ZhiYing WANG Xin HU yiping Department of Cell Biology Second Military Medical University Shanghai 200433 China Center for Instrumental Analysis China Pharmaceutical University Nanjing 210009 China Eastern hepatobiliary Surgery Hospital Second Military Medical University Shanghai 200433 China Department of Plastic Surgery Nanjing General Hospital of Nanjing Military Command Nanjing 210002 China Institute of Biochemistry and Cell Biology Shanghai Institute for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Functional human hepatocytes xenografted into the liver of mice can be used as a model system to study pharmacokinetics,infection of hepatitis viruses,and the efficacy of hepatitis *** levels of liver xeno-repopulation have been reported in Fah-/-Rag2-/-Il2rg-/-***,the high mortality and low breeding rate of this model may hinder its application.A new model,termed Fah-/-Nod/Scid mice,which combines the advantages of liver repopulation in Fah-/-mice with the ease of xenotransplantation in Nod/Scid mice was obtained by gradual ***-/-Nod/Scid mice were easily maintained in breeding colonies and in adult animal care ***506 treatment combined with gradual withdrawal of NTBC before cell transplantation ensured that Fah-/-Nod/Scid mice were susceptible to liver xeno-repopulation by human hepatocytes;the proportion of engrafted human hepatocytes reached 33.6%.The function of the expanded human hepatocytes within the chimeric liver was confirmed by weight curve analysis,the expression of characteristic proteins,and the biochemical analysis of liver *** results show that Fah-/-Nod/Scid mice are an ideal humanized liver mouse model with many useful applications.

关键词： human hepatocyte humanized liver cell transplantation Fah gene knockout mice Nod/Scid mice