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Chinese Journal of Electronics 2024年

作者： Yuhang QIU Gongming ZHAO Hongli XU Long LI he huang Liusheng huang School of Computer Science and Technology University of Science and Technology of China Suzhou Institute for Advanced Research University of Science and Technology of China School of Computer Science and Technology Soochow University

The virtual private cloud service currently lacks a real-time end-to-end consistency validation mechanism, which prevents tenants from receiving immediate feedback on their requests. Existing solutions consume excessive communication and computational resources in such large-scale cloud environments, and suffer from poor timeliness. To address these issues, we propose a lightweight consistency validation mechanism that includes real-time incremental validation and periodic full-scale validation. The former leverages message layer aggregation to enable tenants to swiftly determine the success of their requests on hosts with minimal communication overhead. The latter utilizes lightweight validation checksums to compare the expected and actual states of hosts locally, while efficiently managing the checksums of various host entries using inverted indexing. This approach enables us to efficiently validate the complete local configurations within the limited memory of hosts. In summary, our proposed mechanism achieves closed-loop implementation for new requests and ensures their long-term effectiveness.

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An Intelligent Bridge Multi-Dimension Deflection IoT Monitoring System Based on Laser Datum and Imaging

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Journal of Traffic and Transportation Engineering(English Edition) 2024年

作者： Dan Zhao Huifeng Wang Hao Du Haoyi Cheng Lu Peng Yaru Liang he huang Yunmeng Li School of Electronic &Control Engineering Chang'an University Research Institute of Highway Ministry of Transport State Key Laboratory of Traction Power Southwest Jiaotong University

Aiming at the all-weather automatic monitoring of bridge structure health monitoring， an embedded bridge multi-dimensional (horizontal and vertical) deflection monitoring system based on dual laser references is proposed. The controller turns on the laser reference transmitter through instructions to emit the laser reference. The intelligent target system located in the middle of the span will calculate the multi-dimensional deflection and deformation information of the bridge structure according to the laser spot projected on the diffuse optical target， and pass this information through the wireless network communication unit transmits to the cloud platform to realize real-time monitoring of the bridge structure status and other parameters. Through the laboratory platform， it is found that the measurement error of this method is less than 300 μm， and the data update rate is less than 66 ms， which meets the requirements of bridge structural health monitoring. This method solves the problem of low efficiency of artificial bridge deflection monitoring， realizes all-weather online real-time monitoring of bridge deflection detection， and ensures the accuracy and efficiency of the measurement system.

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Establishment of a humanized mouse model using steady-state peripheral blood-derived hematopoietic stem and progenitor cells facilitates screening of cancer-targeted T-cell repertoires

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Cancer Innovation 2024年第3期3卷 1-21页

作者： Yulin Xu Wei Shan Qian Luo Meng Zhang Dawei Huo Yijin Chen Honghu Li Yishan Ye Xiaohong Yu Yi Luo he huang Bone Marrow Transplantation Center The First Affiliated HospitalZhejiang University School of MedicineHangzhouChina Liangzhu Laboratory Zhejiang UniversityHangzhouChina Institute of hematology Zhejiang UniversityHangzhouChina Zhejiang Province Engineering Research Center for Stem Cell and Immunity Therapy HangzhouChina School of Medicine Zhejiang UniversityHangzhouChina

Background:Cancer-targeted T-cell receptor T(TCR-T)cells hold promise in treating cancers such as hematological malignancies and breast cancers.However,approaches to obtain cancer-reactive TCR-T cells have been unsuccessful.Methods:here,we developed a novel strategy to screen for cancer-targeted TCR-T cells using a special humanized mouse model with person-specific immune fingerprints.Rare steady-state circulating hematopoietic stem and progenitor cells were expanded via three-dimensional culture of steady-state peripheral blood mononuclear cells,and then the expanded cells were applied to establish humanized mice.The human immune system was evaluated according to the kinetics of dendritic cells,monocytes,T-cell subsets,and cytokines.To fully stimulate the immune response and to obtain B-cell precursor NAML-6-and triple-negative breast cancer MDA-MB-231-targeted TCR-T cells,we used the inactivated cells above to treat humanized mice twice a day every 7 days.Then,human T cells were processed for TCRβ-chain(TRB)sequencing analysis.After the repertoires had been constructed,features such as the fraction,diversity,and immune signature were investigated.Results:The results demonstrated an increase in diversity and clonality of T cells after treatment.The preferential usage and features of TRBV,TRBJ,and the V–J combination were also changed.The stress also induced highly clonal Science and Technology,Grant/Award Number:2021C03010;Zhejiang Provincial Natural Science Foundation of China,Grant/Award Numbers:LTGY24H080003,LY21H080004 expansion.Tumor burden and survival analysis demonstrated that stress induction could significantly inhibit the growth of subsequently transfused live tumor cells and prolong the survival of the humanized mice.Conclusions:We constructed a personalized humanized mouse model to screen cancer-targeted TCR-T pools.Our platform provides an effective source of cancer-targeted TCR-T cells and allows for the design of patient-specific engineered T cells.It therefore has the potential to greatly benefi

关键词： cancer-targeted T-cell receptor T(TCR-T)cells circulating hematopoietic stem and progenitor cells(HSPCs) humanized mouse model steady-state peripheral blood T-cell receptorβ-chain(TRB) three-dimensional culture

Installing hydrogen bonds as a general strategy to control viscosity sensitivity of molecular rotor fluorophores Special Collection:Aggregation-Induced Processes and Functions

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Aggregate

Aggregate 2024年第1期5卷 367-376页

作者： Baoxing Shen Lihua Liu Yubo huang Jichun Wu Huan Feng Yu Liu he huang Xin Zhang Department of Chemistry Research Center for Industries of the FutureWestlake UniversityHangzhouChina Westlake Laboratory of Life Sciences and Biomedicine HangzhouChina School of Food Science and Pharmaceutical Engineering Nanjing Normal UniversityNanjingChina CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina

Molecular rotor-based fluorophores(RBFs)activate fluorescence upon increase of micro-viscosity,thus bearing a broad application promise in many fields.However,it remains a challenge to control how fluorescence of RBFs responds to viscosity changes.herein,we demonstrate that the formation and regulation of intramolecular hydrogen bonds in the excited state of RBFs could modulate their rotational barrier,leading to a rational control of how their fluorescence can be activated by micro-viscosity.Based on this strategy,a series of RBFs were developed based on 4-hydroxybenzylidene-imidazolinone(HBI)that span a wide range of viscosity sensitivity.Combined with the AggTag method that we previously reported,the varying viscosity sensitivity and emission spectra of these probes enabled a dualcolor imaging strategy that detects both protein oligomers and aggregates during the multistep aggregation process of proteins in live cells.In summary,our work indicates that installing intracellular excited state hydrogen bonds to RBFs allows for a rational control of rotational barrier,thus allow for a fine tune of their viscosity sensitivity.Beyond RBFs,we envision similar strategies can be applied to control the fluorogenic behavior of a large group of fluorophores whose emission is dependent on excited state rotational motion,including aggregation-induced emission fluorophores.

关键词： fluorescent probe intramolecular hydrogen bond protein aggregation rotor-based fluorophores viscosity sensitivity

Aptamer-Based Cell-Surface Profiling with Single-Cell Resolution Enables Precise Cancer Characterization

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CCS Chemistry 2024年第1期6卷 196-207页

作者： Liujun Xu Yawei Feng Tong Wang Shenhuan Li Kangli Xu Yue Sun Yi Luo Yishan Ye Yan Miao Yun Dong Zhenzhen Guo Qing Zhang Benshang Li he huang Xue-Qiang Wang Liping Qiu Weihong Tan Molecular Science and Biomedicine Laboratory State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringCollege of BiologyAptamer Engineering Center of Hunan ProvinceHunan UniversityChangshaHunan 410082 The Key Laboratory of Zhejiang Province for Aptamers and Theranostics Zhejiang Cancer HospitalHangzhou Institute of MedicineChinese Academy of SciencesHangzhouZhejiang 310022 Institute of Molecular Medicine Renji HospitalShanghai Jiao Tong University School of MedicineCollege of Chemistry and Chemical EngineeringShanghai Jiao Tong UniversityShanghai 200240 Bone Marrow Transplantation Center The First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003 Institute of hematology Zhejiang UniversityHangzhou 310003 Department of hematology and Oncology Shanghai Children’s Medical CenterShanghai Jiao Tong University School of MedicineShanghai Jiao Tong UniversityShanghai 200127

Molecular profiling of cell-surface proteins is a powerful strategy for precise cancer diagnosis.While mass cytometry(MC)enables synchronous detection of over 40 cellular parameters,its full potential in disease classification is challenged by the limited types of recognition probes currently available.In this work,we synthesize a panel of heavy isotopeconjugated aptamers to profile cancer-associated signatures on the surface of hematological malignancy(HM)cells.Based on 15 molecular signatures,we performed cell-surface profiling that allowed the precise classification of 8 HM cell lines.Combined with machine-learning technology,this aptamer-based MC platform also achieved multiclass identification of HM subtypes in clinical sampleswith 100%accuracy in the training cohort and 80%accuracy in the test cohort.Therefore,we report an effective and practical strategy for precise cancer classification at the singlecell level,paving the way for its clinical use in the near future.

关键词： molecular profiling cancer diagnosis mass cytometry aptamers machine learning

Coconut oil research:Past,present,and future directions

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Food and health 2024年第1期6卷 43-52页

作者： Jiao-Jiao Yin he huang Xiao-Ming Jiang Xing-Yang Guo Bao-Hui Pan Pan Gao Wu Zhong Chuan-Rong Hu Dong-Ping he School of Food Science and Engineering Wuhan Polytechnic UniversityWuhan 430023China Key Laboratory of Deep Processing of Major Grain and Oil Ministry of EducationWuhan 430023China Hubei Key Laboratory for processing and Transformation of Agricultural Products Wuhan 430023China Key Laboratory of Edible Oil Quality and Safety for State Market Regulation Wuhan 430023China Wuhan Institute for Food and Cosmetic Control Wuhan 430071China

Coconut oil contains a rich amount of medium-chain fatty acids,including lauric acid,decanoic acid,and octanoic acid,as well as the corresponding medium-chain triglycerides.It possesses functional attributes such as facile digestion and absorption,as well as antibacterial and antioxidant properties.The study utilized the Citespace and VOSviewer visual analysis software to examine the quantity of published papers,authors,publishing institutions,research hotspots and frontiers of 3442 effective literatures on the theme of"coconut oil"in the Web of Science(WoS)Core Collection database.The research on coconut oil can be condensed into four primary sections:(1)investigation and utilization of physiochemical characteristics of coconut oil,(2)analysis of nutritional composition and study of the effectiveness of coconut oil,(3)identification of adulteration in coconut oil,(4)evaluation of the impact of coconut oil as a dietary supplement on animal metabolism.Additionally,research focal points have evolved in three distinct phases.Prior to 2008,numerous studies were conducted to investigate the precise fatty acid makeup of coconut oil and its potential for lowering cholesterol levels.From 2009 to 2016,significant emphasis was placed on examining the impact of coconut oil on methane production in ruminants.Between 2018 to 2023,the main focus will be on investigating how nanoparticles can alter the properties of coconut oil.In the future,the anticipated research areas of interest are expected to focus on the rapid detection method of coconut oil,the efficacy of coconut oil and the advancement of coconut resources.The objective of this review is to provide researchers with relevant information about coconut oil,aiming to foster the continued growth of the coconut oil industry.

关键词： coconut oil visualized analysis research hotspots physiochemical properties nutrient composition

Review on synergistic damage effect of irradiation and corrosion on reactor structural alloys

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Nuclear Science and Techniques 2024年第3期35卷 109-141页

作者： Hui Liu Guan-Hong Lei he-Fei huang Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China School of Nuclear Science and Technology University of Chinese Academy of SciencesBeijing 100049China

The synergistic damage effect of irradiation and corrosion of reactor structural materials has been a prominent research focus.This paper provides a comprehensive review of the synergistic effects on the third-and fourth-generation fission nuclear energy structural materials used in pressurized water reactors and molten salt reactors.The competitive mechanisms of multiple influencing factors,such as the irradiation dose,corrosion type,and environmental temperature,are summarized in this paper.Conceptual approaches are proposed to alleviate the synergistic damage caused by irradiation and corrosion,thereby promoting in-depth research in the future and solving this key challenge for the structural materials used in reactors.

关键词： Irradiation and corrosion Synergistic effect Austenitic stainless steels Nickel-based alloys Reactors

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Malvidin Mitigates Sepsis-induced Cardiac Injury by Modulating the TLR4-iNOS-COX-2 Inflammatory Pathway and the Bax/Bcl-2/Cyto-C Mitochondrial Apoptosis Pathway in a p38 MAPK-dependent Manner

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Biomedical and Environmental Sciences 2024年第2期37卷 221-227页

作者： ZHANG Wei YUAN Si Long QIANG Jing Chao huang he LI Da SUN Ying ZHANG Hong Gang Department of Vascular Surgery The First People’s Hospital of LianyungangLianyungang 222005JiangsuChina Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening College of PharmacyJiangsu Ocean UniversityLianyungang 222005JiangsuChina Jinzhou Medical University postgraduate training base(Lianyungang First People’s Hospital) Lianyungang 222005JiangsuChina

In critical care medicine,sepsis is a dangerous systemic condition that is highly prevalent and is associated with high morbidity and mortality rates^([1]).The high mortality rate associated with sepsis is closely related to multi-organ dysfunction,with heart injury being particularly critical and considered the starting point of multi-organ injury^([2]).

关键词： mortality Apoptosis TLR4

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Inpatient disease spectrum and clinical characteristics of surgical disorders in pediatric and adolescent gynecology: A single-institute study of 12 years data from China

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中华医学杂志(英文版) 2024年第10期137卷 1240-1242页

作者： huang he Wang Siyun Zhan Ruixi Yin Ling Hu Yongchun Yang Donglai Shi Yangyang Department of Obstetrics and Gynecology Peking University First Hospital Xicheng District Beijing China Department of Obstetrics and Gynecology Inner Mongolia Ulanqab Second Hospital Ulanqab Inner Mongolia China Department of Obstetrics and Gynecology Jiujiang University Affiliated Hospital Jiujiang Jiangxi China

Pediatric and adolescent gynecology (PAG) is a specialized branch of gynecology that focuses on reproductive health issues of women from infancy to adolescence.[1] In 1939, the Hungarian Dobszay first established the new branch. Since then, the PAG subject has begun to develop and gradually matured in the past 80 years. While PAG has witnessed significant development in western countries, a substantial unmet need persists in addressing specific health concerns among children and adolescents with gynecological disorders. In China, where the PAG discipline is still in its infancy, this situation becomes even more critical. The diseases young females experience in the gynecological field have own particularities.[ 1] Also, PAG shares connections with various medical disciplines, notably surgery. Investigating the inpatient disease spectrum, prevalence rates, and clinical characteristics of PAG surgical disorders is of significant clinical importance, yet no previous research has addressed this in China or other countries. Therefore, the study retrospectively analyzed the clinical data of 512 PAG inpatients aged 0-18 years who received surgical treatment at Peking University First Hospital (PKUFH) from January 2010 to May 2022, so as to comprehensively outline the inpatient disease spectrum and clinical characteristics of surgical disorders in PAG, with the aim of offering management recommendations and evidential support for future clinical work. The study was approved by the Institutional Review Board of PKUFH (No. 2022[593]) and informed consent was waived due to the retrospective nature of the study. Non-normally distributed quantitative variables are shown as median (Q1, Q3), and categorical variables as count and percentage. Comparison tests were performed using chi-squared test and Fisher's exact test.

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A novel method of utilizing static mixer to obtain mixing homogeneity of multi-species powders in laser metal deposition

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Chinese Journal of Aeronautics 2023年第1期36卷 423-433页

作者： Jipeng CheN Shouchun XIE he huang School of Mechanical and Electronic Engineering Nanjing Forestry UniversityNanjing 210037China Nanjing Huirui Photoelectric Technology Co. LtdNanjing 211121China

Real-time mixing of multi-species powder challenges Laser Metal Deposition(LMD)of Functionally Graded Materials(FGMs).The current work proposes a novel method of using a static mixer to realize rapid,uniform multi-species powder mixing.Firstly,copper powder and 316L stainless steel powder are selected to complete the powder mixing observation experiment with Scanning Electron Microscope(SEM)and Energy Dispersive Spectrometer(EDS).Secondly,computational fluid dynamics and particle mixing simulation models are used to analyze the flow field and particle motion characteristics in the static mixer.Finally,LMD experiment and metallo-graphic observation are carried out with 316L stainless steel powder and WC powder to verify the feasibility of the static mixer.This study provides a theoretical and practical basis for powder mixing in laser processing with a static mixer.The conclusions can also be applied to other processing fields requiring real-time and uniform mixing of multi-species powders.

关键词： Homogeneity Laser metal deposition Multi-species powder Mixing Static mixer

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