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Occurrence of K1 and K2 serotypes and genotypic characteristics of extended spectrumβ-lactamases-producing Klebsiella pneumoniae isolated from selected hospitals in Malaysia

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Asian Pacific Journal of Tropical Medicine 2024年第1期17卷 30-38页

作者： Nurul Syazrah Anuar hazmin hazman Sharven Raj Jeyakumar Mohd Nasir Mohd Desa Hasni Idayu Saidi Siti Norbaya Masri Nur Afiza Aziz Nurshahira Sulaiman Department of Biomedical Science Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400UPM SerdangSelangorMalaysia Department of Medical Microbiology Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400UPM SerdangSelangorMalaysia Microbiology Unit Department of PathologyHospital Sultanah AminahJalan Persiaran Sultan Abu Bakar80100 Johor BahruJohorMalaysia

Objective:To determine the distribution,phenotypic and genetic background of extended spectrumβ-lactamases(ESBL)-producing Klebsiella(K.)pneumoniae clinical isolates associated with K1 and K2 serotypes in two selected hospitals in ***:A total of 192 *** isolates were collected and subjected to antibiotic susceptibility,hypermucoviscosity test and multiplex PCR to detect the presence of K1-and K2-serotype associated *** sequence typing(MLST)was performed on ESBL-producing *** isolates presented with K1 and K2 serotypes,followed by phylogenetic ***:A total of 87 out of 192(45.3%)of the *** isolates collected were ESBL ***,only 8.3%(16/192)and 10.9%(21/192)of the total isolates were detected to carry K1-and K2-serotype associated genes,*** analysis showed that K1 and K2 capsular serotypes were not significantly associated with ESBL phenotype(P=0.196).However,they were significantly associated with hypervirulent,as demonstrated by the positive string test(P<0.001).MLST analysis revealed that ST23 as the predominant sequence type(ST)in the K1 serotype,while the ST in the K2 serotype is more ***:Although the occurrence of ESBL-producing isolates among the hypervirulent strains was low,their coexistence warrants the need for continuous *** showed that these isolates were genetically heterogeneous.

关键词： Extended spectrumβ-lactamases Klebsiella pneumoniae Capsular serotypes Genotypic

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Enhanced IDS with Deep Learning for IoT-Based Smart Cities Security

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Tsinghua Science and Technology 2024年第4期29卷 929-947页

作者： Chaimae hazman Azidine Guezzaz Said Benkirane Mourade Azrour Higher School of Technology Essaouira Cadi Ayyad UniversityMarrakesh 81000Morocco Faculty of Sciences and Technologies Moulay Ismail UniversityErrachidia 52000Morocco

Cyberattacks against highly integrated Internet of Things (IoT) servers, apps, and telecoms infrastructure are rapidly increasing when issues produced by IoT networks go unnoticed for an extended period. IoT interface attacks must be evaluated in real-time for effective safety and security measures. This study implements a smart intrusion detection system (IDS) designed for IoT threats, and interoperability with IoT connectivity standards is offered by the identity solution. An IDS is a common type of network security technology that has recently received increasing interest in the research community. The system has already piqued the curiosity of scientific and industrial communities to identify intrusions. Several IDSs based on machine learning (ML) and deep learning (DL) have been proposed. This study introduces IDS-SIoDL, a novel IDS for IoT-based smart cities that integrates long shortterm memory (LSTM) and feature engineering. This model is tested using tensor processing unit (TPU) on the enhanced BoT-IoT, Edge-IIoT, and NSL-KDD datasets. Compared with current IDSs, the obtained results provide good assessment features, such as accuracy, recall, and precision, with approximately 0.9990 recording time and calculating times of approximately 600 and 6 ms for training and classification, respectively.

关键词： intrusion detection LSTM IoT security ML DL TPU

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全基因组关联分析(GWAS)鉴定水稻氮素利用效率候选基因

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中国水稻科学 2024年第5期38卷 516-524页

作者：吕阳刘聪聪杨龙波曹兴岚王月影童毅 Mohamed hazman 钱前商连光郭龙彪沈阳农业大学水稻研究所沈阳110161 中国水稻研究所水稻生物育种全国重点实验室杭州311401 中国农业科学院农业基因组研究所深圳518120 埃及农业研究中心农业遗传工程研究所埃及吉萨12588 尼罗河大学生物技术学院埃及谢赫·扎耶德12588 (深圳)生物育种技术有限公司深圳518120

【目的】挖掘水稻氮高效的种质和基因资源,揭示其分子机制和遗传效应,是当前水稻氮素利用效率(NUE)研究的重要内容和目标。【方法】为了鉴定与水稻NUE相关的变异位点和候选基因,我们收集了190份亚洲稻为关联群体,通过质量过滤和群体频... 详细信息

【目的】挖掘水稻氮高效的种质和基因资源,揭示其分子机制和遗传效应,是当前水稻氮素利用效率(NUE)研究的重要内容和目标。【方法】为了鉴定与水稻NUE相关的变异位点和候选基因,我们收集了190份亚洲稻为关联群体,通过质量过滤和群体频率过滤筛选出3,934,195个高质量的单核苷酸多态性(SNPs)位点,在大田条件下设置低氮(N1,90kg/hm2)和常规氮(N2,180kg/hm2)水平,成熟期调查水稻剑叶叶宽在低氮和常规氮处理下的表型数据,结合FarmCPU和MLM模型进行全基因组关联分析(GWAS)。【结果】通过植株在不同氮水平下的叶宽表型数据,计算该群体在低氮和常规氮水平下的剑叶宽表型比值Q(N1/N2),Q值呈现正态分布的特征。对Q值进行全基因组关联分析,在12条染色体上共鉴定了100个显著位点,确定了39个候选QTLs,包括已克隆NUE相关基因OsNR1.2和OsNAC42。进一步鉴定了候选基因OsNR1.2和OsNAC42的优异单倍型和潜在的优势单倍型组合,为水稻NUE的改良提供了有价值的资源和信息。【结论】利用GWAS和单倍型分析,揭示了水稻剑叶叶宽在不同氮处理下的遗传基础,鉴定了与NUE相关的候选QTLs和基因,包括OsNR1.2和OsNAC42。通过组合单倍型分析,鉴定了两个基因的优势单倍型组合,为水稻NUE的改良提供了有价值的资源和信息。

关键词：水稻氮素利用效率叶宽全基因组关联分析

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