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Traditional medicines and their in-vitro proof against Staphylococcus aureus in Pakistan

Asian Pacific Journal of Tropical Medicine 2018年第6期11卷 355-368页

作者： Muhammad Adnan Akash Tariq Roqaia Bibi Sakina Mussarat Bibi Fatima Nawab Ali hazir rahman Zabta Khan Shinwari Department of Botany Kohat University of Science and TechnologyKohat 26000Pakistan Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041 University of Chinese Academy of Sciences Beijing 100039China Department of Biotechnology and Genetic Engineering Kohat University of Science and TechnologyKohat 26000Pakistan Department of Microbiology Abdul Wali Khan UniversityMardan 23200Pakistan Department of Biotechnology and Bioinformatics Quaid-i-Azam UniversityIslamabad 45320Pakistan

Objective:To gather the fragmented literature on ethnobotany,phytochemistry and in-vitro activities of medicinal plants of Pakistan being used against common infections caused by Staphylococcus aureus(***).Methods:A large number of published and unpublished research studies related to the ethnomedicinal,phytochemical and *** activity of medicinal flora of Pakistan published from 1990-2018 were reviewed using online bibliographic databases such as PubMed,Web of Science,Science Direct,ResearchGate and ***:*** can cause many human ailments including endocarditis,staphylococcal scalded skin syndrome,septic arthritis,respiratory problems with an estimated infection rate of 25%-35%across the *** review comprised of 86 medicinal *** showed that people mostly used leaves(50%)for the preparation of traditional *** analysis on the reviewed data revealed that methanolic extract concentrations of medicinal plants was highly significantly positive correlated(r=0.8;P<0.01)with the *** zone of *** reportedly showed complete resistant to the commonly used antibiotic *** compounds like altheahexacosanyl lactone,cinnamaldehyde,niloticane,gobicusin A,asparacosin A,muzanzagenin,isoagatharesinol,friedelin,inophynone and eugenol were active against *** study provided in-vitro proof for the flora of Pakistan used against different infections caused by ***:Antibacterial agents from natural sources could be more effective against bacterial pathogens and will be helpful in minimizing the adverse effects of synthetic drugs,and hence provides a base for the pharmaceutical industries.

关键词： Gram positive bacteria Herbal medicines Plant extracts Phytochemicals In-vitro

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First report on molecular characterization of Leishmania species from cutaneous leishmaniasis patients in southern Khyber Pakhtunkhwa province of Pakistan

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Asian Pacific Journal of Tropical Medicine 2017年第7期10卷 785-788页

作者： Mubbashir Hussain Shahzad Munir Sultan Ayaz Bahar Ullah Khattak Taj Ali Khan Niaz Muhammad Muhammad Anees hazir rahman Muhammad Qasim Muhammad Ameen Jamal Irfan Ahmed Kashif Rahim Humaira Mazhar Noha Watanay Mohamed Kasbari Vector Borne Diseases Laboratory Department of MicrobiologyKohat University of Science and Technology Kohat Department of Plant Pathology Faculty of Plant ProtectionYunnan Agricultural University College of Animal Husbandry and Veterinary Sciences Abdul Wali Khan University Mardan College of Animal Science and Technology Yunnan Agricultural University Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and BiotechnologyCollege of Life SciencesBeijing Normal University US Naval Medical Research Unit-3 (NAMRU 3) ANSES Animal Health Laboratory

Objective: To report presence of Leishmania major in Khyber Pakhtunkhwa of Pakistan, where cutaneous leishmaniasis(CL) is endemic and was thought to be caused by Leishmania tropica only. Methods: Biopsy samples from 432 CL suspected patients were collected from 3 southern districts of Khyber Pakhtunkhwa during years 2011–2016. Microscopy on Giemsa stained slides were done followed by amplification of the ribosomal internal transcribed spacer 1 gene. Results: Leishmania amastigotes were detected by microscopy in 308 of 432 samples(71.3%) while 374 out of 432 samples(86.6%) were positive by ribosomal internal transcribed spacer 1 PCR. Subsequent restriction fragment length polymorphism confirmed Leishmania tropica in 351 and Leishmania major in 6 biopsy samples. Conclusions: This study is the first molecular characterization of Leishmania species in southern Khyber Pakhtunkhwa. It confirmed the previous assumptions that anthroponotic CL is the major CL form present in Khyber Pakhtunkhwa province. Furthermore, this is the first report of Leishmania major from a classical anthroponotic CL endemic focus identified in rural areas of Kohat district in southern Khyber Pakhtunkhwa.

关键词： Cutaneous leishmaniasis ITS-PCR RFLP Leishmania tropica Khyber Pakhtunkhwa

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