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Synthesis of an eco-friendly nanocomposite fertilizer for common bean based on carbon nanoparticles from agricultural waste biochar

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Pedosphere 2021年第6期31卷 923-933页

作者： dina M.SALAMA Mahmoud E.abd el-AZIZ Mehrez E.el-NAGGAR Essam A.SHAABAN Moham.d S.abd el-WAHEd Vegetable Research department National Research Centre33 El Bohouth StreetDokkiGiza 12622(Egypt) Polymers&Pigments department National Research Centre33 El Bohouth StreetDokkiGiza 12622(Egypt) textile Research division National Research Centre33 El Bohouth StreetDokkiGiza 12622(Egypt) Pomology department National Research Centre33 El Bohouth StreetDokkiGiza 12622(Egypt) Botany department National Research Centre33 El Bohouth StreetDokkiGiza 12622(Egypt)

the burning of agricultural waste is a major cause of environmental *** this study,we sought to prepare biochar from agricultural waste as a source material for the preparation of carbon nanoparticles(CNPs).Surface morphology,hydrodynamic particle size,and purity and crystallinity of CNPs were extensively investigated using transmission electron microscopy(tEM),zeta sizing,and X-ray diffraction(XRd)spectroscopy,*** CNPs were subsequently immersed in a solution of potassium nitrate(KNO_(3))to prepare a CNPs/NK nanocomposite(CNPs loaded with nitrogen(N)and potassium(K))as a nanocomposite fertilizer for common bean(Phaseolus vulgaris L.).the CNPs/NK nanocomposite was sprayed as a foliar fertilizer at 0,10,20,30,and 40 mg L^(-1) on common bean plants 25 d after sowing on a farm in Shebin el-Kom,el-Monifia,*** growth,yield,and quality of common bean were investigated during two successive growing seasons(2017 and 2018).the highest seed yields of 2.04 and 2.01 t ha^(-1) and the highest values of growth parameters including plant height of 61.5 and 59.2 cm,number of leaves per plant of 35 and 35,number of flowers per plant of 83.3 and 82.7,and plant fresh weight of 148.7 and 152.8 g plant^(-1) were obtained when using the CNPs/NK nanocomposite at a concentration of 20 mg L^(-1) during the 2017 and 2018 growing seasons,respectively.

关键词： foliar fertilizer N and K-loaded nanosized carbon particles Phaseolus vulgaris L. plant growth yield

Hypoglycemic and antioxidant activities of Caesalpinia ferrea Martius leaf extract in streptozotocininduced diabetic rats

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Asian Pacific Journal of tropical Biomedicine 2015年第6期5卷 460-470页

作者： Sherien Kamal Hassan Nermin Mohammed el-Sammad Amria Mamdouh Mousa Maha Hashim Mohammed abd el Razik Hussein Farrag Amani Nassir eldin Hashim Victoria Werner Ulrike Lindequist Mahmoud abd el-Moein Nawwar departement of Biochemistry National Research CentreCairo Egypt departement of Pathology National Research CentreCairo Egypt departement of Phytochemistry and Plant Systematics National Research CentreCairo Egypt Institute of Pharmacy Pharmaceutical Biology Ernst-Moritz-Arndt-Universitt GreifswaldD-17487 Greifswald Germany

Objective: to evaluate the antidiabetic and antioxidant ef ects of aqueous ethanolic extract of Caesalpinia ferrea(C. ferrea) leaf in normal and streptozotocin(StZ) induced diabetic ***: Male Sprague-dawley rats divided into 6 groups of 6 rats each were assigned into diabetic and non-diabetic groups. diabetes was induced in rats by single intraperitoneal administration of StZ(65 mg/kg body weight). C. ferrea extract at the doses of 250 and 500 mg/kg body weight was orally administered to both diabetic and non-diabetic animals for a period of 30 days. After completion of experimental duration serum, liver and pancreas were used for evaluating biochemical and histopathological ***: Oral administration of C. ferrea leaf extract significantly reduced elevated serum glucose, α-amylase, liver function levels and signii cantly increased serum insulin, total protein and body weight as well as improved lipid proi le due to diabetes. Furthermore, the treatment resulted in a marked increase in glutathione peroxidase, superoxide dismutase, catalase and reduced glutathione, and diminished levels of lipid peroxidation in liver and pancreas of diabetic rats. Histopathological studies demonstrated the reduction in the pancreas and liver damage and coni rmed the biochemical i ***: From the present study, it can be concluded that the C. ferrea leaf extract ef ectively improved hyperglycaemia while inhibiting the progression of oxidative stress in StZ-induced diabetic rats. Hence, it can be used in the management of diabetes mellitus.

关键词： Caesalpinia ferrea diabetes Streptozotocin Hypoglycemia Antioxidant markers Histopathology

trends and outcomes of pancreaticoduodenectomy for periampullary tumors: A 25-year single-center study of 1000 consecutive cases

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World Journal of Gastroenterology 2017年第38期23卷 7025-7036页

作者： ayman el nakeeb waleed askar ehab atef ehab el hanafy ahmad m sultan tarek salah ahmed shehta moham.d el sorogy emad hamdy moham.d el hemly ahmed a el-geidi tharwat kandil moham.d el shobari talaat abd allah amgad fouad mostafa abu zeid ahmed abu el eneen nabil gad el-hak gamal el ebidy omar fathy ahmed sultan moham.d abdel wahab gastroenterology surgical center mansoura university

AIM to evaluate the evolution, trends in surgical approaches a n d r e c o n s t r u c t i o n t e c h n i q u e s, a n d i m p o r t a n t lessons learned from performing 1000 consecutive pancreaticoduodenectomies(Pds) for periampullary *** this is a retrospective review of the data of all patients who underwent Pd for periampullary tumor during the period from January 1993 to April 2017. the data were categorized into three periods, including early period(1993-2002), middle period(2003-2012), and late period(2013-2017).RESULtS the frequency showed Pd was increasingly performed after the year 2000. With time, elderly, cirrhotic and obese patients, as well as patients with uncinate process carcinoma and borderline tumor were increasingly selected for Pd. the median operative time and postoperative hospital stay decreased significantly over the periods. Hospital mortality declined significantly, from 6.6% to 3.1%. Postoperative complications significantly decreased, from 40% to 27.9%. there was significant decrease in postoperative pancreatic fistula in the second 10 years, from 15% to 12.7%. there was a significant improvement in median survival and overall survival among the *** Surgical results of Pd significantly improved, with mortality rate nearly reaching 3%. Pancreatic reconstruction following Pd is still debatable. the survival rate was also improved but the rate of recurrence is still high, at 36.9%.

关键词： Pancreaticoduodenectomy Pancreaticogastrostomy Pancreaticojejunostomy Postoperative pancreatic fistula Periampullary tumor

Nephroprotective Effect of <i>Nigella Sativa</i>and <i>Matricaria Chamomilla</i>in Cisplatin Induced Renal Injury—Supportive treatments in Cisplatin Nephrotoxicity

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International Journal of Clinical Medicine 2011年第3期2卷 185-195页

作者： Ragaa H. M Salama Nahed A abd-el-hameed Sary KH abd-el-Ghaffar Zaghloul t. Mohammed Nagwa M. A Ghandour department of Medical Biochemistry

Nigella sativa and Matricaria chamomilla are extensively consumed as tea or tonic. despite their widespread use as a home remedy, relatively few trials evaluated their benefits in nephroprotection. Hence, this study evaluates the nephroprotective effects of supportive treatments (N. sativa, M. chamomilla and vitamin E) in cisplatin nephrotoxicity rat model. Eighty rats divided into 10 groups, of 8 animals each. the first group (G1) injected with saline intra-pretoneal (i.p). G2 injected with 5 mg/kg cisplatin i.p on zero day of experiment and repeated 4 times, with 5 days free interval. G3-G10 received daily supportive treatments, started 5 days before the experiment (–5day). Concomitantly G4, G6, G8 and G10 injected with 5 mg/kg cisplatin i.p like G2. On day sixteen, animal scarified, serum and/or kidney tissue were used to determine kidney function tests (serum urea, creatinine, NAG, β-gal), oxidative stress indices (NO, LPO), antioxidant activities (SOd), sulphur compounds (GGt, GSH, total thiols ), apoptotic indices (cathepsin d, dNA fragmentation), two minerals (Ca2+ and zn2+). Cisplatin caused marked elevation in serum GGt that reduced signifi-cantly in group received M. chamomilla with cisplatin (P there is a correlation between GGt and NAG in cisplatin group (r = 0.731 p that may suggest one of possible mechanisms of renal injury by cisplatin. M. chamomilla followed by N. sativa and vitamin E improved the biochemical and pathological renal injury, as determined by increasing the body weight, normalizing the kidney functions, decreasing the oxidative stress markers, improving the apoptotic markers, minimizing the pathological changes. Hence, N. sativa and M. chamomilla will be a promising nephroprotective agents for reducing cisplatin nephrotoxicity, most probably, by antioxidants effects and inhibition GGt production, respectively.

关键词： Cisplatin Nephrotoxicity Nephroprotection Matricaria Chamomilla Nigella Sativa Vitamin E

Analysis and design of UHF Bow-tie RFId tag Antenna Input Impedance

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Open Journal of Antennas and Propagation 2016年第2期4卷 85-107页

作者： d. A. abd el-Aziz t. G. Abouelnaga E. A. abdallah M. el-Said Yaser S. E. abdo National Institute for Standards Giza Egypt electronics Research Institute Giza Egypt Faculty of Engineering Cairo University Giza Egypt

In this paper two proposed methods of input impedance calculation for Bow-tie antenna are introduced. the proposed methods show input impedance calculation with high accuracy. Also, design curves for input impedance values were developed depending on the geometry of antenna. the proposed design curves are used to design a Bow-tie type RFId tag antenna. the input impedance of the tag antenna is calculated using proposed methods and compared with that obtained using CSt studio suite 2014 and IE3d Zeland version 12.0 software packages. the results are investigated and discussed. the tag antenna is fabricated, measured and the obtained input impedance is compared with the simulation and the proposed methods. Good agreement among measured input impedance and that simulated by CSt, IE3d or proposed methods is obtained.

关键词： Bandwidth Bow-tie Antenna Radio Frequency Identification (RFId) Ultra High Frequency (UHF)

HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro

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World Journal of Gastroenterology 2006年第30期12卷 4836-4842页

作者： Mostafa K el-Awady Ashraf A tabll Yasmine S el-abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader el din el-Rashdy M Redwan Maha el-demellawy Moataza H Omran Wael t el-Garf Said A Goueli department of Biomedical technology National Research Center Tahrir Street PO 12622 Dokki Cairo Egypt department of Medicinal Chemistry Na- tional Research Center Tahrir Street PO 12622 Dokki Cairo Egypt department of Microbiology and Immunology Faculty of Pharmacy Cairo University Cairo Egypt Genetic Engineering and Biotechnology Research Institute New Borg El-Arab City Alexandria Egypt department of Pathology School of Medicine University of Wisconsin Madison WI United States

AIM： tO establish a cell culture system with longterm replication of hepatitis C virus （HCV） genome and expression of viral antigens in vitro. MEtHOdS： HepG2 cell line was tested for its susceptibility to HCV by incubation with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various time points during the culture. Culture supernatant was tested for its ability to infect na＇ive cells. the presence of minus （antisense） RNA strand, and the detection of core and E1 antigens in cells were examined by Rt-PCR and immunological techniques （flow cytometry and Western blot） respectively. RESULtS： the intracellular HCV RNA was first detected on d 3 after infection and then could be consistently detected in both cells and supernatant over a period of at least three months. the fresh cells could be infected with supernatant from cultured infected cells. Flow cytometric analysis showed surface and intracellular HCV antigen expression using in house made polyclonal antibodies （anti-core, and anti-E1）. Western blot analysis showed the expression of a cluster of immunogenic peptides at molecular weights extended between 31 and 45 kda in an one month old culture of infected cells whereas this cluster was undetectable in uninfected HepG2 cells. CONCLUSION： HepG2 cell line is not only susceptible to HCV infection but also supports its replication in vitro. Expression of HCV structural proteins can be detected in infected HepG2 cells. these cells are also capable of shedding viral particles into culture media which in turn become infectious to uninfected cells.

关键词： Hepatitis C virus In vitro propagation Genomic replication Gene expression HepG2 cells

A dosimetric comparison between 3d-Conformal radiation therapy and intensity modulated radiation therapy plans in the treatment of posterior fossa boost in children with high risk medulloblastom

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the Chinese-German Journal of Clinical Oncology 2013年第11期12卷 540-545页

作者： Saad el din I abd el AAl H Makaar W Mashhour K el Beih d Hashem W department of Clinical Oncology and Radiotherapy Kasr Al-Ainy HospitalCairo UniversityCairo Egypt Physics Section in department of Clinical Oncology and Radiotherapy Kasr Al-Ainy Hospital Cairo UniversityCairo Egypt

Objective： the work is a comparative study between two modalities of radiation therapy, the aim of which is to compare 3d conformal radiation therapy （3d-CRt） and intensity modulated radiation therapy （IMRt） in treating posterior fossa boost in children with high risk medulloblastorna; dosimetrically evaluating and comparing both techniques as regard target coverage and doses to organs at risk （OAR）. Methods： twenty patients with high risk medulloblastoma were treated by 3d-CRt technique. A dosimetric comparison was done by performing two plans for the posterior fossa boost, 3d-CRt and IMRt plans, for the same patient using Eclipse planning system （version 8.6）. Results： IMRt had a better conformity index compared to 3d-CRt plans （P value of 0.000）. As for the dose homogeneity it was also better in the IMRt plans, yet it hasn＇t reached the statistical significant value. Also, doses received by the cochleae, brainstem and spinal cord were significantly less in the IMRt plans than those of 3d-CRt （P value 〈 0.05）. Conclusion： IMRt technique was clearly able to improve conformity and homogeneity index, spare the cochleae, reduce dose to the brainstem and spinal cord in comparison to 3d- CRt technique.

关键词： 3d-conformal dosimetric IMRt medulloblastoma cochleae

A highly sensitive bio-barcode immunoassay for multi-residue detection of organophosphate pesticides based on fluorescence antiquenching

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Journal of Pharmaceutical Analysis 2022年第4期12卷 637-644页

作者： Lingyuan Xu Xiuyuan Zhang A.M.abd el-Aty Yuanshang Wang Zhen Cao Huiyan Jia J.-Pablo Salvador Ahmet Hacimuftuoglu Xueyan Cui Yudan Zhang Kun Wang Yongxin She Fen Jin Lufei Zheng Baima Pujia Jing Wang Maojun Jin Bruce d.hammock Institute of Quality Standard and testing technology for Agro-Products Chinese Academy of Agricultural SciencesBeijing100081China department of Pharmacology Faculty of Veterinary MedicineCairo UniversityGiza12211Egypt department of Medical Pharmacology Medical FacultyAtaturk UniversityErzurum25240Türkiye Institute of Livestock and Poultry Ningbo Academy of Agricultural SciencesNingboZhejiang315040China Nanobiotechnology for diagnostics Group Instituto de Química Avanzada de CatalunaIQAC-CSICBarcelona08034Spain CIBER de Bioingeniería Biomateriales y Nanomedicina(CIBER-BBN)Madrid28029Spain Inspection and testing Center of Agricultural and Livestock Products of tibet Department of Agriculture and Rural Affairs of Tibet Autonomous RegionLhasa850000China department of Entomology&Nematology and the UC davis Comprehensive Cancer Center University of CaliforniaDavisCA95616USA

Balancing the risks and benefits of organophosphate pesticides(OPs)on human and environmental health relies partly on their accurate measurement.A highly sensitive fluorescence anti-quenching multi-residue bio-barcode immunoassay was developed to detect OPs(triazophos,parathion,and chlorpyrifos)in apples,turnips,cabbages,and *** nanoparticles were functionalized with monoclonal antibodies against the tested *** oligonucleotides were complementarily hybridized with an RNA fluorescent label for signal *** detection signals were generated by dNA-RNA hybridization and ribonuclease H dissociation of the *** resulting fluorescence signal enables multiplexed quantification of triazophos,parathion,and chlorpyrifos residues over the concentration range of 0.01-25,0.01-50,and 0.1-50 ng/mL with limits of detection of 0.014,0.011,and 0.126 ng/mL,*** mean recovery ranged between 80.3% and 110.8% with relative standard deviations of 7.3%-17.6%,which correlate well with results obtained by liquid chromatography-tandem mass spectrometry(LC-MS/MS).the proposed bio-barcode immunoassay is stable,reproducible and reliable,and is able to detect low residual levels of multi-residue OPs in agricultural products.

关键词： Organophosphate pesticides Fluorescence anti-quenching Gold nanoparticles dNA-RNA hybridization dNA oligonucleotides Ribonuclease H

Synthesis of Pyrroles and Condensed Pyrroles as Anti-Inflammatory Agents with Multiple Activities and their Molecular docking Study

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Open Journal of Medicinal Chemistry 2015年第4期5卷 49-96页

作者： M. t. Sarg M. M. Koraa A. H. Bayoumi S. M. abd el Gilil department of Organic Chemistry Faculty of Pharmacy-Girls Al Azhar University Cairo Egypt department of Organic Chemistry Faculty of Pharmacy Cairo University Giza Egypt department of Organic Chemistry Faculty of Pharmacy-Boys Al Azhar University Cairo Egypt

We herein disclose a series of novel pyrrole derivatives as well as fused pyrrolopyridines 6a,b and 7a,b, pyrrolopyrazoles 8a, b, pyrrolo[2,3-d]pyrimidine derivatives 10a-d, 12a,b, 14a,b, 18a,b, 20a,b, 21a,b, 22a,b, 23a,b, 24a,b, 31a,b, 36a,b, 40a,b, pyrrolo[1,2,6]thiadiazine derivatives 19a,b, pyrrolotriazolopyrimidines 25a,b, 26a,b, 27a,b and 28a,b, pyrrolo[2,3-d][1,2,3]triazine derivatives 32a,b and pyrrolo[2,3-d][1,3]oxazine derivatives 39a,b as novel compounds. All compounds were evaluated for their anti-inflammatory, analgesic (compared to the reference drug Indomethacin) and antimicrobial activities (compared to the reference drug Ampicillin and Fluconazole). Compounds 4d, 5b-d, 6a,b, 9c,d, 10d, 12ab, 13b, 19a,b, 21b, 23b, 31a,b, 38b and 40a were found to be the most active anti-inflammatory drugs exhibiting potency ranging from 1 - 1.01 compared to the reference drug indomethacin. In addition to docking study of these highly active twenty compounds against the active site of cyclooxygenase-2 enzyme (COX-2), among the tested compounds, compounds 5d, 9d, 11b, 12a, 13b and 32a showed multiple activities;anti-inflammatory, analgesic and anti-bacterial activities.

关键词： Anti-Inflammatory Activity Pyrrole Pyrrolo[2 3-d]Pyrimidine Molecular Modeling