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Distribution of Candida Species and Molecular Typing of C. albicans Isolates in a Mexico City Tertiary Care Hospital from 2011 to 2013

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Open Journal of Medical Microbiology 2016年第2期6卷 66-79页

作者： haydee torres guerrero Israel Martínez Espinosa Misael Gonzales Ibarra Magdalena Aguirre García Unidad de Medicina Experimental Facultad de Medicina Universidad Nacional Autónoma de México México D.F. México Laboratorio de Inmunoalergología y Micología Médica División de Investigación Hospital Juárez de México México D.F. México

The incidence of yeast infections has increased significantly over the past few years, constituting a leading cause of morbidity and mortality among hospitalised patients. The rapid identification of candidiasis is important for the clinical management of patients and to facilitate tracing the sources of infections in hospitalized patients. Here, we report a retrospective, single-centre study of Candida spp. distribution and antifungal susceptibility from January 2011 to May 2013 at a hospital in México City, regarding the importance of elucidating the identity of the infection-causing Candida species in order to improve prophylactic measures and treatment. Clinical data were collected from patient medical records and the laboratory database. Isolates were initially identified using standard mycology techniques, and then confirmed by PCR-based system using amplification of intergenic spacers (rDNA ITS) and restriction length polymorphism of PCR products after sequence-specific enzymatic cleavage (PCR-RFLP). We observed no shift from C. albicans to non-albicans Candida species: Candida albicans (73.7%) was the most prevalent species isolated, while C. dubliniensis was not identified in this study. Antifungal susceptibility was determined using FUNGITEST®17.4% of C. albicans isolates were resistant to fluconazole and 21.7% to itraconazole. Multiplex PCR microsatellite analysis of the clinical C. albicans isolates using primers for the CAI, CAIII and CAVI loci identified 29 different alleles for CAI, 8 alleles for CAIII and 31 for CAVI. The combined discriminatory power of these three microsatellites was 0.98, which was considered reliable for molecular typing. Genetic analysis of these isolates revealed a clonal population with a total of 62 genotypes from the examined isolates

关键词： Candidiasis rDNA-ITS RFLP Microsatellites Molecular Typing CAI Candida spp.

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Microevolution of <i>Candida albicans</i>Isolate from a Patient with Mucocutaneous Candidiasis and HIV Infection

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Open Journal of Medical Microbiology 2017年第2期7卷 41-49页

作者： Gabriel Palma Cortés Carlos Cabello Gutierrez Misael González Ibarra Magdalena Aguirre García Fernando Hernández Sánchez haydee torres guerrero Departamento de Investigación en Virología y Micología Instituto Nacional de Enfermedades Respiratoria “Ismael Cosío Villegas” Ciudad de México México Laboratorio de Inmuno Alergología y Micología Médica División de Investigación Hospital Juárez de México Ciudad de México México Departamento de Medicina Experimental Facultad de Medicina Universidad Nacional Autónoma de México Hospital General de México “Dr. Eduardo Liceaga” Ciudad de México México

Candidiasis is the most common opportunistic fungal infection in HIV patients, and its presence is ascribed mainly to the persistence of the original infecting strain. The latter might acquire genetic variations during interaction with the host, reflecting the adaptation of the strain. Here, we report the case of a 32-year-old man complaining of asthenia, irregular hyperpyrexia, and dry cough, who was admitted to the emergency unit. Laboratory examination showed positivity for HIV. Dark violet macular lesions and ulcerated lesions with verrucous erosion were observed at the tip of the nose, whereas an ulcer without exudates was noted in the pubic region. Candida albicans was recovered from the skin by scraping these lesions. Cultures from the bronchoalveolar lavage (BAL) were negative for bacteria and opportunistic fungi but were positive for Candida albicans. The isolates from the skin and BAL were typed by PCR-RFLP and Candida albicans was identified. Analysis by microsatellite length polymorphisms, established that the pubic isolate was a genetic variant of the isolate from the nose and mouth. This suggested a microevolutionary event. Despite clinical support, the patient died of multiple organ failure.

关键词： Candida albicans HIV PCR-RFLP Microsatellite Genotyping Microevolution

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Synchronization of Robot Manipulators Actuated By Induction Motors with Velocity Estimator

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Computer Modeling in Engineering & Sciences 2019年第11期121卷 609-630页

作者： Felipe J.torres Gerardo V.guerrero Carlos D.García Ricardo Zavala-Yoe Mario A.García Adolfo R.López University of Guanajuato Division of Engineering Campus Irapuato-SalamancaCarretera Salamanca-Valle de Santiago km 3.5+1.8GuanajuatoSalamanca36885México National Technological of Mexico/National Center of Research and Technological Development Av.Palmira S/N colonia PalmiraMorelosCuernavaca62490México School of Engineering and Sciences Monterrey TechnologicalPuente 222 Ejidos de HuipulcoCiudad de México14380México Superior Technological Institute of Irapuato Carretera Irapuato-Silao km 12.5GuanajuatoIrapuato36821México

A complete modeling(including the actuator dynamics)of a robot manipulator that uses three-phase induction motors is presented in this paper.A control scheme is designed to synchronize robot manipulators actuated by induction motors under a masterslave scheme in the case where the joint velocity of the slave robots is *** of the research on the synchronization of robot manipulators assumes the use of ideal actuators to drive the joints;for that reason,in this work,a three-phase induction motor is considered to be a direct-drive actuator for each *** entire model of the mated system is obtained by a combination of the dynamics of the induction motor and robot ***,the synchronization control algorithm for a master-slave scheme in both the joint space and workspace of robot manipulators driven by induction motors is *** observer based on the entire model is proposed to estimate the joint velocity of the slave robot *** the Lyapunov criterion,a stability analysis of the synchronization control with a velocity estimator is *** analytical results show the synchronization and estimation errors are globally,uniformly,and ultimately *** with multiple robots demonstrate the performance of the proposed control algorithm.

关键词： Synchronization robot manipulator induction motor Lyapunov stability observer based on the model

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The Ways of Thinking in a Sample of the Student Community of the Subjects of Structured Programming and Numerical Methods in Engineering of the UAM-Azcapotzalco within the Emerging Remote Teaching Project (PEER)

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International Journal of Intelligence Science 2022年第3期12卷 39-56页

作者： Lourdes Sánchez-guerrero Ana Lilia Laureano-Cruces Martha Mora-torres Javier Ramírez-Rodríguez Departamento de Sistemas Universidad Autónoma Metropolitana-Azcapotzalco Mexico City Mexico Posgrado en Diseñ o y Visualización de la Información Universidad Autónoma Metropolitana-Azcapotzalco Mexico City Mexico

Due to the pandemic that is currently being experienced worldwide, educational institutions (HEIs) have had to reinvent and innovate the mode of teaching, in order to continue the process of training students at all educational levels. Higher education has not been immune to this situation, the institutions of this educational level face various challenges in remote teaching: one of them, perhaps the most important, was to answer the question: How to get the student to continue their undergraduate studies remotely? In this sense, it was necessary, on the part of the students and the teachers, a preparation to go from the face-to-face to the virtual mode. This paper addresses the specific case of the analysis of the teaching-learning process of the Teaching-Learning Unit (UEA for its acronym in Spanish) of Structured Programming and Numerical Methods for Engineering at the undergraduate level, at the Universidad Autónoma Metropolitana, Unidad Azcapotzalco (UAM-A) and its relation to the student’s way of thinking. Likewise, the analysis will be carried out if the way of thinking of the students of the sample influences their academic performance when studying the subject of Structured Programming and Numerical Methods for Engineering.

关键词： Intelligent Teaching Systems Emerging Remote Teaching Project (PEER) Way of Thinking Cognition Motivation and the Locus of Control

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Brassinosteroids and Plant Responses to Heavy Metal Stress. An Overview

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Open Journal of Metal 2013年第2期3卷 34-41页

作者： Miriam Núnez Vázquez Yanelis Reyes guerrero Lisbel Martínez González Walfredo torres de la Noval Department of Plant Physiology and Biochemistry National Institute of Agricultural SciencesSan Joséde las LajasCuba

Soil contamination with heavy metals has become a world-wide problem, leading to the loss in agricultural productivity. Plants have a remarkable ability to take up and accumulate heavy metals from their external environment and it is well known that high levels of heavy metals affect different physiological and metabolic processes. Brassinosteroids are considered as the sixth class of plant hormones and they are essential for plant growth and development. These compounds are able of inducing abiotic stress tolerance in plants. In this paper, information about brassinosteroids and plant responses to heavy metal stress is reviewed.

关键词： Heavy Metal Stress Brassinosteroids Tolerance

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Combining Ability and Heterosis in Corn Breeding Lines to Forage and Grain

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American Journal of Plant Sciences 2014年第6期5卷 845-856页

作者： Cesar guerrero guerrero Miguel A. Gallegos Robles José Guadalupe Luna Ortega Ignacio Orona Castillo Cirilo Vázquez Vázquez Mario García Carrillo Alejandro Moreno Resendez Anselmo González torres Catederatico de la Universidad Politécnica de la Región Laguna México Catederaticos de la UAAAAUL Torreon México Direccion General de Educación Tecnológica Industrial Torreón México Universidad Juárez del Estado de Durango Venecia Dgo México

This study was conducted in two stages, in 2006 the formation of cross, in the experimental field of the Universidad Autonoma Agraria Antonio Narro Unidad Laguna (UAAANUL), and the evaluation of the same in 2007;at two locations with three environments, a locality was the experimental field of UAAANUL during spring and summer cycles and another location was the Niagara, Municipality of Aguascalientes, during the spring cycle. The genetic material used consisted of three groups of lines: the first group formed by four lines from the UAAANUL, the second group, two lines from INIFAP, and the third group, 10 lines of the International Center for Maize Improvement and Wheat (CIMMYT). The main objective was to estimate the effects of general combining ability (GCA) of the lines, and the specific combining ability (SCA) of their crosses and heterosis, to estimate its use in a breeding program. Estimates of general combining ability (GCA) and specific combining ability (SCA) were obtained using an analysis of line x line. To forage yield (FY), B-40 (16.84 t·ha-1) and AN-447 (1.54 t·ha-1) and to grain yield (GY), AN-388R (2.31 t·ha-1) had the greatest effect of GCA in both types of yield. The greatest effects of SCA for (FY) were cross AN-447XCML-264 (17.56 t·ha-1) and AN-388RXCML-319 (16.54 t·ha-1), in (GY) were cross AN-447XCML-315 (1.94 t·ha-1) and B-40XCML-319 (1.78 t·ha-1). For heterosis, higher value in forage yield was cross AN-447XCML-264 (20.7%), in grain yield, were B-40XCML-319 (27.1%) and B-32XCML-319 (22.1%) crosses. These results show that it is possible to structure a hybrid program using best lines from CIMMYT, INIFAP and UAAANUL.

关键词： Zea mays L. Hybrids Genetic Material Crosses SCA

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Gut microbiota in a population highly affected by obesity and type 2 diabetes and susceptibility to COVID-19

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World Journal of Gastroenterology 2021年第41期27卷 7065-7079页

作者： Jaime García-Mena Karina Corona-Cervantes Daniel Cuervo-Zanatta Tizziani Benitez-guerrero JuanManuel Vélez-Ixta Norma Gabriela Zavala-torres Loan Edel Villalobos-Flores Fernando Hernández-Quiroz Claudia Perez-Cruz Selvasankar Murugesan Fernando Guadalupe Bastida-González Paola Berenice Zárate-Segura Departamento de Genética y Biología Molecular Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalMexico City 07360CDMXMexico Departamento de Genética y Biología Molecular and Departamento de Farmacología Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalMexico City 07360CDMXMexico Departamento de Farmacología Centro de Investigación y de Estudios Avanzados del Instituto Politécnico NacionalMexico City 07360CDMXMexico Department of Mother and Child Health SIDRA MedicineDoha 26999Qatar Laboratorio Estatal de Salud Pública del Estado de México ISEMToluca de Lerdo 50180Estado de MéxicoMexico Escuela Superior de Medicina Instituto Politécnico NacionalMexico City 11340CDMXMexico

Coronavirus disease 2019(COVID-19)is a disease produced by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and it is currently causing a catastrophic pandemic affecting humans *** disease has been lethal for approximately 3.12 million people around the world since January ***,among the most affected countries,Mexico ranks third in deaths after the United States of America and *** the high number of deceased people might also be explained by social aspects and lifestyle customs in Mexico,there is a relationship between this high proportion of deaths and comorbidities such as high blood pressure(HBP),type 2 diabetes,obesity,and metabolic *** official epidemiological figures reported by the Mexican government have indicated that 18.4%of the population suffers from HBP,close to 10.3%of adults suffer from type 2 diabetes,and approximately 36.1%of the population suffers from *** in the gut microbiota(GM)have been associated with these diseases and with COVID-19 severity,presumably due to inflammatory *** data about the association between GM dysbiosis and metabolic diseases could suggest that the high levels of susceptibility to SARSCoV-2 infection and COVID-19 morbidity in the Mexican population are primarily due to the prevalence of type 2 diabetes,obesity,and metabolic syndrome.

关键词： SARS-CoV-2 COVID-19 High blood pressure Hypertension Type 2 diabetes Obesity Metabolic syndrome Gut microbiota Immunity

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An Affective-Motivational Interface for a Pedagogical Agent

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International Journal of Intelligence Science 2014年第1期4卷 17-23页

作者： Martha Mora-torres Ana Lilia Laureano-Cruces Fernando Gamboa-Rodríguez Javier Ramírez-Rodríguez Lourdes Sánchez-guerrero CCADET Universidad Nacional Autónoma de México México City México Departamento de Sistemas Universidad Autónoma Metropolitana-Azcapotzalco México City México Laboratoire Informatique d’Avignon Université d’Avignon et des Pays de Vaucluse Avignon France Posgrado en Ciencia e Ingeniería de la Computación Universidad Nacional Autónoma de México México City México

This research is framed within the affective computing, which explains the importance of emotions in human cognition (decision making, perception, interaction and human intelligence). Applying this approach to a pedagogical agent is an essential part to enhance the effectiveness of the teaching-learning process of an intelligent learning system. This work focuses on the design of the inference engine that will give life to the interface, where the latter is represented by a pedagogical agent. The inference engine is based on an affective-motivational model. This model is implemented by using artificial intelligence technique called fuzzy cognitive maps.

关键词： Affective-Motivational Model Intelligent Learning System Pedagogical Agent Affective Computing Simulation of Emotions

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Dynamic Interaction through a Reactive Interface in Patients with Dementia, by Means of Cognitive Stimulation

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E-Health Telecommunication Systems and Networks 2015年第4期4卷 57-67页

作者： Ana Lilia Laureano-Cruces Martha Mora-torres Lourdes Sánchez-guerrero Javier Ramírez-Rodríguez Itzel Iraís Montiel-Bernal Eric Benjamín Allier-Pavia Departamento de Sistemas Universidad Autónoma Metropolitana-Azcapotzalco México City México Posgrado en Ciencia e Ingeniería de la Computación Universidad Nacional Autónoma de México México City México Escuela Superior de Cómputo-Instituto Politécnico Nacional México City México Posgrado en Diseno Línea: Visualización de la Información Universidad Autónoma Metropolitana-Azcapotzalco México City México

Dementia is a syndrome of chronic and progressive nature, characterized by the deterioration of cognitive functions and behavior, which causes a disability and dependence on others. Alzheimer’s Disease is a type of dementia that affects cognitive functioning, behavior, and overall functionality of those who suffer from the irremediably progressive form, as it is a neurodegenerative disease. For this reason, development of a reactive interface is proposed to be inserted in a cognitive stimulation program. This program offers a series of exercises of mental functions, in order to delay or slow down the progress of this illness. The reactive interface allows access to these exercises and incorporates a user profile that predicts the possible emotions that may be experienced during the work sessions. In order to accomplish this, a cognitive structure of emotions is developed to be included in the reactive profile. This will allow enhancement of the interaction between the system and the patient. The user’s emotional state may interfere, considering within the design stage the phenomenon of altered consciousness during this degenerative process. The consciousness represents the interface of human beings with our environment. This proposal allows us to obtain information related to the user’s emotional state during the performance of specific cognitive tasks, permitting us to optimize the proposed stimulation activities.

关键词： Dementia Alzheimer Cognitive Stimulation Artificial Consciousness Cognitive Structure of the Emotions

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Intracerebral Microdialysis in Neurosurgery for Obsessive-Compulsive Disorder: Report of 2 Preliminary Cases

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Open Journal of Modern Neurosurgery 2020年第3期10卷 371-381页

作者： Sergio A. Sacchettoni Rafael Galera Juan F. Del Corral Juan P. Abud Aníbal Pinero Luis Teneud Napoleón torres Rafael Rojas Pedro Rada Félix guerrero Edgar González Mónica Bifano Dely Yazawa Luis Hernández Hospital and School of Medicine JM Vargas Universidad Central de Venezuela Caracas Venezuela Centro Medico-Docente La Trinidad Caracas Venezuela Neurology Mobile System Associates Miami FL USA Laboratory of Physiology of Behavior Universidad de Los Andes Mérida Venezuela University of Grenoble-Alpes CEA LETI Clinatec France

Neurosurgery for psychiatric disorders, notably for obsessive-compulsive disorder (OCD), was initiated in Venezuela in the decade of 1970, and consisted since that time in the classic stereotactic anterior cingulotomy. In order to know further about the physiopathology of this disorder, we performed intracerebral microdialysis in 2 patients who were operated on. The aim was to measure changes in extracellular neurotransmitters within the basal ganglia. The microdialysis probes were stereotactically placed in the right caudate nucleus and in the dorsomedial nucleus of the right thalamus. The microdialysis was done before the left cingulotomy, during the pause and after the right cingulotomy. Glutamate and gamma-aminobutyric acid (GABA) changes were similar in the caudate nucleus of both patients, whereas in the dorsomedial nucleus the changes were opposite among the 2 patients. Although this study does not bring enough data to explain such differences yet, the existence of dynamic changes in the neurochemistry of the basal ganglia during cingulotomy shows that intracerebral microdialysis can help in the understanding of the pathophysiology of OCD and eventually in the design of new surgeries with better results.

关键词： Psychiatric Neurosurgery Psychosurgery Obsessive-Compulsive Disorder Intracerebral Microdialysis

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