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Enhancement of Ethanol Production from Napiergrass (<i>Pennisetum purpureum</i>Schumach) by a Low-Moisture Anhydrous Ammonia Pretreatment

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Journal of Sustainable Bioenergy Systems 2013年第3期3卷 179-185页

作者： Masahide Yasuda Keisuke Takeo hayato nagai Takuya Uto Toshifumi Yui Tomoko Matsumoto Yasuyuki Ishii Kazuyoshi Ohta Center for Collaborative Research and Community Cooperation University of Miyazaki Miyazaki Japan Department of Animal and Grassland Sciences Faculty of Agriculture University of Miyazaki Miyazaki Japan Department of Applied Chemistry Faculty of Engineering University of Miyazaki Miyazaki Japan Department of Biochemistry and Applied Biosciences Faculty of Agriculture University of Miyazaki Miyazaki Japan

Napiegrass (Pennisetum purpureum Schumach) was treated with a low-moisture anhydrous ammonia (LMAA) pretreatment by adding an equal weight of water and keeping it under atmospheric ammonia gas at room temperature for four weeks. After the removal of ammonia and washing with water, a simultaneous saccharification and fermentation (SSF) was conducted for the LMAA-pretreated napiergrass (1.33 g) in a buffer solution (8 mL) using a mixture of a cellulase (80 mg) and a xylanase (53 mg) as well as the cell suspension (0.16 mL) of Saccharomyces cerevisiae. Ethanol and xylose resulted in 91.2% and 62.9% yields, respectively. The SSF process was scaled up using LMAA-pretreated napiergrass (100.0 g) to give ethanol (77.2%) and xylose (52.8%). After the removal of ethanol, the pentose fermentation of the SSF solution (40 mL), which contained 1.00 g of xylose, using cell suspension of Escherichia coli KO11 (70 mL) gave 86.3% yield of ethanol. Total ethanol yield reached 68.9% based on xylan (21.4 wt%) and glucan (39.7 wt%) of the LMAA-pretreated napiergrass.

关键词： LMAA SSF Cellulase Xylanase Saccharomyces cerevisiae Escherichia coli KO11

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Overexpression of c-met in the early stage of pancreatic carcinogenesis; altered expression is not sufficient for progression from chronic pancreatitis to pancreatic cancer

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World Journal of Gastroenterology 2006年第24期12卷 3878-3882页

作者： Kenoki Ohuchida Kazuhiro Mizumoto Nami Ishikawa Yasuhiro Ogura Daisuke Yamada Takuya Egami hayato Fujita Seiji Ohashi Eishi nagai Masao Tanaka Department of Surgery and Oncology Graduate School of Medical Sciences Kyushu UniversityFukuokaJapan

AIM： To investigate c-met expression during early pancreatic carcinogenesis. METHODS： We used 46 bulk tissues and 36 microdissected samples, including normal pancreas, chronic pancreatitis, and pancreatic cancer, for quantitative realtime reverse transcription-polymerase chain reaction. RESULTS： In bulk tissue analyses, pancreatic cancer tissues expressed significantly higher levels of c-met than did chronic pancreatitis and normal pancreas tissues. c-met levels did not differ between chronic pancreatitis and normal pancreas tissues. In microdissection-based analyses, c-met was expressed at higher levels in microclissected pancreatic cancer cells and pancreatitisaffected epithelial cells than in normal ductal epithelial cells （both, P 〈 0.01）. Interestingly, pancreatitis-affected epithelial cells expressed levels of c-met similar to those of pancreatic cancer cells. CONCLUSION： Overexpression of c-met occurs during the early stage of pancreatic carcinogenesis, and a single alteration of c-met expression is not sufficient for progression of chronic pancreatitis-affected epithelial cells to pancreatic cancer cells.

关键词： c-met', Pancreatic cancer Chronic pancreatitis Pancreatic carcinogenesis

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